Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 585,866 G→A 15.5% intergenic (‑233/‑191) ompT ← / → pauD DLP12 prophage; outer membrane protease VII; outer membrane protein 3b/tRNA‑OTHER

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913585,8660GA15.5% 165.9 / 18.6 64intergenic (‑233/‑191)ompT/pauDDLP12 prophage; outer membrane protease VII; outer membrane protein 3b/tRNA‑OTHER
Reads supporting (aligned to +/- strand):  ref base G (28/26);  new base A (5/5);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAGTATTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTG  >  NC_000913/585732‑586006
                                                                                                                                      |                                                                                                                                            
tAGTATTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTagag                                                                                                                                            >  1:871630/1‑137 (MQ=255)
tAGTATTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTagag                                                                                                                                            <  2:871630/137‑1 (MQ=255)
                 cacaATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCa                                                                                                                                         <  4:850182/123‑1 (MQ=255)
                 cacaATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCa                                                                                                                                         >  3:850182/1‑123 (MQ=255)
                                         ttAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGc                                                                                                                                          <  4:392444/98‑1 (MQ=255)
                                         ttAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGc                                                                                                                                          >  3:392444/1‑98 (MQ=255)
                                                     tATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATg                                                                                                                      <  4:843655/106‑1 (MQ=255)
                                                     tATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATg                                                                                                                      >  3:843655/1‑106 (MQ=255)
                                                           ttCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGa                                                                                                                     >  2:664961/1‑101 (MQ=255)
                                                           ttCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGa                                                                                                                     <  1:664961/101‑1 (MQ=255)
                                                                  ttCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGtttttt                                                                                          <  3:243098/121‑1 (MQ=255)
                                                                  ttCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGtttttt                                                                                          >  4:243098/1‑121 (MQ=255)
                                                                   tCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCa                                                                                                         <  1:152141/105‑1 (MQ=255)
                                                                   tCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCa                                                                                                         >  2:152141/1‑105 (MQ=255)
                                                                      gTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAaga                                                                             <  3:202119/130‑1 (MQ=255)
                                                                      gTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAaga                                                                             >  4:202119/1‑130 (MQ=255)
                                                                        tttACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTa                                                                                                     >  1:854462/1‑104 (MQ=255)
                                                                        tttACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTa                                                                                                     <  2:854462/104‑1 (MQ=255)
                                                                           aCTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTAt                                                                                                              <  2:991206/92‑1 (MQ=255)
                                                                           aCTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTAt                                                                                                              >  1:991206/1‑92 (MQ=255)
                                                                                   gTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGaaa                                                                                                                              <  2:988371/68‑1 (MQ=255)
                                                                                   gTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGaaa                                                                                                                              >  1:988371/1‑68 (MQ=255)
                                                                                    tttttttATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCata                                                                                                                                       >  3:7908/1‑58 (MQ=255)
                                                                                    tttttttATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCata                                                                                                                                       <  4:7908/58‑1 (MQ=255)
                                                                                                       tgCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATg                                                                                                                      >  1:39254/1‑56 (MQ=255)
                                                                                                       tgCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATg                                                                                                                      <  2:39254/56‑1 (MQ=255)
                                                                                                       tgCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGtttttt                                                                                          >  3:633226/1‑84 (MQ=255)
                                                                                                       tgCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGtttttt                                                                                          <  4:633226/84‑1 (MQ=255)
                                                                                                       tgCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAaga                                                                             >  2:390854/1‑97 (MQ=255)
                                                                                                       tgCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAaga                                                                             <  1:390854/97‑1 (MQ=255)
                                                                                                               tCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTg                                                                 >  2:234692/1‑101 (MQ=255)
                                                                                                               tCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTg                                                                 <  1:234692/101‑1 (MQ=255)
                                                                                                                  aTTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGa                                                                         >  4:401296/1‑90 (MQ=255)
                                                                                                                  aTTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGa                                                                         <  3:401296/90‑1 (MQ=255)
                                                                                                                    ttAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAagag                                                                            <  4:1086114/85‑1 (MQ=255)
                                                                                                                    ttAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAagag                                                                            >  3:1086114/1‑85 (MQ=255)
                                                                                                                       aGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCaa                                             >  3:93505/1‑113 (MQ=255)
                                                                                                                       aGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCaa                                             <  4:93505/113‑1 (MQ=255)
                                                                                                                          tataTGCCTCTAAAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTa                                                                                         >  2:263492/4‑66 (MQ=39)
                                                                                                                          tataTGCCTCTAAAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTa                                                                                         <  1:263492/63‑1 (MQ=39)
                                                                                                                          tataTGCCTCTAAAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACaaaa                                                                    <  2:445266/84‑1 (MQ=255)
                                                                                                                          tataTGCCTCTAAAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACaaaa                                                                    >  1:445266/4‑87 (MQ=255)
                                                                                                                           ataTGCCTCTAAAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGttt                     <  1:106537/131‑1 (MQ=255)
                                                                                                                           ataTGCCTCTAAAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGttt                     >  2:106537/3‑133 (MQ=255)
                                                                                                                            taTGCCTCTAAAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTa                                                                                                     <  2:775282/51‑1 (MQ=38)
                                                                                                                            taTGCCTCTAAAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTa                                                                                                     >  1:775282/2‑52 (MQ=38)
                                                                                                                            taTGCCTCTAAAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGtttttt                                                                                          >  2:684060/2‑63 (MQ=255)
                                                                                                                            taTGCCTCTAAAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGtttttt                                                                                          <  1:684060/62‑1 (MQ=255)
                                                                                                                            cATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAACGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACgt     >  1:522464/1‑148 (MQ=255)
                                                                                                                             aTGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAg             <  3:815712/139‑1 (MQ=255)
                                                                                                                             aTGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAg             >  4:815712/1‑139 (MQ=255)
                                                                                                                              tGCCTTTAGAGGCATATTAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAag                                                                              <  1:283440/73‑1 (MQ=255)
                                                                                                                              tGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCTTCAATTACTATGTTTTTTAGTACAAAAAag                                                                              >  2:283440/1‑73 (MQ=255)
                                                                                                                              tGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATg                        >  2:1036958/1‑127 (MQ=255)
                                                                                                                              tGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATg                        <  1:1036958/127‑1 (MQ=255)
                                                                                                                              tGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGa                  <  3:970047/133‑1 (MQ=255)
                                                                                                                              tGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGa                  >  4:970047/1‑133 (MQ=255)
                                                                                                                              tGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACgtgt   >  1:740600/1‑148 (MQ=255)
                                                                                                                                ccTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGtttttt                                                                                          <  4:546864/59‑1 (MQ=255)
                                                                                                                                ccTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGtttttt                                                                                          >  3:546864/1‑59 (MQ=255)
                                                                                                                                  tttAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTg                                                                 >  3:984288/1‑82 (MQ=255)
                                                                                                                                  tttAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTg                                                                 <  4:984288/82‑1 (MQ=255)
                                                                                                                                   ttAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCaa                                             >  3:934520/1‑101 (MQ=255)
                                                                                                                                   ttAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCaa                                             <  4:934520/101‑1 (MQ=255)
                                                                                                                                   ttAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGtgtg  >  2:248135/1‑144 (MQ=255)
                                                                                                                                   ttAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGtgtg  <  1:248135/144‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                            
TAGTATTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTG  >  NC_000913/585732‑586006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: