Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,817,949 A→C 9.3% I64L (ATC→CTC)  dinD → DNA damage‑inducible protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,817,9490AC 9.3% 976.5 / 81.7 290I64L (ATC→CTC) dinDDNA damage‑inducible protein
Reads supporting (aligned to +/- strand):  ref base A (130/133);  new base C (14/13);  total (144/146)
Fisher's exact test for biased strand distribution p-value = 8.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.58e-02

Only 100 of 301 total aligned reads displayed.

CGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATCCAGA  >  NC_000913/3817810‑3818096
                                                                                                                                           |                                                                                                                                                   
cGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGat                                                                                                                                             <  2:811560/148‑1 (MQ=255)
         aCAATGGTCAGCAAGACAACTTGGGACACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCaaa                                                                                                                                    <  1:489737/148‑1 (MQ=255)
              ggTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATATCGAGGATCTCCTCGAAATgg                                                                                                                                         <  3:633781/138‑1 (MQ=255)
                    cAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGatat                                                                                                                                           >  4:930838/1‑130 (MQ=255)
                     aaGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAAt                                                                                                                                  <  4:306235/138‑1 (MQ=255)
                      aGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTg                                                                                                                                >  2:979885/1‑139 (MQ=255)
                            cTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCaaaa                                                                                                                 >  3:247593/1‑148 (MQ=255)
                              tGGGAAACTACTGGGTTATTCCGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGAt                                                                                                                                                    <  3:1079274/111‑1 (MQ=255)
                              tGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGatat                                                                                                                                           >  4:635954/1‑120 (MQ=255)
                              tGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGat                                                                                                                                             >  4:646112/1‑118 (MQ=255)
                              tGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAaga                                                                                                               >  2:497579/1‑148 (MQ=255)
                                       actGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAATGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATATCGAGGATCTCCTCGa                                                                                                                                              >  2:328514/1‑108 (MQ=255)
                                            gTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCg                                                                                                                                               <  2:589269/102‑1 (MQ=255)
                                            gTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACAt                                                                                                 <  4:1139716/148‑1 (MQ=255)
                                                 tCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATgg                                                                                                                                         <  2:1064631/103‑1 (MQ=255)
                                                 tCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAACTGATGACCATTTCGAGGAGATCCTCGATATgg                                                                                                                                         <  3:721113/103‑1 (MQ=255)
                                                    gagTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTaaa                                                                                                       >  4:1080127/1‑134 (MQ=255)
                                                          cGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCaa                                                                                                                          >  3:740380/1‑109 (MQ=255)
                                                          cGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACa                                                                                                  >  3:804951/1‑133 (MQ=255)
                                                            tCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTaaa                                                                                                       <  2:122607/126‑1 (MQ=255)
                                                            tCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTAt                                                                                 >  4:592821/1‑148 (MQ=255)
                                                             cACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCt                                                                                                                                                 >  4:611463/1‑83 (MQ=255)
                                                             cACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCt                                                                                                                                                 <  3:611463/83‑1 (MQ=255)
                                                             cACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGata                                                                                                                                            >  4:70392/1‑88 (MQ=255)
                                                             cACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATg                                                                                <  3:592821/148‑1 (MQ=255)
                                                                tttATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCg                                                                                                                                               >  3:121098/1‑82 (MQ=255)
                                                                tttATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTAGCGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGcca                                                                             >  3:498736/1‑147 (MQ=255)
                                                                       cTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATcc                                                                                                                                                  <  4:35930/72‑1 (MQ=255)
                                                                        tGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCaaaa                                                                                                                                   >  4:879605/1‑86 (MQ=255)
                                                                        tGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAAtt                                                                                                                                 <  4:810875/88‑1 (MQ=255)
                                                                        tGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGcc                                                                              >  4:1008763/1‑139 (MQ=255)
                                                                        tGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTAcc                                                                       <  2:387815/146‑1 (MQ=255)
                                                                        tGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTAcc                                                                       >  1:387815/1‑146 (MQ=255)
                                                                        tGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTAcc                                                                       >  2:1028923/1‑146 (MQ=255)
                                                                        tGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTg                                                                     >  4:127555/1‑148 (MQ=255)
                                                                           aTTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGat                                                                                                                                             <  3:603831/73‑1 (MQ=255)
                                                                           aTTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGat                                                                                                                                             >  4:603831/1‑73 (MQ=255)
                                                                           aTTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGa                                                                                                                                              <  4:528210/72‑1 (MQ=255)
                                                                           aTTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGc                                                                                                             <  2:846009/105‑1 (MQ=255)
                                                                           aTTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACAt                                                                                                 >  4:1059558/1‑117 (MQ=255)
                                                                              aaCGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACAt                                                                                                 <  1:428429/114‑1 (MQ=255)
                                                                              aaCGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACAt                                                                                                 >  2:428429/1‑114 (MQ=255)
                                                                                cgcgcgCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAag                                                                                                                <  4:431675/97‑1 (MQ=255)
                                                                                cgcgcgCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTAcc                                                                       >  2:479550/1‑138 (MQ=255)
                                                                                  cgcgcCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGc                                                                                                             >  1:210930/1‑98 (MQ=255)
                                                                                  cgcgcCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGt                                                                                              >  2:964017/1‑113 (MQ=255)
                                                                                      ccAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATg                                                                                >  1:909877/1‑123 (MQ=255)
                                                                                       cAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCt                                                                                                                                                 >  2:1112422/1‑57 (MQ=255)
                                                                                        aaaGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTa                                                                                  >  1:64998/1‑119 (MQ=255)
                                                                                        aaaGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGcc                                                                              >  2:169393/1‑123 (MQ=255)
                                                                                        aaaGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTg                                                                            >  3:924925/1‑125 (MQ=255)
                                                                                         aagaagCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTgg                                                                                                                               <  3:360757/73‑1 (MQ=255)
                                                                                          agaagCCTGTGAGAGCAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTAc                                                                        >  4:844112/1‑127 (MQ=255)
                                                                                          agaagCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATcc                                                                                                                                                  >  4:46728/1‑53 (MQ=255)
                                                                                          agaagCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAaga                                                                                                               >  4:941762/1‑88 (MQ=255)
                                                                                              gCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTg                                               <  4:568140/148‑1 (MQ=255)
                                                                                                cTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTg                                                                                                                                <  4:908273/65‑1 (MQ=255)
                                                                                                cTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCg                                                                                     <  1:955415/108‑1 (MQ=255)
                                                                                                 tgtgAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATgg                                                                                                                                         <  1:476326/55‑1 (MQ=255)
                                                                                                  gtgAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGgtagta                                                               <  1:31895/128‑1 (MQ=255)
                                                                                                  gtgAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACaaa                                                           <  3:1022946/132‑1 (MQ=255)
                                                                                                    gagaACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTg                                                                                                                                <  3:906548/61‑1 (MQ=255)
                                                                                                    gagaACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGgt                                                                   >  1:691680/1‑122 (MQ=255)
                                                                                                      gaACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCt                                                                      >  1:232324/1‑117 (MQ=255)
                                                                                                       aaCAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTg                                                                     >  3:644985/1‑117 (MQ=255)
                                                                                                           gTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCa                                                                                                            >  3:84251/1‑74 (MQ=255)
                                                                                                            tGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGAc                                                                                                   <  1:162758/82‑1 (MQ=255)
                                                                                                             ggTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTaaa                                                                                                       >  4:756841/1‑77 (MQ=255)
                                                                                                               tCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGcc                                                                              <  1:55159/100‑1 (MQ=255)
                                                                                                                cacacaATTGATGACCATTTCGAGGAGATCCTCGatat                                                                                                                                           <  3:960629/38‑1 (MQ=255)
                                                                                                                  cacaATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGt                                                                           >  2:541064/1‑100 (MQ=255)
                                                                                                                  cacaATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTAcc                                                                       >  4:202012/1‑104 (MQ=255)
                                                                                                                   acaATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCggcag                          <  2:74153/148‑1 (MQ=255)
                                                                                                                    caATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCg                                                                                               >  1:971840/1‑78 (MQ=255)
                                                                                                                      aTTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTaaa                                                                                                       >  3:413411/1‑68 (MQ=255)
                                                                                                                      aTTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTaaa                                                                                                       <  1:119515/68‑1 (MQ=255)
                                                                                                                        tgatgaCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCggcagggcag                     <  4:364629/148‑1 (MQ=255)
                                                                                                                            gaCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGt                                     <  2:265008/128‑1 (MQ=255)
                                                                                                                             aCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAag                                                                                                                >  3:87833/1‑52 (MQ=255)
                                                                                                                             aCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCggca                           >  2:645180/1‑137 (MQ=255)
                                                                                                                             aCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCg                              >  1:755246/1‑134 (MQ=255)
                                                                                                                              ccATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTg                                                                     <  3:464253/94‑1 (MQ=255)
                                                                                                                              ccATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGaaa                                          >  1:1103867/1‑121 (MQ=255)
                                                                                                                              ccATTTCGAGAAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGt                                                                           >  1:836857/1‑88 (MQ=255)
                                                                                                                               cATATCGAGGATCTCCTCGAAATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCggca                           >  3:564542/1‑135 (MQ=255)
                                                                                                                                atatCGAGGATCTCCTCGAAATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACaaa                                                           <  1:608981/99‑1 (MQ=39)
                                                                                                                                aTTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACg                                                        <  3:936149/105‑1 (MQ=255)
                                                                                                                                 tatCGAGGATCTCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGc                                                                                    <  2:924512/74‑1 (MQ=38)
                                                                                                                                  ttCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACAt                                                                                                 <  1:51145/62‑1 (MQ=255)
                                                                                                                                  atCGAGGATCTCCTCGAAATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGgt                                                                   >  4:721580/2‑92 (MQ=38)
                                                                                                                                    cGAGGATCTCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGgtagt                                                                >  1:1010767/1‑93 (MQ=255)
                                                                                                                                      aggagATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATg                                                                                >  3:676447/1‑75 (MQ=255)
                                                                                                                                      aggagATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCggcag                          <  3:177551/129‑1 (MQ=255)
                                                                                                                                      aggagATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTAt       >  4:1032438/1‑148 (MQ=255)
                                                                                                                                      aggagATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTAt       <  2:67023/148‑1 (MQ=255)
                                                                                                                                      aggagATCCTCGAGATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTgg                                                                    <  1:1060916/87‑1 (MQ=255)
                                                                                                                                       ggagATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTa                                                                                  >  3:1118232/1‑72 (MQ=255)
                                                                                                                                        gagaTCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGgt                                                                   <  2:215451/86‑1 (MQ=255)
                                                                                                                                        gagaTCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAAcc                                        >  3:104944/1‑113 (MQ=255)
                                                                                                                                           aTCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATCCAGa  <  3:345057/148‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
CGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATCCAGA  >  NC_000913/3817810‑3818096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: