Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,457,257 T→C 9.1% intergenic (+99/+57) mpl → / ← yjgA UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase/ribosome‑associated UPF0307 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,457,2570TC 9.1% 211.0 / 8.6 66intergenic (+99/+57)mpl/yjgAUDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase/ribosome‑associated UPF0307 family protein
Reads supporting (aligned to +/- strand):  ref base T (31/29);  new base C (3/3);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

CAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCG  >  NC_000913/4457111‑4457402
                                                                                                                                                  |                                                                                                                                                 
cAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTa                                                                                                                                                  >  4:16065/1‑148 (MQ=255)
   aaaCTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCa                                                                                                                                               <  1:1094039/148‑1 (MQ=255)
             aTGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATc                                                                                                                                                <  1:186059/137‑1 (MQ=255)
             aTGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATc                                                                                                                                                >  2:186059/1‑137 (MQ=255)
                                      cgcgCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGt                                                                                                                             >  4:297624/1‑131 (MQ=255)
                                      cgcgCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGt                                                                                                                             <  3:297624/131‑1 (MQ=255)
                                        cgcAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTc                                                                                                           <  4:376645/147‑1 (MQ=255)
                                        cgcAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTc                                                                                                           >  3:376645/1‑147 (MQ=255)
                                                        tAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTc                                                                                                           >  2:859327/1‑131 (MQ=255)
                                                        tAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTc                                                                                                           <  1:859327/131‑1 (MQ=255)
                                                        tAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAAt                                                                                          >  4:1045875/1‑148 (MQ=255)
                                                            cAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTAcc                                                                                      >  2:767648/1‑148 (MQ=255)
                                                            cAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTAcc                                                                                      >  2:1118000/1‑148 (MQ=255)
                                                            cAGATAGAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATATTCACATCAACAACGAAAATTAcc                                                                                      >  2:488983/1‑148 (MQ=255)
                                                               atatAGTATGCGTAGGCCGGATAAGGGGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCtt                                                                                   <  1:488983/148‑1 (MQ=255)
                                                                       tGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTAt                                                                                                                           <  3:71444/100‑1 (MQ=255)
                                                                       tGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTAt                                                                                                                           >  4:71444/1‑100 (MQ=255)
                                                                        gCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCg                                                                          <  3:1045875/148‑1 (MQ=255)
                                                                           tAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTa                                                                       <  3:16065/148‑1 (MQ=255)
                                                                                  gATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTAt                                                                                                                                                 <  3:35178/67‑1 (MQ=255)
                                                                                  gATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTAt                                                                                                                                                 >  4:35178/1‑67 (MQ=255)
                                                                                   aTAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCgcgc                                                               <  1:767648/148‑1 (MQ=255)
                                                                                   aTAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCgcgc                                                               <  1:1118000/148‑1 (MQ=255)
                                                                                    tAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAAc                                                                                                                  >  4:159723/1‑96 (MQ=255)
                                                                                    tAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAAc                                                                                                                  <  3:159723/96‑1 (MQ=255)
                                                                                     aaGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTAc                                                                                       >  2:1017424/1‑122 (MQ=255)
                                                                                     aaGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTAc                                                                                       <  1:1017424/122‑1 (MQ=255)
                                                                                           ttCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAAt                                                                                          >  3:996358/1‑113 (MQ=255)
                                                                                           ttCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAAt                                                                                          <  4:996358/113‑1 (MQ=255)
                                                                                                 ccgcATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGc                                                                         <  2:576665/124‑1 (MQ=255)
                                                                                                 ccgcATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGc                                                                         >  1:576665/1‑124 (MQ=255)
                                                                                                  cgcATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTaa                                                                      >  4:417821/1‑126 (MQ=255)
                                                                                                  cgcATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTaa                                                                      <  3:417821/126‑1 (MQ=255)
                                                                                                  cgcATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATc                                                >  1:1117104/1‑148 (MQ=255)
                                                                                                     aTCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTtctc                                                                            <  1:243117/117‑1 (MQ=255)
                                                                                                     aTCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTtctc                                                                            >  2:243117/1‑117 (MQ=255)
                                                                                                       ccGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCg                                           <  2:1117104/148‑1 (MQ=255)
                                                                                                          gCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACATGTGc                                                                                                                                  <  1:321407/58‑1 (MQ=255)
                                                                                                          gCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGc                                                                                                                                  >  2:321407/1‑58 (MQ=255)
                                                                                                          gCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACTACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCGGATACTgg                                                      <  2:315804/134‑1 (MQ=255)
                                                                                                          gCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTgg                                                      >  1:315804/1‑134 (MQ=255)
                                                                                                             tttGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCtt                                                                                   >  3:552417/1‑102 (MQ=255)
                                                                                                             tttGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCtt                                                                                   <  4:552417/102‑1 (MQ=255)
                                                                                                                   cATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACt                                                                    >  2:580327/1‑111 (MQ=255)
                                                                                                                   cATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACt                                                                    <  1:580327/111‑1 (MQ=255)
                                                                                                                    aTAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCg                                                                                                                              >  2:389840/1‑52 (MQ=255)
                                                                                                                    aTAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCg                                                                                                                              <  1:389840/52‑1 (MQ=255)
                                                                                                                         gCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACt                                                        <  4:50737/117‑1 (MQ=255)
                                                                                                                         gCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACt                                                        >  3:50737/1‑117 (MQ=255)
                                                                                                                          ccTGATAAGACGCATCAAGCGTCGCATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTa                                                                                        <  2:23814/84‑1 (MQ=21)
                                                                                                                          ccTGATAAGACGCATCAAGCGTCGCATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTa                                                                                        >  1:23814/1‑84 (MQ=21)
                                                                                                                               taagACGCATCAAGCGTCGCATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTgg                                                      <  1:84000/110‑1 (MQ=255)
                                                                                                                               taagACGCATCAAGCGTCGCATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTgg                                                      >  2:84000/4‑113 (MQ=255)
                                                                                                                               taagACGCATCAAGCGTCGCATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTCTTccc                   >  2:755323/4‑148 (MQ=255)
                                                                                                                                   aCGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACt                                                                                                            <  4:568418/55‑1 (MQ=255)
                                                                                                                                   aCGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACt                                                                                                            >  3:568418/1‑55 (MQ=255)
                                                                                                                                   aCGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCt                                               <  3:614008/116‑1 (MQ=255)
                                                                                                                                   aCGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCt                                               >  4:614008/1‑116 (MQ=255)
                                                                                                                                   aCGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCcttct               >  4:631960/1‑148 (MQ=255)
                                                                                                                                   aCGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCcttc                >  1:197870/1‑147 (MQ=255)
                                                                                                                                   aCGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCcttc                <  2:197870/147‑1 (MQ=255)
                                                                                                                                     gcatcaagCGTCGCATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCCACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCttcttt             <  1:755323/141‑1 (MQ=255)
                                                                                                                                        tGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGAttt                                  <  4:58188/124‑1 (MQ=255)
                                                                                                                                        tGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGAttt                                  >  3:58188/1‑124 (MQ=255)
                                                                                                                                               tCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTttcttc   >  3:784896/1‑148 (MQ=255)
                                                                                                                                                cTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTcg  >  1:668695/1‑148 (MQ=255)
                                                                                                                                                  tATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTAt                       <  2:305841/125‑1 (MQ=255)
                                                                                                                                                  tATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTAt                       >  1:305841/1‑125 (MQ=255)
                                                                                                                                                  tATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCttctttct           >  1:634763/1‑137 (MQ=255)
                                                                                                                                                  tATCAGGCCTAAAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTTGAAAATCTGGCGTGCGGATTTCGGCGGCTTAGTCCCttctttct           <  2:634763/137‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCG  >  NC_000913/4457111‑4457402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑