Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,790,303 A→T 10.0% intergenic (‑222/+17) waaH ← / ← tdh LPS(HepIII)‑glucuronic acid glycosyltransferase/L‑threonine 3‑dehydrogenase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,790,3030AT10.0% 398.9 / 27.9 119intergenic (‑222/+17)waaH/tdhLPS(HepIII)‑glucuronic acid glycosyltransferase/L‑threonine 3‑dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (54/53);  new base T (6/6);  total (60/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.08e-01

Only 100 of 119 total aligned reads displayed.

GAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCC  >  NC_000913/3790176‑3790449
                                                                                                                               |                                                                                                                                                  
gagTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGtt                                                                                                                                        >  4:947901/1‑140 (MQ=255)
gagTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGtt                                                                                                                                        <  3:947901/140‑1 (MQ=255)
    cACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTg                                                                                                                                          >  3:153591/1‑134 (MQ=255)
              tAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGAGCTGGAATAcc                                                                                                                                                  >  3:559129/1‑116 (MQ=255)
              tAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGAGCTGGAATAcc                                                                                                                                                  <  4:559129/116‑1 (MQ=255)
                         tGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGTATTCCAGccc                                                                                                                                             <  3:590349/110‑1 (MQ=255)
                         tGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGTATTCCAGccc                                                                                                                                             >  4:590349/1‑110 (MQ=255)
                         tGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTc                                                                                                                                       >  1:441745/1‑116 (MQ=255)
                                      ataAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGcc                                                                                                                                              >  1:237806/1‑96 (MQ=255)
                                           agaTCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGaa                                                                                            <  1:842351/141‑1 (MQ=255)
                                                   acaATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGc                                                                                         >  2:515602/1‑136 (MQ=255)
                                                    caATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTaa                                                                                                                                >  4:200363/1‑96 (MQ=255)
                                                      aTTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGTATTCCAGccc                                                                                                                                             <  1:852292/81‑1 (MQ=255)
                                                      aTTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGTATTCCAGccc                                                                                                                                             >  2:852292/1‑81 (MQ=255)
                                                          cGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATAcc                                                                                                                                                  <  4:834670/72‑1 (MQ=255)
                                                          cGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATAcc                                                                                                                                                  >  3:834670/1‑72 (MQ=255)
                                                             aCTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGc                                                                                                 >  2:334231/1‑118 (MQ=255)
                                                             aCTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGc                                                                                                 <  1:334231/118‑1 (MQ=255)
                                                                  ttattaCACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCaa                                                                               >  2:386828/1‑131 (MQ=255)
                                                                  ttattaCACTGACAGGATTTTACATATTTAACAGATTATCCTAAGATAAAAGGGCTGGTATTCCAGCCCt                                                                                                                                            <  3:978766/70‑1 (MQ=38)
                                                                      taCACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCt                                                                                                                        <  1:879092/86‑1 (MQ=255)
                                                                      taCACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCt                                                                                                                        >  2:879092/1‑86 (MQ=255)
                                                                      taCACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAg                                                                                     <  2:423762/121‑1 (MQ=255)
                                                                      taCACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAg                                                                                     >  1:423762/1‑121 (MQ=255)
                                                                       acacTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGtt                                                                                                                                        >  3:908750/1‑69 (MQ=255)
                                                                       acacTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGtt                                                                                                                                        <  4:908750/69‑1 (MQ=255)
                                                                       acacAGACAGGATTTTACATATTTAACAGATTCTCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGAt                                                         >  1:320829/1‑148 (MQ=255)
                                                                         acTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAg                                                                                                                                                >  3:353556/1‑59 (MQ=255)
                                                                         acTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAg                                                                                                                                                <  4:353556/59‑1 (MQ=255)
                                                                              cAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCgt                                                                                                                                     >  4:870863/1‑65 (MQ=255)
                                                                                ggATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATc                                                                                                                              >  2:776477/1‑70 (MQ=255)
                                                                                ggATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGa                                                                                                                    >  2:715106/1‑80 (MQ=255)
                                                                                ggATTTTACAGATTTAACGGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATc                                                                                                                              <  1:776477/70‑1 (MQ=255)
                                                                                  aTTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGcc                                                                                                >  2:904445/1‑98 (MQ=255)
                                                                                   ttttACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTaa                                                                                                                                >  4:514549/1‑65 (MQ=255)
                                                                                   ttttACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTaa                                                                                                                                <  3:514549/65‑1 (MQ=255)
                                                                                          tatTTAACAGATTATCCTCAGATAAAAGGGCTGGAATAcc                                                                                                                                                  <  2:486416/40‑1 (MQ=255)
                                                                                          tatTTAACAGATTATCCTCAGATAAAAGGGCTGGAATAcc                                                                                                                                                  >  1:486416/1‑40 (MQ=255)
                                                                                           atTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGcc                                                                                                >  3:121354/1‑89 (MQ=255)
                                                                                           atTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGcc                                                                                                <  4:121354/89‑1 (MQ=255)
                                                                                             ttAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGAt                                              >  3:1089557/1‑137 (MQ=255)
                                                                                             ttAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGAt                                              <  4:1089557/137‑1 (MQ=255)
                                                                                             ttAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATc                                   <  2:320829/148‑1 (MQ=255)
                                                                                                aCAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGa                                                                                                                    <  2:1010109/64‑1 (MQ=255)
                                                                                                aCAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGa                                                                                                                    >  1:1010109/1‑64 (MQ=255)
                                                                                                aCAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGaa                                                                                            <  3:962087/88‑1 (MQ=255)
                                                                                                aCAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGaa                                                                                            >  4:962087/1‑88 (MQ=255)
                                                                                                aCAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAatcat                                                             <  3:1090954/119‑1 (MQ=255)
                                                                                                aCAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAatcat                                                             >  4:1090954/1‑119 (MQ=255)
                                                                                                     ttATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCg                                                           <  1:411563/116‑1 (MQ=255)
                                                                                                     ttATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCg                                                           >  2:411563/1‑116 (MQ=255)
                                                                                                      tATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGa                          >  2:908731/1‑148 (MQ=255)
                                                                                                      tATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGa                          >  4:767949/1‑148 (MQ=255)
                                                                                                          cTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGa                      <  1:908731/148‑1 (MQ=255)
                                                                                                          cTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGa                      <  3:767949/148‑1 (MQ=255)
                                                                                                           tCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCgtg                                                                                                                                    >  3:812885/1‑37 (MQ=255)
                                                                                                           tCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCgtg                                                                                                                                    <  4:812885/37‑1 (MQ=255)
                                                                                                            cAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTcc                                                                                                          <  4:758604/62‑1 (MQ=255)
                                                                                                            cAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTcc                                                                                                          >  3:758604/1‑62 (MQ=255)
                                                                                                            cAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGaa                                                                                            <  4:724838/76‑1 (MQ=255)
                                                                                                            cAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGa                                                                                             >  4:1061168/1‑75 (MQ=255)
                                                                                                            cAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGc                                                                                    >  3:435786/1‑84 (MQ=255)
                                                                                                            cAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGc                                                                                    <  4:435786/84‑1 (MQ=255)
                                                                                                            cAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAatca                                                              >  1:1064462/1‑106 (MQ=255)
                                                                                                            cAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAatca                                                              <  2:1064462/106‑1 (MQ=255)
                                                                                                            cAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGAt                                              >  4:315560/1‑122 (MQ=255)
                                                                                                            cAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGAt                                              <  3:315560/122‑1 (MQ=255)
                                                                                                            cAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTa                                         <  1:633825/127‑1 (MQ=255)
                                                                                                            cAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTa                                         >  2:633825/1‑127 (MQ=255)
                                                                                                               aTAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATa                                                                                                                 >  2:487310/1‑52 (MQ=255)
                                                                                                               aTAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATa                                                                                                                 <  1:487310/52‑1 (MQ=255)
                                                                                                                tAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACg                                                                                          >  1:680649/1‑74 (MQ=255)
                                                                                                                tAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACg                                                                                          <  2:680649/74‑1 (MQ=255)
                                                                                                                tAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGaa                                                                  <  4:502392/98‑1 (MQ=255)
                                                                                                                tAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGaa                                                                  >  3:502392/1‑98 (MQ=255)
                                                                                                                 aaaaGGGCTGGAATACCAGCCCTTGTTCGCGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTc                                                                       >  1:550685/1‑92 (MQ=255)
                                                                                                                 aaaaGGGCTGGAATACCAGCCCTTGTTCGCGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTc                                                                       <  2:550685/92‑1 (MQ=255)
                                                                                                                  aaaGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGa                                                                                             <  3:361250/69‑1 (MQ=255)
                                                                                                                  aaaGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGa                                                                                             >  4:361250/1‑69 (MQ=255)
                                                                                                                    aGGGCTGGTATTCCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTaa                                        >  1:637083/1‑120 (MQ=255)
                                                                                                                    aGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAatcat                                                             <  3:676851/99‑1 (MQ=255)
                                                                                                                     gggCTGGTATTCCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGc                                                                                         <  3:12593/70‑1 (MQ=39)
                                                                                                                     gggCTGGTATTCCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGc                                                                                         >  4:12593/1‑70 (MQ=39)
                                                                                                                     gggCTGGTATTCCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTaa                                        <  3:270705/119‑1 (MQ=255)
                                                                                                                     gggCTGGTATTCCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTaa                                        >  4:270705/1‑119 (MQ=255)
                                                                                                                       gCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACg                                                >  1:920896/1‑109 (MQ=255)
                                                                                                                       gCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACg                                                <  2:920896/109‑1 (MQ=255)
                                                                                                                        cTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACg                                                <  1:782384/108‑1 (MQ=255)
                                                                                                                        cTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACg                                                >  2:782384/1‑108 (MQ=255)
                                                                                                                        cTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGaa                             >  1:667857/1‑127 (MQ=255)
                                                                                                                        cTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGaa                             <  2:667857/127‑1 (MQ=255)
                                                                                                                          ggAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATg                                             <  1:772078/109‑1 (MQ=255)
                                                                                                                          ggAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATg                                             >  2:772078/1‑109 (MQ=255)
                                                                                                                            aaTACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCtt                                                                        <  2:1053143/80‑1 (MQ=255)
                                                                                                                            aaTACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCtt                                                                        >  1:1053143/1‑80 (MQ=255)
                                                                                                                            aaTACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGa                              >  3:838886/1‑122 (MQ=255)
                                                                                                                             aTACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATTGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGAt                                    >  4:661222/1‑115 (MQ=255)
                                                                                                                             aTACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGAt                                    <  3:661222/115‑1 (MQ=255)
                                                                                                                              tACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGaa                             <  3:884275/121‑1 (MQ=255)
                                                                                                                              tACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGaa                             >  4:884275/1‑121 (MQ=255)
                                                                                                                               |                                                                                                                                                  
GAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCC  >  NC_000913/3790176‑3790449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: