Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,756,564 T→A 5.1% intergenic (‑53/+112) aldB ← / ← yiaY aldehyde dehydrogenase B/L‑threonine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,756,5640TA 5.1% 787.2 / 21.2 212intergenic (‑53/+112)aldB/yiaYaldehyde dehydrogenase B/L‑threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (101/100);  new base A (6/5);  total (107/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

Only 100 of 217 total aligned reads displayed.

TCGCCGCCAATAAAGTTGTCATAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTT  >  NC_000913/3756417‑3756710
                                                                                                                                                    |                                                                                                                                                  
      ccAATAAAGTTGTCATAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTc                                                                                                                                                <  1:655627/146‑1 (MQ=255)
      ccAATAAAGTTATCATAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCg                                                                                                                                               >  1:861874/1‑147 (MQ=255)
               ttGTCATAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCtt                                                                                                                                                 >  2:955652/1‑136 (MQ=255)
               ttGTCATAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCtt                                                                                                                                                 <  1:955652/136‑1 (MQ=255)
                     tAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCtt                                                                                                                                                 >  1:290516/1‑130 (MQ=255)
                              tttAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGt                                                                                                                                          <  1:697083/128‑1 (MQ=255)
                              tttAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCttt                                                                                                                             <  1:511870/141‑1 (MQ=255)
                                     tGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCt                                                                                                                                                  >  4:748006/1‑113 (MQ=255)
                                         ggggAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCgg                                                                                                                                       <  2:1079785/120‑1 (MQ=255)
                                         ggggAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCgg                                                                                                                                       >  1:1079785/1‑120 (MQ=255)
                                          gggAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCtt                                                                                                                                                 >  4:513937/1‑109 (MQ=255)
                                           ggAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGCGACTGGCGAAGATTTCGcc                                                                                                                                             >  2:769654/1‑112 (MQ=255)
                                             aaaCCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTGACGACAGCTATCACGAATTTAc                                                                                                       >  1:813984/1‑148 (MQ=255)
                                               aCCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCtt                                                                                                                                                 <  2:617474/104‑1 (MQ=255)
                                                  aTACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTAt                                                                                                                  >  4:585100/1‑132 (MQ=255)
                                                   tACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCtt                                                                                                                                                 >  4:443811/1‑100 (MQ=255)
                                                   tACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCtt                                                                                                                                                 <  3:443811/100‑1 (MQ=255)
                                                   tACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTc                                                                                                                                                >  1:372039/1‑101 (MQ=255)
                                                   tACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTc                                                                                                                                                <  2:372039/101‑1 (MQ=255)
                                                   tACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGa                                                                                                             <  4:530762/136‑1 (MQ=255)
                                                   tACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTa                                                                                                        >  2:132407/1‑141 (MQ=255)
                                                    aCTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAg                                                                                                <  3:478659/148‑1 (MQ=255)
                                                       cGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGATCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGt                                                                                             >  4:1080916/1‑148 (MQ=255)
                                                              cTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACg                                                                                                      <  2:304345/132‑1 (MQ=255)
                                                               ttAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAGATTTCGcc                                                                                                                                             >  2:26341/1‑92 (MQ=255)
                                                                tAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGAt                                                                                    >  3:845070/1‑148 (MQ=255)
                                                                           gAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTa                                                                                                                                     >  3:472180/1‑88 (MQ=255)
                                                                             aGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCg                                                                       <  3:717481/148‑1 (MQ=255)
                                                                                 ggATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGa                                                                                                             <  4:190312/106‑1 (MQ=255)
                                                                                 ggATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGa                                                                                                             >  3:190312/1‑106 (MQ=255)
                                                                                   attattGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCACTCGGTAAc                                                                                                                                   <  3:1030174/82‑1 (MQ=255)
                                                                                   attattGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCACTCGGTAAc                                                                                                                                   >  4:1030174/1‑82 (MQ=255)
                                                                                    ttattGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCtt                                                                                                                                                 <  4:1141467/67‑1 (MQ=255)
                                                                                    ttattGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCtt                                                                                                                                                 >  3:1141467/1‑67 (MQ=255)
                                                                                     tattGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACggg                                                                                                    >  3:376295/1‑111 (MQ=255)
                                                                                     tattGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACag                                                                                        <  1:515345/123‑1 (MQ=255)
                                                                                        tGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTg                                                                               <  3:810193/129‑1 (MQ=255)
                                                                                           tCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAGATTTCGCCAg                                                                                                                                           >  4:815142/1‑66 (MQ=38)
                                                                                           tCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGa                                                                                                             >  4:200745/1‑96 (MQ=255)
                                                                                           tCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGa                                                                                                             <  3:200745/96‑1 (MQ=255)
                                                                                           tCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGcc                                                         <  3:1080916/148‑1 (MQ=255)
                                                                                           gcATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAAc                                                                                                                                   <  3:104305/73‑1 (MQ=255)
                                                                                           acATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAAc                                                                                                                                   >  4:104305/2‑74 (MQ=255)
                                                                                             aTGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATa                                                                                   <  4:388433/120‑1 (MQ=255)
                                                                                             aTGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATa                                                                                   >  3:388433/1‑120 (MQ=255)
                                                                                                aTCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGaa                                                                                                            <  3:529920/92‑1 (MQ=255)
                                                                                                aTCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTg                                                           <  3:883275/141‑1 (MQ=255)
                                                                                                aTCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCa                                                        <  1:481422/144‑1 (MQ=255)
                                                                                                aTCGTCTCCTTGCGGTGTGAGATATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGaa                                                                             <  3:531213/123‑1 (MQ=255)
                                                                                                  cGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCtt                                                                                                                                                 <  1:305727/53‑1 (MQ=255)
                                                                                                  cGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCa                                                                                                                <  3:918748/86‑1 (MQ=255)
                                                                                                    tctcCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCgg                                                                                                                                       <  2:512191/61‑1 (MQ=255)
                                                                                                    tctcCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAg                                                                                                >  4:342821/1‑100 (MQ=255)
                                                                                                    tctcCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAg                                                                                                <  3:342821/100‑1 (MQ=255)
                                                                                                      tcCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAg                                                                         <  4:908050/121‑1 (MQ=255)
                                                                                                       ccTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTa                                                                                                                            >  4:86800/1‑69 (MQ=255)
                                                                                                        cTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTtg                                                 <  3:51338/143‑1 (MQ=255)
                                                                                                         ttGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTg                                                           <  2:870070/132‑1 (MQ=255)
                                                                                                          tGCGGTGTGAGGTATAACAAGGGTAGCCGTGACTGGCGAAGATTTCGcc                                                                                                                                             <  4:70379/49‑1 (MQ=25)
                                                                                                              gtgtGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAatgatg                                      <  2:1119757/148‑1 (MQ=255)
                                                                                                                gtgAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAg                                                                         <  3:865312/111‑1 (MQ=255)
                                                                                                                  gAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGt                                                                                 <  4:926538/101‑1 (MQ=255)
                                                                                                                  gAGGTATAACAAGGGTAGACGTGACGGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCAATCACGAATTGACGGGCAAGGGTTa                                                                                           <  2:508978/91‑1 (MQ=255)
                                                                                                                       ataACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGc                                                                                                                     >  2:899088/1‑60 (MQ=255)
                                                                                                                       ataACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGc                                                                                                                     <  1:899088/60‑1 (MQ=255)
                                                                                                                       ataACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGt                                                                                 >  2:733240/1‑96 (MQ=255)
                                                                                                                         aaCAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAg                                                                         <  3:326059/102‑1 (MQ=255)
                                                                                                                         aaCAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAg                                                                         >  4:326059/1‑102 (MQ=255)
                                                                                                                          aCAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGc                                                      <  2:659118/120‑1 (MQ=255)
                                                                                                                            aaGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTa                                                                   <  2:837421/105‑1 (MQ=255)
                                                                                                                             aGGGTAGACGTGACTAGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCg                                                                       >  4:851568/1‑100 (MQ=255)
                                                                                                                               ggTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGcgcagcga                     <  4:476027/148‑8 (MQ=255)
                                                                                                                                 tAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGAt                                                                                    <  4:275271/83‑1 (MQ=255)
                                                                                                                                 tAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGAt                                                                                    >  3:275271/1‑83 (MQ=255)
                                                                                                                                    aCGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGAt                                                                                    >  1:744257/1‑80 (MQ=255)
                                                                                                                                       tGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTAc                                                                                                       >  4:1004723/1‑58 (MQ=255)
                                                                                                                                       tGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTAc                                                                                                       <  3:1004723/58‑1 (MQ=255)
                                                                                                                                         aCTGGCGAAGATTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGt                                                                                 <  2:336737/78‑1 (MQ=38)
                                                                                                                                         aCTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGaa                                                                             >  3:585828/1‑82 (MQ=255)
                                                                                                                                           tGGCGAAGATTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTa                                                                   >  4:234305/1‑90 (MQ=255)
                                                                                                                                           tGGCGAAGATTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTa                                                                   <  3:234305/90‑1 (MQ=255)
                                                                                                                                           tGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCg                                                                       <  2:215588/86‑1 (MQ=255)
                                                                                                                                           tGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGcc                                                     >  1:759331/1‑104 (MQ=255)
                                                                                                                                           tGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGcc                                                     <  2:759331/104‑1 (MQ=255)
                                                                                                                                            ggCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAg                                                                                                >  1:177787/1‑60 (MQ=255)
                                                                                                                                            ggCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAatgat                                       >  4:511665/1‑117 (MQ=255)
                                                                                                                                            ggCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACaa        >  4:603856/1‑148 (MQ=255)
                                                                                                                                                aaaTCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTa                                                                                                        >  4:656674/1‑48 (MQ=255)
                                                                                                                                                aaaTCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTa                                                                                                        <  3:656674/48‑1 (MQ=255)
                                                                                                                                                aaaTCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAggg                                                                                              >  2:444175/1‑58 (MQ=255)
                                                                                                                                                aaaTCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAggg                                                                                              <  1:444175/58‑1 (MQ=255)
                                                                                                                                                aaaTCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATa                                                                                   <  2:580989/69‑1 (MQ=255)
                                                                                                                                                aaaTCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATa                                                                                   >  1:580989/1‑69 (MQ=255)
                                                                                                                                                aaaTCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGtt                                                  <  1:1128244/102‑1 (MQ=255)
                                                                                                                                                aaaTCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGt                                                   >  4:525665/1‑101 (MQ=255)
                                                                                                                                                aaaTCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGt                                                   <  3:525665/101‑1 (MQ=255)
                                                                                                                                                aaaTCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCcaca         >  4:365155/1‑143 (MQ=255)
                                                                                                                                                aaaTCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATttc    >  3:951124/1‑148 (MQ=255)
                                                                                                                                                   aTCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATttctt  >  2:1114640/1‑148 (MQ=255)
                                                                                                                                                    tCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGt                                                                                 <  1:111936/68‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TCGCCGCCAATAAAGTTGTCATAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTT  >  NC_000913/3756417‑3756710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: