| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,697,970 | T→C | 5.4% | W505R (TGG→CGG) | bcsE → | cellulose production protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,697,970 | 0 | T | C | 5.4% | 252.4 / 3.4 | 74 | W505R (TGG→CGG) | bcsE | cellulose production protein |
| Reads supporting (aligned to +/- strand): ref base T (35/35); new base C (2/2); total (37/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01 | |||||||||||
GGTTTGAAGATGATCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTTTTCCCGCTGGGCTATCTGGCGCGGCACTCTTTG > NC_000913/3697839‑3698112 | ggTTTGAAGATGATCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTgg < 2:576142/134‑1 (MQ=255)ggTTTGAAGATGATCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTgg > 1:576142/1‑134 (MQ=255) gatgatCAAATCAGTGCCGAGCTGGTGCAGATGCGTTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGc > 4:1080100/1‑148 (MQ=255) atCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGa > 4:805146/1‑136 (MQ=255) atCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGa < 3:805146/136‑1 (MQ=255) tCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCa < 1:681817/140‑1 (MQ=255) tCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCa > 2:681817/1‑140 (MQ=255) aaaTCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCTCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGtt < 3:1080100/148‑1 (MQ=255) ccGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGc > 2:650268/1‑148 (MQ=255) aGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAg < 2:27561/138‑1 (MQ=255) aGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAg > 1:27561/1‑138 (MQ=255) cTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACt < 1:893539/103‑1 (MQ=255) cTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACt > 2:893539/1‑103 (MQ=255) tGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCAGTGGcg > 1:969105/1‑103 (MQ=255) tGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCAGTGGcg < 2:969105/103‑1 (MQ=255) tGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTa > 1:481613/1‑131 (MQ=255) tGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTa < 2:481613/131‑1 (MQ=255) gATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCATAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGcga > 2:80850/1‑100 (MQ=255) gATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCATAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGcga < 1:80850/100‑1 (MQ=255) cgcTTGCTTGCCCCAGAACAGTGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAg > 4:733770/1‑124 (MQ=255) cgcTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAg < 3:733770/124‑1 (MQ=255) cttgcCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTgg > 2:1025248/1‑87 (MQ=255) cttgcCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTgg < 1:1025248/87‑1 (MQ=255) cttgcCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATAc < 3:126794/97‑1 (MQ=255) cttgcCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATAc > 4:126794/1‑97 (MQ=255) cttgcCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATg < 1:424486/108‑1 (MQ=255) cttgcCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATg > 2:424486/1‑108 (MQ=255) cAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGt < 1:60608/108‑1 (MQ=255) cAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGt > 2:60608/1‑108 (MQ=255) cAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCtcatc > 2:934412/1‑133 (MQ=255) cAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCtcatc < 1:934412/133‑1 (MQ=255) cAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGc > 4:346654/1‑148 (MQ=255) aCAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATAcc < 3:873160/87‑1 (MQ=255) aCAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATAcc > 4:873160/1‑87 (MQ=255) ggggCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGt > 1:1044095/1‑99 (MQ=255) ggggCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGt < 2:1044095/99‑1 (MQ=255) ggCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGa < 1:650268/148‑1 (MQ=255) cATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCAt < 2:496195/143‑1 (MQ=255) cATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCAt > 1:496195/1‑143 (MQ=255) cTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGtt < 3:346654/148‑1 (MQ=255) tttAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATa > 1:1084817/1‑64 (MQ=255) tttAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATa < 2:1084817/64‑1 (MQ=255) ttAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCt > 3:667382/1‑103 (MQ=255) ttAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCt < 4:667382/103‑1 (MQ=255) ttAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCAtt > 1:40836/1‑131 (MQ=255) ttAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCAtt < 2:40836/131‑1 (MQ=255) ttAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACCGGCGACGAATACCAGAccc < 3:439032/71‑1 (MQ=255) ttAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACCGGCGACGAATACCAGAAcc < 1:489235/71‑1 (MQ=255) ttAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACCGGCGACGAATACCAGAAcc > 4:439032/1‑71 (MQ=255) ttAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACCGGCGACGAATACCAGAAcc > 2:489235/1‑71 (MQ=255) cAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTg < 4:64940/75‑1 (MQ=255) cAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTg > 3:64940/1‑75 (MQ=255) cAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGc > 4:28382/1‑116 (MQ=255) cAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGc < 3:28382/116‑1 (MQ=255) aaGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCa < 3:252799/121‑1 (MQ=255) aaGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCa > 4:252799/1‑121 (MQ=255) aaGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGaa > 2:656814/1‑126 (MQ=255) aaGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGaa < 1:656814/126‑1 (MQ=255) tCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAgcgc < 2:66915/90‑1 (MQ=255) tCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAgcgc > 1:66915/1‑90 (MQ=255) tCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAg > 3:511369/1‑109 (MQ=255) tCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAg < 4:511369/109‑1 (MQ=255) aaaCCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGt > 1:992602/1‑67 (MQ=255) aaaCCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGt < 2:992602/67‑1 (MQ=255) aCCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAgcgc < 2:911744/85‑1 (MQ=255) aCCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAgcgc > 1:911744/1‑85 (MQ=255) aCCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGa > 3:1035992/1‑116 (MQ=255) aCCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGa < 4:1035992/116‑1 (MQ=255) cACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAatta > 2:382674/1‑102 (MQ=255) cACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAatta < 1:382674/102‑1 (MQ=255) cACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCa > 2:242680/1‑104 (MQ=255) cACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCa < 1:242680/104‑1 (MQ=255) aCTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTTTTCCCGCTgg > 2:1005345/1‑122 (MQ=255) aCTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTTTTCCCGCTgg < 1:1005345/122‑1 (MQ=255) aCTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTTTTCCCGCTGGGCTATCTGGCGCGGCACTCTTTg < 4:867279/145‑1 (MQ=255) aCTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTTTTCCCGCTGGGCTATCTGGCGCGGCACTCTTTg > 3:867279/1‑145 (MQ=255) | GGTTTGAAGATGATCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTTTTCCCGCTGGGCTATCTGGCGCGGCACTCTTTG > NC_000913/3697839‑3698112 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |