Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 339,764 G→C 14.9% intergenic (+21/‑401) yahG → / → yahI DUF1116 family protein/carbamate kinase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913339,7640GC14.9% 134.8 / 15.9 47intergenic (+21/‑401)yahG/yahIDUF1116 family protein/carbamate kinase‑like protein
Reads supporting (aligned to +/- strand):  ref base G (20/20);  new base C (3/4);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

CACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGGCGCGGGCATTGTGCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGG  >  NC_000913/339626‑339878
                                                                                                                                          |                                                                                                                  
cacCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGGCGCGGGCATTGTGCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGaa                                                                                                           <  1:531834/148‑1 (MQ=255)
 acCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGGCGCGGGCATTGTGCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCACGCCGCATCCGGCATGAAc                                                                                                          <  4:205750/148‑1 (MQ=255)
             cATAAAGATGCGGGCGTCGGCATGATTGGCGCGGGCATTGTGCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCACGCCGCATCCGGCATg                                                                                                             >  2:657692/1‑133 (MQ=255)
             cATAAAGATGCGGGCGTCGGCATGATTGGCGCGGGCATTGTGCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCACGCCGCATCCGGCATg                                                                                                             <  1:657692/133‑1 (MQ=255)
             cATAAAGATGCGGGCGTCGGCATGATTGGCGCGGGCATTGTGCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCACGCCGCATCCGGCATGaa                                                                                                           <  1:267484/135‑1 (MQ=255)
             cATAAAGATGCGGGCGTCGGCATGATTGGCGCGGGCATTGTGCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCACGCCGCATCCGGCATGaa                                                                                                           >  2:267484/1‑135 (MQ=255)
               tAAAGATGCGGGCGTCGGCATGATTGGCGCGGGCATTGTGCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTa                                                                                                    <  1:384288/140‑1 (MQ=255)
               tAAAGATGCGGGCGTCGGCATGATTGGCGCGGGCATTGTGCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTa                                                                                                    >  2:384288/1‑140 (MQ=255)
               tAAAGATGCGGGCGTCGGCATGATTGGCGCGGGCATTGTGCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCt                                                                                            >  1:96430/1‑148 (MQ=255)
                                gCATGATTGGCGCGGGCATTGTGCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGcc                                                                                                       >  1:425114/1‑120 (MQ=255)
                                gCATGATTGGCGCGGGCATTGTGCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGcc                                                                                                       <  2:425114/120‑1 (MQ=255)
                                       tGGCGCGGGCATTGTGCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACg                                                                                                         <  4:1020912/111‑1 (MQ=255)
                                       tGGCGCGGGCATTGTGCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACg                                                                                                         >  3:1020912/1‑111 (MQ=255)
                                         gcgcGGGCATTGTGCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCACGCCGCATccggca                                                                                                               <  1:1003500/103‑2 (MQ=255)
                                         gcgcGGGCATTGTGCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCACGCCGCATccggca                                                                                                               >  2:1003500/1‑102 (MQ=255)
                                                   tgtgCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTa                                                                                           >  1:896628/1‑113 (MQ=255)
                                                   tgtgCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTa                                                                                           <  2:896628/113‑1 (MQ=255)
                                                      gCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATg                                                                                     <  4:1047304/116‑1 (MQ=255)
                                                      gCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATg                                                                                     >  3:1047304/1‑116 (MQ=255)
                                                        aTCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTa                                                                                           >  3:793681/1‑108 (MQ=255)
                                                        aTCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTa                                                                                           <  4:793681/108‑1 (MQ=255)
                                                        aTCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCACGCCGCATCCGGCATGa                                                                                                            >  4:621426/1‑91 (MQ=255)
                                                        aTCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCACGCCGCATCCGGCATGa                                                                                                            <  3:621426/91‑1 (MQ=255)
                                                          ccACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATgcggcg                                                                                                               <  4:1024447/86‑1 (MQ=255)
                                                          ccACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATgcggcg                                                                                                               >  3:1024447/1‑86 (MQ=255)
                                                          ccACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATgcgg                                                                                                                 >  2:435718/1‑84 (MQ=255)
                                                          ccACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATgcgg                                                                                                                 <  1:435718/84‑1 (MQ=255)
                                                            aCCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCgg                                                                                               >  2:439136/1‑100 (MQ=255)
                                                            aCCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCgg                                                                                               <  1:439136/100‑1 (MQ=255)
                                                                 ttGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAt                                          <  3:67107/148‑1 (MQ=255)
                                                                      tGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTAt                                                                                                   <  1:214636/86‑1 (MQ=255)
                                                                      tGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTAt                                                                                                   >  2:214636/1‑86 (MQ=255)
                                                                      tGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCa                                                                                <  4:743984/105‑1 (MQ=255)
                                                                      tGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCa                                                                                >  3:743984/1‑105 (MQ=255)
                                                                        cTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATgcg                                                                                                                  >  4:652027/1‑69 (MQ=255)
                                                                        cTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATgcg                                                                                                                  <  3:652027/69‑1 (MQ=255)
                                                                         ttCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATgcggcg                                                                                                               >  2:915692/1‑71 (MQ=255)
                                                                         ttCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATgcggcg                                                                                                               <  1:915692/71‑1 (MQ=255)
                                                                          tCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTa                                                                      <  3:506611/111‑1 (MQ=255)
                                                                          tCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTa                                                                      >  4:506611/1‑111 (MQ=255)
                                                                          tCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTa                                                       >  2:611576/1‑126 (MQ=255)
                                                                          tCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTa                                                       <  1:611576/126‑1 (MQ=255)
                                                                            gagaAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTa                                                       >  4:828883/1‑124 (MQ=255)
                                                                            gagaAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTa                                                       <  3:828883/124‑1 (MQ=255)
                                                                               aaaGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATgcggcg                                                                                                               <  3:254883/65‑1 (MQ=255)
                                                                               aaaGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATgcggcg                                                                                                               >  4:254883/1‑65 (MQ=255)
                                                                                  gCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGaa                                                                                                           <  2:463266/66‑1 (MQ=255)
                                                                                  gCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGaa                                                                                                           >  1:463266/1‑66 (MQ=255)
                                                                                     aTTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAgcgc                                                   >  3:1114683/1‑119 (MQ=255)
                                                                                     aTTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAgcgc                                                   <  4:1114683/119‑1 (MQ=255)
                                                                                            gCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGt                                                                                                              <  2:543586/53‑1 (MQ=255)
                                                                                            gCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGt                                                                                                              >  1:543586/1‑53 (MQ=255)
                                                                                                          tACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCa                                               >  4:855548/1‑102 (MQ=255)
                                                                                                          tACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCa                                               <  3:855548/102‑1 (MQ=255)
                                                                                                                      ccTACATGTTCATGCCGGATGCGGCGTGAACGCCTTAt                                                                                                   >  3:872425/1‑38 (MQ=255)
                                                                                                                         aCATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCgg  >  1:332342/1‑132 (MQ=255)
                                                                                                                         aCATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCgg  <  2:332342/132‑1 (MQ=255)
                                                                                                                                   gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACg            >  1:925353/1‑112 (MQ=255)
                                                                                                                                   gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACg            <  2:925353/112‑1 (MQ=40)
                                                                                                                                          |                                                                                                                  
CACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGGCGCGGGCATTGTGCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGG  >  NC_000913/339626‑339878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: