Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,433,318 A→T 6.3% L236Q (CTG→CAG)  qorB ← NAD(P)H:quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,433,3180AT 6.3% 405.1 / 12.9 111L236Q (CTG→CAG) qorBNAD(P)H:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (53/51);  new base T (2/5);  total (55/56)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 111 total aligned reads displayed.

CATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTGGTCAGCTCTGCCGCTAACTGTGTCAACGTCCAGGCACTATCGCCCGCCAGTTCGTAA  >  NC_000913/4433175‑4433452
                                                                                                                                               |                                                                                                                                      
cATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTcc                                                                                                                                    <  4:452732/148‑1 (MQ=255)
  ttAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGa                                                                                                                                  >  4:1127860/1‑148 (MQ=255)
      aaaGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCt                                                                                                                              >  3:42596/1‑148 (MQ=255)
      aaaGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGACTGCCCGACgga                                                                                                                              <  1:953222/148‑3 (MQ=255)
             gCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGt                                                                                                                       >  1:383617/1‑148 (MQ=255)
             gCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGACTGCCCGACgga                                                                                                                              <  2:480379/141‑3 (MQ=255)
                      ttCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCgcggcg                                                                                                              >  1:598185/1‑148 (MQ=255)
                                 ttGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTc                                                                                                   >  1:971560/1‑148 (MQ=255)
                                   gTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCgg                                                                                                       >  2:799718/1‑142 (MQ=255)
                                   gTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCgg                                                                                                       <  1:799718/142‑1 (MQ=255)
                                       ttGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCtttt                                                                                                                           >  1:1025275/1‑118 (MQ=255)
                                       ttGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCtttt                                                                                                                           <  2:1025275/118‑1 (MQ=255)
                                       ttGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAg                                                                                             >  1:743595/1‑148 (MQ=255)
                                          ggTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGAtt                                                                                          >  2:312189/1‑148 (MQ=255)
                                             ggCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTg                                                                                       <  2:743595/148‑1 (MQ=255)
                                             ggCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTg                                                                                       <  2:971560/148‑1 (MQ=255)
                                               ccAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGAt                                                                                     <  2:598185/148‑1 (MQ=255)
                                                 aatcaatTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTGGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTg                                                                                       >  4:979375/1‑144 (MQ=255)
                                                    caatTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGc                                                                                                                               >  3:793842/1‑101 (MQ=255)
                                                      atTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCgg                                                                                                       <  4:570090/123‑1 (MQ=255)
                                                      atTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCgg                                                                                                       >  3:570090/1‑123 (MQ=255)
                                                      atTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAAc                                                                              >  4:487353/1‑148 (MQ=255)
                                                       tttGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTc                                                                                                                          <  2:95391/103‑1 (MQ=255)
                                                       tttGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTc                                                                                                                          >  1:95391/1‑103 (MQ=255)
                                                       tttGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAg                                                                                                                        <  4:1110325/105‑1 (MQ=255)
                                                       tttGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAg                                                                                                                        >  3:1110325/1‑105 (MQ=255)
                                                       tttGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGaa                                                                                                            <  2:121226/117‑1 (MQ=255)
                                                       tttGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGaa                                                                                                            >  1:121226/1‑117 (MQ=255)
                                                         tGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTg                                                                           <  1:312189/148‑1 (MQ=255)
                                                               gtgtTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCgg                                                                                                       <  2:519110/114‑1 (MQ=255)
                                                                 gtTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTc                                                                                               >  3:89624/1‑120 (MQ=255)
                                                                 gtTTTGCTGTAATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTc                                                                                               <  4:89624/120‑1 (MQ=255)
                                                                   tttGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGc                                                                      >  1:913510/1‑143 (MQ=255)
                                                                   tttGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGc                                                                      <  2:913510/143‑1 (MQ=255)
                                                                    ttGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAAt                                                                                                          >  1:822149/1‑106 (MQ=255)
                                                                    ttGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAAt                                                                                                          <  2:822149/106‑1 (MQ=255)
                                                                      gCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGaa                                                                                                            <  2:429033/102‑1 (MQ=255)
                                                                      gCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGaa                                                                                                            >  1:429033/1‑102 (MQ=255)
                                                                       cTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGtt                                                             <  2:383617/148‑1 (MQ=255)
                                                                         gTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTa                                                                                >  4:456718/1‑127 (MQ=255)
                                                                         gTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTa                                                                                <  3:456718/127‑1 (MQ=255)
                                                                             tcaAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGAttt                                                                                         >  1:1055807/1‑114 (MQ=255)
                                                                             tcaAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGAttt                                                                                         <  2:1055807/114‑1 (MQ=255)
                                                                             tcaAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTg                                                                                       >  4:664069/1‑116 (MQ=255)
                                                                             tcaAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTaa                                                                               >  2:410159/1‑124 (MQ=255)
                                                                             tcaAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTaa                                                                               <  1:410159/124‑1 (MQ=255)
                                                                              caAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTg                                                                                       <  3:831967/115‑1 (MQ=255)
                                                                              caAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTg                                                                                       >  4:831967/1‑115 (MQ=255)
                                                                              caAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGACTGCCCGACg                                                                                                                                >  3:1122967/1‑74 (MQ=255)
                                                                              caAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGACTGCCCGACg                                                                                                                                <  4:1122967/74‑1 (MQ=255)
                                                                              caAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGACTGCCCGACg                                                                                                                                <  4:226982/74‑1 (MQ=255)
                                                                              caAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGACTGCCCGACg                                                                                                                                <  2:164628/74‑1 (MQ=255)
                                                                                aaCAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTGGTCAGct                                                    >  2:764756/1‑148 (MQ=255)
                                                                                  cAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGttt                                                            <  3:635578/138‑1 (MQ=255)
                                                                                  cAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGttt                                                            >  4:635578/1‑138 (MQ=255)
                                                                                     aCCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGa                                                                                      >  2:827999/1‑109 (MQ=255)
                                                                                     aCCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGa                                                                                      <  1:827999/109‑1 (MQ=255)
                                                                                       cGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTg                                                                                       <  3:979597/106‑1 (MQ=255)
                                                                                         ccTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTg                                                               >  3:848753/1‑128 (MQ=255)
                                                                                         ccTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTg                                                               <  4:848753/128‑1 (MQ=255)
                                                                                           tttCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTGGTCAGCTCTGCCGCTAAc                                         <  1:15775/148‑1 (MQ=255)
                                                                                                aTGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGt                                                                          >  3:1100298/1‑110 (MQ=255)
                                                                                                aTGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGt                                                                          <  4:1100298/110‑1 (MQ=255)
                                                                                                aTGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTGGTCAGCTCTGCCGCTAACTgtgt                                    <  1:764756/148‑1 (MQ=255)
                                                                                                  gcgcCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCgg                                                                                                       >  4:799689/1‑79 (MQ=255)
                                                                                                  gcgcCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCgg                                                                                                       <  3:799689/79‑1 (MQ=255)
                                                                                                     ccAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGct                                                                  >  1:520914/1‑113 (MQ=255)
                                                                                                       aaCGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTGGTCAGCTCTGCCGCTAACTGTGTCAACGTc                             >  2:723446/1‑148 (MQ=255)
                                                                                                         cGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTGGTCAGCTCTGCCGCTAACTGTGTCAACGTCCa                           <  3:487353/148‑1 (MQ=255)
                                                                                                           tCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTGGTCAGct                                                    >  2:6455/1‑121 (MQ=255)
                                                                                                           tCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTGGTCAGct                                                    <  1:6455/121‑1 (MQ=255)
                                                                                                            cAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGAtt                                                                                          <  2:343304/82‑1 (MQ=255)
                                                                                                            cAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGAtt                                                                                          >  1:343304/1‑82 (MQ=255)
                                                                                                            cAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGt                                                              <  4:498970/110‑1 (MQ=255)
                                                                                                            cAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGt                                                              >  3:498970/1‑110 (MQ=255)
                                                                                                                aTCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGAt                                                                                     <  3:876926/83‑1 (MQ=255)
                                                                                                                aTCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGAt                                                                                     >  4:876926/1‑83 (MQ=255)
                                                                                                                aTCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTgg                                                          <  3:482493/110‑1 (MQ=255)
                                                                                                                aTCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTgg                                                          >  4:482493/1‑110 (MQ=255)
                                                                                                                aTCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTGGTCAGCTCTGCCGCTAACTGTGTCAACGTCCAGGc                        >  3:1023155/1‑144 (MQ=255)
                                                                                                                aTCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTGGTCAGCTCTGCCGCTAACTGTGTCAACGTCCAGGc                        <  4:1023155/144‑1 (MQ=255)
                                                                                                                     ccAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGAtttt                                                                                        >  2:257877/1‑75 (MQ=255)
                                                                                                                     ccAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGAtttt                                                                                        <  1:257877/75‑1 (MQ=255)
                                                                                                                         cATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTGGTCAGCTCTGCCGCTAACTGTGTCAACGTCCa                           >  1:992803/1‑132 (MQ=255)
                                                                                                                         cATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTGGTCAGCTCTGCCGCTAACTGTGTCAACGTCCa                           <  2:992803/132‑1 (MQ=255)
                                                                                                                         cATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTGGTCAGCTCTGCCGCTAACTGTGTCAACGTCCAg                          <  1:995150/133‑1 (MQ=255)
                                                                                                                         cATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTGGTCAGCTCTGCCGCTAACTGTGTCAACGTCCAg                          >  2:995150/1‑133 (MQ=255)
                                                                                                                           tatCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTGGTCAGCTCTg                                                 <  3:939292/108‑1 (MQ=255)
                                                                                                                           tatCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTGGTCAGCTCTg                                                 >  4:939292/1‑108 (MQ=255)
                                                                                                                            atCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTGGTCAGCTCTGCCGCTAACTGTGTCAACGTCCa                           >  3:911388/1‑129 (MQ=255)
                                                                                                                            atCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTGGTCAGCTCTGCCGCTAACTGTGTCAACGTCCa                           <  4:911388/129‑1 (MQ=255)
                                                                                                                             tCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTGGTCAGCTCTGCCGCTAACTGTGTCAACGTCCAGGCACTATCGCCCGCCAGtt       >  4:697992/1‑148 (MQ=255)
                                                                                                                             tCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTGGTCAGCTCTACCGCTAACTGTGTCAACGTCCAGGCACTATCGCCCGCCAGtt       <  3:1127860/148‑1 (MQ=255)
                                                                                                                              ccGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGa                                                                                      >  3:323131/1‑68 (MQ=255)
                                                                                                                              ccGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGa                                                                                      <  4:323131/68‑1 (MQ=255)
                                                                                                                                  cAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTGGTCAGCTCTGCCGCTAACTGTGTCAACGTCCAGGCACTATCGCCCGCCAGTTCGTaa  >  1:937984/1‑148 (MQ=255)
                                                                                                                                   aGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTGGTCAGCTCTGCCGCTAACTGTGTCAACGTCCAGGCACTATcgcccg             >  3:867797/1‑136 (MQ=255)
                                                                                                                                   aGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTGGTCAGCTCTGCCGCTAACTGTGTCAACGTCCAGGCACTATcgcccg             <  4:867797/136‑1 (MQ=255)
                                                                                                                                         tCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTGGTCAGCTCTGCCGCTAACTgtgt                                    <  3:346575/107‑1 (MQ=255)
                                                                                                                                         tCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTGGTCAGCTCTGCCGCTAACTgtgt                                    >  4:346575/1‑107 (MQ=255)
                                                                                                                                               |                                                                                                                                      
CATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGATTTTGATAGGTAACCTGTTTGCCGCTCTGTTTGGTCAGCTCTGCCGCTAACTGTGTCAACGTCCAGGCACTATCGCCCGCCAGTTCGTAA  >  NC_000913/4433175‑4433452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: