Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,080,616 C→A 7.9% G205C (GGC→TGC)  fdhE ← formate dehydrogenase formation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,080,6160CA 7.9% 485.5 / 25.5 139G205C (GGC→TGC) fdhEformate dehydrogenase formation protein
Reads supporting (aligned to +/- strand):  ref base C (65/63);  new base A (5/6);  total (70/69)
Fisher's exact test for biased strand distribution p-value = 7.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.93e-01

Only 100 of 139 total aligned reads displayed.

TTCGGCTTTAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATAAACGGCGCT  >  NC_000913/4080470‑4080761
                                                                                                                                                  |                                                                                                                                                 
ttCGGCTTTAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCa                                                                                                                                                  >  2:1119633/1‑148 (MQ=255)
 tCGGCTTTAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCaa                                                                                                                                                 <  2:500709/148‑1 (MQ=255)
 tCGGCTTTAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCaa                                                                                                                                                 <  1:1119633/148‑1 (MQ=255)
     cTTTAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTg                                                                                                                                             >  1:1039389/1‑148 (MQ=255)
     cTTTAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTg                                                                                                                                             >  4:206294/1‑148 (MQ=255)
     cTTTAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTg                                                                                                                                             <  2:1039389/148‑1 (MQ=255)
       ttAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCa                                                                                                                                           >  4:933684/1‑148 (MQ=255)
          aTCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCAAATTGGCACCa                                                                                                                                        <  1:1033112/148‑1 (MQ=255)
              cgGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCt                                                                                                                                    <  3:933684/148‑1 (MQ=255)
                     ttCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACAcc                                                                                                                             >  3:488410/1‑148 (MQ=255)
                          cATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCAt                                                                                                                        <  2:398263/148‑1 (MQ=255)
                            tCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAg                                                                                                                      >  4:919727/1‑148 (MQ=255)
                            tCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAg                                                                                                                      >  3:447630/1‑148 (MQ=255)
                             ccAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCAAATTGGCACCa                                                                                                                                        >  3:931166/1‑129 (MQ=255)
                             ccAGCGACCAGTAATGCAGTTTGCCGCACTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCAAATTGGCACCa                                                                                                                                        <  4:931166/129‑1 (MQ=255)
                              cAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGaa                                                                                                                    <  4:447630/148‑1 (MQ=255)
                               aGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAAc                                                                                                                   >  3:548733/1‑148 (MQ=255)
                               aGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAAc                                                                                                                   >  3:1083421/1‑148 (MQ=255)
                                gCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAAcc                                                                                                                  <  3:206294/148‑1 (MQ=255)
                                    ccAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACaa                                                                                                              <  4:548733/148‑1 (MQ=255)
                                       gTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCCCACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAAcc                                                                                                           <  3:322395/148‑1 (MQ=255)
                                       gTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAAcc                                                                                                           >  4:322395/1‑148 (MQ=255)
                                        tAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCg                                                                                                          <  4:488410/148‑1 (MQ=255)
                                            gCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCa                                                                                                      >  4:1094839/1‑148 (MQ=255)
                                             cAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTgg                                                                                                                                  >  4:336617/1‑119 (MQ=255)
                                              aGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAAtt                                                                                                                                               <  2:1066934/105‑1 (MQ=255)
                                                       ctctGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTg                                                                                                >  1:344369/1‑143 (MQ=255)
                                                       ctctGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTg                                                                                                <  2:344369/143‑1 (MQ=255)
                                                        tctGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACca                                                                                                                 >  2:579617/1‑125 (MQ=255)
                                                              cGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAAtt                                                                                                                                               >  1:1019872/1‑89 (MQ=255)
                                                                        gcATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCa                                                                                                                                        >  2:863874/1‑86 (MQ=255)
                                                                        gcATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGaa                                                                                                                    <  4:791426/106‑1 (MQ=255)
                                                                            tttACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTg                                                                                                <  2:1125490/122‑1 (MQ=255)
                                                                            tttACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTg                                                                                                >  3:555038/1‑122 (MQ=255)
                                                                            tttACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTg                                                                                                <  4:555038/122‑1 (MQ=255)
                                                                            tttACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCggg                                                                      >  1:1102641/1‑148 (MQ=255)
                                                                              tACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCAAATTGGCACCactca                                                                                                                                    >  3:628882/1‑80 (MQ=39)
                                                                                cgcgCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGc                                                                                                                          <  3:818259/92‑1 (MQ=255)
                                                                                cgcgCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGc                                                                                                                          >  4:818259/1‑92 (MQ=255)
                                                                                cgcgCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTg                                                                                          <  1:441416/124‑1 (MQ=255)
                                                                                cgcgCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTg                                                                                          >  2:441416/1‑124 (MQ=255)
                                                                                      cTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAat                                                                                                    <  4:1019124/108‑1 (MQ=255)
                                                                                       tACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTAt                                                                                <  4:653404/127‑1 (MQ=255)
                                                                                       tACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTAt                                                                                >  3:653404/1‑127 (MQ=255)
                                                                                                ttCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCgggg                                                           >  1:899230/1‑139 (MQ=255)
                                                                                                 tCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATa                                                                                                                       <  4:1113446/78‑1 (MQ=255)
                                                                                                 tCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATa                                                                                                                       >  3:1113446/1‑78 (MQ=255)
                                                                                                 tCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCgg                                                                                                         <  4:97326/92‑1 (MQ=255)
                                                                                                 tCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGAGGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTgg                                                 >  3:982713/1‑148 (MQ=255)
                                                                                                  cAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTc                                                                              >  2:250293/1‑118 (MQ=255)
                                                                                                          cacaGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTg                                                  >  1:615504/1‑138 (MQ=255)
                                                                                                          cacaGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTgcg                                        >  1:351780/1‑148 (MQ=255)
                                                                                                          cacaGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTgcg                                        >  3:1073007/1‑148 (MQ=255)
                                                                                                            caGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGa                                                          <  3:645512/128‑1 (MQ=255)
                                                                                                            caGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGa                                                          >  4:645512/1‑128 (MQ=255)
                                                                                                              ggTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCgg                                                                                                         >  4:186389/1‑79 (MQ=255)
                                                                                                              ggTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCgg                                                                                                         <  3:186389/79‑1 (MQ=255)
                                                                                                                ttGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCt                                                                                                                                    >  3:824452/1‑50 (MQ=255)
                                                                                                                ttGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGt                                  >  3:680249/1‑148 (MQ=255)
                                                                                                                ttGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGt                                  >  2:653527/1‑148 (MQ=255)
                                                                                                                 tgcagtgcagGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGt                                                                                            <  2:1085701/89‑1 (MQ=255)
                                                                                                                 tgcagtgcagGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGt                                                                                            >  1:1085701/1‑89 (MQ=255)
                                                                                                                 tgcagtgcagGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTa                                 >  4:617476/1‑148 (MQ=255)
                                                                                                                     gtgcagGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGc                             <  4:1073007/148‑1 (MQ=255)
                                                                                                                      tgcagGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGt                                                                                  <  3:622019/94‑1 (MQ=255)
                                                                                                                      tgcagGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGt                                                                                  >  4:622019/1‑94 (MQ=255)
                                                                                                                      tgcagGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCgggg                                                           >  1:988421/1‑117 (MQ=255)
                                                                                                                      tgcagGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCgggg                                                           <  2:988421/117‑1 (MQ=255)
                                                                                                                       gcagGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTg                                          <  2:68492/133‑1 (MQ=255)
                                                                                                                       gcagGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTg                                          >  1:68492/1‑133 (MQ=255)
                                                                                                                            tAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGa                                                                                               >  3:42978/1‑75 (MQ=255)
                                                                                                                            tAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAg                                                      <  1:130640/116‑1 (MQ=255)
                                                                                                                            tAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGAc                          <  3:1074852/144‑1 (MQ=255)
                                                                                                                            tAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCAGCGCCCAGTAGAGCGAc                          >  4:1074852/1‑144 (MQ=255)
                                                                                                                              aCGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTg                                                                                          >  2:8706/1‑78 (MQ=255)
                                                                                                                              aCGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTg                                                                                          <  1:8706/78‑1 (MQ=255)
                                                                                                                                  aGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGccc                >  2:450367/1‑148 (MQ=255)
                                                                                                                                    ccccTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAatat                                                                                                  >  4:184722/1‑64 (MQ=255)
                                                                                                                                    ccccTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAatat                                                                                                  <  3:184722/64‑1 (MQ=255)
                                                                                                                                      ccTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTg                                                                                                >  3:565244/1‑64 (MQ=255)
                                                                                                                                      ccTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAg                                                      >  4:634680/1‑106 (MQ=255)
                                                                                                                                        tGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTgcg                                        >  1:58359/1‑118 (MQ=255)
                                                                                                                                        tGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTgcg                                        <  2:58359/118‑1 (MQ=255)
                                                                                                                                        tGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATaa          <  4:556558/148‑1 (MQ=255)
                                                                                                                                           atggtgCAAATTGGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTg                                          <  1:648085/112‑1 (MQ=255)
                                                                                                                                           atggtgCAAATTGGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTg                                          <  3:310124/112‑1 (MQ=255)
                                                                                                                                           atggtgCAAATTGGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTg                                          >  4:310124/2‑113 (MQ=255)
                                                                                                                                           atggtgCAAATTGGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTg                                          >  2:648085/2‑113 (MQ=255)
                                                                                                                                            tggtgCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTag                                <  3:9790/122‑1 (MQ=255)
                                                                                                                                            tggtgCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTag                                >  4:9790/1‑122 (MQ=255)
                                                                                                                                            tggtgCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAg                        <  3:1003364/130‑1 (MQ=255)
                                                                                                                                            tggtgCAAATTGGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCg                                                              <  1:544661/92‑1 (MQ=255)
                                                                                                                                             ggtgCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAg                                                                            <  1:769762/77‑1 (MQ=255)
                                                                                                                                             ggtgCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAg                                                                            >  2:769762/1‑77 (MQ=255)
                                                                                                                                                gCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGTCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTg                                          <  3:961541/108‑1 (MQ=255)
                                                                                                                                                gCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGAt                                                    >  2:198506/1‑98 (MQ=255)
                                                                                                                                                gCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGAt                                                    <  1:198506/98‑1 (MQ=255)
                                                                                                                                                gCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTaga                               >  2:377903/1‑119 (MQ=255)
                                                                                                                                                gCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTaga                               <  1:377903/119‑1 (MQ=255)
                                                                                                                                                gCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATAAACGGCGCt  >  3:1102884/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TTCGGCTTTAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATAAACGGCGCT  >  NC_000913/4080470‑4080761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: