Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,148,049 C→G 6.5% G90A (GGC→GCC)  yijO ← AraC family putative transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,148,0490CG 6.5% 380.5 / 15.0 107G90A (GGC→GCC) yijOAraC family putative transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (51/49);  new base G (4/3);  total (55/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.48e-02

Only 100 of 115 total aligned reads displayed.

TGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAGCGCCGGTATCAACAAACTCACCTTCCAGCACAATTTCCAG  >  NC_000913/4147914‑4148191
                                                                                                                                       |                                                                                                                                              
tgcatttgcattTCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGc                                                                                                                                    >  1:143703/1‑148 (MQ=255)
           ttCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGcc                                                                                                                                               >  2:743491/1‑126 (MQ=255)
           ttCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCaa                                                                                                                                             <  3:1060446/128‑1 (MQ=255)
           ttCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGgtagta                                                                                                                         >  4:970152/1‑148 (MQ=255)
           ttCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGgtagt                                                                                                                          >  3:195437/1‑147 (MQ=255)
           ttCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGgtagt                                                                                                                          <  4:195437/147‑1 (MQ=255)
           ttCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTGTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGcc                                                                                                                                               <  1:743491/126‑1 (MQ=255)
           ttCATTGAGCGTTTGTAGCAGAAAAGAACCTATGAGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGgtagt                                                                                                                          >  3:754719/1‑147 (MQ=255)
           ttCATTGAGCGTTTGTAGCAGAAAAGAACCTATGAGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGgtagt                                                                                                                          <  4:754719/147‑1 (MQ=255)
              aTTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCg                                                                                                                      <  3:970152/148‑1 (MQ=255)
              aTTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCg                                                                                                                      >  2:104220/1‑148 (MQ=255)
               ttGAGCGTTTGTAGCAGAAAAGAACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCgg                                                                                                                     <  4:1015418/148‑1 (MQ=255)
                tGAGCGTTTGTAGCAGAAAAGAACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGgta                                                                                                                            <  1:187700/140‑1 (MQ=255)
                tGAGCGTTTGTAGCAGAAAAGAACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGgta                                                                                                                            >  2:187700/1‑140 (MQ=255)
                       ttGTAGCAGAAAAGAACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTaaa                                                                                                                                >  4:340376/1‑129 (MQ=255)
                       ttGTAGCAGAAAAGAACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTaaa                                                                                                                                <  3:340376/129‑1 (MQ=255)
                            gCAGAAAAGAACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATtg                                                                                                        <  2:143703/148‑1 (MQ=255)
                             cAGAAAAGAACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAAGAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATtgt                                                                                                       <  3:77111/148‑1 (MQ=255)
                             cAGAAAAGAACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTACCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAAGAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATtgt                                                                                                       >  4:77111/1‑148 (MQ=255)
                               gAAAAGAACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCa                                                                                                                                       <  2:172949/114‑1 (MQ=255)
                               gAAAAGAACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATtgtg                                                                                                      <  2:564776/147‑1 (MQ=255)
                               gAAAAGAACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATtgtg                                                                                                      >  1:564776/1‑147 (MQ=255)
                                 aaaGAACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGGCAAACAGCaa                                                                                                                                      <  4:1122435/113‑2 (MQ=255)
                                         tATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAAc                                                                                                                                           >  1:797356/1‑100 (MQ=255)
                                         tATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAAc                                                                                                                                           <  2:797356/100‑1 (MQ=255)
                                          aTGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAg                                                                                           >  3:74853/1‑147 (MQ=255)
                                          aTGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAg                                                                                           <  4:74853/147‑1 (MQ=255)
                                          aTGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGc                                                                                          >  1:142330/1‑148 (MQ=255)
                                                   ccGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACcagcag                                                                                 <  2:142330/148‑1 (MQ=255)
                                                             cGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAg                                                                       <  1:104220/148‑1 (MQ=255)
                                                               aCGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATtg                                                                                                        <  3:480029/113‑1 (MQ=255)
                                                               aCGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAAAAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATtg                                                                                                        >  4:480029/1‑113 (MQ=255)
                                                                  tgtTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCg                                                                                                                      <  1:58157/96‑1 (MQ=255)
                                                                     tGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCgg                                                                                                                     <  3:576187/94‑1 (MQ=255)
                                                                     tGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCgg                                                                                                                     >  4:576187/1‑94 (MQ=255)
                                                                     tGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGc                                                               >  1:766170/1‑148 (MQ=255)
                                                                         tCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCggg                                                           <  2:766170/148‑1 (MQ=255)
                                                                            ccAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCAt                                                                                                          <  4:602859/98‑1 (MQ=255)
                                                                             cAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGgta                                                                                                                            >  2:537711/1‑79 (MQ=255)
                                                                             cAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGgta                                                                                                                            <  1:537711/79‑1 (MQ=255)
                                                                                  tttGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGaa                                                                              <  3:407564/120‑1 (MQ=255)
                                                                                  tttGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGaa                                                                              >  4:407564/1‑120 (MQ=255)
                                                                                     gATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATtg                                                                                                        >  4:520193/1‑91 (MQ=255)
                                                                                     gATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATtg                                                                                                        <  3:520193/91‑1 (MQ=255)
                                                                                        atTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATtg                                                                                                        <  3:995206/88‑1 (MQ=255)
                                                                                        atTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTAc                                                                         <  1:444151/119‑1 (MQ=255)
                                                                                        atTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTAc                                                                         >  2:444151/1‑119 (MQ=255)
                                                                                        atTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGcac                                                                    <  1:913779/124‑1 (MQ=255)
                                                                                        atTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGcac                                                                    >  2:913779/1‑124 (MQ=255)
                                                                                          tGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTa                                                      >  1:127110/1‑136 (MQ=255)
                                                                                          tGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTa                                                      <  2:127110/136‑1 (MQ=255)
                                                                                             ttGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAgcgc                                       >  3:746070/1‑148 (MQ=255)
                                                                                             ttGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAgcg                                        >  2:697502/1‑147 (MQ=255)
                                                                                             ttGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAgcg                                        <  1:697502/147‑1 (MQ=255)
                                                                                                ccATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAGCGCCgg                                    >  4:447898/1‑148 (MQ=255)
                                                                                                 cATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACg                                                   <  3:1018567/132‑1 (MQ=255)
                                                                                                 cATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACg                                                   >  4:1018567/1‑132 (MQ=255)
                                                                                                 cATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAGCGCCGGt                                   >  2:1015130/1‑148 (MQ=255)
                                                                                                  aTCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGGCAAACAGCa                                                                                                                                       <  3:539024/47‑1 (MQ=255)
                                                                                                  aTCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATtg                                                                                                        >  4:75777/1‑78 (MQ=255)
                                                                                                   tCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCt                                              <  4:453998/135‑1 (MQ=255)
                                                                                                   tCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAGCGCCGGTAt                                 <  4:746070/148‑1 (MQ=255)
                                                                                                    cccATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCgg                                                                                                                     <  2:900381/63‑1 (MQ=255)
                                                                                                     ccATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAGCGCCGGTATca                               >  1:780942/1‑148 (MQ=255)
                                                                                                      cATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCTGGGAACTAACGTTGCTCCAGCGcc                                      <  4:148598/140‑1 (MQ=255)
                                                                                                      cATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAGCGcc                                      >  3:148598/1‑140 (MQ=255)
                                                                                                        ttGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAGCGCCGGTATcaaca                            >  3:875423/1‑148 (MQ=255)
                                                                                                         tGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCtt                                                                                                               <  4:1101457/64‑1 (MQ=255)
                                                                                                         tGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCtt                                                                                                               >  3:1101457/1‑64 (MQ=255)
                                                                                                         tGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAGCGCCGGTATcaacaa                           <  1:1015130/148‑1 (MQ=255)
                                                                                                           cACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGt                                                                           >  3:44379/1‑98 (MQ=255)
                                                                                                           cACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGt                                                                           <  4:44379/98‑1 (MQ=255)
                                                                                                              gacgCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGca                                                                     <  3:1046355/101‑1 (MQ=255)
                                                                                                              gacgCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGca                                                                     >  4:1046355/1‑101 (MQ=255)
                                                                                                                   tGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAGCGCCgt                                    <  4:561216/129‑2 (MQ=255)
                                                                                                                   tGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAGCGCCgg                                    >  3:561216/1‑129 (MQ=255)
                                                                                                                    gAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAGCGCCGGTATcaacaa                           >  3:338015/1‑137 (MQ=255)
                                                                                                                    gAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAGCGCCGGTATCAACaaa                          >  1:59154/1‑138 (MQ=255)
                                                                                                                    gAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAGCGCCGGTATCAACaaa                          <  2:59154/138‑1 (MQ=255)
                                                                                                                      aGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTACTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAGCGCCGGTATCAACaaa                          <  2:681729/136‑1 (MQ=255)
                                                                                                                       gCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTa                                                      <  3:755957/107‑1 (MQ=255)
                                                                                                                       gCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTa                                                      >  4:755957/1‑107 (MQ=255)
                                                                                                                        ccGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAGCGCCGGTATcaacaa                           <  1:152457/133‑1 (MQ=255)
                                                                                                                             ttGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAGCGCCGGTATCAACAAACTCa                      <  4:601032/133‑1 (MQ=255)
                                                                                                                             ttGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAGCGCCGGTATCAACAAACTCa                      >  3:601032/1‑133 (MQ=255)
                                                                                                                               ggtGTTTGGCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAGCGCCGGTATCAACAAACTCACCTTc                 >  3:755216/3‑136 (MQ=255)
                                                                                                                               gCTGTTTGGCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAGCGCCGGTATCAACAAACTCACCTTc                 <  4:755216/136‑1 (MQ=255)
                                                                                                                                 tGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAGCGCCGGTATCAACAAACTCACCTTCCAGCACaa         <  3:754536/142‑1 (MQ=255)
                                                                                                                                  gTTTGGCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGGAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAGCGCCGGTATCAACAAACTCACCTTCCAGCACAATTTCCAg  >  2:333477/1‑148 (MQ=255)
                                                                                                                                  gTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGa                                                                               >  2:416995/1‑71 (MQ=255)
                                                                                                                                  gTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGa                                                                               <  1:416995/71‑1 (MQ=255)
                                                                                                                                    ttGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTcc                                                                                             <  3:211626/55‑1 (MQ=255)
                                                                                                                                    ttGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTcc                                                                                             >  4:211626/1‑55 (MQ=255)
                                                                                                                                      gCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAGCGCCGGTATCAACAAACTCACCTTc                 >  4:733270/1‑129 (MQ=255)
                                                                                                                                      gCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAGCGCCGGTATCAACAAACTCACCTTc                 <  3:733270/129‑1 (MQ=255)
                                                                                                                                       ccAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTcc                                                                                             <  2:118978/52‑1 (MQ=255)
                                                                                                                                       ccAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTcc                                                                                             >  1:118978/1‑52 (MQ=255)
                                                                                                                                       ccAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGccacc                                                                                      >  3:111818/1‑59 (MQ=255)
                                                                                                                                       ccAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGccacc                                                                                      <  4:111818/59‑1 (MQ=255)
                                                                                                                                       ccAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAGCGcc                                      >  1:873049/1‑107 (MQ=255)
                                                                                                                                       |                                                                                                                                              
TGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTCCAGCGCCGGTATCAACAAACTCACCTTCCAGCACAATTTCCAG  >  NC_000913/4147914‑4148191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: