Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,220,272 | T→C | 8.0% | D559D (GAT→GAC) | aceK → | isocitrate dehydrogenase kinase/phosphatase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,220,272 | 0 | T | C | 8.0% | 246.1 / 7.6 | 75 | D559D (GAT→GAC) | aceK | isocitrate dehydrogenase kinase/phosphatase |
Reads supporting (aligned to +/- strand): ref base T (31/38); new base C (3/3); total (34/41) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 |
GATGTTTTCCCGGAAGAGTTTCGCCACTGGCTATGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCTTCCATATAATTTTTCTCCGCAATGTATCGAGGGTTATCCGTAAAGCCAAAGCTTTC > NC_000913/4220129‑4220396 | gATGTTTTCCCGGAAGAGTTTCGCCACTGGCTATGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGttt < 1:27560/148‑1 (MQ=255) aTGTTTTCCCGGAAGAGTTTCGCCACTGGCTATGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTa < 2:14816/148‑1 (MQ=255) tGTTTTCCCGGAAGAGTTTCGCCACTGGCTATGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTAt < 4:480072/148‑1 (MQ=255) gTTTTCCCGGAAGAGTTTCGCCACTGGCTATGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATg < 2:575513/148‑1 (MQ=255) cGGAAGAGTTTCGCCACTGGCTATGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCg < 1:159328/148‑1 (MQ=255) gCCACTGGCTATGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAg > 1:206069/1‑148 (MQ=255) tGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGttt > 2:482479/1‑115 (MQ=255) tGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGttt < 1:482479/115‑1 (MQ=255) cgcgTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGtt < 1:105270/104‑1 (MQ=255) cgcgTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGtt > 2:105270/1‑104 (MQ=255) gcgTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGgag < 3:16773/148‑1 (MQ=255) cgTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGAt > 2:465276/1‑99 (MQ=255) cgTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGAt < 1:465276/99‑1 (MQ=255) cgTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAg < 1:548786/125‑1 (MQ=255) cgTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAg > 2:548786/1‑125 (MQ=255) cgTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCTTACGGTATGGGgaga < 4:122526/148‑1 (MQ=255) aTTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGt > 2:810619/1‑98 (MQ=255) aTTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGt < 1:810619/98‑1 (MQ=255) ggTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCttt < 2:206069/148‑1 (MQ=255) tCCGCTGTTTGAAGCGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCttttt > 4:1093454/1‑148 (MQ=255) ccGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCaa > 4:517013/1‑114 (MQ=255) ccGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCaa < 3:517013/114‑1 (MQ=255) gCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTg < 3:609776/147‑1 (MQ=255) gCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTg > 4:609776/1‑147 (MQ=255) tttGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATc > 1:234577/1‑97 (MQ=255) tttGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATc < 2:234577/97‑1 (MQ=255) aaGAGATGCACGCCGACCTGTTCCGCGCTGATTGCTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCt > 1:901430/1‑148 (MQ=255) aaGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGTAGATGt < 4:560217/82‑1 (MQ=255) aaGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGt > 3:560217/1‑82 (MQ=255) aaGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTa > 1:66259/1‑85 (MQ=255) aaGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTa < 2:66259/85‑1 (MQ=255) aaGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGAttt > 1:383807/1‑110 (MQ=255) aaGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGAttt < 2:383807/110‑1 (MQ=255) agaTGCACGCCGACCTGTTCCGCGCTGATTGCTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCTTcc < 2:901430/148‑1 (MQ=255) agaTGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCttttt > 3:79984/1‑134 (MQ=255) agaTGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCttttt < 4:79984/134‑1 (MQ=255) agaTGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCTTcc < 3:1093454/148‑1 (MQ=255) gaTGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATgg > 1:449828/1‑120 (MQ=255) gaTGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATgg < 2:449828/120‑1 (MQ=255) cGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCTTc > 4:1032853/1‑140 (MQ=255) cGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCTTc < 3:1032853/140‑1 (MQ=255) aCCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTGTCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCTTCCAtata < 1:288928/141‑1 (MQ=255) aCCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGAGTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCTTCCAtata > 2:288928/1‑141 (MQ=255) gTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTa > 1:998659/1‑72 (MQ=255) gTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTa < 2:998659/72‑1 (MQ=255) ttCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTg > 4:443506/1‑119 (MQ=255) ttCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTg < 3:443506/119‑1 (MQ=255) tCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATg < 1:156211/102‑1 (MQ=255) tCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGAGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATg > 2:156211/1‑102 (MQ=255) cgcTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATgggg < 1:191076/101‑1 (MQ=255) cgcTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATgggg > 2:191076/1‑101 (MQ=255) gATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTg > 4:648340/1‑110 (MQ=255) gATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTg < 3:648340/110‑1 (MQ=255) ttACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATg < 1:349099/100‑1 (MQ=255) ttACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATg > 2:349099/1‑100 (MQ=255) aaaaCCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCt < 3:323979/100‑1 (MQ=255) aaaaCCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCt > 4:323979/1‑100 (MQ=255) aCCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTaa < 3:753080/93‑1 (MQ=255) aCCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTaa > 4:753080/1‑93 (MQ=255) tGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCTTCCATATAAtt < 1:721574/98‑1 (MQ=255) tGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCTTCCATATAAtt > 2:721574/1‑98 (MQ=255) gAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCTTCCATATAATTTTTCTCCGCAATGTATCGAGGGTTATCCGTAAAGCCAAAGCTTTc < 4:597717/142‑1 (MQ=255) gAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCTTCCATATAATTTTTCTCCGCAATGTATCGAGGGTTATCCGTAAAGCCAAAGCTTTc > 3:597717/1‑142 (MQ=255) gAAGGGCATGTGGAAGACGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCTTCCATATAAtt > 3:112937/1‑97 (MQ=255) gAAGGGCATGTGGAAGACGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCTTCCATATAAtt > 3:779560/1‑97 (MQ=255) gAAGGGCATGTGGAAGACGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCTTCCATATAAtt < 4:779560/97‑1 (MQ=255) gAAGGGCATGTGGAAGACGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCTTCCATATAAtt > 1:487978/1‑97 (MQ=255) gAAGGGCATGTGGAAGACGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCTTCCATATAAtt < 4:112937/97‑1 (MQ=255) gAAGGGCATGTGGAAGACGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCTTCCATATAAtt < 2:487978/97‑1 (MQ=255) aaGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCTTCCATATAATTTTTCTCCGCAATGTATCGAg > 1:613931/1‑116 (MQ=255) aaGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCTTCCATATAATTTTTCTCCGCAATGTATCGAg < 2:613931/116‑1 (MQ=255) gtgGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCttt > 2:130022/1‑64 (MQ=255) gtgGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCttt < 1:130022/64‑1 (MQ=255) gATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCttt < 3:666958/58‑1 (MQ=255) gATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCttt > 4:666958/1‑58 (MQ=255) gATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCTTCCATATAATTTTTCTCCGCAATGTATCGAGGGTTATCCGTAAAGCCAAAGCtt < 1:857029/125‑1 (MQ=255) gATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCTTCCATATAATTTTTCTCCGCAATGTATCGAGGGTTATCCGTAAAGCCAAAGCtt > 2:857029/1‑125 (MQ=255) tGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCTTCCATATAATTTTTCTCCGCAATGTAt > 1:582575/1‑96 (MQ=255) tGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCTTCCATATAATTTTTCTCCGCAATGTAt < 2:582575/96‑1 (MQ=255) | GATGTTTTCCCGGAAGAGTTTCGCCACTGGCTATGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGAGTAAAGCTTCCATATAATTTTTCTCCGCAATGTATCGAGGGTTATCCGTAAAGCCAAAGCTTTC > NC_000913/4220129‑4220396 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |