Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,409,407	T→A	9.7%	S45T (TCT→ACT)	rlmB →	23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,409,407	0	T	A	9.7%	193.8 / 11.3	62	S45T (TCT→ACT)	rlmB	23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (28/28);  new base A (3/3);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

CGAAATGATTTACGGCATCCACGCAGTGCAGGCCCTGCTGGAGCGCGCCCCTGAACGTTTTCAGGAAGTCTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCT  >  NC_000913/4409280‑4409549                                                                                                                                |                                                                                                                                                cGAAATGATTTACGGCATCCACGCAGTGCAGGCCCTGCTGGAGCGCGCCCCTGAACGTTTTCAGGAAGTCTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGt                                                                                                                                      <  1:780905/138‑1 (MQ=255) cGAAATGATTTACGGCATCCACGCAGTGCAGGCCCTGCTGGAGCGCGCCCCTGAACGTTTTCAGGAAGTCTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGt                                                                                                                                      >  2:780905/1‑138 (MQ=255)   aaaTGATTTACGGCATCCACGCAGTGCAGGCCCTGCTGGAGCGCGCCCCTGAACGTTTTCAGGAAGTCTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTg                                                                                                                                     <  3:259486/137‑1 (MQ=255)   aaaTGATTTACGGCATCCACGCAGTGCAGGCCCTGCTGGAGCGCGCCCCTGAACGGTTTCAGGAAGTCTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTg                                                                                                                                     >  4:259486/1‑137 (MQ=255)                                gCCCTGCTGGAGCGCGCCCCTGAACGTTTTCAGGAAGTCTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTg                                                                                                                                     <  2:544649/108‑1 (MQ=255)                                gCCCTGCTGGAGCGCGCCCCTGAACGTTTTCAGGAAGTCTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTg                                                                                                                                     >  1:544649/1‑108 (MQ=255)                                   ctgctgGAGCGCGCCCCTGAACGTTTTCAGGAAGTCTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGt                                                                                                                                      <  1:346209/104‑1 (MQ=255)                                   ctgctgGAGCGCGCCCCTGAACGTTTTCAGGAAGTCTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGt                                                                                                                                      >  2:346209/1‑104 (MQ=255)                                                                ggAAGTCTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGtt                                                                                                                                  <  2:879673/79‑1 (MQ=255)                                                                ggAAGTCTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGtt                                                                                                                                  >  1:879673/1‑79 (MQ=255)                                                                  aaGTCTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGtctc                                                                                                                                              >  4:974575/1‑66 (MQ=255)                                                                  aaGTCTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGtctc                                                                                                                                              <  3:974575/66‑1 (MQ=255)                                                                  aaGTCTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTg                                                                                                                                     >  3:901813/1‑74 (MQ=255)                                                                  aaGTCTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTg                                                                                                                                     <  4:901813/74‑1 (MQ=255)                                                                  aaGTCTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTcgacg                                                                                                   <  3:228811/108‑1 (MQ=255)                                                                  aaGTCTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTcgacg                                                                                                   >  4:228811/1‑108 (MQ=255)                                                                      cTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGc                                                                                                                <  3:105972/91‑1 (MQ=255)                                                                      cTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGc                                                                                                                >  4:105972/1‑91 (MQ=255)                                                                        ttATTTTAAAAGGCCGTGAAGATAAACGTCTGTTATCGCTGATTCACGCCCTTGAGtctctg                                                                                                                                            >  1:279423/1‑60 (MQ=255)                                                                        ttATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑Ag                                                                                                                                           <  2:279423/62‑1 (MQ=255)                                                                        ttATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTcg                                                                                                      >  2:17521/1‑99 (MQ=255)                                                                        ttATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTcg                                                                                                      <  1:17521/99‑1 (MQ=255)                                                                            tttAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCg                                                                                          <  1:1103420/107‑1 (MQ=255)                                                                            tttAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCg                                                                                          >  2:1103420/1‑107 (MQ=255)                                                                            tttAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTg                                                                               >  2:546884/1‑118 (MQ=255)                                                                            tttAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTg                                                                               <  1:546884/118‑1 (MQ=255)                                                                                   ggCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGt                                                                                                                           >  3:945206/1‑67 (MQ=255)                                                                                   ggCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGt                                                                                                                           <  4:945206/67‑1 (MQ=255)                                                                                        tGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTcg                                                                                                      <  1:148486/83‑1 (MQ=255)                                                                                        tGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTcg                                                                                                      >  2:148486/1‑83 (MQ=255)                                                                                        tGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAg                                                                         <  2:875226/112‑1 (MQ=255)                                                                                        tGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAg                                                                         >  1:875226/1‑112 (MQ=255)                                                                                         gAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCg                                                               >  1:1072757/1‑121 (MQ=255)                                                                                         gAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCg                                                               <  2:1072757/121‑1 (MQ=255)                                                                                            gATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAgg                                 >  1:552601/1‑148 (MQ=255)                                                                                              tAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAg                                                                         >  1:858576/1‑106 (MQ=255)                                                                                              tAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAg                                                                         <  2:858576/106‑1 (MQ=255)                                                                                               aaaCGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATc                                    <  3:34987/142‑1 (MQ=255)                                                                                               aaaCGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATc                                    >  4:34987/1‑142 (MQ=255)                                                                                                aaCGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCa                                                 >  3:103028/1‑128 (MQ=255)                                                                                                aaCGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCa                                                 <  4:103028/128‑1 (MQ=255)                                                                                                    tCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGAt                         <  2:552601/148‑1 (MQ=255)                                                                                                        ttACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATAtc                                                                                                         <  4:898190/64‑1 (MQ=255)                                                                                                        ttACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATAtc                                                                                                         >  3:898190/1‑64 (MQ=255)                                                                                                         tACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCa                                                 >  2:709548/1‑119 (MQ=255)                                                                                                         tACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCa                                                 <  1:709548/119‑1 (MQ=255)                                                                                                               tGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGa                 <  3:683901/145‑1 (MQ=255)                                                                                                               tGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGa                 >  4:683901/1‑145 (MQ=255)                                                                                                                     aCGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGcc                                                  >  4:319436/1‑106 (MQ=255)                                                                                                                     aCGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGcc                                                  <  3:319436/106‑1 (MQ=255)                                                                                                                     aCGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCa                                                 >  4:128020/1‑107 (MQ=255)                                                                                                                     aCGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCa                                                 <  3:128020/107‑1 (MQ=255)                                                                                                                     aCGCCC‑TGAGACTCGAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCAt                                                                            >  4:1094888/1‑80 (MQ=255)                                                                                                                     aCGCCC‑TGAGACTCAAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCg                                                                                 <  3:943466/75‑1 (MQ=38)                                                                                                                     aCGCCC‑TGAGACTCAAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCg                                                                                 >  4:943466/1‑75 (MQ=37)                                                                                                                     aCGCCC‑TGAGACTCAAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCAt                                                                            <  3:1094888/80‑1 (MQ=38)                                                                                                                     aCGCCC‑TGAGACTCAAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGa            <  3:873246/144‑1 (MQ=255)                                                                                                                     aCGCCC‑TGAGACTCAAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGa            >  4:873246/1‑144 (MQ=255)                                                                                                                      cGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAg                                                                         >  4:667616/1‑82 (MQ=255)                                                                                                                      cGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAg                                                                         <  3:667616/82‑1 (MQ=255)                                                                                                                      cGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGGAGCCAGGACGTCAGTATc                                    <  4:651420/119‑1 (MQ=255)                                                                                                                      cGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATc                                    >  3:651420/1‑119 (MQ=255)                                                                                                                        cccTTGAGTCTC‑AGGTCGGGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTc                                          >  4:251446/1‑111 (MQ=255)                                                                                                                        cccTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTc                                          <  3:251446/111‑1 (MQ=255)                                                                                                                           ttGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGAtcgcttcgct  >  1:435396/1‑148 (MQ=255)                                                                                                                                |                                                                                                                                                CGAAATGATTTACGGCATCCACGCAGTGCAGGCCCTGCTGGAGCGCGCCCCTGAACGTTTTCAGGAAGTCTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTC‑AGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCT  >  NC_000913/4409280‑4409549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑