Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,409,167 G→T 11.1% intergenic (+72/‑108) rnr → / → rlmB exoribonuclease R, RNase R/23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,409,1670GT11.1% 146.8 / 8.7 45intergenic (+72/‑108)rnr/rlmBexoribonuclease R, RNase R/23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (20/20);  new base T (3/2);  total (23/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

TAGCTGCAGCGACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATGATTTACGGCATCCACGCA  >  NC_000913/4409043‑4409304
                                                                                                                            |                                                                                                                                         
tAGCTGCAGCGACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGcc                                                                                                                                     <  1:741723/131‑1 (MQ=255)
tAGCTGCAGCGACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGcc                                                                                                                                     >  2:741723/1‑131 (MQ=255)
             cAAAGCGTAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATAAGGCGTTCACGccgcat                                                                                                                                 <  4:1001828/122‑5 (MQ=38)
             cAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATAAGGCGTTCACGccgcat                                                                                                                                 >  3:1001828/1‑118 (MQ=39)
               aagcgaagcgTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATAAGGCGTTCACGccgcatc                                                                                                                                >  2:229416/1‑116 (MQ=39)
                     agcgTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTg                                                                                               >  1:92207/1‑148 (MQ=255)
                      gcgTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGc                                                                                              <  2:92207/148‑1 (MQ=255)
                             atctagaaAAAAGTGGCAGAGAGAACAACACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGTCAAAATGCGCTTTGTACCTGGCGGATTCGACATGAAGGACTTATCCGGCCTCCGAAATCACGCAACTTCACTATAGTGCAGAGATc                                                                                       <  4:73229/144‑1 (MQ=255)
                                   aaaaaaGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATAAGGCGTTCACGccgcatc                                                                                                                                >  3:959645/1‑96 (MQ=38)
                                   aaaaaaGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATAAGGCGTTCACGccgcatc                                                                                                                                <  4:959645/101‑6 (MQ=37)
                                   aaaaaaGTGGCAGAGTGATCAATACCCACTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCAtagat                                                                                 >  3:73229/1‑144 (MQ=255)
                                    aaaaaGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGc                                                                                                                                      >  4:874525/1‑94 (MQ=255)
                                    aaaaaGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGc                                                                                                                                      <  3:874525/94‑1 (MQ=255)
                                      aaaGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTAt                                                                                                                                 >  2:20329/1‑97 (MQ=255)
                                      aaaGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTAt                                                                                                                                 <  1:20329/97‑1 (MQ=255)
                                      aaaGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACg                                                                                                               >  2:191652/1‑115 (MQ=255)
                                      aaaGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACg                                                                                                               <  1:191652/115‑1 (MQ=255)
                                                      cAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGcc                                                                                                                                     <  1:745683/77‑1 (MQ=255)
                                                      cAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGcc                                                                                                                                     >  2:745683/1‑77 (MQ=255)
                                                         tACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTa                                                                                  >  3:990589/1‑125 (MQ=255)
                                                         tACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTa                                                                                  <  4:990589/125‑1 (MQ=255)
                                                          aCCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAACATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                           <  3:776827/83‑1 (MQ=255)
                                                          aCCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                           >  4:776827/1‑83 (MQ=255)
                                                          aCCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCa                                                                                                       <  2:125341/103‑1 (MQ=255)
                                                          aCCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCa                                                                                                       >  1:125341/1‑103 (MQ=255)
                                                             ctctTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGaa                                                                                                                                         <  4:276519/66‑1 (MQ=255)
                                                             ctctTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGaa                                                                                                                                         >  3:276519/1‑66 (MQ=255)
                                                              tctTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGc                                                                                              >  3:577866/1‑108 (MQ=255)
                                                              tctTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGc                                                                                              <  4:577866/108‑1 (MQ=255)
                                                               ctTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAgaga                                                                                         >  3:1066627/1‑112 (MQ=255)
                                                               ctTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAgaga                                                                                         <  4:1066627/112‑1 (MQ=255)
                                                                  tAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACg                                                  >  2:138289/1‑148 (MQ=255)
                                                                       gaagaGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCa                                                                                      >  2:608720/1‑107 (MQ=255)
                                                                       gaagaGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCa                                                                                      <  1:608720/107‑1 (MQ=255)
                                                                        aatagGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAgaga                                                                                         <  1:349422/100‑1 (MQ=255)
                                                                        aagaGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAc                                                                                                                        >  3:636083/1‑72 (MQ=255)
                                                                        aagaGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAc                                                                                                                        <  4:636083/72‑1 (MQ=255)
                                                                        aagaGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAgaga                                                                                         >  2:349422/1‑103 (MQ=255)
                                                                                tAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGt                                                                    <  3:744867/116‑1 (MQ=255)
                                                                                tAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGt                                                                    >  4:744867/1‑116 (MQ=255)
                                                                                         gACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATg                             >  3:127247/1‑146 (MQ=255)
                                                                                         gACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATg                             <  4:127247/146‑1 (MQ=255)
                                                                                                         ttCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAatata                                                                                                  <  4:278579/61‑1 (MQ=255)
                                                                                                         ttCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAatata                                                                                                  >  3:278579/1‑61 (MQ=255)
                                                                                                                  gATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATGATTTACGGCATCCACGCa  <  1:138289/148‑1 (MQ=255)
                                                                                                                            |                                                                                                                                         
TAGCTGCAGCGACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATGATTTACGGCATCCACGCA  >  NC_000913/4409043‑4409304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: