Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,832,188 A→T 16.0% intergenic (+22/+31) yicH → / ← yicI putative inner membrane‑anchored periplasmic AsmA family protein/putative alpha‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,832,1880AT16.0% 512.3 / 81.4 181intergenic (+22/+31)yicH/yicIputative inner membrane‑anchored periplasmic AsmA family protein/putative alpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base A (76/76);  new base T (15/14);  total (91/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

Only 100 of 187 total aligned reads displayed.

CCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGT  >  NC_000913/3832041‑3832335
                                                                                                                                                   |                                                                                                                                                   
      aaaCCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAgt                                                                                                                                               >  1:1122749/1‑147 (MQ=255)
             cGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTAccc                                                                                                                                        <  2:1122749/148‑1 (MQ=255)
               gTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACt                                                                                                                                                   >  2:182954/1‑135 (MQ=255)
                      ggCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACt                                                                                                                                                   >  2:597570/1‑128 (MQ=255)
                          aTTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTAcc                                                                                                                                         <  1:558624/134‑1 (MQ=255)
                          aTTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGa                                                                                                                                               <  4:851750/128‑1 (MQ=255)
                          aTTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGa                                                                                                                                               >  3:851750/1‑128 (MQ=255)
                                tGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTAcc                                                                                                                                         <  4:507220/128‑1 (MQ=255)
                                tGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTAcc                                                                                                                                         >  3:507220/1‑128 (MQ=255)
                                tGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTAcc                                                                                                                                         >  1:98369/1‑128 (MQ=255)
                                 gCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTAcc                                                                                                                                         >  2:612542/1‑127 (MQ=255)
                                             gcAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGt                                                                                                        <  4:115257/148‑1 (MQ=255)
                                             gcAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGt                                                                                                        >  3:115257/1‑148 (MQ=255)
                                              cAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTACCCg                                                                                                                                       <  2:1076819/116‑1 (MQ=255)
                                              cAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTACCCg                                                                                                                                       >  1:1076819/1‑116 (MQ=255)
                                                     gcaAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTa                                                                                                                                           >  4:440077/1‑105 (MQ=255)
                                                     gcaAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTa                                                                                                                                           <  3:440077/105‑1 (MQ=255)
                                                       aaGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGAGGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGa                                                                                                                                               >  2:966782/1‑99 (MQ=255)
                                                          tGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGa                                                                                                                                               >  2:395581/1‑96 (MQ=255)
                                                          tGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGa                                                                                                                                               <  1:395581/96‑1 (MQ=255)
                                                               cAAACCATGAGTGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAgg                                                                                      >  3:987248/1‑148 (MQ=255)
                                                               cAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACa                                                                                                                  <  1:1049251/120‑1 (MQ=255)
                                                               cAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGa                                                                                        >  3:590521/1‑146 (MQ=255)
                                                               cAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGa                                                                                        <  4:590521/146‑1 (MQ=255)
                                                                 aaCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAg                                                                                                                                                >  1:182887/1‑88 (MQ=255)
                                                                   ccATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACt                                                                                                                                                   <  3:851706/83‑1 (MQ=255)
                                                                   ccATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGa                                                                                                                                               >  2:845420/1‑87 (MQ=255)
                                                                   ccATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGa                                                                                                                                               >  1:922419/1‑87 (MQ=255)
                                                                   ccATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCAGGTACGACTCAGa                                                                                                                                               <  1:845420/87‑1 (MQ=255)
                                                                    cATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCtg                                                                                                                                                  <  4:1073837/83‑2 (MQ=255)
                                                                    cATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTa                                                                                                                                           >  4:992933/1‑90 (MQ=255)
                                                                    cATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTAccc                                                                                                                                        <  3:306319/93‑1 (MQ=255)
                                                                    cATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTAccc                                                                                                                                        >  4:306319/1‑93 (MQ=255)
                                                                    cATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGATGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTa                                                                                                                                           <  3:992933/90‑1 (MQ=255)
                                                                      tGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTAcc                                                                                                                                         <  1:691746/90‑1 (MQ=255)
                                                                      tGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTAcc                                                                                                                                         >  2:691746/1‑90 (MQ=255)
                                                                      tGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAg                                                                                                                        >  1:285705/1‑107 (MQ=255)
                                                                       gAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCATGAGGCTTcacca                                                                              <  1:846443/148‑1 (MQ=255)
                                                                          tGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGa                                                                                                                                               >  3:541336/1‑80 (MQ=255)
                                                                          tGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTcc                                                                                             >  4:812017/1‑130 (MQ=255)
                                                                           gCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTg                                                                                         >  3:133161/1‑133 (MQ=255)
                                                                           gCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTg                                                                                         <  4:133161/133‑1 (MQ=255)
                                                                              ggCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGt                                                                                                        >  1:1063365/1‑115 (MQ=255)
                                                                              ggCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGt                                                                                                        <  2:1063365/115‑1 (MQ=255)
                                                                                cATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACg                                                                                                                                             <  3:642769/76‑1 (MQ=255)
                                                                                cATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACg                                                                                                                                             >  4:642769/1‑76 (MQ=255)
                                                                                cATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTAcc                                                                                                                                         >  1:14792/1‑80 (MQ=255)
                                                                                cATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCa                                                                                                      <  2:325364/115‑1 (MQ=255)
                                                                                cATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGccc                                                                       >  4:777846/1‑146 (MQ=255)
                                                                                  tCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCa                                                                                                                               >  2:131384/1‑88 (MQ=255)
                                                                                  tCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTcac                                                                                >  1:408690/1‑135 (MQ=255)
                                                                                  tCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTcac                                                                                <  2:408690/135‑1 (MQ=255)
                                                                                   cGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTaa                                                                                                            >  2:82387/1‑106 (MQ=255)
                                                                                   cGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTaa                                                                                                            <  1:82387/106‑1 (MQ=255)
                                                                                     tttCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCa                                                                <  1:379036/148‑1 (MQ=255)
                                                                                      ttCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTa                                                                                                                                           <  2:733202/72‑1 (MQ=255)
                                                                                      taccAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTa                                                                                                                                           >  1:733202/3‑72 (MQ=255)
                                                                                          aGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCg                                                                                                                                       <  2:11578/72‑1 (MQ=255)
                                                                                          aGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTc                                                           <  1:204947/148‑1 (MQ=255)
                                                                                              gTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCa                                                                <  4:1003024/139‑1 (MQ=255)
                                                                                              gTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCa                                                                >  3:1003024/1‑139 (MQ=255)
                                                                                               tGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACt                                                              >  1:116328/1‑140 (MQ=255)
                                                                                                 aaGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAAt                                                                                                           <  2:116837/93‑1 (MQ=255)
                                                                                                 aaGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGAAGTACCCGGCATTCCATCGATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCTCTTTCAGCCTGc                                                    <  4:987248/148‑1 (MQ=255)
                                                                                                    ttACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTg                                                                                         <  4:897713/108‑1 (MQ=255)
                                                                                                    ttACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTg                                                                                         >  3:897713/1‑108 (MQ=255)
                                                                                                     tACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTc                                                           >  4:334162/1‑137 (MQ=255)
                                                                                                     tACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTc                                                           <  3:334162/137‑1 (MQ=255)
                                                                                                         tCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCa                                                                                                 >  3:767050/1‑95 (MQ=255)
                                                                                                         tCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTcc                                                                                             <  1:602185/99‑1 (MQ=255)
                                                                                                           gACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCtt                                          >  2:152521/1‑148 (MQ=255)
                                                                                                            aCGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTg                                                                                                         >  1:450306/1‑84 (MQ=255)
                                                                                                            aCGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTg                                                                                                         <  2:387592/84‑1 (MQ=255)
                                                                                                            aCGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTg                                                                                                         <  2:450306/84‑1 (MQ=255)
                                                                                                             cGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGcc                                                       >  3:108422/1‑133 (MQ=255)
                                                                                                                 gCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGt                                             >  1:127111/1‑139 (MQ=255)
                                                                                                                   cGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTcaccac                                                                             <  2:501218/105‑1 (MQ=255)
                                                                                                                           tAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTc                                                                                                       >  1:6222/1‑71 (MQ=255)
                                                                                                                            aaaCATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTc                         >  4:516260/1‑148 (MQ=255)
                                                                                                                                 tCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTa                              >  2:876359/1‑138 (MQ=255)
                                                                                                                                  cAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGa                                                                                        >  4:387078/1‑79 (MQ=255)
                                                                                                                                  cAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGa                                                                                        <  3:387078/79‑1 (MQ=255)
                                                                                                                                  cAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGAcc                                   >  4:835130/1‑132 (MQ=255)
                                                                                                                                    aTCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGa                                                                                                                       <  3:829237/46‑1 (MQ=255)
                                                                                                                                    aTCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACt                                                              <  3:65641/103‑1 (MQ=255)
                                                                                                                                    aTCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACt                                                              >  4:65641/1‑103 (MQ=255)
                                                                                                                                        cGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTg                                                                                         <  3:785449/72‑1 (MQ=255)
                                                                                                                                          ccGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAAc                                                >  2:262042/1‑111 (MQ=255)
                                                                                                                                            gggTACGTCTGAGTCGTACCCGGCATTCCATCAATAGTTTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGa                                     <  4:766584/120‑1 (MQ=255)
                                                                                                                                             ggTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCCTTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGt                                             <  3:944025/111‑1 (MQ=255)
                                                                                                                                             ggTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGt                                             >  4:944025/1‑111 (MQ=255)
                                                                                                                                               tACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTc                                                                                                       >  3:1064701/1‑51 (MQ=255)
                                                                                                                                               tACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTc                                                                                                       <  4:1064701/51‑1 (MQ=255)
                                                                                                                                                aCGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAg     <  4:680890/148‑1 (MQ=255)
                                                                                                                                                aCGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAg     >  3:680890/1‑148 (MQ=255)
                                                                                                                                                aCGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAg     >  1:13786/1‑148 (MQ=255)
                                                                                                                                                 cGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGc                                        >  1:484554/1‑112 (MQ=255)
                                                                                                                                                   aCTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCt                                           <  2:1090620/107‑1 (MQ=255)
                                                                                                                                                   aCTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCt                                           >  1:1090620/1‑107 (MQ=255)
                                                                                                                                                   aCTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAgtgt  >  4:794217/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGT  >  NC_000913/3832041‑3832335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: