Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,774,222 A→G 8.4% intergenic (+28/‑202) mtlA → / → mtlD mannitol‑specific PTS enzyme: IIA, IIB and IIC components/mannitol‑1‑phosphate dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,774,2220AG 8.4% 1012.1 / 58.2 309intergenic (+28/‑202)mtlA/mtlDmannitol‑specific PTS enzyme: IIA, IIB and IIC components/mannitol‑1‑phosphate dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base A (140/143);  new base G (12/14);  total (152/157)
Fisher's exact test for biased strand distribution p-value = 8.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-01

Only 100 of 315 total aligned reads displayed.

AGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCC  >  NC_000913/3774076‑3774347
                                                                                                                                                  |                                                                                                                             
aacacaTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCATCATCGAGCGTCTGGCACAGACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAg                                                                                                                              >  4:960438/3‑148 (MQ=255)
aGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAg                                                                                                                              >  1:997717/1‑148 (MQ=255)
  cacaTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGt                                                                                                                            >  2:652577/1‑148 (MQ=255)
  cacaTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGt                                                                                                                            >  1:897892/1‑148 (MQ=255)
  cacaTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGt                                                                                                                            <  1:652577/148‑1 (MQ=255)
   acaTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTg                                                                                                                           <  1:1004338/148‑1 (MQ=255)
    caTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTgg                                                                                                                          <  2:496144/148‑1 (MQ=255)
    caTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTgg                                                                                                                          >  1:581522/1‑148 (MQ=255)
      ttCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTgggg                                                                                                                        >  4:611348/1‑148 (MQ=255)
       tCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAgg                                                                                                                             <  1:987444/142‑1 (MQ=255)
           gTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGCCGTAAGTAATCCAACCCCACCTTCTCCATGTGGAGAGGGTGGGAttgg                                                                                                                    >  2:672174/1‑145 (MQ=38)
            ttATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCTTCTCCATGTGGAGAGGGTggga                                                                                                                        >  2:373192/1‑141 (MQ=255)
               tCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCC                                                                                                               <  3:374878‑M1/148‑1 (MQ=255)
                  ccAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGA                                                                                                            >  2:1068834‑M1/1‑148 (MQ=255)
                   cAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAgg                                                                                                                             >  3:572149/1‑130 (MQ=255)
                    aGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCATCCTCTCCACATGGAGAAgg                                                                                                                             >  4:85243/1‑129 (MQ=255)
                         gACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTa                                                                                                     <  1:1068834/148‑1 (MQ=255)
                                   cTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTatcag                                                                                           >  4:38085‑M2/1‑143 (MQ=255)
                                     ggATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTg                                                                                                                           >  3:720915/1‑114 (MQ=255)
                                     ggATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTatca                                                                                            >  4:914439‑M2/1‑141 (MQ=255)
                                     ggATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGC                                                                                                                >  3:131655‑M1/1‑125 (MQ=255)
                                      gatgatGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGcc                                                                                        >  3:641268/1‑148 (MQ=255)
                                       atgatgAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTaa                                                                                                                    <  1:478348/119‑1 (MQ=255)
                                       atgatgAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAAt                                                                                                                   <  1:542499/120‑1 (MQ=255)
                                       atgatgAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTa                                                                                                     <  1:758301/134‑1 (MQ=255)
                                         gatgaGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTAc                                                                                     >  2:562429/1‑148 (MQ=255)
                                         gatgaGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCC                                                                                                               <  4:1115367‑M1/122‑1 (MQ=255)
                                          atgaGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCTTCTCCATGTGGAGAGGGTgggat                                                                                                                       >  1:433344/1‑111 (MQ=38)
                                          atgaGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGaa                                                                                                                               <  1:116603/105‑1 (MQ=255)
                                          atgaGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGaa                                                                                                                               >  2:116603/1‑105 (MQ=255)
                                          atgaGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTgg                                                                                                                          >  1:695034/1‑110 (MQ=255)
                                           tgaGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGt                                                                                                                            >  4:495769/1‑107 (MQ=255)
                                           tgaGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGt                                                                                                                            <  3:495769/107‑1 (MQ=255)
                                             aGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTatcagg                                                                                          <  3:1036438‑M2/139‑7 (MQ=255)
                                             aGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGC                                                                                                                >  1:454737‑M1/1‑117 (MQ=255)
                                             aGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGC                                                                                                                >  4:455295‑M1/1‑117 (MQ=255)
                                             aGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGC                                                                                                                <  3:455295‑M1/117‑1 (MQ=255)
                                              gTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCt                                                                                                 <  4:1062572/131‑1 (MQ=255)
                                               tCCGTCATCGAGCGTCTGGCACACACCGCCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGt                                                                                                                            <  2:79231/103‑1 (MQ=255)
                                               tCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTgg                                                                                                                          >  1:934200/1‑105 (MQ=255)
                                               tCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTgg                                                                                                                          <  2:934200/105‑1 (MQ=255)
                                               tCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATg                                                                               >  3:873329/1‑148 (MQ=255)
                                                ccGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAAt                                                                                                                   >  1:219327/1‑111 (MQ=255)
                                                ccGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGc                                                                              >  3:1050813/1‑148 (MQ=255)
                                                  gTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTG                                                                                                             >  1:411239‑M1/1‑115 (MQ=255)
                                                    cATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTgg                                                                                                                          >  3:1093979/1‑100 (MQ=255)
                                                       cGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACaa                                                                       >  1:350012/1‑148 (MQ=255)
                                                         aGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCTTCTCCATGTGGAGAGGGTggg                                                                                                                         <  1:669362/96‑1 (MQ=38)
                                                         aGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTa                                                                                                                     <  2:996699/100‑1 (MQ=255)
                                                           cGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTgggg                                                                                                                        >  3:54471/1‑95 (MQ=255)
                                                             tCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATttgtt                                                                 >  3:1007202/1‑148 (MQ=255)
                                                              caaattcaacaaattgtgatgcatcctgtaggcctgataagcgtagcgcatcaggcaattAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTg                                                                                                                 >  4:72373‑M2/61‑99 (MQ=255)
                                                                ggCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATgcg                                                                                                        <  3:409390/106‑1 (MQ=255)
                                                                ggCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATg                                                                               <  4:548026/131‑1 (MQ=255)
                                                                    cacacCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGt                                                                                                                            <  4:417780/82‑1 (MQ=255)
                                                                    cacacCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTaa                                                                                                                    >  4:586219/1‑90 (MQ=255)
                                                                    cacacCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACa                                                                                    <  2:895945/122‑1 (MQ=255)
                                                                      caccacCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGa                                                                                 >  4:506986/1‑123 (MQ=255)
                                                                      caccacCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGa                                                                                 <  3:506986/123‑1 (MQ=255)
                                                                        ccaccaGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTatc                                                                                             <  4:1040463‑M2/109‑4 (MQ=255)
                                                                        ccaccaGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTG                                                                                                                 <  2:403502‑M1/89‑1 (MQ=255)
                                                                         caccaGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTggg                                                                                                                         <  1:30302/80‑1 (MQ=255)
                                                                          accaGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTggg                                                                                                                         >  4:527207/1‑79 (MQ=255)
                                                                           ccaGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCTTCTCCATGTGGAGAGGGTggg                                                                                                                         >  1:588839/1‑78 (MQ=37)
                                                                           ccaGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTatcagg                                                                                          <  2:583463‑M2/109‑7 (MQ=255)
                                                                           ccaGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGC                                                                                                                >  3:522140‑M1/1‑87 (MQ=255)
                                                                             aGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTg                                                                                                                           >  2:440088/1‑74 (MQ=255)
                                                                               cGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTatca                                                                                            <  4:440400‑M2/103‑5 (MQ=255)
                                                                                              ggAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGC                                                                                                                >  2:729934‑M1/1‑68 (MQ=255)
                                                                                               gAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATaagcgtagcgcatcagg                                                                                          <  4:702307‑M1/89‑18 (MQ=255)
                                                                                               gAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATaagcgtagcgcatcagg                                                                                          >  3:702307‑M1/1‑72 (MQ=255)
                                                                                                tgataagcgtagcgcatcaggcaattAATCCCACCCTCTCCACATGGAGAAGGTgg                                                                                                                          >  3:1069646‑M2/27‑56 (MQ=255)
                                                                                                aaCTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTatca                                                                                            >  1:378604‑M2/1‑82 (MQ=255)
                                                                                                    gctgGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGc                                                                              >  4:34323/1‑96 (MQ=255)
                                                                                                    gctgGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGc                                                                              <  3:34323/96‑1 (MQ=255)
                                                                                                    gctgGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTAcg                          <  2:758561/148‑1 (MQ=255)
                                                                                                      tgGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAAGCCTACAGGATGCATCACAAt                                                                      <  2:457872/102‑1 (MQ=255)
                                                                                                         cAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATaa                                    <  4:1021264/133‑1 (MQ=255)
                                                                                                              cGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAgg                                                                                  >  3:983751/1‑82 (MQ=255)
                                                                                                                  agtaaTCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGcc                                          >  4:108342/1‑118 (MQ=255)
                                                                                                                     aatccaatccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATttgt                                                                  <  3:600172/91‑1 (MQ=255)
                                                                                                                        ccaatccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCt                                                  >  2:885655/1‑104 (MQ=255)
                                                                                                                        ccaatccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCt                                                  <  1:885655/104‑1 (MQ=255)
                                                                                                                          aatccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGa                                                               <  2:242430/89‑1 (MQ=255)
                                                                                                                           atccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGtt                                                    >  4:147516/1‑99 (MQ=255)
                                                                                                                           atccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCtt                                                 >  4:781542/1‑102 (MQ=255)
                                                                                                                               cACCTTCTCCATGTGGAGAGGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAAttt                                                           >  2:589804/1‑88 (MQ=25)
                                                                                                                               cACCTTCTCCATGTGGAGAGGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAg                                   <  3:727105/112‑1 (MQ=25)
                                                                                                                                accttctcCATGTGGAGAGGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTctcc  <  2:613325/140‑1 (MQ=255)
                                                                                                                                aCCTTCTCCATGTGGAGAGGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGtt                                                    <  4:255667/94‑1 (MQ=25)
                                                                                                                                aCCTTCTCCATGTGGAGAGGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGtt                                                    >  3:255667/1‑94 (MQ=37)
                                                                                                                                aCCTTCTCCATGTGGAGAGGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTctcc  >  1:613325/1‑144 (MQ=255)
                                                                                                                                aCCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGtt                                                    <  2:414923/94‑1 (MQ=255)
                                                                                                                                   ctctCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCtt                                                 >  3:431660/1‑94 (MQ=255)
                                                                                                                                     ctcCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGa                                                               <  4:271069/78‑1 (MQ=255)
                                                                                                                                     ctcCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGa                                                               >  3:271069/1‑78 (MQ=255)
                                                                                                                                      tcCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCt                                                                                       >  3:826580/1‑53 (MQ=255)
                                                                                                                                       ccACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATaa                                    <  1:785381/103‑1 (MQ=255)
                                                                                                                                         acaTGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAGTTTGCACGTTCTTGTAGGCCGGATAAg                                   <  2:313074/102‑1 (MQ=255)
                                                                                                                                               agaAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACaa                                                                       >  3:918562/1‑60 (MQ=255)
                                                                                                                                                  |                                                                                                                             
AGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCC  >  NC_000913/3774076‑3774347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: