Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,565,345 G→C 11.5% A169G (GCC→GGC)  yjiM ← putative 2‑hydroxyglutaryl‑CoA dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,565,3450GC11.5% 170.0 / 10.3 52A169G (GCC→GGC) yjiMputative 2‑hydroxyglutaryl‑CoA dehydratase
Reads supporting (aligned to +/- strand):  ref base G (23/23);  new base C (3/3);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAA  >  NC_000913/4565205‑4565489
                                                                                                                                            |                                                                                                                                                
tCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATcgcg                                                                                                                                           >  2:865242/1‑148 (MQ=255)
                         ggTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGc                                                                                                                  >  3:446272/1‑148 (MQ=255)
                                   tAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGtt                                                                                                        <  4:446272/148‑1 (MQ=255)
                                   tAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGtt                                                                                                        <  1:865242/148‑1 (MQ=255)
                                            ttCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGttc                                                                                                       <  3:598606/140‑1 (MQ=255)
                                            ttCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGttc                                                                                                       >  4:598606/1‑140 (MQ=255)
                                                         tGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCa                                                                                                                                    <  2:785831/98‑1 (MQ=255)
                                                         tGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCa                                                                                                                                    >  1:785831/1‑98 (MQ=255)
                                                              cTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTAc                                                                                                 >  1:207873/1‑128 (MQ=255)
                                                              cTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTAc                                                                                                 <  2:207873/128‑1 (MQ=255)
                                                                  gcgcCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCAtctc                                                                         >  3:648283/1‑148 (MQ=255)
                                                                   cgcCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCag                                                                                                                                        <  1:1120105/84‑1 (MQ=255)
                                                                   cgcCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCag                                                                                                                                        >  2:1120105/1‑84 (MQ=255)
                                                                         aggaTTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCAtctc                                                                         <  4:555433/141‑1 (MQ=255)
                                                                         aggaTTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCAtctc                                                                         >  3:555433/1‑141 (MQ=255)
                                                                             tttAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATa                                                              >  1:742061/1‑148 (MQ=255)
                                                                              ttAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATaa                                                             <  4:648283/148‑1 (MQ=255)
                                                                                    gCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAacgcacg                                                       >  1:824429/1‑148 (MQ=255)
                                                                                         aGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGttc                                                                                                       <  4:753835/95‑1 (MQ=255)
                                                                                         aGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGttc                                                                                                       >  3:753835/1‑95 (MQ=255)
                                                                                               tAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAAtct                                                                                                                       <  1:886477/73‑1 (MQ=255)
                                                                                               tAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAAtct                                                                                                                       >  2:886477/1‑73 (MQ=255)
                                                                                                aaaaaTTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCa                                                                                      >  4:273927/1‑105 (MQ=255)
                                                                                                aaaaaTTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCa                                                                                      <  3:273927/105‑1 (MQ=255)
                                                                                                aaaaaTTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTc                                           >  3:900748/1‑148 (MQ=255)
                                                                                                 aaaaTTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGtt                                                                                                        >  4:767132/1‑86 (MQ=255)
                                                                                                 aaaaTTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGtt                                                                                                        <  3:767132/86‑1 (MQ=255)
                                                                                                 aaaaTTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGttt                                                                                            <  2:715386/98‑1 (MQ=255)
                                                                                                 aaaaTTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGttt                                                                                            >  1:715386/1‑98 (MQ=255)
                                                                                                           aGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGt                                <  2:742061/148‑1 (MQ=255)
                                                                                                                  gACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCaa                         <  4:900748/148‑1 (MQ=255)
                                                                                                                         gcgGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCTTc                  <  2:824429/148‑1 (MQ=255)
                                                                                                                           ggTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAgcg                                                                                  <  4:133381/82‑1 (MQ=255)
                                                                                                                           ggTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAgcg                                                                                  >  3:133381/1‑82 (MQ=255)
                                                                                                                             tttttCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGttttt                                                                                          <  4:1125325/72‑1 (MQ=255)
                                                                                                                             tttttCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGttttt                                                                                          >  3:1125325/1‑72 (MQ=255)
                                                                                                                             tttttCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGt                                >  1:255770/1‑130 (MQ=255)
                                                                                                                             tttttCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGt                                <  2:255770/130‑1 (MQ=255)
                                                                                                                                  ctgcgcGATGCCATTGCGCTGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTa                                       <  1:190759/116‑1 (MQ=255)
                                                                                                                                  ctgcgcGATGCCATTGCGCTGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATcac                >  1:641772/3‑141 (MQ=255)
                                                                                                                                  ctgcgcGATGCCATTGCGCTGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATcac                <  2:641772/139‑1 (MQ=255)
                                                                                                                                  ctgcgcGATGCCATTGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAGCa         >  1:867786/3‑148 (MQ=255)
                                                                                                                                  cAGCGCGATGCCATTGCGCTGAGCATCTTCTCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTGCATAACGCACGCGAGGCATCGTCCTTa                                       >  2:190759/1‑118 (MQ=255)
                                                                                                                                     cgcAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAg                                                                                    <  3:944915/70‑1 (MQ=255)
                                                                                                                                     cgcAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGAGCCCAAAACGTTCTTCTACCGTTTTTTGCAAg                                                                                    >  4:944915/1‑70 (MQ=255)
                                                                                                                                        aaTGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCAt                                               >  1:285167/1‑104 (MQ=255)
                                                                                                                                        aaTGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCAt                                               <  2:285167/104‑1 (MQ=255)
                                                                                                                                         aTGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCtt                                                                    <  3:711396/82‑1 (MQ=255)
                                                                                                                                         aTGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCtt                                                                    >  4:711396/1‑82 (MQ=255)
                                                                                                                                         aTGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGtt                               >  2:424645/1‑119 (MQ=255)
                                                                                                                                         aTGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGtt                               <  1:424645/119‑1 (MQ=255)
                                                                                                                                         aTGCCATTGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAGCAGGCTTaa  <  2:867786/148‑1 (MQ=255)
                                                                                                                                           ggCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGc                                                                      >  3:827074/1‑78 (MQ=255)
                                                                                                                                           ggCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGc                                                                      <  4:827074/78‑1 (MQ=255)
                                                                                                                                            gCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGt                                <  2:720008/115‑1 (MQ=255)
                                                                                                                                            gCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGt                                >  1:720008/1‑115 (MQ=255)
                                                                                                                                            gCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTaa  <  4:513683/145‑1 (MQ=255)
                                                                                                                                            gCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTaa  >  3:513683/1‑145 (MQ=255)
                                                                                                                                            |                                                                                                                                                
TCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAA  >  NC_000913/4565205‑4565489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑