Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,776,127 A→G 7.5% K186E (AAA→GAA)  mtlR → mannitol operon repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,776,1270AG 7.5% 846.3 / 38.4 253K186E (AAA→GAA) mtlRmannitol operon repressor
Reads supporting (aligned to +/- strand):  ref base A (118/115);  new base G (9/10);  total (127/126)
Fisher's exact test for biased strand distribution p-value = 8.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 263 total aligned reads displayed.

CCACCGCCGCCACAGTTTGAACCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTCCGGACGGAATCGCG  >  NC_000913/3776013‑3776273
                                                                                                                  |                                                                                                                                                   
ccaccGCCGCCACAGTTTGAACCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCaa                                                                                                                                                    >  1:612449/1‑116 (MQ=255)
  accGCCGCCACAGTTTGAACCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTTGGAAATCAg                                                                                                                                              >  4:817518/1‑120 (MQ=255)
  accGCCGCCACAGTTTGAACCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTTGGAAATCAGCtc                                                                                                                                           >  2:475639/1‑122 (MQ=255)
  accGCCGCCACAGTTTGAACCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCa                                                                                                                                               <  1:604712/119‑1 (MQ=255)
  accGCCGCCACAGTTTGAACCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAg                                                                                                                                              >  3:263640/1‑120 (MQ=255)
  accGCCGCCACAGTTTGAACCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAg                                                                                                                                              <  4:263640/120‑1 (MQ=255)
  accGCCGCCACAGTTTGAACCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTaaa                                                                                                                                        >  3:671067/1‑126 (MQ=255)
  accGCCGCCACAGTTTGAACCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTaaa                                                                                                                                        <  4:671067/126‑1 (MQ=255)
  accGCCGCCACAGTTTGAACCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTAAGCTGATTTTGGAAATCAg                                                                                                                                              >  4:906732/1‑120 (MQ=255)
     gccgccACAGTTTGAACCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACg                                                                                                              <  3:570984/148‑1 (MQ=255)
      ccgccACAGTTTGAACCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGt                                                                                                             >  4:545786/1‑148 (MQ=255)
        gccACAGTTTGAACCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCttt                                                                                                                              <  2:609025/130‑1 (MQ=255)
          cacaGTTTGAACCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCaaa                                                                                                                          >  1:804717/1‑132 (MQ=255)
            caGTTTGAACCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATc                                                                                                                                                >  4:1136096/1‑108 (MQ=255)
            caGTTTGAACCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATc                                                                                                                                                <  3:1136096/108‑1 (MQ=255)
            caGTTTGAACCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCaaa                                                                                                                          >  3:44983/1‑130 (MQ=255)
            caGTTTGAACCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTa                                                                                                                      <  2:496316/134‑1 (MQ=255)
                  gAACCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGcc                                                                                                 <  3:184110/148‑1 (MQ=255)
                   aaCCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAATTCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTg                                                                                                          <  1:68175/138‑1 (MQ=255)
                    aCCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTaaaaaa                                                                                                                                     <  3:346347/111‑1 (MQ=255)
                       agcagACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCtt                                                                                                                               >  2:207897/1‑114 (MQ=255)
                            aCTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTg                                                                                       <  1:245269/148‑1 (MQ=255)
                               ccAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTaaaaaa                                                                                                                                     <  1:494279/100‑1 (MQ=255)
                               ccAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCtt                                                                                                                               <  3:486465/106‑1 (MQ=255)
                                 aGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAg                                                                                                                                              <  3:919623/89‑1 (MQ=255)
                                  gTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTTGGAAATCAg                                                                                                                                              <  2:671117/88‑1 (MQ=255)
                                  gTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCgg                                                                                 >  2:984518/1‑148 (MQ=255)
                                   tttGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTa                                                                                                                      >  2:130841/1‑111 (MQ=255)
                                    ttGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGcc                                                                                                 <  1:153139/130‑1 (MQ=255)
                                        atgcaatgcaAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGATCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCaaa                                                                                                                          <  2:283351/102‑1 (MQ=255)
                                        atgcaatgcaAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTTGGAAATCAGCt                                                                                                                                            <  4:329849/84‑1 (MQ=255)
                                        atgcaatgcaAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTTGGAAATCAGCt                                                                                                                                            >  3:329849/1‑84 (MQ=255)
                                        atgcaatgcaAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCaaa                                                                                                                          >  1:655646/1‑102 (MQ=255)
                                          gcaatgcaaATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATaaa                                                                         >  3:818729/1‑148 (MQ=255)
                                          gcaatgcaaATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATaaa                                                                         >  1:846750/1‑148 (MQ=255)
                                           caatgcaaATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCaaa                                                                                                                          <  1:120589/99‑1 (MQ=255)
                                            aatgcaaATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCa                                                                                                                                                     <  4:190606/71‑1 (MQ=255)
                                            aatgcaaATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCg                                                                              >  2:648875/1‑141 (MQ=255)
                                             atgcaaATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCtt                                                                                                                               >  3:847978/1‑92 (MQ=255)
                                              tgcaaATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTTGGAAATCAGCtc                                                                                                                                           <  3:1027037/79‑2 (MQ=38)
                                              tgcaaATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTaaaaaa                                                                                                                                     >  1:1026399/1‑85 (MQ=255)
                                              tgcaaATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGccc                                                                                                <  4:401361/121‑1 (MQ=255)
                                               gcaaATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTg                                                                                                   >  1:516086/1‑117 (MQ=255)
                                                caaATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAGACGTTGCTTAc                                                                                                     >  2:697638/1‑114 (MQ=255)
                                                caaATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTc                                                                                                            >  2:856436/1‑107 (MQ=255)
                                                caaATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCt                                                                                     <  4:1077564/130‑1 (MQ=255)
                                                 aaaTTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCcgacg                                                                  <  4:830063/148‑1 (MQ=255)
                                                  aaTTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCct                                                                                              >  4:1045550/1‑119 (MQ=255)
                                                    ttCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTACACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCgg                                                                                 >  4:955608/1‑130 (MQ=255)
                                                      cAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGataat                                                             <  1:1093902/148‑1 (MQ=255)
                                                            tATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCa                                                       <  1:984518/148‑1 (MQ=255)
                                                             aTCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCtt                                                                                                                               <  4:1025919/76‑1 (MQ=255)
                                                             aTCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCaaa                                                                                                                          <  3:1114835/81‑1 (MQ=255)
                                                             aTCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAg                                                      <  2:846750/148‑1 (MQ=255)
                                                              tCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTg                                                                                       <  2:322298/114‑1 (MQ=255)
                                                              tCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTc                                                         <  1:1017075/144‑1 (MQ=255)
                                                              tCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGa                                                     <  3:1053966/148‑1 (MQ=255)
                                                               cAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGc                                                                                      <  2:37101/114‑1 (MQ=255)
                                                                 aCAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCg                                                                              >  1:739900/1‑120 (MQ=255)
                                                                 aCAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGt                                                  >  2:9176/1‑148 (MQ=255)
                                                                  cAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCttt                                                                                                                              >  2:794604/1‑72 (MQ=255)
                                                                  cAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCctc                                                                                             <  4:142489/104‑1 (MQ=255)
                                                                  cAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCctc                                                                                             >  3:142489/1‑104 (MQ=255)
                                                                   aGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGa                                                                             >  2:1078478/1‑119 (MQ=255)
                                                                      cTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGc                                                                                                                   <  1:433303/79‑1 (MQ=255)
                                                                       tgtgCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAg                                                                                                                    <  2:1087967/77‑1 (MQ=255)
                                                                           cGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTAc                                                                                          >  1:314065/1‑98 (MQ=255)
                                                                            gATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGc                                                                                <  3:969791/107‑1 (MQ=255)
                                                                              tCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACg                                     >  3:732284/1‑148 (MQ=255)
                                                                                 acaaTGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCt                                                                                                                                >  4:896812/1‑55 (MQ=255)
                                                                                 acaaTGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCt                                                                                                                                <  3:896812/55‑1 (MQ=255)
                                                                                 acaaTGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTg                                                                                       <  1:338339/95‑1 (MQ=255)
                                                                                 acaaTGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCtt                                                                                    <  1:567617/98‑1 (MQ=255)
                                                                                 acaaTGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCCTAAAAAAAGCCttt                                                                                                                              >  2:119920/1‑57 (MQ=255)
                                                                                  caaTGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTg                                                                                                          <  3:799518/75‑1 (MQ=255)
                                                                                  caaTGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTg                                                                                                          >  4:799518/1‑75 (MQ=255)
                                                                                  caaTGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGt                                               <  1:881299/134‑1 (MQ=255)
                                                                                  caaTGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGt                                               >  4:337635/1‑134 (MQ=255)
                                                                                   aaTGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACg                                <  1:9176/148‑1 (MQ=255)
                                                                                     tGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTAc                                                                                                     <  2:587481/77‑1 (MQ=255)
                                                                                        tCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTg                                                                                                          >  2:558896/1‑69 (MQ=255)
                                                                                        tCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGc                                                                                      >  4:501866/1‑89 (MQ=255)
                                                                                        tCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGc                                                                                      <  3:501866/89‑1 (MQ=255)
                                                                                            ttCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAg                                                                                                                    >  4:485060/1‑56 (MQ=255)
                                                                                            ttCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCcg                                                                     <  3:667032/102‑1 (MQ=255)
                                                                                            ttCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCg                       <  1:264295/148‑1 (MQ=255)
                                                                                             tCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCcg                                                                     >  2:510756/1‑101 (MQ=255)
                                                                                                ctgactgaGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATc                        >  4:203779/1‑143 (MQ=255)
                                                                                                ctgactgaGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATc                        <  3:203779/143‑1 (MQ=255)
                                                                                                  gactgaGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCgg                                                                                 >  2:432286/1‑84 (MQ=255)
                                                                                                         cTGATTTTGGAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATaa                                       <  4:225119/119‑1 (MQ=255)
                                                                                                         cTGATTTTGGAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATaa                                       >  3:225119/1‑119 (MQ=255)
                                                                                                          tGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCtt                                                                                    <  2:769134/73‑1 (MQ=255)
                                                                                                              ttCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGAtata                                        >  1:507155/1‑113 (MQ=255)
                                                                                                               tCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTCCGGACGGAATcg    <  4:732284/148‑1 (MQ=255)
                                                                                                                ccAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAg                                                      >  1:777539/1‑97 (MQ=255)
                                                                                                                 cAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACt                                                          <  4:656919/92‑1 (MQ=255)
                                                                                                                 cAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCg                       <  1:108554/127‑1 (MQ=255)
                                                                                                                 cAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTCCGGACGGAATcgcg  <  1:404028/148‑1 (MQ=255)
                                                                                                                  aaaaTCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGa                                            <  2:262826/105‑1 (MQ=255)
                                                                                                                  |                                                                                                                                                   
CCACCGCCGCCACAGTTTGAACCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTCCGGACGGAATCGCG  >  NC_000913/3776013‑3776273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: