| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 504,822 | G→C | 5.3% | intergenic (‑126/‑92) | fsr ← / → ushA | putative fosmidomycin efflux system protein/bifunctional UDP‑sugar hydrolase/5'‑nucleotidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 504,822 | 0 | G | C | 5.3% | 273.8 / 5.6 | 76 | intergenic (‑126/‑92) | fsr/ushA | putative fosmidomycin efflux system protein/bifunctional UDP‑sugar hydrolase/5'‑nucleotidase |
| Reads supporting (aligned to +/- strand): ref base G (36/36); new base C (2/2); total (38/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.72e-01 | |||||||||||
CACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTAC > NC_000913/504705‑504963 | cACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGa < 3:60738/119‑1 (MQ=255)cACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGa > 4:60738/1‑119 (MQ=255) cTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTAt < 4:804355/128‑1 (MQ=255) cTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTAt > 3:804355/1‑128 (MQ=255) tttttCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGCATCCAGAttaga > 4:882657/1‑124 (MQ=255) tttttCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGCATCCAGAttaga < 3:882657/127‑4 (MQ=255) gATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTc > 2:28330/1‑135 (MQ=255) gATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTc < 1:28330/135‑1 (MQ=255) gATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGt > 1:38158/1‑148 (MQ=255) gTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTc < 4:827072/114‑1 (MQ=255) gTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTc > 3:827072/1‑114 (MQ=255) aGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGa > 4:889030/1‑95 (MQ=255) aGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGa < 3:889030/95‑1 (MQ=255) tCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCa > 2:1113529/1‑148 (MQ=255) cTTATTTGTGATTATTACCAGGCTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCaa > 3:370392/1‑148 (MQ=255) tATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGa > 1:324406/1‑106 (MQ=255) tATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGa < 2:324406/106‑1 (MQ=255) tATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATa > 4:52351/1‑135 (MQ=255) tATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATa < 3:52351/135‑1 (MQ=255) gATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCa > 1:1025569/1‑126 (MQ=255) gATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCa < 2:1025569/126‑1 (MQ=255) gATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGaa < 2:38158/148‑1 (MQ=255) tattaCCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCa < 1:10919/91‑1 (MQ=255) tattaCCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCa > 2:10919/1‑91 (MQ=255) aGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTcgc < 3:997940/112‑1 (MQ=255) aGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTcgc > 4:997940/1‑112 (MQ=255) aaCATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTg > 1:660664/1‑94 (MQ=255) aaCATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTg < 2:660664/94‑1 (MQ=255) aCCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGt < 1:1113529/148‑1 (MQ=255) aCCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGt > 3:491938/1‑148 (MQ=255) ccTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCa > 2:267513/1‑117 (MQ=255) ccTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCa < 1:267513/117‑1 (MQ=255) tggaTGCGTCGTCTGAAGGGAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATa > 2:220732/4‑105 (MQ=255) tGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATa < 1:220732/105‑1 (MQ=255) tGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACg < 3:444170/120‑1 (MQ=255) tGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACg > 4:444170/1‑120 (MQ=255) tATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAGCACGTTGAAATCAGGTCAGGGAGAGAAGTATGaa < 4:370392/148‑1 (MQ=255) cgtcgtCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGgagaga > 4:203977/1‑136 (MQ=255) cgtcgtCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGgagaga < 3:203977/136‑1 (MQ=255) cTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTatgat > 3:838293/1‑82 (MQ=255) cTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTatgat < 4:838293/82‑1 (MQ=255) gaaggaagTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAAtca < 1:824627/115‑1 (MQ=255) gaaggaagTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAAtca > 2:824627/1‑115 (MQ=255) gaaggaagTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAAt < 2:58098/113‑1 (MQ=255) gaaggaagTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAAt > 1:58098/1‑113 (MQ=255) aaggaagTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAAtcag < 2:37919/115‑1 (MQ=255) aaggaagTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAAtcag > 1:37919/1‑115 (MQ=255) aggaagTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCa < 1:138133/87‑1 (MQ=255) aggaagTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCa > 2:138133/1‑87 (MQ=255) aagTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGt < 2:157671/82‑1 (MQ=255) aagTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGt > 1:157671/1‑82 (MQ=255) aagTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGt > 1:118868/1‑102 (MQ=255) aagTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGt < 2:118868/102‑1 (MQ=255) tctcAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGgaga > 1:1017004/1‑118 (MQ=255) tctcAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGgaga < 2:1017004/118‑1 (MQ=255) ctcAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAatta > 3:19044/1‑131 (MQ=255) ctcAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAatta < 4:19044/131‑1 (MQ=255) cAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTatga > 2:440566/1‑68 (MQ=255) cAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTatga < 1:440566/68‑1 (MQ=255) cAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAAtca < 2:699253/104‑1 (MQ=255) cAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAAtca > 1:699253/1‑104 (MQ=255) aaCGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGtt < 4:491938/148‑1 (MQ=255) cGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCa < 3:877736/87‑1 (MQ=255) cGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCa > 4:877736/1‑87 (MQ=255) aTACAGAATTTCTAATCTGCATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCgg > 2:224959/1‑129 (MQ=255) aTACAGAATTTCTAATCTGCATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCgg < 1:224959/129‑1 (MQ=255) aCAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGt > 1:990539/1‑85 (MQ=255) aCAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGt < 2:990539/85‑1 (MQ=255) aCAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAattat > 1:519086/1‑119 (MQ=255) aCAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAattat < 2:519086/119‑1 (MQ=255) gAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCgg > 2:43463/1‑124 (MQ=255) gAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCgg < 1:43463/124‑1 (MQ=255) aaTTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTAt > 3:937598/1‑107 (MQ=255) aaTTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTAt < 4:937598/107‑1 (MQ=255) ttCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAAtcaggtcag > 4:237340/1‑92 (MQ=255) ttCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAAtcaggtcag < 3:237340/92‑1 (MQ=255) gATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTac < 4:501384/142‑1 (MQ=255) gATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTac > 3:501384/1‑142 (MQ=255) | CACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTAC > NC_000913/504705‑504963 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |