Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 60,263 C→T 5.1% K28K (AAG→AAA rluA ← dual specificity 23S rRNA pseudouridine(746), tRNA pseudouridine(32) synthase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091360,2630CT 5.1% 458.8 / 4.7 132K28K (AAG→AAArluAdual specificity 23S rRNA pseudouridine(746), tRNA pseudouridine(32) synthase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base C (62/63);  new base T (4/3);  total (66/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 132 total aligned reads displayed.

GGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCA  >  NC_000913/60119‑60402
                                                                                                                                                |                                                                                                                                           
ggTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCttg                                                                                                                                          <  2:991946/148‑1 (MQ=255)
    aGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGa                                                                                                                                      >  1:203131/1‑148 (MQ=255)
     gcgcCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAGCCGAGCGGTttgttg                                                                                                                                       >  4:95064/1‑146 (MQ=255)
     gcgcCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAGCCGAGCGGTttgttg                                                                                                                                       <  3:95064/146‑1 (MQ=255)
                aTCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAATCCGCTCGGCTTGTTGACCACCATAatat                                                                                                                          >  4:730501/1‑148 (MQ=255)
                 tCACGCCGCTCGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAatat                                                                                                                          <  1:1082515/147‑1 (MQ=255)
                 tCACGCCGCTCGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAatat                                                                                                                          >  2:1082515/1‑147 (MQ=255)
                     gccgcTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCa                                                                                                                     <  4:21478/148‑1 (MQ=255)
                      ccgcTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCAt                                                                                                                    <  3:730501/148‑1 (MQ=255)
                      ccgcTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCAt                                                                                                                    <  1:781733/148‑1 (MQ=255)
                         cTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCt                                                                                                                 >  1:91866/1‑148 (MQ=255)
                                 cATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATcc                                                                                                                  >  4:839329/1‑139 (MQ=255)
                                 cATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATcc                                                                                                                  <  3:839329/139‑1 (MQ=255)
                                 cATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAgg                                                                                                         <  2:91866/148‑1 (MQ=255)
                                     tCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATaa                                                                                                     <  2:435462/148‑1 (MQ=255)
                                      ccAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCttgt                                                                                                                                         >  4:1108080/1‑111 (MQ=255)
                                      ccAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATa                                                                                                             >  3:133548/1‑139 (MQ=255)
                                      ccAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATa                                                                                                             <  4:133548/139‑1 (MQ=255)
                                        aGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATcc                                                                                                                  <  3:868039/132‑1 (MQ=255)
                                        aGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTg                                                                                                                >  3:172864/1‑134 (MQ=255)
                                          aCGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCttg                                                                                                                                          >  4:791074/1‑106 (MQ=255)
                                          aCGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACa                                                                                                           >  3:486618/1‑137 (MQ=255)
                                          aCGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACa                                                                                                           <  4:486618/137‑1 (MQ=255)
                                              tGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAgg                                                                                            <  2:203131/148‑1 (MQ=255)
                                                  ccGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCata                                                                                                                              >  3:799717/1‑110 (MQ=255)
                                                  ccGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCata                                                                                                                              <  4:799717/110‑1 (MQ=255)
                                                  ccGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCAt                                                                                                                    <  3:709342/120‑1 (MQ=255)
                                                  ccGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCAt                                                                                                                    <  2:576185/120‑1 (MQ=255)
                                                  ccGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATa                                                                                                             >  3:306640/1‑127 (MQ=255)
                                                  ccGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATa                                                                                                             <  4:306640/127‑1 (MQ=255)
                                                  ccGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTc                                                                                        >  2:169285/1‑148 (MQ=255)
                                                             tgcGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGAGCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATc                                                                                                                   >  1:965826/1‑110 (MQ=255)
                                                             tgcGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATc                                                                                                                   <  2:965826/110‑1 (MQ=255)
                                                               cGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTg                                                                           >  4:82493/1‑148 (MQ=255)
                                                                  aTAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAg                                                                        >  3:458679/1‑148 (MQ=255)
                                                                   tAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGaccacc                                                                                                                                 >  1:576247/1‑90 (MQ=255)
                                                                   tAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGaccacc                                                                                                                                 <  2:576247/90‑1 (MQ=255)
                                                                   tAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGt                                                                       <  3:250064/148‑1 (MQ=255)
                                                                     aTCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATg                                                                                                                         <  4:635326/96‑1 (MQ=255)
                                                                     aTCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATg                                                                                                                         >  3:635326/1‑96 (MQ=255)
                                                                       cACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCttgttg                                                                                                                                       <  3:800192/80‑1 (MQ=255)
                                                                       cACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGt                                                                          <  2:540543/141‑1 (MQ=255)
                                                                       cACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGt                                                                          >  1:540543/1‑141 (MQ=255)
                                                                       cACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTc                                                                   <  1:169285/148‑1 (MQ=255)
                                                                             gAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGa                                                                                                               <  2:751828/98‑1 (MQ=255)
                                                                             gAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGa                                                                              >  4:486125/1‑131 (MQ=255)
                                                                              aaTGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCAt                                                                                                                    >  1:890753/1‑92 (MQ=255)
                                                                                  cgcgTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGttt                                                                     <  4:862449/135‑1 (MQ=255)
                                                                                         tcaCGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTc                                                 >  3:583823/1‑148 (MQ=255)
                                                                                          caCGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCAtttt                                                       >  1:642750/1‑141 (MQ=255)
                                                                                          caCGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTcc                                                >  2:155676/1‑148 (MQ=255)
                                                                                              cTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCttg                                                                                                                                          <  3:694062/54‑1 (MQ=255)
                                                                                              cTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCttg                                                                                                                                          >  4:694062/1‑54 (MQ=255)
                                                                                              cTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCt                                                                                                                                            <  4:652079/52‑1 (MQ=255)
                                                                                                gTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTc                                                 >  4:960806/1‑141 (MQ=255)
                                                                                                gTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACt                                          >  3:966660/1‑148 (MQ=255)
                                                                                                 tCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTgg                                                                               <  4:391487/110‑1 (MQ=255)
                                                                                                  cTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCttgttg                                                                                                                                       <  1:613422/53‑1 (MQ=255)
                                                                                                  cTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCttgttg                                                                                                                                       >  2:613422/1‑53 (MQ=255)
                                                                                                    ttGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTg                                                                                                                <  4:476210/74‑1 (MQ=255)
                                                                                                    ttGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTg                                                                                                                >  3:476210/1‑74 (MQ=255)
                                                                                                    ttGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATccc                                                             <  1:644793/125‑1 (MQ=255)
                                                                                                    ttGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATccc                                                             >  2:644793/1‑125 (MQ=255)
                                                                                                     tgtgCTCTTCCAGACGACCCGGCACTGACAACAAACCGCACGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGc                                     <  1:155676/148‑1 (MQ=255)
                                                                                                          tctTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTAc                                >  1:1010072/1‑148 (MQ=255)
                                                                                                           ctTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGTATCAGGAtg                                                                                                      <  1:85395/77‑2 (MQ=255)
                                                                                                           ctTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGTATCAGGAtg                                                                                                      >  2:85395/1‑76 (MQ=255)
                                                                                                           ctTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACa                               >  1:166927/1‑148 (MQ=255)
                                                                                                             tCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAAc                             >  3:21904/1‑148 (MQ=255)
                                                                                                             tCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAAc                             >  2:594469/1‑148 (MQ=255)
                                                                                                               cAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGa                           <  2:166927/148‑1 (MQ=255)
                                                                                                               cAGACGACCCGGCACTGACAACAAACCGCTCGGCATGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGCTCCTGCGGTGGCTTGCAGTTTGCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAAccg                           >  2:34770/1‑146 (MQ=255)
                                                                                                                aGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGAt                          <  2:1010072/148‑1 (MQ=255)
                                                                                                                aGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGAt                          <  1:34770/148‑1 (MQ=255)
                                                                                                                  acgacCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCgg                                                    <  1:391792/120‑1 (MQ=255)
                                                                                                                  acgacCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCgg                                                    >  2:391792/1‑120 (MQ=255)
                                                                                                                  acgacCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATg                                      >  3:881159/1‑134 (MQ=255)
                                                                                                                  acgacCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATg                                      <  4:881159/134‑1 (MQ=255)
                                                                                                                   cgacCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCaa                       >  2:796170/1‑148 (MQ=255)
                                                                                                                   cgacCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCaa                       <  4:21904/148‑1 (MQ=255)
                                                                                                                     acCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAAc                     >  4:208988/1‑148 (MQ=255)
                                                                                                                       ccGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGc                   <  1:590256/148‑1 (MQ=255)
                                                                                                                        cGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATc                         <  2:882168/141‑1 (MQ=255)
                                                                                                                        cGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCa                  >  1:1101179/1‑148 (MQ=255)
                                                                                                                        cGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCa                  <  1:796170/148‑1 (MQ=255)
                                                                                                                              tGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCAt            <  2:1101179/148‑1 (MQ=255)
                                                                                                                               gACAACAAACCGCTCGGCTTGTTGACCACCATACTATGGTCATCCTGATAc                                                                                                            >  3:1114338/1‑51 (MQ=255)
                                                                                                                               gACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCg                                    >  2:630769/1‑123 (MQ=255)
                                                                                                                               gACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTAATAc                                                                                                            <  4:1114338/51‑1 (MQ=255)
                                                                                                                                 caacaaGCCGAGCGGTTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATc                                                               >  1:25245/1‑94 (MQ=255)
                                                                                                                                 caacaaGCCGAGCGGTTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATc                                                               <  2:25245/94‑1 (MQ=255)
                                                                                                                                 caacaaACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCAtttt                                                       <  2:1109061/102‑1 (MQ=255)
                                                                                                                                   acaaACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCAtttt                                                       >  2:999107/1‑100 (MQ=255)
                                                                                                                                    caaACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGAc      >  1:1094024/1‑148 (MQ=255)
                                                                                                                                     aaaCCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGa                           <  1:201691/126‑1 (MQ=255)
                                                                                                                                     aaaCCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGa                           >  2:201691/1‑126 (MQ=255)
                                                                                                                                      aaCCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGtt                                                                      <  2:649034/82‑1 (MQ=255)
                                                                                                                                      aaCCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGtt                                                                      >  1:649034/1‑82 (MQ=255)
                                                                                                                                        ccGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGc                   >  4:401999/1‑131 (MQ=255)
                                                                                                                                        ccGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGc                   <  3:401999/131‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                           
GGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCA  >  NC_000913/60119‑60402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: