Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,740,791 G→C 5.9% G363R (GGC→CGC)  avtA → valine‑pyruvate aminotransferase; transaminase C; alanine‑valine transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,740,7910GC 5.9% 1304.6 / 53.1 357G363R (GGC→CGC) avtAvaline‑pyruvate aminotransferase; transaminase C; alanine‑valine transaminase
Reads supporting (aligned to +/- strand):  ref base G (170/166);  new base C (10/11);  total (180/177)
Fisher's exact test for biased strand distribution p-value = 8.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.30e-01

Only 100 of 372 total aligned reads displayed.

GGAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGA  >  NC_000913/3740649‑3740937
                                                                                                                                              |                                                                                                                                                  
ggAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCAcgcg                                                                                                                                                    >  3:559466/1‑143 (MQ=255)
       aTCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTg                                                                                                                                               <  4:447623/141‑1 (MQ=255)
                     ccgcTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCggg                                                                                                                                 >  2:1105129/1‑141 (MQ=255)
                          aTTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAActtctt                                                                                                                     <  1:752554/148‑1 (MQ=255)
                                cGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCgggg                                                                                                                                >  4:292874/1‑131 (MQ=255)
                                 ggAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAggg                                                                                                              >  4:15256/1‑148 (MQ=255)
                                 ggAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAggg                                                                                                              >  4:41099/1‑148 (MQ=255)
                                   aaaaTCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCg                                                                                                                                   >  4:702319/1‑125 (MQ=255)
                                   aaaaTCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCg                                                                                                                                   <  3:702319/125‑1 (MQ=255)
                                       tCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGAt                                                                                                        >  2:219596/1‑148 (MQ=255)
                                        cGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATgg                                                                                                                                        >  1:634946/1‑115 (MQ=255)
                                          cTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATaaa                                                                                                     <  3:15256/148‑1 (MQ=255)
                                                  ttCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGAt                                                                                                                                          <  1:714759/103‑1 (MQ=255)
                                                       aaaCCGGAAGGAGCCATTTTCCTCTGGCTCTGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGCGCCGGGGCACAActtc                                                                                                                       <  3:787525/117‑1 (MQ=255)
                                                       aaaCCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAActtc                                                                                                                       <  2:756295/117‑1 (MQ=255)
                                                       aaaCCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATa                                                                                        <  2:132542/148‑1 (MQ=255)
                                                       aaaCCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACAACCAAGCAGCTATATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGCGCCGGGGCACAActtc                                                                                                                       >  4:787525/1‑117 (MQ=255)
                                                          ccGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTg                                                                                                           >  1:737725/1‑126 (MQ=255)
                                                           cGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAAc                                                                                                    >  4:378125/1‑132 (MQ=255)
                                                            ggAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATgg                                                                                                                                        <  3:1020618/95‑1 (MQ=255)
                                                            ggAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATgg                                                                                                                                        >  4:1020618/1‑95 (MQ=255)
                                                              aaGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTgg                                                                                                          >  4:480857/1‑123 (MQ=255)
                                                               aGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCa                                                                                    <  2:734135/144‑1 (MQ=255)
                                                                  aGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGt                                                                             >  1:685074/1‑148 (MQ=255)
                                                                   gCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTa                                                                            >  1:1031855/1‑148 (MQ=255)
                                                                     cATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGa                                                                                                                                           <  4:397564/83‑1 (MQ=255)
                                                                       ttttCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACgcggcg                                                                                                                                                 <  3:378713/75‑1 (MQ=255)
                                                                       ttttCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTg                                                                                                >  4:320761/1‑124 (MQ=255)
                                                                       ttttCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATgcg                                                                        >  4:271442/1‑148 (MQ=255)
                                                                        tttCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATgg                                                                                                                                        <  2:414009/83‑1 (MQ=255)
                                                                            ctctGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGa                                                                   <  3:271442/148‑1 (MQ=255)
                                                                             tctGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGaa                                                                  <  2:1031855/148‑1 (MQ=255)
                                                                              ctGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGc                                                                                     <  3:1082237/128‑1 (MQ=255)
                                                                              ctGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGc                                                                                     >  4:1082237/1‑128 (MQ=255)
                                                                                   tATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGt                                                            <  3:909727/148‑1 (MQ=255)
                                                                                    aTGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGa                                                                                                                                           >  2:140478/1‑68 (MQ=255)
                                                                                    aTGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCa                                                                                    >  4:840514/1‑123 (MQ=255)
                                                                                    aTGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTa                                                           <  1:1067896/148‑1 (MQ=255)
                                                                                        tttAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCag                                                       <  3:397733/148‑1 (MQ=255)
                                                                                         ttAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTccc                                                                                                                  <  4:234469/88‑1 (MQ=255)
                                                                                         ttAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTccc                                                                                                                  >  3:234469/1‑88 (MQ=255)
                                                                                         ttAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATaaa                                                                                                     >  1:842875/1‑101 (MQ=255)
                                                                                         ttAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCaa                                                                                >  1:591663/1‑122 (MQ=255)
                                                                                          tAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGAt                                                                                                                                          >  4:521006/1‑63 (MQ=255)
                                                                                           aaGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGt                                                                             >  3:827286/1‑123 (MQ=255)
                                                                                           aaGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCa                                                                      >  1:1045766/1‑130 (MQ=255)
                                                                                           aaGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCa                                                                      <  2:1045766/130‑1 (MQ=255)
                                                                                              gATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCgg                                                 <  2:920773/148‑1 (MQ=255)
                                                                                               aTTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCa                                                                                          >  2:878343/1‑106 (MQ=255)
                                                                                                tttGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTgg                                                                                                          >  3:369706/1‑89 (MQ=255)
                                                                                                    cccATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGca                                                                                                                              >  1:852434/1‑65 (MQ=255)
                                                                                                    cccATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGca                                                                                                                              <  2:852434/65‑1 (MQ=255)
                                                                                                    cccATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGaaaa                                           >  2:18708/1‑148 (MQ=255)
                                                                                                      cATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTccc                                                                                                                  <  3:336831/75‑1 (MQ=255)
                                                                                                      cATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCaa                                                                                >  2:83489/1‑109 (MQ=255)
                                                                                                       aTTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATg                                                                          >  4:1106452/1‑114 (MQ=255)
                                                                                                       aTTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATg                                                                          <  3:1106452/114‑1 (MQ=255)
                                                                                                         tACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCaa                                                                                <  2:668890/106‑1 (MQ=255)
                                                                                                         tACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTg                                        >  2:244202/1‑146 (MQ=255)
                                                                                                         tACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAg                                      >  2:993602/1‑148 (MQ=255)
                                                                                                           cGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGcc                                                                                                                                    >  1:520761/1‑52 (MQ=255)
                                                                                                            gACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCg                                   <  2:514891/148‑1 (MQ=255)
                                                                                                             aCCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATaaa                                                                                                     <  4:938530/81‑1 (MQ=255)
                                                                                                              ccAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCAGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCggg                                 >  2:334528/1‑148 (MQ=255)
                                                                                                               cAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTgg                                                                                                          <  3:436803/74‑1 (MQ=255)
                                                                                                               cAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCgggg                                >  1:238664/1‑148 (MQ=255)
                                                                                                                aaGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGt                                                                             >  3:742729/1‑102 (MQ=255)
                                                                                                                aaGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCggggg                               >  3:245974/1‑148 (MQ=255)
                                                                                                                   cagcTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCgg                                                 <  1:1129444/127‑1 (MQ=255)
                                                                                                                   cagcTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGa                            >  4:619745/1‑148 (MQ=255)
                                                                                                                     gcTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAg                          >  2:598406/1‑148 (MQ=255)
                                                                                                                      ctctATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCaa                                                                                <  3:298137/93‑1 (MQ=255)
                                                                                                                       tctATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTg                                        <  3:629334/132‑1 (MQ=255)
                                                                                                                        ctATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTa                                                                            >  4:567336/1‑95 (MQ=255)
                                                                                                                        ctATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCa                                                                      <  3:487164/101‑1 (MQ=255)
                                                                                                                        ctATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCgg                                                 <  1:509449/122‑1 (MQ=255)
                                                                                                                          aTCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAAt                                          <  2:53801/127‑1 (MQ=255)
                                                                                                                          aTCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACGACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGa                         >  3:98295/1‑144 (MQ=255)
                                                                                                                                 ccTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTgg                                                                                                          >  1:924464/1‑56 (MQ=255)
                                                                                                                                 ccTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGt                                                            >  4:358021/1‑102 (MQ=255)
                                                                                                                                 ccTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTg                             >  1:394755/1‑133 (MQ=255)
                                                                                                                                   tGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGgaga                                              <  1:135278/114‑1 (MQ=255)
                                                                                                                                   tGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAaga            <  4:245974/148‑1 (MQ=255)
                                                                                                                                    atcaGCACGCCGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGc                                                    >  3:479602/4‑107 (MQ=255)
                                                                                                                                     tcaGCACGCCGCGTGCTGATGGTGCCGGGTCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAg                          >  1:786214/3‑132 (MQ=255)
                                                                                                                                     tcaGCACGCCGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCa                                                                                 >  4:64151/3‑77 (MQ=255)
                                                                                                                                     tcaGCACGCCGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCa                                                                                 <  3:64151/75‑1 (MQ=255)
                                                                                                                                     tcaGCACGCCGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAg                          <  2:786214/130‑1 (MQ=255)
                                                                                                                                     aaaGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCa                                                                      >  1:265068/1‑88 (MQ=255)
                                                                                                                                      caGCACGCCGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCAt                                                                                         <  3:183277/67‑1 (MQ=255)
                                                                                                                                      caGCACGCCGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCAt                                                                                         >  4:183277/2‑68 (MQ=255)
                                                                                                                                       aGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAAc                                                                 >  2:704292/1‑91 (MQ=255)
                                                                                                                                        gCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAg       >  2:286902/1‑148 (MQ=255)
                                                                                                                                        gCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAg       >  2:808116/1‑148 (MQ=255)
                                                                                                                                         cACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATg                                                                              >  1:150094/1‑76 (MQ=255)
                                                                                                                                         cACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGa               >  1:203828/1‑139 (MQ=255)
                                                                                                                                           cgcgGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGa                                       <  4:75275/113‑1 (MQ=255)
                                                                                                                                             cggcgTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGc                                                    <  1:890678/98‑1 (MQ=255)
                                                                                                                                             cggcgTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCggg                                 <  1:315344/117‑1 (MQ=255)
                                                                                                                                             cggcgTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAaga  <  1:812021/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
GGAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGA  >  NC_000913/3740649‑3740937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: