Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,636,007 A→C 7.8% intergenic (+114/+201) yhiM → / ← yhiN acid resistance protein, inner membrane/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,636,0070AC 7.8% 173.0 / 6.3 51intergenic (+114/+201)yhiM/yhiNacid resistance protein, inner membrane/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (24/23);  new base C (2/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

CGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATCACAA  >  NC_000913/3635900‑3636140
                                                                                                           |                                                                                                                                     
cGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATc                                                                                               >  3:570233/1‑148 (MQ=255)
      ggATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCa                                                                                         <  4:570233/148‑1 (MQ=255)
             aCCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTa                                                                                                                                   >  1:184706/1‑99 (MQ=255)
             aCCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTa                                                                                                                                   <  2:184706/99‑1 (MQ=255)
                    ttGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACtgagtg                                                                                  >  1:1071850/1‑141 (MQ=255)
                    ttGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACtgagtg                                                                                  <  2:1071850/141‑1 (MQ=255)
                           tttGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATc                                                                                               >  4:842835/1‑121 (MQ=255)
                           tttGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATc                                                                                               <  3:842835/121‑1 (MQ=255)
                            ttGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGTAACTTTGCTGttt                                                                                                                             >  1:423942/1‑90 (MQ=255)
                            ttGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGTAACTTTGCTGttt                                                                                                                             <  2:423942/90‑1 (MQ=255)
                                      cTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTtccatc                                                            <  4:212226/145‑1 (MQ=255)
                                      cTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTtccatc                                                            >  3:212226/1‑145 (MQ=255)
                                           ttttCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGtt                                                                                                                                    <  3:401549/68‑1 (MQ=255)
                                           ttttCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGtt                                                                                                                                    >  4:401549/1‑68 (MQ=255)
                                            tttCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCt                                                                                              <  1:252873/105‑1 (MQ=255)
                                            tttCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCt                                                                                              >  2:252873/1‑105 (MQ=255)
                                                 tAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGtt                                                                                                                                    >  3:437148/1‑62 (MQ=255)
                                                 tAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGtt                                                                                                                                    <  4:437148/62‑1 (MQ=255)
                                                        catGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGtt                                                                                                                <  1:1007457/75‑1 (MQ=255)
                                                        catGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGtt                                                                                                                >  2:1007457/1‑75 (MQ=255)
                                                         atGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGttt                                                                             <  2:375517/109‑1 (MQ=255)
                                                         atGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGttt                                                                             >  1:375517/1‑109 (MQ=255)
                                                         atGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTtc                                                                >  2:266608/1‑122 (MQ=255)
                                                         atGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTtc                                                                <  1:266608/122‑1 (MQ=255)
                                                          tGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGtttttt                                                                                                                          <  4:450527/63‑1 (MQ=255)
                                                          tGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGtttttt                                                                                                                          >  3:450527/1‑63 (MQ=255)
                                                          tGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCa                                                                                                                       >  2:684334/1‑66 (MQ=255)
                                                          tGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCa                                                                                                                       <  1:684334/66‑1 (MQ=255)
                                                          tGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTg                                                                                             <  4:253405/92‑1 (MQ=255)
                                                          tGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTg                                                                                             >  3:253405/1‑92 (MQ=255)
                                                           gAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGtt                                                                              >  3:899808/1‑106 (MQ=255)
                                                           gAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGtt                                                                              <  4:899808/106‑1 (MQ=255)
                                                               tAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACtgagtgag                                                                                <  3:29940/100‑1 (MQ=255)
                                                               tAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACtgagtgag                                                                                >  4:29940/1‑100 (MQ=255)
                                                                       caTTAATGCATAGTGGTTAAGTATAAGAAACAGCAAAGTTACTGTTTTTTTCAACCTGt                                                                                                                 <  3:534098/59‑1 (MQ=255)
                                                                       caTTAATGCATAGTGGTTAAGTATAAGAAACAGCAAAGTTACTGTTTTTTTCAACCTGt                                                                                                                 >  4:534098/1‑59 (MQ=255)
                                                                                 tAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGa              >  2:685483/1‑148 (MQ=255)
                                                                                  aGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTACAGTTCACGGGCTCATTAGaa             <  1:685483/148‑1 (MQ=255)
                                                                                   gTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCAt                                           <  3:466253/117‑1 (MQ=255)
                                                                                   gTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCAt                                           >  4:466253/1‑117 (MQ=255)
                                                                                             ataAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATCACaa  >  1:501622/1‑148 (MQ=255)
                                                                                               aaaaaaCAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCa                                                                          >  4:311789/1‑74 (MQ=255)
                                                                                               aaaaaaCAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCa                                                                          <  3:311789/74‑1 (MQ=255)
                                                                                               aaaaaaCAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAaataa        >  3:633253/1‑140 (MQ=255)
                                                                                               aaaaaaCAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAaataa        <  4:633253/140‑1 (MQ=255)
                                                                                                  aaaCAGTAACTTTGCTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTaa                                               <  2:730023/98‑1 (MQ=255)
                                                                                                  aaaCAGTAACTTTGCTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTaa                                               >  1:730023/1‑98 (MQ=255)
                                                                                                  aaaCAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCtt                                                      >  3:574815/1‑91 (MQ=255)
                                                                                                  aaaCAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCtt                                                      <  4:574815/91‑1 (MQ=255)
                                                                                                    aCAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGtt                                   >  2:522407/1‑108 (MQ=255)
                                                                                                    aCAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGtt                                   <  1:522407/108‑1 (MQ=255)
                                                                                                           aGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCAt                                           <  4:414397/93‑1 (MQ=255)
                                                                                                           aGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCAt                                           >  3:414397/1‑93 (MQ=255)
                                                                                                           |                                                                                                                                     
CGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATCACAA  >  NC_000913/3635900‑3636140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: