Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,981,376 A→G 5.4% M164V (ATG→GTG)  yifK → putative APC family amino acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,981,3760AG 5.4% 254.1 / 3.1 74M164V (ATG→GTG) yifKputative APC family amino acid transporter
Reads supporting (aligned to +/- strand):  ref base A (33/37);  new base G (2/2);  total (35/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

CCAGTTCTGGTTCCCGGAGATGGCGCAGTGGATACCCGCATTGATCGCAGTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCGGTTTCTTTGCGGGTGGCTGGAAAGGGTTCCTGACCGCTCTGTGTATTGTGGTGGCG  >  NC_000913/3981231‑3981519
                                                                                                                                                 |                                                                                                                                               
ccAGTTCTGGTTCCCGGAGATGGCGCAGTGGATACCCGCATTGATCGCAGTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGtgatg                                                                                                                                               >  2:1072554/1‑148 (MQ=255)
ccAGTTCTGGTTCCCGGAGATGGCGCAGTGGATACCCGCATTGATCGCAGTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGtgatg                                                                                                                                               >  1:103464/1‑148 (MQ=255)
ccAGTTCTGGTTCCCGGAGATGGCGCAGTGGATACCCGCATTGATCGCAGTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGtga                                                                                                                                                 >  3:1104179/1‑146 (MQ=255)
ccAGTTCTGGTTCCCGGAGATGGCGCAGTGGATACCCGCATTGATCGCAGTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGtga                                                                                                                                                 <  4:1104179/146‑1 (MQ=255)
     tCTGGTTCCCGGAGATGGCGCAGTGGATACCCGCATTGATCGCAGTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGt                                                                                                                                          >  3:389665/1‑148 (MQ=255)
       tGGTTCCCGGAGATGGCGCAGTGGATACCCGCATTGATCGCAGTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCa                                                                                                                                        >  2:219750/1‑148 (MQ=255)
       tGGTTCCCGGAGATGGCGCAGTGGATACCCGCATTGATCGCAGTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCa                                                                                                                                        >  1:923008/1‑148 (MQ=255)
        ggTTCCCGGAGATGGCGCAGTGGATACCCGCATTGATCGCAGTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCAt                                                                                                                                       <  2:849795/148‑1 (MQ=255)
          ttCCCGGAGATGGCGCAGTGGATACCCGCATTGATCGCAGTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTg                                                                                                                                     >  4:490119/1‑148 (MQ=255)
             ccGGAGATGGCGCAGTGGATACCCGCATTGATCGCAGTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCa                                                                                                                                        >  3:154009/1‑142 (MQ=255)
             ccGGAGATGGCGCAGTGGATACCCGCATTGATCGCAGTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCa                                                                                                                                        <  4:154009/142‑1 (MQ=255)
              cGGAGATGGCGCAGTGGATACCCGCATTGATCGCAGTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCt                                                                                                                                 >  3:712127/1‑148 (MQ=255)
               ggAGATGGCGCAGTGGATACCCGCATTGATCGCAGTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTg                                                                                                                                >  4:436807/1‑148 (MQ=255)
                    tGGCGCAGTGGATACCCGCATTGATCGCAGTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGt                                                                                                                           <  1:1072554/148‑1 (MQ=255)
                     ggCGCAGTGGATACCCGCATTGATCGCAGTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTg                                                                                                                          <  4:675257/148‑1 (MQ=255)
                         cAGTGGATACCCGCATTGATCGCAGTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGAttt                                                                                                                      >  2:993083/1‑148 (MQ=255)
                          aGTGGATACCCGCATTGATCGCAGTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGAtttt                                                                                                                     <  2:103464/148‑1 (MQ=255)
                            tGGATACCCGCATTGATCGCAGTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTct                                                                                                                   <  3:490119/148‑1 (MQ=255)
                                    cGCATTGATCGCAGTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCa                                                                                                                                        <  2:883549/119‑1 (MQ=255)
                                    cGCATTGATCGCAGTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCa                                                                                                                                        >  1:883549/1‑119 (MQ=255)
                                    cGCATTGATCGCAGTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCAt                                                                                                                                       <  3:620851/120‑1 (MQ=255)
                                    cGCATTGATCGCAGTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCAt                                                                                                                                       >  4:620851/1‑120 (MQ=255)
                                    cGCATTGATCGCAGTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTctttggcttt                                                                                                           <  1:219750/148‑1 (MQ=255)
                                           aTCGCAGTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATg                                                                                                    >  3:738176/1‑148 (MQ=255)
                                                 gTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCCAAGTCAACACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCGATGGCGGGc                                                                                              <  4:738176/148‑1 (MQ=255)
                                                    gcgcTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGt                                                                                           <  4:715342/148‑1 (MQ=255)
                                                     cgcTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTg                                                                                                                                >  2:622339/1‑110 (MQ=255)
                                                     cgcTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTg                                                                                                                                <  1:622339/110‑1 (MQ=255)
                                                         ggtggCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGAtt                                                                                      >  1:584055/1‑148 (MQ=255)
                                                            ggCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGt                                                                                   <  3:436807/148‑1 (MQ=255)
                                                                   gCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCa                                                                            <  1:993083/148‑1 (MQ=255)
                                                                   gCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCa                                                                            >  1:685811/1‑148 (MQ=255)
                                                                      aaTCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCAtt                                                                                                                                      >  2:856183/1‑87 (MQ=255)
                                                                      aaTCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCAtt                                                                                                                                      <  1:856183/87‑1 (MQ=255)
                                                                         cTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTca                                                                      >  1:564739/1‑148 (MQ=255)
                                                                          tGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTcac                                                                     <  4:712127/148‑1 (MQ=255)
                                                                                cggtgcggtTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCa                                                               <  2:685811/148‑1 (MQ=255)
                                                                                        ttGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCGGtt                                                       <  2:360552/148‑1 (MQ=255)
                                                                                         tGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCGGttt                                                      <  2:564739/148‑1 (MQ=255)
                                                                                           tACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCGGTTTCt                                                    >  1:374777/1‑148 (MQ=255)
                                                                                              ggCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTct                                                                                                                   <  4:896274/82‑1 (MQ=255)
                                                                                              ggCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTct                                                                                                                   >  3:896274/1‑82 (MQ=255)
                                                                                              ggCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATgg                                                            <  2:195093/137‑1 (MQ=255)
                                                                                              ggCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATgg                                                            >  1:195093/1‑137 (MQ=255)
                                                                                               gCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTgg                                                                                    >  2:780765/1‑112 (MQ=255)
                                                                                               gCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTgg                                                                                    <  1:780765/112‑1 (MQ=255)
                                                                                                 gAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGAt                                                                                       >  1:312910/1‑107 (MQ=255)
                                                                                                 gAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGAt                                                                                       <  2:312910/107‑1 (MQ=255)
                                                                                                    aTCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCgg                                                         <  4:471502/134‑1 (MQ=255)
                                                                                                    aTCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCgg                                                         >  3:471502/1‑134 (MQ=255)
                                                                                                        aGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCGGtt                                                       >  1:540537/1‑132 (MQ=255)
                                                                                                        aGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCGGtt                                                       <  2:540537/132‑1 (MQ=255)
                                                                                                              ggTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTa                                                                               <  1:200140/102‑1 (MQ=255)
                                                                                                              ggTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTa                                                                               >  2:200140/1‑102 (MQ=255)
                                                                                                                ttCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGAt                                                                                                                        <  2:5957/59‑1 (MQ=255)
                                                                                                                ttCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGAt                                                                                                                        >  1:5957/1‑59 (MQ=255)
                                                                                                                      atgatCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGtttt                                                                                <  3:492607/93‑1 (MQ=255)
                                                                                                                      atgatCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGtttt                                                                                >  4:492607/1‑93 (MQ=255)
                                                                                                                        gatCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCGGTTTCTTTGCGGGTGGCTGGaa                                    >  2:622674/1‑135 (MQ=255)
                                                                                                                        gatCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCGGTTTCTTTGCGGGTGGCTGGaa                                    <  1:622674/135‑1 (MQ=255)
                                                                                                                          tCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGtttt                                                                                <  3:749164/89‑1 (MQ=255)
                                                                                                                          tCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGtttt                                                                                >  4:749164/1‑89 (MQ=255)
                                                                                                                          tCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCgg                                                         >  4:609628/1‑112 (MQ=255)
                                                                                                                          tCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGGGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCgg                                                         <  3:609628/112‑1 (MQ=255)
                                                                                                                              aGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCGGTTTCTTTGCGGGTGGCTGGAAAGGGTTCCTGACCGCTCtg                 <  2:374777/148‑1 (MQ=255)
                                                                                                                                 catcacGATAATCGTGGTGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACagag                                                                 >  1:743101/4‑97 (MQ=255)
                                                                                                                                 catcacGATAATCGTGGTGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACagag                                                                 <  2:743101/94‑1 (MQ=255)
                                                                                                                                 caccacGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCGGTTTCTTTGCGGGTGGCTGGAAAGGGTTCCTGACCGCTCTgtgt              <  4:389665/148‑1 (MQ=255)
                                                                                                                                   tcacGATAATCGTGGTGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCGGTTTCTTTGCGGGTGGCTGGAAAg                                  >  1:973807/2‑126 (MQ=255)
                                                                                                                                   tcacGATAATCGTGGTGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCGGTTTCTTTGCGGGTGGCTGGAAAg                                  <  2:973807/125‑1 (MQ=255)
                                                                                                                                    cacGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCGGTTTCTTTGCGGGTGGCTGGAAAGGGTTcc                            <  1:544964/131‑1 (MQ=255)
                                                                                                                                    cacGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCGGTTTCTTTGCGGGTGGCTGGAAAGGGTTcc                            >  2:544964/1‑131 (MQ=255)
                                                                                                                                      cGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCGGTTTCTTTGCGGGTGGCTGGaa                                    >  1:214244/1‑121 (MQ=255)
                                                                                                                                      cGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCGGTTTCTTTGCGGGTGGCTGGaa                                    <  2:214244/121‑1 (MQ=255)
                                                                                                                                        aTTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCGGTTTCTTTGCGGGTGGCTGGAAAGGGTTCCTGACCGCTCTGTGTATTgtgg       >  1:467967/1‑148 (MQ=255)
                                                                                                                                           aTCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCGGTTTCTTTGCGGGTGGCTGGAAAGGGTTCCTGACCGCTCtgtg               >  1:844745/1‑137 (MQ=255)
                                                                                                                                           aTCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCGGTTTCTTTGCGGGTGGCTGGAAAGGGTTCCTGACCGCTCtgtg               <  2:844745/137‑1 (MQ=255)
                                                                                                                                             cGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCGGTTTCTTTGCGGGTGGCTGGAAAGGGTTCCTGACCGCTCTGTGTATTGTGGTGGCg  <  2:467967/148‑1 (MQ=255)
                                                                                                                                                gatgatTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCGGTTTCTTTGCGGGTGGCTGGAAAGGGTTCCTGACCGCTCtg                 >  1:414289/1‑130 (MQ=255)
                                                                                                                                                gatgatTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCGGTTTCTTTGCGGGTGGCTGGAAAGGGTTCCTGACCGCTCtg                 <  3:86997/130‑1 (MQ=255)
                                                                                                                                                gatgatTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCGGTTTCTTTGCGGGTGGCTGGAAAGGGTTCCTGACCGCTCtg                 >  4:86997/1‑130 (MQ=255)
                                                                                                                                                gatgatTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCGGTTTCTTTGCGGGTGGCTGGAAAGGGTTCCTGACCGCTCtg                 <  2:414289/130‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CCAGTTCTGGTTCCCGGAGATGGCGCAGTGGATACCCGCATTGATCGCAGTGGCGCTGGTGGCGTTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACGATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAATCTCACAGAGCATGGCGGTTTCTTTGCGGGTGGCTGGAAAGGGTTCCTGACCGCTCTGTGTATTGTGGTGGCG  >  NC_000913/3981231‑3981519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: