| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,636,007 | A→C | 7.8% | intergenic (+114/+201) | yhiM → / ← yhiN | acid resistance protein, inner membrane/putative oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,636,007 | 0 | A | C | 7.8% | 173.0 / 6.3 | 51 | intergenic (+114/+201) | yhiM/yhiN | acid resistance protein, inner membrane/putative oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (24/23); new base C (2/2); total (26/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
CGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATCACAA > NC_000913/3635900‑3636140 | cGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATc > 3:570233/1‑148 (MQ=255) ggATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCa < 4:570233/148‑1 (MQ=255) aCCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTa > 1:184706/1‑99 (MQ=255) aCCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTa < 2:184706/99‑1 (MQ=255) ttGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACtgagtg > 1:1071850/1‑141 (MQ=255) ttGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACtgagtg < 2:1071850/141‑1 (MQ=255) tttGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATc > 4:842835/1‑121 (MQ=255) tttGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATc < 3:842835/121‑1 (MQ=255) ttGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGTAACTTTGCTGttt > 1:423942/1‑90 (MQ=255) ttGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGTAACTTTGCTGttt < 2:423942/90‑1 (MQ=255) cTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTtccatc < 4:212226/145‑1 (MQ=255) cTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTtccatc > 3:212226/1‑145 (MQ=255) ttttCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGtt < 3:401549/68‑1 (MQ=255) ttttCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGtt > 4:401549/1‑68 (MQ=255) tttCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCt < 1:252873/105‑1 (MQ=255) tttCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCt > 2:252873/1‑105 (MQ=255) tAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGtt > 3:437148/1‑62 (MQ=255) tAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGtt < 4:437148/62‑1 (MQ=255) catGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGtt < 1:1007457/75‑1 (MQ=255) catGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGtt > 2:1007457/1‑75 (MQ=255) atGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGttt < 2:375517/109‑1 (MQ=255) atGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGttt > 1:375517/1‑109 (MQ=255) atGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTtc > 2:266608/1‑122 (MQ=255) atGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTtc < 1:266608/122‑1 (MQ=255) tGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGtttttt < 4:450527/63‑1 (MQ=255) tGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGtttttt > 3:450527/1‑63 (MQ=255) tGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCa > 2:684334/1‑66 (MQ=255) tGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCa < 1:684334/66‑1 (MQ=255) tGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTg < 4:253405/92‑1 (MQ=255) tGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTg > 3:253405/1‑92 (MQ=255) gAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGtt > 3:899808/1‑106 (MQ=255) gAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGtt < 4:899808/106‑1 (MQ=255) tAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACtgagtgag < 3:29940/100‑1 (MQ=255) tAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACtgagtgag > 4:29940/1‑100 (MQ=255) caTTAATGCATAGTGGTTAAGTATAAGAAACAGCAAAGTTACTGTTTTTTTCAACCTGt < 3:534098/59‑1 (MQ=255) caTTAATGCATAGTGGTTAAGTATAAGAAACAGCAAAGTTACTGTTTTTTTCAACCTGt > 4:534098/1‑59 (MQ=255) tAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGa > 2:685483/1‑148 (MQ=255) aGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTACAGTTCACGGGCTCATTAGaa < 1:685483/148‑1 (MQ=255) gTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCAt < 3:466253/117‑1 (MQ=255) gTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCAt > 4:466253/1‑117 (MQ=255) ataAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATCACaa > 1:501622/1‑148 (MQ=255) aaaaaaCAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCa > 4:311789/1‑74 (MQ=255) aaaaaaCAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCa < 3:311789/74‑1 (MQ=255) aaaaaaCAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAaataa > 3:633253/1‑140 (MQ=255) aaaaaaCAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAaataa < 4:633253/140‑1 (MQ=255) aaaCAGTAACTTTGCTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTaa < 2:730023/98‑1 (MQ=255) aaaCAGTAACTTTGCTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTaa > 1:730023/1‑98 (MQ=255) aaaCAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCtt > 3:574815/1‑91 (MQ=255) aaaCAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCtt < 4:574815/91‑1 (MQ=255) aCAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGtt > 2:522407/1‑108 (MQ=255) aCAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGtt < 1:522407/108‑1 (MQ=255) aGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCAt < 4:414397/93‑1 (MQ=255) aGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCAt > 3:414397/1‑93 (MQ=255) | CGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATCACAA > NC_000913/3635900‑3636140 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |