Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,852,558 T→A 5.3% K76I (AAA→ATA) ‡ ilvB ← acetolactate synthase 2 large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,852,5580TA 5.3% 428.6 / 9.4 114K76I (AAA→ATA) ‡ilvBacetolactate synthase 2 large subunit
Reads supporting (aligned to +/- strand):  ref base T (52/56);  new base A (3/3);  total (55/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 116 total aligned reads displayed.

ACTTCCTGGAAGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTG  >  NC_000913/3852411‑3852690
                                                                                                                                                   |                                                                                                                                    
aCTTCCTGGAAGGCGTCGGTGCCGATCATCGAGGCTGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGtt                                                                                                                                      <  4:741080/148‑1 (MQ=255)
aCTTCCTGGAAGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGtt                                                                                                                                      >  3:49721/1‑148 (MQ=255)
         aaGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCgg                                                                                                                             <  1:707266/148‑1 (MQ=255)
                cGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTgcgc                                                                                                                        >  3:994074/1‑146 (MQ=255)
                      cGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTcc                                                                                                                <  3:130051/148‑1 (MQ=255)
                        atcatcGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCt                                                                                                              >  3:715346/1‑148 (MQ=255)
                        atcatcGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCt                                                                                                              >  1:410563/1‑148 (MQ=255)
                        atcatcGAGGCAGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTgcgcg                                                                                                                       <  2:1128558/139‑1 (MQ=255)
                        atcatcGAGGCAGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTgcgcg                                                                                                                       >  1:1128558/1‑139 (MQ=255)
                         tcatcGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCACGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTg                                                                                                             >  1:984882/1‑148 (MQ=255)
                          catcGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGa                                                                                                            <  1:49092/148‑1 (MQ=255)
                             cGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCg                                                                                                         <  4:403769/148‑1 (MQ=255)
                              gAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGa                                                                                                        <  2:410563/148‑1 (MQ=255)
                               aGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCgg                                                                                                                             >  4:592393/1‑126 (MQ=255)
                               aGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCgg                                                                                                                             <  3:592393/126‑1 (MQ=255)
                                  cGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATaaa                                                                                                    >  1:74060/1‑148 (MQ=255)
                                   gggAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTc                                                                                                                               >  4:77743/1‑120 (MQ=255)
                                   gggAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTc                                                                                                                               <  3:77743/120‑1 (MQ=255)
                                    ggAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGc                                                                                                  <  4:880701/148‑1 (MQ=255)
                                    ggAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGc                                                                                                  >  4:79590/1‑148 (MQ=255)
                                     gAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGcc                                                                                                 <  3:744640/148‑1 (MQ=255)
                                             ccAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAg                                                                                                           <  3:320422/130‑1 (MQ=255)
                                             ccAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAg                                                                                                           >  4:320422/1‑130 (MQ=255)
                                              cAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTa                                                                                                                                    <  2:217051/104‑1 (MQ=255)
                                              cAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTa                                                                                                                                    >  1:217051/1‑104 (MQ=255)
                                              cAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTg                                                                                        <  2:984882/148‑1 (MQ=255)
                                                   aTGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCAt                                                                                   <  3:79590/148‑1 (MQ=255)
                                                         aTCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACg                                                                               >  3:955770/1‑146 (MQ=255)
                                                         aTCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACg                                                                               <  4:955770/146‑1 (MQ=255)
                                                          tCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGTGATAAAGCCCGCGCCCTGTTCATGACGGGc                                                                            >  1:876573/1‑148 (MQ=255)
                                                              cGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACg                                                                               >  1:177820/1‑141 (MQ=255)
                                                              cGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACg                                                                               <  2:177820/141‑1 (MQ=255)
                                                                      aGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCg                                                                >  3:694694/1‑148 (MQ=255)
                                                                       gTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTc                                                                                                                 <  1:895861/98‑1 (MQ=255)
                                                                       gTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTc                                                                                                                 >  2:895861/1‑98 (MQ=255)
                                                                        tCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCa                                                                                    >  4:485763/1‑126 (MQ=255)
                                                                        tCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCa                                                                                    <  3:485763/126‑1 (MQ=255)
                                                                        tCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGc                                                                            >  3:1031370/1‑134 (MQ=255)
                                                                        tCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGc                                                                            <  4:1031370/134‑1 (MQ=255)
                                                                           aGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATg                                                                   >  2:358005/1‑140 (MQ=255)
                                                                           aGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATg                                                                   <  1:358005/140‑1 (MQ=255)
                                                                           aGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGAttt                                                           >  3:954367/1‑148 (MQ=255)
                                                                            gCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTa                                                                                                                                    >  3:194165/1‑74 (MQ=255)
                                                                            gCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTa                                                                                                                                    <  4:194165/74‑1 (MQ=255)
                                                                            gCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTg                                                          <  2:74060/148‑1 (MQ=255)
                                                                             ccGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTg                                                                                                             >  4:926237/1‑96 (MQ=255)
                                                                             ccGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTg                                                                                                             <  3:926237/96‑1 (MQ=255)
                                                                                  cATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTg                                                                                        <  1:150043/112‑1 (MQ=255)
                                                                                     cGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGTGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTg                                                 <  2:876573/148‑1 (MQ=255)
                                                                                      ggCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTgg                                                <  4:954367/148‑1 (MQ=255)
                                                                                      ggCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTgg                                                <  3:359861/148‑1 (MQ=255)
                                                                                       gCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGa                                                                        >  1:845142/1‑123 (MQ=255)
                                                                                       gCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGa                                                                        <  2:845142/123‑1 (MQ=255)
                                                                                          aTGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGa                                                                        <  4:623646/120‑1 (MQ=255)
                                                                                          aTGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGa                                                                        >  3:623646/1‑120 (MQ=255)
                                                                                           tGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTaa                                           <  3:916000/148‑1 (MQ=255)
                                                                                              cGGTCACCAGGTTAGTCGCAGCCGGTCCGCTACAGGCCATATAGACCGACGGTAAACCGGCGGt                                                                                                                            <  4:945079/64‑1 (MQ=25)
                                                                                              cGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGAtt                                                            <  2:999823/128‑1 (MQ=255)
                                                                                              cGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGAtt                                                            >  1:999823/1‑128 (MQ=255)
                                                                                              cGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGACGGTAAACCGGCGGt                                                                                                                            >  3:945079/1‑64 (MQ=37)
                                                                                                gTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGa                                                                        >  1:1091930/1‑114 (MQ=255)
                                                                                                gTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGa                                                                        <  2:1091930/114‑1 (MQ=255)
                                                                                                         ttAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAAcag                             >  4:483848/1‑148 (MQ=255)
                                                                                                          tAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGCGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAAcagg                            >  2:1111176/1‑148 (MQ=255)
                                                                                                              cGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAAcaggcagg                        <  4:694694/148‑1 (MQ=255)
                                                                                                                cACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTgg                                                <  2:1008367/122‑1 (MQ=255)
                                                                                                                cACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTgg                                                >  1:1008367/1‑122 (MQ=255)
                                                                                                                  cccGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCgcgc                                                                                            <  2:568940/76‑1 (MQ=255)
                                                                                                                  cccGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGa                       >  3:1026636/1‑145 (MQ=255)
                                                                                                                  cccGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGa                       <  4:1026636/145‑1 (MQ=255)
                                                                                                                   ccGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGa                   <  3:483848/148‑1 (MQ=255)
                                                                                                                        ccGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGccc                                                                                          >  4:1013401/1‑72 (MQ=255)
                                                                                                                        ccGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGccc                                                                                          <  3:1013401/72‑1 (MQ=255)
                                                                                                                        ccGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGc              >  4:858346/1‑148 (MQ=255)
                                                                                                                          gCTACAGGCCATAGAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCAt                                      >  3:82921/1‑122 (MQ=255)
                                                                                                                          gCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCAt                                      <  4:82921/122‑1 (MQ=255)
                                                                                                                          gCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGccc            <  4:672685/148‑1 (MQ=255)
                                                                                                                           cTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAAcaggc                           <  1:9206/132‑1 (MQ=255)
                                                                                                                           cTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAAcaggc                           >  2:9206/1‑132 (MQ=255)
                                                                                                                           cTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGa                   <  3:1011523/140‑1 (MQ=255)
                                                                                                                           cTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGa                   >  4:1011523/1‑140 (MQ=255)
                                                                                                                                 gCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGc     >  3:968759/1‑148 (MQ=255)
                                                                                                                                  ccATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTg                                                                                                             <  3:314636/43‑1 (MQ=255)
                                                                                                                                  ccATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTg                                                                                                             >  4:314636/1‑43 (MQ=255)
                                                                                                                                  ccATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCAt                                      >  3:1079830/1‑114 (MQ=255)
                                                                                                                                  ccATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCAt                                      <  4:1079830/114‑1 (MQ=255)
                                                                                                                                  ccATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAAcaggc                           >  2:315767/1‑125 (MQ=255)
                                                                                                                                  ccATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAAcaggc                           <  1:315767/125‑1 (MQ=255)
                                                                                                                                    aTACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGaa                  <  2:230152/132‑1 (MQ=255)
                                                                                                                                    aTACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGaa                  >  1:230152/1‑132 (MQ=255)
                                                                                                                                    aTACAGACCGCCGGTTTACCGTCGGCGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTg  <  1:1111176/148‑1 (MQ=255)
                                                                                                                                     tACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACg                                                                               >  4:612135/1‑70 (MQ=255)
                                                                                                                                     tACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACg                                                                               <  3:612135/70‑1 (MQ=255)
                                                                                                                                       cgcaCCGACGGTAAACCGGCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTgg                                                >  2:363907/4‑99 (MQ=255)
                                                                                                                                       cgcaCCGACGGTAAACCGGCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTgg                                                <  1:363907/96‑1 (MQ=255)
                                                                                                                                           ccgacgGTAAACCGGCGGTGCGCGCCATTCCCTGAg                                                                                                           <  3:945147/36‑1 (MQ=9)
                                                                                                                                           ccgacgGTAAACCGGCGGTGCGCGCCATTCCCTGAg                                                                                                           >  4:945147/1‑36 (MQ=12)
                                                                                                                                             gccgGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGc                                                     <  4:962918/88‑1 (MQ=255)
                                                                                                                                              ccgGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTTGCTTAAGGCa                                       <  3:669190/101‑1 (MQ=255)
                                                                                                                                              ccgGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCa                                       >  4:669190/1‑101 (MQ=255)
                                                                                                                                                   |                                                                                                                                    
ACTTCCTGGAAGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTG  >  NC_000913/3852411‑3852690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: