Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,470,958 G→T 16.2% intergenic (‑45/+28) ridA ← / ← pyrI enamine/imine deaminase, reaction intermediate detoxification/aspartate carbamoyltransferase, regulatory subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,470,9580GT16.2% 124.3 / 14.2 43intergenic (‑45/+28)ridA/pyrIenamine/imine deaminase, reaction intermediate detoxification/aspartate carbamoyltransferase, regulatory subunit
Reads supporting (aligned to +/- strand):  ref base G (19/17);  new base T (3/4);  total (22/21)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

GGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGAT  >  NC_000913/4470814‑4471105
                                                                                                                                                |                                                                                                                                                   
ggATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAg                                                                                                                                                  <  1:543853/148‑1 (MQ=255)
                                       cGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACttt                                                                                                                                         >  4:629064/1‑118 (MQ=255)
                                       cGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACttt                                                                                                                                         <  3:629064/118‑1 (MQ=255)
                                            tGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGACt                                                                                                                                           <  1:240981/111‑1 (MQ=38)
                                            tGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGACt                                                                                                                                           >  2:240981/1‑111 (MQ=38)
                                                      gACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGACt                                                                                                                                           >  3:325521/1‑101 (MQ=38)
                                                      gACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGACt                                                                                                                                           <  4:325521/101‑1 (MQ=38)
                                                            tAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCaattaa                                                                                                                      <  4:502544/116‑1 (MQ=255)
                                                            tAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCaattaa                                                                                                                      >  3:502544/1‑116 (MQ=255)
                                                                           tttCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGcc                                                                                                                                                <  1:490487/75‑1 (MQ=37)
                                                                           tttCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGcc                                                                                                                                                >  2:490487/1‑75 (MQ=37)
                                                                           tttCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGc                                                                                                                                                 <  1:1104253/74‑1 (MQ=37)
                                                                                 tCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTtta                                                                                                                                       <  1:977226/78‑1 (MQ=255)
                                                                                 tCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTtta                                                                                                                                       >  2:977226/1‑78 (MQ=255)
                                                                                 tCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTCGCTCATTATAGGGAGCCAGa                                                                                                                                             >  3:645509/1‑72 (MQ=255)
                                                                                 tCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTCGCTCATTATAGGGAGCCAGa                                                                                                                                             <  4:645509/72‑1 (MQ=255)
                                                                                                 cATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACt                                                                                                                                           <  4:1045738/58‑1 (MQ=255)
                                                                                                 cATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACt                                                                                                                                           >  3:1045738/1‑58 (MQ=255)
                                                                                                             tttATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGtttt                                           >  3:518309/1‑142 (MQ=255)
                                                                                                             tttATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGtttt                                           <  4:518309/142‑1 (MQ=255)
                                                                                                                                  cTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTAt                                                                                                   <  4:807352/65‑1 (MQ=255)
                                                                                                                                  cTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTAt                                                                                                   >  3:807352/1‑65 (MQ=255)
                                                                                                                                    ggCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCAt                                                                      >  3:230650/1‑92 (MQ=255)
                                                                                                                                    ggCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCAt                                                                      <  4:230650/92‑1 (MQ=255)
                                                                                                                                      cTCATTATAGGGAGCCAGACTTTTATTACCAACCGCaattaat                                                                                                                     <  1:342980/43‑1 (MQ=255)
                                                                                                                                      cTCATTATAGGGAGCCAGACTTTTATTACCAACCGCaattaat                                                                                                                     >  2:342980/1‑43 (MQ=255)
                                                                                                                                      cTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTgg                           >  2:356216/1‑133 (MQ=255)
                                                                                                                                      cTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTgg                           <  1:356216/133‑1 (MQ=255)
                                                                                                                                        cATTATAGGGATCCAGCCTTTTATAATCAACCACAATTAATTGGCCTGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGAGTTTTCGCACGGCTAAGCTGGATGAAACCGGTTCGGCa          <  1:282913/148‑1 (MQ=255)
                                                                                                                                        cATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCtttt                                                                                     >  4:779151/1‑73 (MQ=255)
                                                                                                                                        cATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCtttt                                                                                     <  3:779151/73‑1 (MQ=255)
                                                                                                                                        cATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGc                                                                        <  4:672848/86‑1 (MQ=255)
                                                                                                                                        cATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCa          >  2:9969/1‑148 (MQ=255)
                                                                                                                                        cATTATAGGGAGCCAGACATTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGc                                                                        >  3:672848/1‑86 (MQ=255)
                                                                                                                                         attattGGGAGCCAGACATTTATTACCAACCGCAATTAATTGGCCAACACCACATTATGGAAAAACTCTTTTGCACAGTATTTGCATTTGAGCGCGATATCATTTGCACGTTTTCGCACGGCAAAGCTGTATGATACCGGTTCGGCAt         >  2:282913/1‑148 (MQ=255)
                                                                                                                                          ttATAGGTAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGat                                                           >  4:94214/1‑97 (MQ=255)
                                                                                                                                          ttATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGat                                                           <  3:94214/97‑1 (MQ=255)
                                                                                                                                          ttATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATg        >  3:733519/1‑148 (MQ=255)
                                                                                                                                           tataGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTc                                          >  3:731830/1‑113 (MQ=255)
                                                                                                                                           tataGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTc                                          <  4:731830/113‑1 (MQ=255)
                                                                                                                                           tataGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCgg                 >  4:399845/1‑138 (MQ=255)
                                                                                                                                           tataGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCgg                 <  3:399845/138‑1 (MQ=255)
                                                                                                                                           tataGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATgg       >  1:1003661/1‑148 (MQ=255)
                                                                                                                                                ggAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTCCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGAt  >  3:279512/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGAT  >  NC_000913/4470814‑4471105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: