Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,981,102 T→A 9.8% V72V (GTT→GTA yifK → putative APC family amino acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,981,1020TA 9.8% 298.7 / 21.6 92V72V (GTT→GTAyifKputative APC family amino acid transporter
Reads supporting (aligned to +/- strand):  ref base T (41/42);  new base A (4/5);  total (45/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.80e-03

Only 100 of 102 total aligned reads displayed.

CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTGGGGATCTCTGAAATCACCGCCATTGGCGTTTATG  >  NC_000913/3980958‑3981229
                                                                                                                                                |                                                                                                                               
cACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggtaacc                                                                                                                              >  1:777305/1‑148 (MQ=255)
    aTTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccggtt                                                                                                                          >  2:844720/1‑148 (MQ=255)
      tGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTAACCGgttcg                                                                                                                        <  2:777305/148‑1 (MQ=255)
                       tGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCa                                                                                                       >  4:824341/1‑148 (MQ=255)
                         ggggCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGt                                                                                                                <  3:1005753/137‑1 (MQ=255)
                         ggggCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGt                                                                                                                >  4:1005753/1‑137 (MQ=255)
                            gccgccAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTGTATGCGCATCGtt                                                                                                  <  1:844720/148‑1 (MQ=255)
                              cgccAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATgcgc                                                                                                        <  1:231939/140‑1 (MQ=255)
                              cgccAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATgcgc                                                                                                        >  2:231939/1‑140 (MQ=255)
                               gccAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtata                                                                                               <  3:824341/148‑1 (MQ=255)
                                        cTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGt                                                                                                   <  2:110945/135‑1 (MQ=255)
                                        cTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGt                                                                                                   >  1:110945/1‑135 (MQ=255)
                                         tGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAg                                                                                           <  1:1114791/142‑1 (MQ=255)
                                         tGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAg                                                                                           >  2:1114791/1‑142 (MQ=255)
                                         tGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGtt                                                                                     >  3:695232/1‑148 (MQ=255)
                                          gAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccggtt                                                                                                                          <  4:847516/110‑1 (MQ=255)
                                          gAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccggtt                                                                                                                          >  3:847516/1‑110 (MQ=255)
                                          gAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTc                                                                                    >  2:804158/1‑148 (MQ=255)
                                             aTGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtata                                                                                               <  3:654069/134‑1 (MQ=255)
                                             aTGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtata                                                                                               >  4:654069/1‑134 (MQ=255)
                                                          tCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTAACCGgttcg                                                                                                                        >  4:1028202/1‑96 (MQ=255)
                                                          tCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTAACCGgttcg                                                                                                                        <  2:788223/96‑1 (MQ=255)
                                                          tCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTAACCGgttcg                                                                                                                        <  3:1028202/96‑1 (MQ=255)
                                                          tCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTAACCGgttcg                                                                                                                        >  1:788223/1‑96 (MQ=255)
                                                            cGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGcc                                                                  <  1:804158/148‑1 (MQ=255)
                                                                     ggCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgtt                                                                                                                      >  4:351420/1‑87 (MQ=255)
                                                                     ggCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgtt                                                                                                                      <  3:351420/87‑1 (MQ=255)
                                                                       ccTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccggtt                                                                                                                          >  3:132214/1‑81 (MQ=255)
                                                                       ccTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccggtt                                                                                                                          <  4:132214/81‑1 (MQ=255)
                                                                       ccTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTg                                                       <  4:695232/148‑1 (MQ=255)
                                                                         tataTCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGtt                                                                                     >  1:345064/1‑116 (MQ=255)
                                                                         tataTCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGtt                                                                                     <  2:345064/116‑1 (MQ=255)
                                                                           tatCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttc                                                                                                                         <  1:779211/78‑1 (MQ=255)
                                                                           tatCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttc                                                                                                                         >  2:779211/1‑78 (MQ=255)
                                                                            atcatcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGtt                                                                                                               <  2:818938/87‑1 (MQ=255)
                                                                            atcatcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGtt                                                                                                               >  1:818938/1‑87 (MQ=255)
                                                                             tcatcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggtta                                                                                                                                >  4:573410/1‑69 (MQ=255)
                                                                             tcatcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggtta                                                                                                                                <  3:573410/69‑1 (MQ=255)
                                                                              catcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCt                                                            >  3:950741/1‑136 (MQ=255)
                                                                               atcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtata                                                                                               <  3:127178/100‑1 (MQ=255)
                                                                               atcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtata                                                                                               >  4:127178/1‑100 (MQ=255)
                                                                                tcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTgg                                                      >  3:719650/1‑140 (MQ=255)
                                                                                tcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTgg                                                      <  4:719650/140‑1 (MQ=255)
                                                                                   ccGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTgg                                                                               >  3:677743/1‑112 (MQ=255)
                                                                                   ccGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTgg                                                                               >  1:1006837/1‑112 (MQ=255)
                                                                                   ccGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTgg                                                                               <  2:1006837/112‑1 (MQ=255)
                                                                                   ccGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTgg                                                                               <  4:677743/112‑1 (MQ=255)
                                                                                            tCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtat                                                                                                <  2:265353/86‑1 (MQ=255)
                                                                                            tCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtat                                                                                                >  1:265353/1‑86 (MQ=255)
                                                                                            tCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCttt                                                                                 <  4:30797/101‑1 (MQ=255)
                                                                                            tCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCttt                                                                                 >  3:30797/1‑101 (MQ=255)
                                                                                              gTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTa                                                  >  1:212226/1‑130 (MQ=255)
                                                                                              gTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTa                                                  <  2:212226/130‑1 (MQ=255)
                                                                                                cTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGtt                                                                                                               <  2:791856/67‑1 (MQ=255)
                                                                                                cTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGtt                                                                                                               >  1:791856/1‑67 (MQ=255)
                                                                                                cTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGt                                                                                                   >  4:947255/1‑79 (MQ=255)
                                                                                                cTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGt                                                                                                   <  3:947255/79‑1 (MQ=255)
                                                                                                  ttttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGtt                                                                                                               <  3:328351/65‑1 (MQ=255)
                                                                                                  ttttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGtt                                                                                                               >  4:328351/1‑65 (MQ=255)
                                                                                                  ttttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGtt                                                    <  3:913119/124‑1 (MQ=255)
                                                                                                  ttttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGtt                                                    >  4:913119/1‑124 (MQ=255)
                                                                                                  ttttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTAtg                                                <  4:1052551/128‑1 (MQ=255)
                                                                                                  ttttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGt                                     >  3:124596/1‑139 (MQ=255)
                                                                                                  ttttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGt                                     <  4:124596/139‑1 (MQ=255)
                                                                                                      catcatGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTAc                                                         <  3:891712/115‑1 (MQ=255)
                                                                                                      catcatGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTAc                                                         >  4:891712/1‑115 (MQ=255)
                                                                                                           tGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTgg                                                      <  1:65063/113‑1 (MQ=255)
                                                                                                           tGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTgg                                                      >  2:65063/1‑113 (MQ=255)
                                                                                                                   aTGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCtt                                                           <  1:602575/100‑1 (MQ=255)
                                                                                                                   aTGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCtt                                                           >  2:602575/1‑100 (MQ=255)
                                                                                                                    tGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGt                                     >  3:246990/1‑121 (MQ=255)
                                                                                                                    tGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGt                                     >  3:125707/1‑121 (MQ=255)
                                                                                                                    tGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGt                                     <  4:246990/121‑1 (MQ=255)
                                                                                                                    tGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGt                                     <  4:125707/121‑1 (MQ=255)
                                                                                                                        cGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGt                                                     >  1:1040234/1‑101 (MQ=255)
                                                                                                                        cGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGt                                                     <  2:1040234/101‑1 (MQ=255)
                                                                                                                          aaaTGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTg                                                                >  2:248445/1‑88 (MQ=255)
                                                                                                                          aaaTGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTg                                                                <  1:248445/88‑1 (MQ=255)
                                                                                                                              gttgttCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGcc                                                                  <  2:243396/82‑1 (MQ=255)
                                                                                                                              gttgttCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGcc                                                                  >  1:243396/1‑82 (MQ=255)
                                                                                                                                  ttCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCtt                                                                                  <  3:458655/62‑1 (MQ=255)
                                                                                                                                  ttCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCtt                                                                                  >  4:458655/1‑62 (MQ=255)
                                                                                                                                     aacGAACCGGTAACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCttt                                                                                 <  3:341539/58‑1 (MQ=38)
                                                                                                                                     aacGAACCGGTAACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCttt                                                                                 >  4:341539/3‑60 (MQ=38)
                                                                                                                                      tCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGtt                                                    >  1:292930/1‑88 (MQ=255)
                                                                                                                                      tCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGtt                                                    <  2:292930/88‑1 (MQ=255)
                                                                                                                                      tCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTGGGGATctct                           <  1:690398/113‑1 (MQ=255)
                                                                                                                                      tCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTGGGGATctct                           >  2:690398/1‑113 (MQ=255)
                                                                                                                                      tCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTGGGGATCTCTGAAATCa                    <  2:175655/120‑1 (MQ=255)
                                                                                                                                      tCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTGGGGATCTCTGAAATCa                    >  1:175655/1‑120 (MQ=255)
                                                                                                                                       cGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTta                                                                                                 <  2:598074/42‑1 (MQ=255)
                                                                                                                                       cGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTta                                                                                                 >  1:598074/1‑42 (MQ=255)
                                                                                                                                       cGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTta                                                                                                 >  3:713576/1‑42 (MQ=255)
                                                                                                                                       cGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTta                                                                                                 <  4:713576/42‑1 (MQ=255)
                                                                                                                                        gAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTGGGGATCTCTGAAATCACCGCCATTGGCGTTTATg  <  4:252912/136‑1 (MQ=255)
                                                                                                                                        gAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTGGGGATCTCTGAAATCACCGCCATTGGCGTTTATg  >  3:252912/1‑136 (MQ=255)
                                                                                                                                        gAACCGGTAACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTgg                                   >  4:401218/1‑103 (MQ=255)
                                                                                                                                        gAACCGGTAACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTgg                                   <  3:401218/103‑1 (MQ=255)
                                                                                                                                               ttaccggttCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCt                                                                 >  1:474778/1‑66 (MQ=255)
                                                                                                                                               ttaccggttCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCt                                                                 <  2:474778/66‑1 (MQ=255)
                                                                                                                                                |                                                                                                                               
CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTGGGGATCTCTGAAATCACCGCCATTGGCGTTTATG  >  NC_000913/3980958‑3981229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: