Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,935,454 A→C 5.4% N56T (AAC→ACC)  rbsC → D‑ribose ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,935,4540AC 5.4% 413.6 / 12.7 112N56T (AAC→ACC) rbsCD‑ribose ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (56/50);  new base C (3/3);  total (59/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

Only 100 of 114 total aligned reads displayed.

TTATTTCACGAAAGCGTGGCTGATGGAGCAGAAATCGCTTATCGCTCTGCTGGTGCTGATCGCGATTGTCTCGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGACCGGCGCAGTTGCTGCATCTATCGTCGGCATTGAAGTCAATGCGCTGGTGGCTGTCGCTGC  >  NC_000913/3935317‑3935595
                                                                                                                                         |                                                                                                                                             
ttATTTCACGAAAGCGTGGCTGATGGAGCAGAAATCGCTTATCGCTCTGCTGGTGCTGATCGCGATTGTCTCGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCa                                                                                                                                          >  1:148309/1‑143 (MQ=255)
          aaaGCGTGGCTGATGGAGCAGAAATCGCTTATCGCTCTGCTGGTGCTGATCGCGATTGTCTCGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCGATATTCTCCAGCAAACCTCAGTGAACGCCATTa                                                                                                                                       >  2:829664/1‑136 (MQ=255)
                      aTGGAGCAGAAATCGCTTATCGCTCTGCTGGTGCTGATCGCGATTGTCTCGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGcc                                                                                                                                           <  3:735856/120‑1 (MQ=255)
                          aGCAGAAATCGCTTATCGCTCTGCTGGTGCTGATCGCGATTGTCTCGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGaa                                                                                                                                               >  3:1054869/1‑112 (MQ=255)
                          aGCAGAAATCGCTTATCGCTCTGCTGGTGCTGATCGCGATTGTCTCGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGaa                                                                                                                                               <  4:1054869/112‑1 (MQ=255)
                                 aTCGCTTATCGCTCTGCTGGTGCTGATCGCGATTGTCTCGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATg                                                                                                                                     >  2:1006854/1‑115 (MQ=255)
                                 aTCGCTTATCGCTCTGCTGGTGCTGATCGCGATTGTCTCGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATg                                                                                                                                     <  1:1006854/115‑1 (MQ=255)
                                     cTTATCGCTCTGCTGGTGCTGATCGCGATTGTCTCGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCt                                                                                                           >  2:948510/1‑137 (MQ=255)
                                     cTTATCGCTCTGCTGGTGCTGATCGCGATTGTCTCGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCt                                                                                                           <  1:948510/137‑1 (MQ=255)
                                         tCGCTCTGCTGGTGCTGATCGCGATTGTCTCGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCTGTCGGGATGACGCTgg                                                                                                                  >  4:84458/1‑126 (MQ=255)
                                          cGCTCTGCTGGTGCTGATCGCGATTGTCTCGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGACCGCCATAATGGCgtt                                                                                                                                <  2:423822/111‑3 (MQ=255)
                                             tctGCTGGTGCTGATCGCGATTGTCTCGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTc                                                                                                                               >  2:778447/1‑109 (MQ=255)
                                             tctGCTGGTGCTGATCGCGATTGTCTCGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTc                                                                                                                               <  1:778447/109‑1 (MQ=255)
                                                       cTGATCGCGATTGTCTCGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGAt                                                                                                              >  4:190651/1‑116 (MQ=255)
                                                       cTGATCGCGATTGTCTCGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGAt                                                                                                              <  3:190651/116‑1 (MQ=255)
                                                        tGATCGCGATTGTCTCGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTg                                                                                                                   >  3:691975/1‑110 (MQ=255)
                                                        tGATCGCGATTGTCTCGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTg                                                                                                                   <  4:691975/110‑1 (MQ=255)
                                                           tCGCGATTGTCTCGACGTTAAGCCCGAACTTTTTCACCATCGATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTa                                                                                                                                       >  3:699538/1‑87 (MQ=255)
                                                           tCGCGATTGTCTCGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATCTTCTCCAGCAAACCTCAGTGAACGCCATTa                                                                                                                                       <  4:699538/87‑1 (MQ=255)
                                                           tCGCGATTGTCTCGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTa                                                                                  <  3:459499/140‑1 (MQ=255)
                                                           tCGCGATTGTCTCGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTa                                                                                  >  4:459499/1‑140 (MQ=255)
                                                                aTTGTCTCGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCggg                                                                                                                            >  3:1321/1‑93 (MQ=255)
                                                                aTTGTCTCGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCggg                                                                                                                            <  4:1321/93‑1 (MQ=255)
                                                                  tGTCTCGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCAt                                                                                               <  3:611794/120‑1 (MQ=255)
                                                                  tGTCTCGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGgcg                                                                   >  3:1014920/1‑148 (MQ=255)
                                                                     ctcGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCg                                                                                                    >  4:179221/1‑112 (MQ=255)
                                                                     ctcGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCg                                                                                                    <  3:179221/112‑1 (MQ=255)
                                                                      tcGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGa                                                               >  4:717695/1‑148 (MQ=255)
                                                                         aCGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCggg                                                                                                                            <  3:96752/84‑1 (MQ=255)
                                                                             tAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCg                                                                                                    <  3:476982/104‑1 (MQ=255)
                                                                             tAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCg                                                                                                    >  4:476982/1‑104 (MQ=255)
                                                                             tAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGgcg                                                                   <  2:71593/137‑1 (MQ=255)
                                                                             tAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGgcg                                                                   >  1:71593/1‑137 (MQ=255)
                                                                                 cccGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCATGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGACCGGCGCAGtt                                                    >  3:1041316/1‑148 (MQ=255)
                                                                                  ccGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGACCGGCGCAGTtg                                                   <  4:1014920/148‑1 (MQ=255)
                                                                                  ccGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGACCGGCGCAGTtg                                                   >  4:326695/1‑148 (MQ=255)
                                                                                  ccGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGACCGGCGCAGTtg                                                   <  3:717695/148‑1 (MQ=255)
                                                                                   cGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGACCGCCATAATGGCGTTc                                                                                                                               >  1:512383/1‑71 (MQ=255)
                                                                                   cGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGt                                                                        >  1:35212/1‑126 (MQ=255)
                                                                                   cGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGt                                                                        <  2:35212/126‑1 (MQ=255)
                                                                                    gAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACt                                                                                          >  2:236309/1‑107 (MQ=255)
                                                                                    gAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACt                                                                                          <  1:236309/107‑1 (MQ=255)
                                                                                     aaCTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAgg                                                                                <  1:1112955/116‑1 (MQ=255)
                                                                                     aaCTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAgg                                                                                >  2:1112955/1‑116 (MQ=255)
                                                                                     aaCTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTg                                                                >  4:585181/1‑132 (MQ=255)
                                                                                     aaCTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTg                                                                <  3:585181/132‑1 (MQ=255)
                                                                                               ccATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGACCGGCGCAGTTGCTGCATCTAtcgt                                      >  3:496391/1‑148 (MQ=255)
                                                                                                  tCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTg                                                                         <  2:774974/110‑1 (MQ=255)
                                                                                                  tCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTg                                                                         >  1:774974/1‑110 (MQ=255)
                                                                                                     aTAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGa                                                                                            >  4:822739/1‑88 (MQ=255)
                                                                                                     aTAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGa                                                                                            <  3:822739/88‑1 (MQ=255)
                                                                                                      tAACTTATTCAATATTCTCCAGCAAACCTCAGTGACCGCCATAATGGCGTTc                                                                                                                               <  1:223709/52‑1 (MQ=37)
                                                                                                      tAACTTATTCAATATTCTCCAGCAAACCTCAGTGACCGCCATAATGGCGTTc                                                                                                                               >  2:223709/1‑52 (MQ=37)
                                                                                                      tAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTg                                                                >  3:1141603/1‑115 (MQ=255)
                                                                                                      tAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTg                                                                <  2:853582/115‑1 (MQ=255)
                                                                                                      tAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTg                                                                >  1:853582/1‑115 (MQ=255)
                                                                                                      tAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGACCGgc                                                          <  3:689756/121‑1 (MQ=255)
                                                                                                      tAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGACCGgc                                                          >  4:689756/1‑121 (MQ=255)
                                                                                                      tAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGACCGGCGCAGTTGCTGCATCTAt                                         >  4:125643/1‑138 (MQ=255)
                                                                                                        aCTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGACCGGCGCAGTTGCTGCATCTATCGTCGGCATTGa                             >  3:1013456/1‑148 (MQ=255)
                                                                                                        aCTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTAGCGCATCGATTTATCGGTAGGTTCTCTGTTGGCGCTGACCGGTGCAGTTGTTGCATCTATCGTCGGCATTGa                             >  3:954372/1‑148 (MQ=255)
                                                                                                         cTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGACCGGCGCAGTtgct                                                 >  3:348347/1‑127 (MQ=255)
                                                                                                           tATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTa                                                                                  <  4:385346/92‑1 (MQ=255)
                                                                                                             ttCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGACCGGCGCAGTTGCTGCATCTATCGTCGGCATTGAAGTCa                        <  3:326695/148‑1 (MQ=255)
                                                                                                              tCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTtctc                                                                           >  3:552948/1‑96 (MQ=255)
                                                                                                              tCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTtctc                                                                           <  4:552948/96‑1 (MQ=255)
                                                                                                                aaTATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGgcg                                                                   <  3:613658/102‑1 (MQ=255)
                                                                                                                aaTATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGgcg                                                                   >  4:613658/1‑102 (MQ=255)
                                                                                                                 atatTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCTACTTATCGGt                                                                                   <  3:749028/85‑1 (MQ=255)
                                                                                                                 atatTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATc                                                                                      >  2:418022/1‑82 (MQ=255)
                                                                                                                 atatTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATc                                                                                      <  1:418022/82‑1 (MQ=255)
                                                                                                                 atatTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGt                                                                                   >  4:749028/1‑85 (MQ=255)
                                                                                                                 atatTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTgg                                                                     <  3:1087478/99‑1 (MQ=255)
                                                                                                                 atatTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTgg                                                                     >  4:1087478/1‑99 (MQ=255)
                                                                                                                  taatctcCAGCAAACCTCAGTGAACGCCATGATGACGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCCCTGTTGGCGCTGACTGGCGCAGTTGCTGCATCTATCGTCGGCATTGAAGTCAATgcg                   <  4:954372/145‑1 (MQ=255)
                                                                                                                     tctcCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGtt                                                                       >  1:553276/1‑93 (MQ=255)
                                                                                                                     tctcCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGtt                                                                       <  2:553276/93‑1 (MQ=255)
                                                                                                                     tctcCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGACCGGCGCAGTTGCTGCATCTATCGTCGGCATTGAAGTCAATGCGCtg                <  4:496391/148‑1 (MQ=255)
                                                                                                                        ccAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGa                                                                                                               >  1:866512/1‑50 (MQ=255)
                                                                                                                          aGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTACCGGTAGGTTCTCTGTTGGCGCTGACCGGCGCAGTTGCTGCATCTATCGTCGGCATTGAAGTCAATGCGCTGGTGGc           >  4:615362/1‑148 (MQ=255)
                                                                                                                           gCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGt                                                                                   <  3:933663/75‑1 (MQ=255)
                                                                                                                           gCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGt                                                                                   >  4:933663/1‑75 (MQ=255)
                                                                                                                            cAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTtc                                                                             <  4:27905/80‑1 (MQ=255)
                                                                                                                            cAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTtc                                                                             >  3:27905/1‑80 (MQ=255)
                                                                                                                            cAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTGTCTGTTGGCGCTGACCGGCGCAGTTGCTGCATCTATCGTCGGCATTGaa                            >  4:912059/1‑129 (MQ=255)
                                                                                                                            cAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGACCGGCGCAGTTGCTGCATCTATCGTCGGCATTGaa                            <  3:912059/129‑1 (MQ=255)
                                                                                                                             aaaCCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGACCGGCGCAGTTGCTGCATCTATCGTCGGCATTGAAGTCAATGCGCTGGTGGCTGt        >  2:1087076/1‑148 (MQ=255)
                                                                                                                                  tCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTa                                                                                        <  1:293050/63‑1 (MQ=255)
                                                                                                                                  tCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTa                                                                                        >  2:293050/1‑63 (MQ=255)
                                                                                                                                  tCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGa                                                               >  4:642864/1‑88 (MQ=255)
                                                                                                                                  tCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGACCGGCGCAGTTGCTGCATCTATCGTCGGc                                  <  4:428018/117‑1 (MQ=255)
                                                                                                                                  tCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGACCGGCGCAGTTGCTGCATCTATCGTCGGc                                  >  3:428018/1‑117 (MQ=255)
                                                                                                                                  tCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGACCGGCGCAGTTGCTGCATCTATCGTCGGCATTGAAGTCAATGCGCtggtgg            >  2:497620/1‑139 (MQ=255)
                                                                                                                                  tCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGACCGGCGCAGTTGCTGCATCTATCGTCGGCATTGAAGTCAATGCGCtggtgg            >  2:682647/1‑139 (MQ=255)
                                                                                                                                  tCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGACCGGCGCAGTTGCTGCATCTATCGTCGGCATTGAAGTCAATGCGCtggtgg            <  1:682647/139‑1 (MQ=255)
                                                                                                                                  tCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGACCGGCGCAGTTGCTGCATCTATCGTCGGCATTGAAGTCAATGCGCtggtgg            <  1:497620/139‑1 (MQ=255)
                                                                                                                                  tCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGACCGGCGCAGTTGCTGCATCTATCGTCGGCATTGAAGTCAATGCGCTGGTGGCTGTCgctg   >  4:946687/1‑148 (MQ=255)
                                                                                                                                   cAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGACCGGCGCAGTTGCTGCATCTATCGTCGGCATTGAAGTCAATGCGCTGGTGGCTGTCgctgc  >  1:948501/1‑148 (MQ=255)
                                                                                                                                     gTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGACCGGCGCAGTTGCTGCATCTATCGTCgg                                   >  3:241405/1‑113 (MQ=255)
                                                                                                                                     gTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGACCGGCGCAGTTGCTGCATCTATCGTCgg                                   <  4:241405/113‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                             
TTATTTCACGAAAGCGTGGCTGATGGAGCAGAAATCGCTTATCGCTCTGCTGGTGCTGATCGCGATTGTCTCGACGTTAAGCCCGAACTTTTTCACCATCAATAACTTATTCAATATTCTCCAGCAAACCTCAGTGAACGCCATTATGGCGGTCGGGATGACGCTGGTGATCCTGACGTCGGGCATCGACTTATCGGTAGGTTCTCTGTTGGCGCTGACCGGCGCAGTTGCTGCATCTATCGTCGGCATTGAAGTCAATGCGCTGGTGGCTGTCGCTGC  >  NC_000913/3935317‑3935595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: