Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,630,661 A→T 13.6% intergenic (‑59/+307) yhiI ← / ← yhiJ putative membrane fusion protein (MFP) of efflux pump/DUF4049 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,630,6610AT13.6% 308.5 / 36.7 103intergenic (‑59/+307)yhiI/yhiJputative membrane fusion protein (MFP) of efflux pump/DUF4049 family protein
Reads supporting (aligned to +/- strand):  ref base A (45/44);  new base T (7/7);  total (52/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

Only 100 of 103 total aligned reads displayed.

GCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAA  >  NC_000913/3630527‑3630801
                                                                                                                                      |                                                                                                                                            
gCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGa                                                                                                                                   >  2:140170/1‑146 (MQ=255)
gCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGa                                                                                                                                   <  1:140170/146‑1 (MQ=255)
 ccaccaCGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGAttc                                                                                                                                >  3:433495/1‑148 (MQ=255)
   accacGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGc                                                                                                                                      >  4:1114798/1‑140 (MQ=255)
   accacGCCACGATAGCCGCCACCCCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGc                                                                                                                                      <  3:1114798/140‑1 (MQ=255)
                        ccgccaGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATaaga                                                                                                               >  2:292904/1‑142 (MQ=255)
                        ccgccaGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATaaga                                                                                                               <  1:292904/142‑1 (MQ=255)
                                  cccGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAAtgc                                                                                               >  1:184268/1‑146 (MQ=255)
                                     gACAACCCACTACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCGTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATaaga                                                                                                               >  1:914964/1‑129 (MQ=255)
                                     gACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATaaga                                                                                                               <  2:914964/129‑1 (MQ=255)
                                      aCAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATaaga                                                                                                               >  2:43995/1‑128 (MQ=255)
                                      aCAACCCACCACGCCAAATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATaaga                                                                                                               <  1:43995/128‑1 (MQ=255)
                                        aaCCCCCCAGGCCATATGGCGTTTACTTTTATCCATATTCCACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTACAGCCGGGATAAGAAGGAAACGGTAAATACGACCAg                                                                                         <  1:158944/148‑1 (MQ=255)
                                             accacGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGATACg                                                                                                       >  3:949255/1‑129 (MQ=255)
                                              ccacGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGt                                                                                                                                           <  3:1045334/92‑1 (MQ=255)
                                              ccacGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGt                                                                                                                                           >  4:1045334/1‑92 (MQ=255)
                                               cacGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATGCGACCAGCGCGCag                                                                                  <  2:184268/148‑1 (MQ=255)
                                                 cGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACg                                                                                              >  2:730068/1‑134 (MQ=255)
                                                 cGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACg                                                                                              <  1:730068/134‑1 (MQ=255)
                                                    cAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCAt                                                                             <  4:433495/148‑1 (MQ=255)
                                                       aTGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAAc                                                                                                        >  1:243218/1‑118 (MQ=255)
                                                       aTGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAAc                                                                                                        <  2:243218/118‑1 (MQ=255)
                                                             cTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCa                                                                    >  4:742378/1‑148 (MQ=255)
                                                                   ctTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCg                                                                                                                       >  1:541147/1‑91 (MQ=255)
                                                                   ctTACCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCg                                                                                                                       <  2:541147/91‑1 (MQ=255)
                                                                     tATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAgg                                                                                                            >  4:341904/1‑100 (MQ=255)
                                                                     tATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAgg                                                                                                            <  3:341904/100‑1 (MQ=255)
                                                                     tATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCGAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGaa                                                            <  3:742378/148‑1 (MQ=255)
                                                                     tATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAg                                                                                         <  2:528762/119‑1 (MQ=255)
                                                                     tATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAg                                                                                         >  1:528762/1‑119 (MQ=255)
                                                                     tATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTg                                                                           <  3:585850/133‑1 (MQ=255)
                                                                     tATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTg                                                                           >  4:585850/1‑133 (MQ=255)
                                                                     tATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGaa                                                            >  4:1072944/1‑148 (MQ=255)
                                                                          aTAATCGACCCGCCATAATCCCTGTACGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCg                                                                                                                       >  2:771925/1‑84 (MQ=255)
                                                                          aTAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGaa                                                                                                          <  3:13404/97‑1 (MQ=255)
                                                                          aTAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGaa                                                                                                          >  4:13404/1‑97 (MQ=255)
                                                                          aTAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATa                                                                                                >  1:1065970/1‑107 (MQ=255)
                                                                          aTAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATa                                                                                                <  2:1065970/107‑1 (MQ=255)
                                                                          aTAACCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCg                                                                                                                       <  1:771925/84‑1 (MQ=255)
                                                                           tAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACggg                                                               <  2:597609/139‑1 (MQ=255)
                                                                           tAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACggg                                                               >  1:597609/1‑139 (MQ=255)
                                                                              tCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATaagaa                                                                                                              >  3:167985/1‑89 (MQ=255)
                                                                              tCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATaagaa                                                                                                              <  4:167985/89‑1 (MQ=255)
                                                                               cGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATaag                                                                                                                <  2:1015631/86‑1 (MQ=255)
                                                                               cGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATaag                                                                                                                >  1:1015631/1‑86 (MQ=255)
                                                                                  cccGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGaaaa                                               >  2:342301/1‑148 (MQ=255)
                                                                                   ccGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGaaaa                                               >  2:363895/1‑147 (MQ=255)
                                                                                   ccGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGaaaa                                               <  1:363895/147‑1 (MQ=255)
                                                                                    cGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGa                                                             <  2:469882/132‑1 (MQ=255)
                                                                                    cGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGa                                                             >  1:469882/1‑132 (MQ=255)
                                                                                    cGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCtt                                                       <  3:615963/138‑1 (MQ=255)
                                                                                    cGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCtt                                                       >  4:615963/1‑138 (MQ=255)
                                                                                     gCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATaag                                                                                                                <  4:368093/80‑1 (MQ=255)
                                                                                      ccATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCggg                                                                                                                     >  1:1116363/1‑74 (MQ=255)
                                                                                      ccATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCggg                                                                                                                     <  2:1116363/74‑1 (MQ=255)
                                                                                            tCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTaa                                     <  3:1072944/148‑1 (MQ=255)
                                                                                              ccTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACg                                             >  4:1085101/1‑138 (MQ=255)
                                                                                              ccTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACg                                             <  3:1085101/138‑1 (MQ=255)
                                                                                              ccTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGATCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGc                                                                               >  4:702424/1‑104 (MQ=255)
                                                                                              ccTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACCACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGc                                                                               <  3:702424/104‑1 (MQ=255)
                                                                                                 gTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGAttct                                                                                                                               <  1:743130/53‑1 (MQ=255)
                                                                                                 gTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGAttct                                                                                                                               >  2:743130/1‑53 (MQ=255)
                                                                                                 gTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCa                                                                                          <  4:751556/90‑1 (MQ=255)
                                                                                                 gTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCa                                                                                          >  3:751556/1‑90 (MQ=255)
                                                                                                     gCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGtcat                            >  1:431386/1‑148 (MQ=255)
                                                                                                        aaCGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATa                                                                                                >  4:22470/1‑77 (MQ=255)
                                                                                                        aaCGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATa                                                                                                <  3:22470/77‑1 (MQ=255)
                                                                                                        aaCGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAg                                                           >  2:113909/1‑114 (MQ=255)
                                                                                                        aaCGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAg                                                           <  1:113909/114‑1 (MQ=255)
                                                                                                        aaCGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGtcatcat                         >  2:672510/1‑148 (MQ=255)
                                                                                                               cgTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCAtt                  <  1:342301/148‑1 (MQ=255)
                                                                                                                 tgtTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTcc                >  2:893707/1‑148 (MQ=255)
                                                                                                                    tACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAgcgcgc                                                                                    >  3:20140/1‑77 (MQ=255)
                                                                                                                    tACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAgcgcgc                                                                                    <  4:20140/77‑1 (MQ=255)
                                                                                                                    tACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAAtgttgt                               >  2:133160/1‑130 (MQ=255)
                                                                                                                    tACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAAtgttgt                               <  1:133160/130‑1 (MQ=255)
                                                                                                                     aCAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGc            >  3:490062/1‑148 (MQ=255)
                                                                                                                      cAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGcc           <  4:490062/148‑1 (MQ=255)
                                                                                                                      cAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGcc           >  1:766139/1‑148 (MQ=255)
                                                                                                                        aCTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGtcatca                          >  2:244798/1‑131 (MQ=255)
                                                                                                                        aCTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGtcatca                          <  1:244798/131‑1 (MQ=255)
                                                                                                                        aCTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGt         <  1:672510/148‑1 (MQ=255)
                                                                                                                           tccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGtcat                            >  1:265863/2‑126 (MQ=255)
                                                                                                                           tccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGtcat                            <  2:265863/125‑1 (MQ=255)
                                                                                                                            ccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCag                                                                                  >  3:778419/1‑71 (MQ=38)
                                                                                                                            ccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCag                                                                                  <  4:778419/71‑1 (MQ=39)
                                                                                                                            ccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTa                                                      <  2:551073/99‑1 (MQ=255)
                                                                                                                            ccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTa                                                      >  1:551073/1‑99 (MQ=255)
                                                                                                                            ccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAg                                                     >  1:129050/1‑100 (MQ=255)
                                                                                                                            ccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGgt                                          >  2:164718/1‑111 (MQ=255)
                                                                                                                            ccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGgt                                          <  1:164718/111‑1 (MQ=255)
                                                                                                                             cgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAgcg                                                                       <  2:311755/81‑1 (MQ=255)
                                                                                                                             cgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAgcg                                                                       >  1:311755/1‑81 (MQ=255)
                                                                                                                              gctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGgtagt                                       <  3:608569/109‑1 (MQ=255)
                                                                                                                              gctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGgtagt                                       >  4:608569/4‑112 (MQ=255)
                                                                                                                               ccgAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAAtgttgt                               <  3:723362/119‑1 (MQ=255)
                                                                                                                               ccgAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAAtgttgt                               >  4:723362/1‑119 (MQ=255)
                                                                                                                               ccgAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGaa  <  2:431386/148‑1 (MQ=255)
                                                                                                                                cgAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCtt                                                       <  3:817387/94‑1 (MQ=255)
                                                                                                                                cgAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCtt                                                       >  4:817387/1‑94 (MQ=255)
                                                                                                                                      |                                                                                                                                            
GCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAA  >  NC_000913/3630527‑3630801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: