Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,467,149 T→C 6.7% Q33R (CAG→CGG) ‡ treR ← trehalose 6‑phosphate‑inducible trehalose regulon transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,467,1490TC 6.7% 245.9 / 6.6 75Q33R (CAG→CGG) ‡treRtrehalose 6‑phosphate‑inducible trehalose regulon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (35/35);  new base C (2/3);  total (37/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.76e-01

GAGATTTTCTGACAACGAATCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCC  >  NC_000913/4467010‑4467295
                                                                                                                                            |                                                                                                                                                  
gagaTTTTCTGACAACGAATCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCAcg                                                                                                                                            <  2:1053398/148‑1 (MQ=255)
     tttCTGACAACGAATCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGCGTGAGCCAGCTCAcccgcg                                                                                                                                        <  4:6319/148‑6 (MQ=255)
       tCTGACAACGAATCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCttt                                                                                                                                     <  1:1111779/148‑1 (MQ=255)
          gACAACGAATCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCAcg                                                                                                                                            <  1:1074439/138‑1 (MQ=255)
          gACAACGAATCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCAcg                                                                                                                                            >  2:1074439/1‑138 (MQ=255)
            cAACGAATCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCttt                                                                                                                                     >  1:1091921/1‑143 (MQ=255)
            cAACGAATCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCttt                                                                                                                                     <  2:830156/143‑1 (MQ=255)
            cAACGAATCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCttt                                                                                                                                     <  2:1091921/143‑1 (MQ=255)
            cAACGAATCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCttt                                                                                                                                     >  1:830156/1‑143 (MQ=255)
            cAACGAATCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTa                                                                                                                                >  3:595021/1‑148 (MQ=255)
                  aTCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCa                                                                                                                                              <  4:319324/128‑1 (MQ=255)
                  aTCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCa                                                                                                                                              >  3:319324/1‑128 (MQ=255)
                   tCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCa                                                                                                                                              <  2:93399/127‑1 (MQ=255)
                   tCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCa                                                                                                                                              >  1:93399/1‑127 (MQ=255)
                              aaCAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCt                                                                                                                                                    >  2:184415/1‑110 (MQ=255)
                              aaCAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCt                                                                                                                                                    <  1:184415/110‑1 (MQ=255)
                              aaCAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCttt                                                                                                                                     <  2:481316/125‑1 (MQ=255)
                              aaCAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCttt                                                                                                                                     >  1:481316/1‑125 (MQ=255)
                              aaCAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACt                                                                                                              <  4:595021/148‑1 (MQ=255)
                                    gatgGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGGGGGTGAGCTGGCTCCCGCCGCGTTCGTTATTCAGCACCCGGGAAACTGt                                                                                                            >  1:638286/1‑144 (MQ=255)
                                    gatgGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGt                                                                                                            <  2:638286/144‑1 (MQ=255)
                                       ggCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGt                                                                                                            >  2:711452/1‑141 (MQ=255)
                                       ggCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGt                                                                                                            <  1:711452/141‑1 (MQ=255)
                                                              cACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTg                                                                                                                                                   <  4:12520/79‑1 (MQ=255)
                                                              cACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTg                                                                                                                                                   >  3:12520/1‑79 (MQ=255)
                                                               aCGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGc                                                                                                                                                 >  1:865900/1‑80 (MQ=255)
                                                               aCGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGc                                                                                                                                                 <  2:865900/80‑1 (MQ=255)
                                                               aCGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCa                                                                                                                                              >  4:229750/1‑83 (MQ=255)
                                                               aCGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCa                                                                                                                                              <  3:229750/83‑1 (MQ=255)
                                                                    tAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCCAGCTCAcc                                                                                                                                            >  2:172436/1‑79 (MQ=255)
                                                                    tAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCCAGCTCAcc                                                                                                                                            <  1:172436/80‑2 (MQ=255)
                                                                       cgcgcgcAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCttt                                                                                                                                     <  2:671301/84‑1 (MQ=255)
                                                                       cgcgcgcAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCttt                                                                                                                                     >  1:671301/1‑84 (MQ=255)
                                                                         cgcgcAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGCGTGAGCCAGCTCAcccg                                                                                                                                          >  3:42226/1‑74 (MQ=255)
                                                                         cgcgcAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGCGTGAGCCAGCTCAcccg                                                                                                                                          <  4:42226/77‑4 (MQ=255)
                                                                             cAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTa                                                                                        >  3:35205/1‑123 (MQ=255)
                                                                             cAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTa                                                                                        <  4:35205/123‑1 (MQ=255)
                                                                                 gCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGa                                                           >  3:464593/1‑148 (MQ=255)
                                                                                          ggAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTa                                                                                        >  2:641512/1‑110 (MQ=255)
                                                                                          ggAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTa                                                                                        <  1:641512/110‑1 (MQ=255)
                                                                                          ggAAAATCCAGGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTg                                                                       >  3:985866/1‑127 (MQ=255)
                                                                                          ggAAAAACCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGGGCGATATCTTTg                                                                       <  4:985866/127‑1 (MQ=255)
                                                                                           gAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCa                                                                                                                            >  1:86668/1‑73 (MQ=255)
                                                                                           gAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCa                                                                                                                            <  2:86668/73‑1 (MQ=255)
                                                                                                 ccATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACt                                                                                                              >  1:284070/1‑81 (MQ=255)
                                                                                                 ccATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACt                                                                                                              <  2:284070/81‑1 (MQ=255)
                                                                                                          ttCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACg                                  <  4:464593/148‑1 (MQ=255)
                                                                                                              tcaCTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTc                                          <  4:968089/136‑1 (MQ=255)
                                                                                                              tcaCTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTc                                          >  3:968089/1‑136 (MQ=255)
                                                                                                               caCTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTaa                                    <  2:736007/141‑1 (MQ=255)
                                                                                                               caCTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTaa                                    >  1:736007/1‑141 (MQ=255)
                                                                                                                aCTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGAttt                                                        >  2:1129642/1‑120 (MQ=255)
                                                                                                                aCTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGAttt                                                        <  1:1129642/120‑1 (MQ=255)
                                                                                                                 cTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGa                                                                                                         >  3:351642/1‑70 (MQ=255)
                                                                                                                 cTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGa                                                                                                         <  4:351642/70‑1 (MQ=255)
                                                                                                                   gCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATcc                                                 <  2:423107/124‑1 (MQ=255)
                                                                                                                   gCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATcc                                                 >  1:423107/1‑124 (MQ=255)
                                                                                                                      tCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGAttt                                                                                                      <  2:485171/68‑1 (MQ=255)
                                                                                                                      tCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGAttt                                                                                                      >  1:485171/1‑68 (MQ=255)
                                                                                                                         acacGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGAtt                                                                                                       <  3:58841/64‑1 (MQ=255)
                                                                                                                         acacGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGAtt                                                                                                       >  4:58841/1‑64 (MQ=255)
                                                                                                                         acacGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGttt                          <  3:3606/141‑1 (MQ=255)
                                                                                                                         acacGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGttt                          >  4:3606/1‑141 (MQ=255)
                                                                                                                          cacGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTc                                                                 >  2:554390/1‑101 (MQ=255)
                                                                                                                          cacGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTc                                                                 <  1:554390/101‑1 (MQ=255)
                                                                                                                          cacGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTAttt                  >  3:551962/1‑148 (MQ=255)
                                                                                                                           acGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGt                                                                  >  3:553879/1‑99 (MQ=255)
                                                                                                                           acGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGt                                                                  <  4:553879/99‑1 (MQ=255)
                                                                                                                              cTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCAt                                                   <  2:250280/111‑1 (MQ=255)
                                                                                                                              cTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCAt                                                   >  1:250280/1‑111 (MQ=255)
                                                                                                                                    ggTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGa                                                                      >  4:1063903/1‑86 (MQ=255)
                                                                                                                                    ggTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGa                                                                      <  3:1063903/86‑1 (MQ=255)
                                                                                                                                       tGAGCTGGCTCACGCCGCTTTCGTTCTTCAGCACCCGGGAAACTGTAGATTTCCCCAcgccgc                                                                                           >  4:724218/1‑63 (MQ=255)
                                                                                                                                       tGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCAcgccgc                                                                                           >  4:21752/1‑63 (MQ=255)
                                                                                                                                       tGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCAcgccgc                                                                                           <  3:724218/63‑1 (MQ=255)
                                                                                                                                       tGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCAcgccgc                                                                                           <  3:21752/63‑1 (MQ=255)
                                                                                                                                           cTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTAccc  >  2:350946/1‑148 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
GAGATTTTCTGACAACGAATCCAGACGGGTAACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCC  >  NC_000913/4467010‑4467295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: