Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 513,464 C→A 5.5% L297M (CTG→ATG) ‡ ybaT → putative amino acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913513,4640CA 5.5% 460.8 / 13.3 127L297M (CTG→ATG) ‡ybaTputative amino acid transporter
Reads supporting (aligned to +/- strand):  ref base C (60/60);  new base A (3/4);  total (63/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01

Only 100 of 129 total aligned reads displayed.

TTGCTTAGCGATGTATCGGCATTAGAGTTAGAAAAATATGCCGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTA  >  NC_000913/513317‑513610
                                                                                                                                                   |                                                                                                                                                  
        cGATGTATCGGCATTAGAGTTAGAAAAATATGCCGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACAGGTTCGcg                                                                                                                                            <  2:329170/148‑2 (MQ=255)
                cGGCATTAGAGTTAGAAAAATATGCCGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTaa                                                                                                                                    >  1:852475/1‑148 (MQ=255)
                cGGCATTAGAGTTAGAAAAATATGCCGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTaa                                                                                                                                    >  1:842669/1‑148 (MQ=255)
                cGGCATTAGAGTTAGAAAAATATGCCGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTaa                                                                                                                                    >  3:816554/1‑148 (MQ=255)
                cGGCATTAGAGTTAGAAAAATATGCCGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACAGGTTCGcgt                                                                                                                                           >  1:1014560/1‑139 (MQ=255)
                cGGCATTAGAGTTAGAAAAATATGCCGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACAGGTTCGcgt                                                                                                                                           <  2:1014560/141‑3 (MQ=255)
                  gCATTAGAGTTAGAAAAATATGCCGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAAca                                                                                                                                  <  2:842669/148‑1 (MQ=255)
                   cATTAGAGTTAGAAAAATATGCCGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAAcat                                                                                                                                 >  1:476321/1‑148 (MQ=255)
                   cATTAGAGTTAGAAAAATATGCCGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAAcat                                                                                                                                 <  4:816554/148‑1 (MQ=255)
                     ttAGAGTTAGAAAAATATGCCGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGc                                                                                                                                             <  1:748610/134‑1 (MQ=255)
                     ttAGAGTTAGAAAAATATGCCGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGc                                                                                                                                             >  2:748610/1‑134 (MQ=255)
                     ttAGAGTTAGAAAAATATGCCGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAAcatca                                                                                                                               <  2:708718/148‑1 (MQ=255)
                      tAGAGTTAGAAAAATATGCCGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACAGGTTCGcgt                                                                                                                                           >  2:108323/1‑133 (MQ=255)
                        gagTTAGAAAAATATGCCGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATgg                                                                                                                            <  2:454256/148‑1 (MQ=255)
                         agTTAGAAAAATATGCCGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTa                                                                                                                                     <  2:1014415/138‑1 (MQ=255)
                         agTTAGAAAAATATGCCGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTa                                                                                                                                     >  1:1014415/1‑138 (MQ=255)
                         agTTAGAAAAATATGCCGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGa                                                                                                                           <  2:476321/148‑1 (MQ=255)
                            tAGAAAAATATGCCGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAAc                                                                                                                                   >  3:1051077/1‑137 (MQ=255)
                            tAGAAAAATATGCCGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAAc                                                                                                                                   <  4:1051077/137‑1 (MQ=255)
                            tAGAAAAATATGCCGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGacaa                                                                                                                        >  3:14453/1‑148 (MQ=255)
                                  aaTATGCCGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCgtg                                                                                                                                         <  1:160392/125‑1 (MQ=255)
                                  aaTATGCCGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCgtg                                                                                                                                         >  2:160392/1‑125 (MQ=255)
                                        ccGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAgcga                                                                                                            <  3:904190/148‑1 (MQ=255)
                                         cGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAAcatc                                                                                                                                >  2:666693/1‑127 (MQ=255)
                                         cGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAAcatc                                                                                                                                <  1:666693/127‑1 (MQ=255)
                                          gATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTaa                                                                                                                                    <  3:1085889/122‑1 (MQ=255)
                                          gATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAgcgaac                                                                                                          >  4:179010/1‑148 (MQ=255)
                                                gccgTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAgcgaacgcgaac                                                                                                    >  3:828982/1‑148 (MQ=255)
                                                 ccgTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACt                                                                                                   <  4:828982/148‑1 (MQ=255)
                                                  cgTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCgtg                                                                                                                                         >  3:226452/1‑109 (MQ=255)
                                                  cgTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCgtg                                                                                                                                         <  4:226452/109‑1 (MQ=255)
                                                       cgcAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGaa                                                                                             >  2:852129/1‑148 (MQ=255)
                                                         cAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGc                                                                                           <  3:179010/148‑1 (MQ=255)
                                                                ctTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGacaac                                                                                                                       <  4:750768/113‑1 (MQ=255)
                                                                ctTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGacaac                                                                                                                       >  3:750768/1‑113 (MQ=255)
                                                                ctTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGaa                                                                                    <  2:852475/148‑1 (MQ=255)
                                                                     ccGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAAt                                                                               >  3:453777/1‑148 (MQ=255)
                                                                          gctCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCtg                                                                          <  4:453777/148‑1 (MQ=255)
                                                                            tCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGa                                                                                                                           <  3:709059/97‑1 (MQ=255)
                                                                            tCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGa                                                                                                                           >  4:709059/1‑97 (MQ=255)
                                                                             cGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTaa                                                                                        >  3:622198/1‑131 (MQ=255)
                                                                             cGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTaa                                                                                        <  4:622198/131‑1 (MQ=255)
                                                                                   tgtgGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATaaa                                                                                >  1:380833/1‑133 (MQ=255)
                                                                                       ggTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGttt                                                                                                                                      <  1:985291/75‑1 (MQ=255)
                                                                                       ggTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGttt                                                                                                                                      >  2:985291/1‑75 (MQ=255)
                                                                                        gTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTAc                                                            >  1:853814/1‑148 (MQ=255)
                                                                                              tgATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAAcatcat                                                                                                                              >  1:746943/1‑76 (MQ=255)
                                                                                                aTCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATTAATCCCTGTGGCGGCag                                                                 >  1:731871/1‑135 (MQ=255)
                                                                                                aTCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCag                                                                 <  2:731871/135‑1 (MQ=255)
                                                                                                 tcgtcgTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTCCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTa                                                                                         >  2:1090661/1‑110 (MQ=255)
                                                                                                 tcgtcgTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTCCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTa                                                                                         <  1:1090661/110‑1 (MQ=255)
                                                                                                  cgtcgtCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATgg                                                                                                                   >  1:611329/1‑83 (MQ=255)
                                                                                                           cGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACAt                                                                                                                     <  2:500286/72‑1 (MQ=255)
                                                                                                           cGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACAt                                                                                                                     >  1:500286/1‑72 (MQ=255)
                                                                                                             gcgcTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATg                                                                                                                    <  4:179150/71‑1 (MQ=255)
                                                                                                             gcgcTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATg                                                                                                                    >  3:179150/1‑71 (MQ=255)
                                                                                                                 tttACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTa                                                                                         <  1:242283/94‑1 (MQ=255)
                                                                                                                  ttACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCagag                                                               >  3:393744/1‑119 (MQ=255)
                                                                                                                  ttACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCagag                                                               <  4:393744/119‑1 (MQ=255)
                                                                                                                  ttACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTa                                                             >  2:35558/1‑121 (MQ=255)
                                                                                                                  ttACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTa                                                             <  1:35558/121‑1 (MQ=255)
                                                                                                                  ttACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTggg                                                       >  1:477737/1‑127 (MQ=255)
                                                                                                                  ttACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGgtgt                                  >  2:767998/1‑148 (MQ=255)
                                                                                                                        gCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTa                            <  1:767998/148‑1 (MQ=255)
                                                                                                                           aCGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTg                                >  2:447159/1‑141 (MQ=255)
                                                                                                                           aCGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTg                                <  1:447159/141‑1 (MQ=255)
                                                                                                                              gCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATgg                                                                                                                            <  2:551495/46‑1 (MQ=255)
                                                                                                                              gCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAAt                                                                               <  4:601504/91‑1 (MQ=255)
                                                                                                                              gCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAAt                                                                               >  3:601504/1‑91 (MQ=255)
                                                                                                                              gCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCgtggtg                                   >  3:1039327/1‑135 (MQ=255)
                                                                                                                              gCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCgtggtg                                   <  4:1039327/135‑1 (MQ=255)
                                                                                                                                 tCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCgtgg                                     <  4:549017/130‑1 (MQ=255)
                                                                                                                                 tCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCgtgg                                     >  3:549017/1‑130 (MQ=255)
                                                                                                                                    gCCATTAACGCGAACCTGTTCGCCGTGTTTAAcat                                                                                                                                 <  4:723791/35‑1 (MQ=255)
                                                                                                                                    gCCATTAACGCGAACCTGTTCGCCGTGTTTAAcat                                                                                                                                 >  3:723791/1‑35 (MQ=255)
                                                                                                                                    gCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGAcgg                <  4:14453/148‑1 (MQ=255)
                                                                                                                                     ccATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTggcggc                                                                   <  1:1064533/96‑1 (MQ=255)
                                                                                                                                     ccATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTggcggc                                                                   >  2:1064533/1‑96 (MQ=255)
                                                                                                                                      cATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACat                                                >  4:1025356/1‑114 (MQ=255)
                                                                                                                                      cATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACat                                                <  3:1025356/114‑1 (MQ=255)
                                                                                                                                      cATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGAt                              <  1:382228/132‑1 (MQ=255)
                                                                                                                                      cATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGAcggcg              >  2:560197/1‑148 (MQ=255)
                                                                                                                                      cATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTAACTGGGGCAACATTATTGTCGAGGTGTTGAt                              >  2:382228/1‑132 (MQ=255)
                                                                                                                                         tAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTaa                                                                                        <  3:784482/71‑1 (MQ=255)
                                                                                                                                         tAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTaa                                                                                        >  4:784482/1‑71 (MQ=255)
                                                                                                                                           cggcgAACAGGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATaa                                                                                 >  2:706317/3‑76 (MQ=255)
                                                                                                                                           cggcgAACAGGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATaa                                                                                 <  1:706317/74‑1 (MQ=255)
                                                                                                                                           aCGCGAACCTGTTCGCGGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGaa                                                                                    <  2:916814/73‑1 (MQ=255)
                                                                                                                                           aCGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATg                                                                                      <  2:755284/71‑1 (MQ=255)
                                                                                                                                           aCGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGaa                                                                                    >  1:916814/1‑73 (MQ=255)
                                                                                                                                           aCGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTggcg                                                                     >  2:979829/1‑88 (MQ=255)
                                                                                                                                            cgcgAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCTGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTAt                           <  1:824653/129‑1 (MQ=255)
                                                                                                                                            cgcgAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCt                                                          >  1:1070230/1‑98 (MQ=255)
                                                                                                                                            cgcgAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGa                               <  4:2161/125‑1 (MQ=255)
                                                                                                                                            cgcgAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGa                               >  3:2161/1‑125 (MQ=255)
                                                                                                                                            cgcgAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTAt                           >  2:824653/1‑129 (MQ=255)
                                                                                                                                              cgAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGAcggcg              <  2:296005/140‑1 (MQ=255)
                                                                                                                                              cgAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACggcggc            >  4:1104558/1‑142 (MQ=255)
                                                                                                                                                aaCCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTg                                <  1:816250/120‑1 (MQ=255)
                                                                                                                                                  ccTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTa  <  1:560197/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTGCTTAGCGATGTATCGGCATTAGAGTTAGAAAAATATGCCGATACCGCCGTAGCGCAGGCTGCTTCTCCGCTGCTCGGGCATGTGGGTTATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTA  >  NC_000913/513317‑513610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: