Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,484,395 A→C 5.3% intergenic (‑115/+45) holC ← / ← pepA DNA polymerase III, chi subunit/multifunctional aminopeptidase A: a cyteinylglycinase, transcription regulator and site‑specific recombination factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,484,3950AC 5.3% 275.1 / 6.7 75intergenic (‑115/+45)holC/pepADNA polymerase III, chi subunit/multifunctional aminopeptidase A: a cyteinylglycinase, transcription regulator and site‑specific recombination factor
Reads supporting (aligned to +/- strand):  ref base A (36/35);  new base C (2/2);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

ATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGG  >  NC_000913/4484248‑4484540
                                                                                                                                                   |                                                                                                                                                 
aTTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATaa                                                                                                                                                   <  4:117740/148‑1 (MQ=255)
        gAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATGCGGCGtgaa                                                                                                                                           >  3:42432/1‑145 (MQ=255)
        gAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGCCGGATGCGGCGtt                                                                                                                                             <  2:66383/146‑1 (MQ=255)
        gAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGCCGGATGCGGCGtt                                                                                                                                             >  1:66383/1‑146 (MQ=255)
              aGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATGCGGCGTGAAcgcctt                                                                                                                                     <  4:42432/148‑3 (MQ=255)
                    tCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCgg                                                                                                                               >  4:399796/1‑148 (MQ=255)
                          ttttCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGAGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAAc                                                                                                                        <  4:928026/148‑1 (MQ=255)
                                     ggggCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAg                                                                                                                      >  3:918842/1‑140 (MQ=255)
                                     ggggCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAg                                                                                                                      <  4:918842/140‑1 (MQ=255)
                                                 ggtggATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTAct                                                                                                  <  3:399796/148‑1 (MQ=255)
                                                        ttAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTc                                                                                                                                            >  4:1053758/1‑99 (MQ=255)
                                                        ttAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTc                                                                                                                                            <  3:1053758/99‑1 (MQ=255)
                                                         tAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCg                                                                                          >  2:192780/1‑148 (MQ=255)
                                                               cGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAAc                                                                                    >  4:722000/1‑148 (MQ=255)
                                                                 cATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGc                                                                                                                                                <  2:189812/86‑1 (MQ=255)
                                                                 cATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGc                                                                                                                                                >  1:189812/1‑86 (MQ=255)
                                                                  aTCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCa                                                                                 >  1:750297/1‑148 (MQ=255)
                                                                     aGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAgcg                                                                              <  2:750297/148‑1 (MQ=255)
                                                                       gTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGc                                                                                                                              <  1:755556/98‑1 (MQ=255)
                                                                       gTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGc                                                                                                                              >  2:755556/1‑98 (MQ=255)
                                                                        tataCACCGCAGCGCCTGATGCGACGCTGTTGTGTATTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCgg                                                                           >  4:1078708/1‑148 (MQ=255)
                                                                         ataCACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTCGGTCAGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGt                                                                          <  3:1078708/148‑1 (MQ=255)
                                                                         ataCACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGt                                                                          >  4:377351/1‑148 (MQ=255)
                                                                            cacCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGt                                                                                                                                              >  1:693524/1‑77 (MQ=255)
                                                                            cacCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGt                                                                                                                                              <  2:693524/77‑1 (MQ=255)
                                                                              ccGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCaa                                                                                                       >  3:133157/1‑114 (MQ=255)
                                                                              ccGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCaa                                                                                                       <  4:133157/114‑1 (MQ=255)
                                                                              ccGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCGGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAAc                                                                     <  1:192780/148‑1 (MQ=255)
                                                                               cGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACa                                                                    <  3:377351/148‑1 (MQ=255)
                                                                                         gATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGc                                                          >  1:297301/1‑148 (MQ=255)
                                                                                            gCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAAcc                                                                                   >  2:562675/1‑120 (MQ=255)
                                                                                            gCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAAcc                                                                                   <  1:562675/120‑1 (MQ=255)
                                                                                               aCGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCaa                                                    <  3:722000/148‑1 (MQ=255)
                                                                                                cGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAAc                                                                     >  1:195522/1‑130 (MQ=255)
                                                                                                cGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAAc                                                                     <  2:195522/130‑1 (MQ=255)
                                                                                                 gCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACg                                                  <  2:297301/148‑1 (MQ=255)
                                                                                                    gttgtGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAAtt                                                                                                     >  2:129562/1‑94 (MQ=255)
                                                                                                    gttgtGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAAtt                                                                                                     <  1:129562/94‑1 (MQ=255)
                                                                                                    gttgtGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAAtt                                                                                                     >  4:681715/1‑94 (MQ=255)
                                                                                                    gttgtGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAAtt                                                                                                     <  3:681715/94‑1 (MQ=255)
                                                                                                           cTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTa                                                                                                    >  3:644193/1‑88 (MQ=255)
                                                                                                           cTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTa                                                                                                    <  4:644193/88‑1 (MQ=255)
                                                                                                           cTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCGCGCTGGGTTTAAcg                                                                    <  2:62758/120‑2 (MQ=37)
                                                                                                           cTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGa                                                                 >  2:1002677/1‑123 (MQ=255)
                                                                                                           cTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGa                                                                 <  1:1002677/123‑1 (MQ=255)
                                                                                                             tATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATc                                                                                                                                  >  3:1081758/1‑56 (MQ=255)
                                                                                                             tATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATc                                                                                                                                  <  4:1081758/56‑1 (MQ=255)
                                                                                                             tATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATACCAGCCTTg                                                                                                                 <  4:982300/73‑1 (MQ=255)
                                                                                                             tATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTg                                                                                                                 >  3:982300/1‑73 (MQ=255)
                                                                                                                cAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTaaa                                                                                     >  2:1112836/1‑98 (MQ=255)
                                                                                                                cAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTaaa                                                                                     <  1:1112836/98‑1 (MQ=255)
                                                                                                                cAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACtgt                                                            <  3:291707/123‑1 (MQ=255)
                                                                                                                cAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACtgt                                                            >  4:291707/1‑123 (MQ=255)
                                                                                                                  ggCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTaa                                                                                      <  2:711207/95‑1 (MQ=255)
                                                                                                                  ggCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTaa                                                                                      >  1:711207/1‑95 (MQ=255)
                                                                                                                       aCGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGt                                  >  3:199841/1‑142 (MQ=255)
                                                                                                                       aCGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGt                                  <  4:199841/142‑1 (MQ=255)
                                                                                                                       aCGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGcc                            >  1:502625/1‑148 (MQ=255)
                                                                                                                          aGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCttt                         <  2:502625/148‑1 (MQ=255)
                                                                                                                            ttCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCgg                                   >  3:637718/1‑136 (MQ=255)
                                                                                                                            ttCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCgg                                   <  4:637718/136‑1 (MQ=255)
                                                                                                                             tCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGt                                  >  1:320179/1‑136 (MQ=255)
                                                                                                                             tCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGt                                  <  2:320179/136‑1 (MQ=255)
                                                                                                                             tCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTg                       >  4:488035/1‑147 (MQ=255)
                                                                                                                             tCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTg                       <  3:488035/147‑1 (MQ=255)
                                                                                                                              cAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCt                     >  4:750093/1‑148 (MQ=255)
                                                                                                                                 tGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTa                  >  4:909895/1‑148 (MQ=255)
                                                                                                                                 tGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTa                  <  3:909895/148‑1 (MQ=255)
                                                                                                                                            tCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGa                                                                 >  2:633525/1‑90 (MQ=255)
                                                                                                                                            tCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGa                                                                 <  1:633525/90‑1 (MQ=255)
                                                                                                                                            tCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGga                                          >  2:492513/1‑113 (MQ=255)
                                                                                                                                            tCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGga                                          <  1:492513/113‑1 (MQ=255)
                                                                                                                                            tCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCa       >  4:331945/1‑148 (MQ=255)
                                                                                                                                              ggATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGga                                          <  4:212581/111‑1 (MQ=255)
                                                                                                                                              ggATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGga                                          >  3:212581/1‑111 (MQ=255)
                                                                                                                                                 tAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCgg  >  2:870537/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGG  >  NC_000913/4484248‑4484540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: