Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,454,801 A→C 5.6% L270R (CTG→CGG) ‡ fbp ← fructose‑1,6‑bisphosphatase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,454,8010AC 5.6% 320.3 / 9.3 89L270R (CTG→CGG) ‡fbpfructose‑1,6‑bisphosphatase I
Reads supporting (aligned to +/- strand):  ref base A (43/41);  new base C (3/2);  total (46/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

ACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTATCTTCTTCCTGGCAGAATT  >  NC_000913/4454654‑4454945
                                                                                                                                                   |                                                                                                                                                
aCCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCa                                                                                                                                                  <  4:959040/148‑1 (MQ=255)
      tGGTCGTTGCCGACAAAGAATGATCGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACTGCGTTTGc                                                                                                                                            <  1:138542/148‑1 (MQ=255)
                 gACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTc                                                                                                                                        >  4:667905/1‑141 (MQ=255)
                 gACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTc                                                                                                                                        <  3:667905/141‑1 (MQ=255)
                  aCAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAACCGCAGTTTGCCGTCCGggcggc                                                                                                                                >  4:404152/1‑148 (MQ=255)
                  aCAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGtggc                                                                                                                                >  2:1021006/1‑148 (MQ=255)
                         aTGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGt                                                                                                                         >  4:768056/1‑148 (MQ=255)
                           gAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAGTACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCGTGTGCCGCCAGGAATGCCATCGGGTTGCACTCAGACGGCAAACGCAGTTGGCCGTCCGGGTGGCTGGCGGTGc                                                                                                                       <  3:404152/148‑1 (MQ=255)
                             aCGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCtt                                                                                                                     <  3:477338/148‑1 (MQ=255)
                               ggCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTgg                                                                                                                   <  1:1021006/148‑1 (MQ=255)
                                           gggTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACTGCGTTTGct                                                                                                                                           >  3:927036/1‑111 (MQ=255)
                                                    ggATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACTGCGTTTGctg                                                                                                                                          >  1:94010/1‑102 (MQ=255)
                                                      atgatATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACTGCGTTTGctgta                                                                                                                                        >  2:1115298/1‑100 (MQ=255)
                                                      atgatATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACTGCGTTTGCTGta                                                                                                                                        <  1:1115298/104‑2 (MQ=255)
                                                             ccAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCa                                                                                                                                                  >  4:193583/1‑87 (MQ=255)
                                                             ccAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCa                                                                                                                                                  <  3:193583/87‑1 (MQ=255)
                                                             ccAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGtggc                                                                                                                                >  2:84249/1‑105 (MQ=255)
                                                             ccAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGtggc                                                                                                                                <  1:84249/105‑1 (MQ=255)
                                                             ccAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAgaga                                                                                                            <  2:904136/125‑1 (MQ=255)
                                                             ccAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAgaga                                                                                                            >  1:904136/1‑125 (MQ=255)
                                                               aGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTg                                                                                                                    >  3:917728/1‑115 (MQ=255)
                                                               aGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTg                                                                                                                    <  4:917728/115‑1 (MQ=255)
                                                               aGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCt                                                                                                >  1:881319/1‑135 (MQ=255)
                                                               aGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCt                                                                                                <  2:881319/135‑1 (MQ=255)
                                                                 aaTACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAg                                                                                                                                                 <  1:930901/84‑1 (MQ=255)
                                                                 aaTACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAg                                                                                                                                                 >  2:930901/1‑84 (MQ=255)
                                                                  aTACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCggg                                                                                                                                    >  3:918859/1‑96 (MQ=255)
                                                                  aTACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCggg                                                                                                                                    <  4:918859/96‑1 (MQ=255)
                                                                       ctctTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCtt                                                                                                                     >  3:962486/1‑106 (MQ=255)
                                                                       ctctTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCtt                                                                                                                     <  4:962486/106‑1 (MQ=255)
                                                                                  cgctcgctTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATaa                                                                                                         <  2:1062184/107‑1 (MQ=255)
                                                                                  cgctcgctTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATaa                                                                                                         >  1:1062184/1‑107 (MQ=255)
                                                                                    ctcgctTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTg                                                                                                                        >  4:723106/1‑90 (MQ=255)
                                                                                    ctcgctTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTg                                                                                                                        <  3:723106/90‑1 (MQ=255)
                                                                                        ctTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTggg                                                                                                                  >  2:837679/1‑92 (MQ=255)
                                                                                        ctTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTggg                                                                                                                  <  1:837679/92‑1 (MQ=255)
                                                                                          ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGa                                                        <  3:768056/148‑1 (MQ=255)
                                                                                                cccgcTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAAc                                                  >  3:684866/1‑148 (MQ=255)
                                                                                                 ccgcTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGaa                                                            >  1:349517/1‑137 (MQ=255)
                                                                                                 ccgcTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGaa                                                            <  2:349517/137‑1 (MQ=255)
                                                                                                 ccgcTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACg                                                 >  2:766936/1‑148 (MQ=255)
                                                                                                   gcTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTc                                                                                             <  4:593681/102‑1 (MQ=255)
                                                                                                   gcTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTc                                                                                             >  3:593681/1‑102 (MQ=255)
                                                                                                   gcTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTg                                               <  4:684866/148‑1 (MQ=255)
                                                                                                    cTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTg                                                                               <  4:259169/115‑1 (MQ=255)
                                                                                                    cTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTg                                                                               >  3:259169/1‑115 (MQ=255)
                                                                                                    cTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGa                                              <  1:766936/148‑1 (MQ=255)
                                                                                                         tCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTg                                                                                                                    >  2:345838/1‑73 (MQ=255)
                                                                                                         tCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTg                                                                                                                    <  1:345838/73‑1 (MQ=255)
                                                                                                         tCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTag                                                                                                               >  1:1127433/1‑78 (MQ=255)
                                                                                                         tCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTag                                                                                                               <  2:1127433/78‑1 (MQ=255)
                                                                                                              cAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTAc                                                                                   >  4:1036727/1‑101 (MQ=255)
                                                                                                              cAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTAc                                                                                   <  3:1036727/101‑1 (MQ=255)
                                                                                                              cAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGa                                                        >  4:998650/1‑128 (MQ=255)
                                                                                                              cAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGACTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGa                                                        <  3:998650/128‑1 (MQ=255)
                                                                                                                gggaTGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTaga                                                                                                              >  2:320698/4‑72 (MQ=255)
                                                                                                                ggAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTaga                                                                                                              <  1:320698/72‑1 (MQ=255)
                                                                                                                        aTCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGtggctggc                                                                                                                            <  3:239010/50‑1 (MQ=255)
                                                                                                                        aTCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGtggctggc                                                                                                                            >  4:239010/1‑50 (MQ=255)
                                                                                                                        aTCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCg                                                         >  1:1047206/1‑117 (MQ=255)
                                                                                                                        aTCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCg                                                         <  2:1047206/117‑1 (MQ=255)
                                                                                                                         tCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGa                         >  3:790457/1‑148 (MQ=255)
                                                                                                                          cGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAgg                                                                                       <  1:802607/85‑1 (MQ=255)
                                                                                                                          cGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAgg                                                                                       <  4:580225/85‑1 (MQ=255)
                                                                                                                          cGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAgg                                                                                       >  3:580225/1‑85 (MQ=255)
                                                                                                                          cGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAgg                                                                                       >  2:802607/1‑85 (MQ=255)
                                                                                                                          cGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATc                                                                         >  3:406278/1‑99 (MQ=255)
                                                                                                                          cGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATc                                                                         <  4:406278/99‑1 (MQ=255)
                                                                                                                                 cACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTg                                               <  2:27344/118‑1 (MQ=255)
                                                                                                                                 cACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTg                                               >  1:27344/1‑118 (MQ=255)
                                                                                                                                   cTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAAt                                                                          >  3:93055/1‑89 (MQ=255)
                                                                                                                                   cTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAAt                                                                          <  4:93055/89‑1 (MQ=255)
                                                                                                                                   cTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCgg                                 <  2:1009574/130‑1 (MQ=255)
                                                                                                                                   cTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCgg                                 >  1:1009574/1‑130 (MQ=255)
                                                                                                                                    tCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTAtcttct               >  2:342171/1‑147 (MQ=255)
                                                                                                                                    tCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTAtcttct               <  1:342171/147‑1 (MQ=255)
                                                                                                                                     cATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATaa                                                   >  3:759558/1‑110 (MQ=255)
                                                                                                                                     cATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATaa                                                   <  4:759558/110‑1 (MQ=255)
                                                                                                                                       tACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGAtata                                                    >  2:457979/1‑107 (MQ=255)
                                                                                                                                       tACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGAtata                                                    <  1:457979/107‑1 (MQ=255)
                                                                                                                                        aCAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTATCTTCTTCCTg          <  4:790457/148‑1 (MQ=255)
                                                                                                                                            cAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTa                                                                                    >  2:942847/1‑70 (MQ=255)
                                                                                                                                            cAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTa                                                                                    <  1:942847/70‑1 (MQ=255)
                                                                                                                                            cAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGa                                              <  2:60680/108‑1 (MQ=255)
                                                                                                                                            cAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGa                                              >  1:60680/1‑108 (MQ=255)
                                                                                                                                             aaaCGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTATCTTCTTCCTGGCAGa     >  4:452872/1‑148 (MQ=255)
                                                                                                                                             aaaCGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTATCTTCTTCCTGGCAGa     >  4:91988/1‑148 (MQ=255)
                                                                                                                                               aCGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGa                         >  2:569224/1‑126 (MQ=255)
                                                                                                                                               aCGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGa                         <  1:569224/126‑1 (MQ=255)
                                                                                                                                               aCGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTa                     >  1:343607/1‑130 (MQ=255)
                                                                                                                                               aCGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTa                     <  2:343607/130‑1 (MQ=255)
                                                                                                                                                cGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTATCTTCTTCCTGGCAGAAtt  >  1:392564/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
ACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTATCTTCTTCCTGGCAGAATT  >  NC_000913/4454654‑4454945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: