Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,612,329 | A→G | 33.2% | intergenic (+40/‑82) | ytjA → / → yjjU | uncharacterized protein/putative patatin‑like family phospholipase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,612,329 | 0 | A | G | 33.2% | 16.9 / 7.2 | 15 | intergenic (+40/‑82) | ytjA/yjjU | uncharacterized protein/putative patatin‑like family phospholipase |
Reads supporting (aligned to +/- strand): ref base A (5/5); new base G (2/3); total (7/8) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 |
TTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGT > NC_000913/4612263‑4612453 | ttGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACt > 4:998669/1‑74 (MQ=255) ttGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACt < 3:998669/74‑1 (MQ=255) aCCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGaataat > 1:374439/1‑79 (MQ=255) aCCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGaataat < 2:374439/79‑1 (MQ=255) aGCCAGTCCGCTGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCaga > 1:545504/1‑90 (MQ=255) aGCCAGTCCGCTGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCaga < 2:545504/90‑1 (MQ=255) gCCAGTCCGCTGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTc > 3:30047/1‑67 (MQ=38) gCCAGTCCGCTGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTc < 4:30047/67‑1 (MQ=38) ccAGTCCGCTGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTa < 3:611400/51‑1 (MQ=25) tCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCt > 3:301529/1‑111 (MQ=255) tCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCt < 4:301529/111‑1 (MQ=255) ccAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCAt > 3:632092/1‑55 (MQ=255) ccAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCAt < 4:632092/55‑1 (MQ=255) ccAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGt < 2:970037/127‑1 (MQ=255) ccAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGt > 1:970037/1‑127 (MQ=255) aGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCag > 3:385319/1‑80 (MQ=255) aGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCag < 4:385319/80‑1 (MQ=255) | TTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGT > NC_000913/4612263‑4612453 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |