Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,467,149 | T→C | 6.7% | Q33R (CAG→CGG) ‡ | treR ← | trehalose 6‑phosphate‑inducible trehalose regulon transcriptional repressor |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,467,149 | 0 | T | C | 6.7% | 245.9 / 6.6 | 75 | Q33R (CAG→CGG) ‡ | treR | trehalose 6‑phosphate‑inducible trehalose regulon transcriptional repressor |
Reads supporting (aligned to +/- strand): ref base T (35/35); new base C (2/3); total (37/38) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.76e-01 |
GAGATTTTCTGACAACGAATCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCC > NC_000913/4467010‑4467295 | gagaTTTTCTGACAACGAATCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCAcg < 2:1053398/148‑1 (MQ=255) tttCTGACAACGAATCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGCGTGAGCCAGCTCAcccgcg < 4:6319/148‑6 (MQ=255) tCTGACAACGAATCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCttt < 1:1111779/148‑1 (MQ=255) gACAACGAATCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCAcg < 1:1074439/138‑1 (MQ=255) gACAACGAATCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCAcg > 2:1074439/1‑138 (MQ=255) cAACGAATCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCttt > 1:1091921/1‑143 (MQ=255) cAACGAATCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCttt < 2:830156/143‑1 (MQ=255) cAACGAATCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCttt < 2:1091921/143‑1 (MQ=255) cAACGAATCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCttt > 1:830156/1‑143 (MQ=255) cAACGAATCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTa > 3:595021/1‑148 (MQ=255) aTCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCa < 4:319324/128‑1 (MQ=255) aTCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCa > 3:319324/1‑128 (MQ=255) tCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCa < 2:93399/127‑1 (MQ=255) tCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCa > 1:93399/1‑127 (MQ=255) aaCAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCt > 2:184415/1‑110 (MQ=255) aaCAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCt < 1:184415/110‑1 (MQ=255) aaCAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCttt < 2:481316/125‑1 (MQ=255) aaCAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCttt > 1:481316/1‑125 (MQ=255) aaCAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACt < 4:595021/148‑1 (MQ=255) gatgGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGGGG‑GTGAGCTGGCTCCCGCCGCGTTCGTTATTCAGCACCCGGGAAACTGt > 1:638286/1‑144 (MQ=255) gatgGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGt < 2:638286/144‑1 (MQ=255) ggCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGt > 2:711452/1‑141 (MQ=255) ggCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGt < 1:711452/141‑1 (MQ=255) cACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTg < 4:12520/79‑1 (MQ=255) cACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTg > 3:12520/1‑79 (MQ=255) aCGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGc > 1:865900/1‑80 (MQ=255) aCGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGc < 2:865900/80‑1 (MQ=255) aCGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCa > 4:229750/1‑83 (MQ=255) aCGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCa < 3:229750/83‑1 (MQ=255) tAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCCAGCTCAcc > 2:172436/1‑79 (MQ=255) tAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCCAGCTCAcc < 1:172436/80‑2 (MQ=255) cgcgcgcAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCttt < 2:671301/84‑1 (MQ=255) cgcgcgcAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCttt > 1:671301/1‑84 (MQ=255) cgcgcAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGC‑GTGAGCCAGCTCAcccg > 3:42226/1‑74 (MQ=255) cgcgcAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGC‑GTGAGCCAGCTCAcccg < 4:42226/77‑4 (MQ=255) cAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTa > 3:35205/1‑123 (MQ=255) cAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTa < 4:35205/123‑1 (MQ=255) gCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGa > 3:464593/1‑148 (MQ=255) ggAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTa > 2:641512/1‑110 (MQ=255) ggAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTa < 1:641512/110‑1 (MQ=255) ggAAAATCCAGGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTg > 3:985866/1‑127 (MQ=255) ggAAAAACCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGGGCGATATCTTTg < 4:985866/127‑1 (MQ=255) gAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCa > 1:86668/1‑73 (MQ=255) gAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCa < 2:86668/73‑1 (MQ=255) ccATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACt > 1:284070/1‑81 (MQ=255) ccATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACt < 2:284070/81‑1 (MQ=255) ttCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACg < 4:464593/148‑1 (MQ=255) tcaCTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTc < 4:968089/136‑1 (MQ=255) tcaCTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTc > 3:968089/1‑136 (MQ=255) caCTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTaa < 2:736007/141‑1 (MQ=255) caCTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTaa > 1:736007/1‑141 (MQ=255) aCTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGAttt > 2:1129642/1‑120 (MQ=255) aCTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGAttt < 1:1129642/120‑1 (MQ=255) cTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGa > 3:351642/1‑70 (MQ=255) cTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGa < 4:351642/70‑1 (MQ=255) gCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATcc < 2:423107/124‑1 (MQ=255) gCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATcc > 1:423107/1‑124 (MQ=255) tCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGAttt < 2:485171/68‑1 (MQ=255) tCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGAttt > 1:485171/1‑68 (MQ=255) acacGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGAtt < 3:58841/64‑1 (MQ=255) acacGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGAtt > 4:58841/1‑64 (MQ=255) acacGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGttt < 3:3606/141‑1 (MQ=255) acacGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGttt > 4:3606/1‑141 (MQ=255) cacGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTc > 2:554390/1‑101 (MQ=255) cacGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTc < 1:554390/101‑1 (MQ=255) cacGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTAttt > 3:551962/1‑148 (MQ=255) acGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGt > 3:553879/1‑99 (MQ=255) acGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGt < 4:553879/99‑1 (MQ=255) cTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCAt < 2:250280/111‑1 (MQ=255) cTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCAt > 1:250280/1‑111 (MQ=255) g‑gTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGa > 4:1063903/1‑86 (MQ=255) g‑gTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGa < 3:1063903/86‑1 (MQ=255) tGAGCTGGCTCACGCCGCTTTCGTTCTTCAGCACCCGGGAAACTGTAGATTTCCCCAcgccgc > 4:724218/1‑63 (MQ=255) tGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCAcgccgc > 4:21752/1‑63 (MQ=255) tGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCAcgccgc < 3:724218/63‑1 (MQ=255) tGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCAcgccgc < 3:21752/63‑1 (MQ=255) cTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTAccc > 2:350946/1‑148 (MQ=255) | GAGATTTTCTGACAACGAATCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGG‑GTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCC > NC_000913/4467010‑4467295 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |