| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 443,022 | G→A | 36.4% | intergenic (+25/+29) | thiI → / ← yajL | tRNA s(4)U8 sulfurtransferase/oxidative‑stress‑resistance chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 443,022 | 0 | G | A | 36.4% | 15.5 / 19.8 | 22 | intergenic (+25/+29) | thiI/yajL | tRNA s(4)U8 sulfurtransferase/oxidative‑stress‑resistance chaperone |
| Reads supporting (aligned to +/- strand): ref base G (7/7); new base A (4/4); total (11/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
ACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTC > NC_000913/442888‑443144 | aCCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAg > 2:799349/1‑139 (MQ=255)aCCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAg < 1:799349/139‑1 (MQ=255) aCCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAg < 1:1094940/128‑1 (MQ=255) aCCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAg > 2:1094940/1‑128 (MQ=255) tttAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaataa < 4:477063/96‑1 (MQ=255) tttAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaataa > 3:477063/1‑96 (MQ=255) cGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGt > 1:22447/1‑98 (MQ=255) cGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGt < 2:22447/98‑1 (MQ=255) tttttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATaa > 1:636172/1‑65 (MQ=255) tttttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATaa < 2:636172/65‑1 (MQ=255) ttttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGa > 1:172273/1‑90 (MQ=255) ttttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGa < 2:172273/90‑1 (MQ=255) cGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGt < 2:312245/77‑1 (MQ=255) cGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGt > 1:312245/1‑77 (MQ=255) cGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGa > 2:386752/1‑82 (MQ=255) cGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGa < 1:386752/82‑1 (MQ=255) cGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCccaacca < 3:193214/114‑1 (MQ=255) cGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCccaacca > 4:193214/1‑114 (MQ=255) cGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTc > 2:718197/1‑131 (MQ=255) cGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTc < 1:718197/131‑1 (MQ=255) gCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATg > 3:1061560/1‑93 (MQ=255) gCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATg < 4:1061560/93‑1 (MQ=255) | ACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTC > NC_000913/442888‑443144 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |