Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,774,207 C→T 9.0% intergenic (+13/‑217) mtlA → / → mtlD mannitol‑specific PTS enzyme: IIA, IIB and IIC components/mannitol‑1‑phosphate dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,774,2070CT 9.0% 1060.4 / 70.9 334intergenic (+13/‑217)mtlA/mtlDmannitol‑specific PTS enzyme: IIA, IIB and IIC components/mannitol‑1‑phosphate dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base C (150/154);  new base T (15/15);  total (165/169)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.43e-01

Only 100 of 355 total aligned reads displayed.

CTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCC  >  NC_000913/3774061‑3774347
                                                                                                                                                  |                                                                                                                                            
    ccGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCTTCTcc                                                                                                                                         >  3:429790/1‑148 (MQ=255)
       tAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCcaca                                                                                                                                      <  2:242032/148‑1 (MQ=255)
        aacaacGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACAt                                                                                                                                     <  2:4168/148‑1 (MQ=255)
         acaacGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCtctc                                                                                                                                          <  3:281276/142‑1 (MQ=255)
         acaacGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCtctc                                                                                                                                          >  4:281276/1‑142 (MQ=255)
               aacacaTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCATCATCGAGCGTCTGGCACAGACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAg                                                                                                                              >  4:960438/3‑148 (MQ=255)
               aGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCtctc                                                                                                                                          >  2:1077646/1‑136 (MQ=255)
                 cacaTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGt                                                                                                                            <  3:917294/148‑1 (MQ=255)
                  acaTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTg                                                                                                                           <  1:91553/148‑1 (MQ=255)
                  acaTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTg                                                                                                                           <  1:1004338/148‑1 (MQ=255)
                   caTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCcaca                                                                                                                                      >  3:798349/1‑136 (MQ=255)
                   caTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTgg                                                                                                                          <  2:496144/148‑1 (MQ=255)
                   caTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTgg                                                                                                                          >  3:475507/1‑148 (MQ=255)
                     ttCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTgggg                                                                                                                        >  4:611348/1‑148 (MQ=255)
                      tCAGGTTATCACCGGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCcaca                                                                                                                                      >  3:77237/1‑133 (MQ=255)
                      tCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTCGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGgag                                                                                                                                 >  1:500673/1‑138 (MQ=255)
                         ggTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGgag                                                                                                                                 >  2:780607/1‑135 (MQ=255)
                           ttATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCTTCTCCATGTGGAGAGGGTggga                                                                                                                        >  2:373192/1‑141 (MQ=255)
                           ttATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAAtt                                                                                                                  >  3:441284/1‑148 (MQ=255)
                                 ccAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTgg                                                                                                                          <  1:562911/134‑1 (MQ=255)
                                  cAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAgg                                                                                                                             >  3:572149/1‑130 (MQ=255)
                                  cAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAgg                                                                                                                             <  4:572149/130‑1 (MQ=255)
                                  cAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGAT                                                                                                           >  3:38085‑M1/1‑148 (MQ=255)
                                   aGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCATCCTCTCCACATGGAGAAgg                                                                                                                             >  4:85243/1‑129 (MQ=255)
                                   aGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTg                                                                                                                           >  2:934660/1‑131 (MQ=255)
                                           cAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCcaca                                                                                                                                      <  2:647141/112‑1 (MQ=255)
                                            aaTGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGaa                                                                                                                               >  1:526933/1‑118 (MQ=255)
                                            aaTGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGaa                                                                                                                               <  2:526933/118‑1 (MQ=255)
                                            aaTGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCt                                                                                                 <  3:611348/148‑1 (MQ=255)
                                            aaTGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCt                                                                                                 >  1:657717/1‑148 (MQ=255)
                                                  cTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTgg                                                                                                                          <  1:641905/117‑1 (MQ=255)
                                                  cTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTatcag                                                                                           >  4:134538‑M2/1‑143 (MQ=255)
                                                  cTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTatcag                                                                                           >  4:38085‑M2/1‑143 (MQ=255)
                                                   tGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTatcagg                                                                                          >  2:897892‑M2/1‑142 (MQ=255)
                                                    ggATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTatcaggc                                                                                         <  1:887534‑M2/148‑8 (MQ=255)
                                                    ggATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGC                                                                                                                >  3:131655‑M1/1‑125 (MQ=255)
                                                     gatgatGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGcc                                                                                        >  3:641268/1‑148 (MQ=255)
                                                      atgatgAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAGTCCCACCCACTCCACATGGAGAAGGTGGGGTTaa                                                                                                                    <  1:610322/119‑1 (MQ=255)
                                                      atgatgAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTaa                                                                                                                    >  2:610322/1‑119 (MQ=255)
                                                      atgatgAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTa                                                                                                     <  1:758301/134‑1 (MQ=255)
                                                        gatgaGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTAc                                                                                     >  2:562429/1‑148 (MQ=255)
                                                         atgaGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCTTCTCCATGTGGAGAGGGTgggat                                                                                                                       >  1:433344/1‑111 (MQ=38)
                                                         atgaGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCcaca                                                                                                                                      <  1:970365/98‑1 (MQ=255)
                                                         atgaGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTgg                                                                                                                          <  2:695034/110‑1 (MQ=255)
                                                         atgaGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACa                                                                                    <  4:441284/148‑1 (MQ=255)
                                                          tgaGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGt                                                                                                                            >  4:495769/1‑107 (MQ=255)
                                                            aGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTgg                                                                                                                          <  4:1043096/107‑1 (MQ=255)
                                                            aGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGC                                                                                                                >  4:455295‑M1/1‑117 (MQ=255)
                                                              tCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACAt                                                                                                                                     <  4:1068231/94‑1 (MQ=255)
                                                              tCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACAt                                                                                                                                     >  3:1068231/1‑94 (MQ=255)
                                                              tCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGGGAAGGt                                                                                                                            >  1:79231/1‑103 (MQ=255)
                                                               ccGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAAt                                                                                                                   <  1:1101244/111‑1 (MQ=255)
                                                                 gTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTa                                                                                      >  2:871620/1‑138 (MQ=255)
                                                                 gTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTa                                                                                      <  1:871620/138‑1 (MQ=255)
                                                                   cATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTgg                                                                                                                          <  4:1093979/100‑1 (MQ=255)
                                                                   cATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTaa                                                                                                                    >  1:386802/1‑106 (MQ=255)
                                                                        aGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCTTCTCCATGTGGAGAGGGTggg                                                                                                                         >  2:669362/1‑96 (MQ=38)
                                                                        aGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTa                                                                                                                     <  2:996699/100‑1 (MQ=255)
                                                                        aGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTa                                                                                                                     >  1:996699/1‑100 (MQ=255)
                                                                        aGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAAt                                                                                                                   <  3:495898/102‑1 (MQ=255)
                                                                        aGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTa                                                                                               >  4:360832‑M2/1‑121 (MQ=255)
                                                                        aGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTa                                                                                               <  3:360832‑M2/122‑2 (MQ=255)
                                                                         gCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTatc                                                                                             <  1:360525‑M2/123‑4 (MQ=255)
                                                                             caaattcaacaaattgtgatgcatcctgtaggcctgataagcgtagcgcatcaggcaattAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTg                                                                                                                 <  3:72373‑M2/39‑1 (MQ=255)
                                                                                gCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGaa                                                                                                                               >  2:472071/1‑82 (MQ=255)
                                                                                   cacacCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGt                                                                                                                            <  4:417780/82‑1 (MQ=255)
                                                                                   cacacCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTg                                                          <  4:873329/148‑1 (MQ=255)
                                                                                     caccacCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCt                                                                                                 <  2:105663/107‑1 (MQ=255)
                                                                                     caccacCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCt                                                                                                 >  1:105663/1‑107 (MQ=255)
                                                                                     caccacCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGa                                                                                 >  4:506986/1‑123 (MQ=255)
                                                                                       ccaccaGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTatc                                                                                             >  3:1040463‑M2/1‑106 (MQ=255)
                                                                                       ccaccaGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTG                                                                                                                 <  2:403502‑M1/89‑1 (MQ=255)
                                                                                        caccaGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTggg                                                                                                                         <  1:30302/80‑1 (MQ=255)
                                                                                        caccaGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTggg                                                                                                                         >  2:30302/1‑80 (MQ=255)
                                                                                         accaGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTatc                                                                                             <  3:514069‑M2/107‑4 (MQ=255)
                                                                                            aGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTg                                                                                                                           >  2:440088/1‑74 (MQ=255)
                                                                                                     gAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAAt                                                                      <  1:584507/118‑1 (MQ=255)
                                                                                                     gAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGAGCTACGCTTATCAGGCCTACAGGATGCATCACAAt                                                                      >  2:584507/1‑118 (MQ=255)
                                                                                                        gTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGt                                                                                                                            >  4:643221/1‑61 (MQ=255)
                                                                                                              gAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATaagcgtagcgcatcagg                                                                                          >  3:702307‑M1/1‑72 (MQ=255)
                                                                                                               aaCTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCtt                                                 <  4:713295/129‑1 (MQ=255)
                                                                                                                   gctgGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTAcg                          <  2:758561/148‑1 (MQ=255)
                                                                                                                     tgGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAAt                                                                      >  1:457872/1‑102 (MQ=255)
                                                                                                                      ggCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAAtt                                                            <  3:87221/111‑1 (MQ=255)
                                                                                                                        cAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATaa                                    <  4:1021264/133‑1 (MQ=255)
                                                                                                                        cAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATaa                                    >  3:1021264/1‑133 (MQ=255)
                                                                                                                            tCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTg                                                          >  3:1087352/1‑107 (MQ=255)
                                                                                                                             cGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAgg                                                                                  >  3:983751/1‑82 (MQ=255)
                                                                                                                                       ccaatccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCt                                                                                                 <  3:537946/57‑1 (MQ=255)
                                                                                                                                       ccaatccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCt                                                                                                 >  4:537946/1‑57 (MQ=255)
                                                                                                                                       ccaatccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCt                                                  >  2:885655/1‑104 (MQ=255)
                                                                                                                                         aatccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGa                                                               >  1:242430/1‑89 (MQ=255)
                                                                                                                                          atccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCtt                                                 >  4:781542/1‑102 (MQ=255)
                                                                                                                                              cACCTTCTCCATGTGGAGAGGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAg                                   >  4:727105/1‑112 (MQ=37)
                                                                                                                                               aCCTTCTCCATGTGGAGAGGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATttgt                                                                  >  1:683569/1‑80 (MQ=25)
                                                                                                                                               aCCTTCTCCATGTGGAGAGGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGtt                                                    <  4:255667/94‑1 (MQ=25)
                                                                                                                                               aCCTTCTCCATGTGGAGAGGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGtt                                                    >  3:255667/1‑94 (MQ=37)
                                                                                                                                               aCCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGtt                                                    >  1:414923/1‑94 (MQ=255)
                                                                                                                                                  ctctCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCtt                                                 >  1:174425/1‑94 (MQ=255)
                                                                                                                                                  ctctCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCtt                                                 <  2:174425/94‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
CTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCC  >  NC_000913/3774061‑3774347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: