Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 595,519 T→A 8.3% intergenic (‑76/‑81) cusR ← / → cusC response regulator in two‑component regulatory system with CusS/copper/silver efflux system, outer membrane component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913595,5190TA 8.3% 161.7 / 5.7 48intergenic (‑76/‑81)cusR/cusCresponse regulator in two‑component regulatory system with CusS/copper/silver efflux system, outer membrane component
Reads supporting (aligned to +/- strand):  ref base T (22/22);  new base A (2/2);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

TCGGTTAACCCTTTGGTCAAGTATTCTCCGGTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGT  >  NC_000913/595379‑595653
                                                                                                                                            |                                                                                                                                      
tCGGTTAACCCTTTGGTCAAGTATTCTCCGGTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCa                                                                                                                                 >  3:682922/1‑148 (MQ=255)
      aaCCCTTTGGTCAAGTATTCTCCGGTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTc                                                                                                                           <  4:682922/148‑1 (MQ=255)
                   aatATTCTCCGGTTTTCTTTTCATCTTCGACAAGCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGaaaa                                                                                                              >  3:756728/3‑148 (MQ=255)
                    gTATTCTCCGGTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAAt                                                                                                             <  4:756728/148‑1 (MQ=255)
                           ccGGTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGcc                                                                                                      >  1:380636/1‑148 (MQ=255)
                                             tCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAAt                                                                                                             >  1:1124909/1‑123 (MQ=255)
                                             tCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAAt                                                                                                             <  2:1124909/123‑1 (MQ=255)
                                                       aCAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTgg                                                                                      <  2:670467/136‑1 (MQ=255)
                                                       aCAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTgg                                                                                      >  1:670467/1‑136 (MQ=255)
                                                          gTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAAttt                                                                                                                                      <  3:843781/85‑1 (MQ=255)
                                                          gTTTCATATTTCCTACGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAAttt                                                                                                                                      >  4:843781/1‑85 (MQ=255)
                                                                tatTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTgg                                                                                                   >  1:698405/1‑114 (MQ=255)
                                                                tatTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTgg                                                                                                   <  2:698405/114‑1 (MQ=255)
                                                                   ttCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCAtt                                                                                                                               <  2:207473/83‑1 (MQ=255)
                                                                   ttCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCAtt                                                                                                                               >  1:207473/1‑83 (MQ=255)
                                                                   ttCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTg                                                                                       >  3:840135/1‑123 (MQ=255)
                                                                   ttCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTg                                                                                       <  4:840135/123‑1 (MQ=255)
                                                                   ttCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAAAATTGTCAtt                                                                                                                               >  2:994598/1‑83 (MQ=255)
                                                                   ttCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGCCAAAATGACAAAATTGTCAtt                                                                                                                               <  1:994598/83‑1 (MQ=255)
                                                                     cctccGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTc                                                                                                                           <  2:346964/85‑1 (MQ=255)
                                                                     cctccGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTc                                                                                                                           >  1:346964/1‑85 (MQ=255)
                                                                           cATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCa                                                                         <  4:301272/129‑1 (MQ=255)
                                                                           cATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCa                                                                         >  3:301272/1‑129 (MQ=255)
                                                                             tGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTATCACCGGAAAATCAGAGCCTGGCGAGTAAAGtt                                                                                        <  4:1105514/112‑1 (MQ=255)
                                                                             tGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTATCACCGGAAAATCAGAGCCTGGCGAGTAAAGtt                                                                                        >  3:1105514/1‑112 (MQ=255)
                                                                                tGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGtctc                                                 <  2:380636/148‑1 (MQ=255)
                                                                                    cGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTg                                             >  3:1132507/1‑148 (MQ=255)
                                                                                       gCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTaa                                          <  4:1132507/148‑1 (MQ=255)
                                                                                               agagTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATc                                                                          >  3:118267/1‑108 (MQ=255)
                                                                                               agagTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATc                                                                          >  3:163089/1‑108 (MQ=255)
                                                                                               agagTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATc                                                                          <  4:118267/108‑1 (MQ=255)
                                                                                               agagTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATc                                                                          <  4:163089/108‑1 (MQ=255)
                                                                                                          aTCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCt                                               >  2:1025062/1‑124 (MQ=255)
                                                                                                          aTCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCt                                               <  1:1025062/124‑1 (MQ=255)
                                                                                                               aTGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTgg                                                                                      >  3:920542/1‑80 (MQ=255)
                                                                                                               aTGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTgg                                                                                      <  4:920542/80‑1 (MQ=255)
                                                                                                                      tCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACtt                                      <  3:901186/121‑1 (MQ=255)
                                                                                                                      tCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACtt                                      >  4:901186/1‑121 (MQ=255)
                                                                                                                           tATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATg                                                             <  3:186671/93‑1 (MQ=255)
                                                                                                                           tATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATg                                                             >  4:186671/1‑93 (MQ=255)
                                                                                                                                 cAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCg                                                                                                 <  2:1085724/51‑1 (MQ=255)
                                                                                                                                 cAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCg                                                                                                 >  1:1085724/1‑51 (MQ=255)
                                                                                                                                  aaaaTGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCAcc                                                                       <  3:490660/76‑1 (MQ=255)
                                                                                                                                  aaaaTGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCAcc                                                                       >  4:490660/1‑76 (MQ=255)
                                                                                                                                  aaaaTGACAAAATTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTc                                     <  2:378941/110‑1 (MQ=255)
                                                                                                                                  aaaaTGACAAAATTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTc                                     >  1:378941/1‑110 (MQ=255)
                                                                                                                                           aTTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTg                                   <  2:75550/103‑1 (MQ=255)
                                                                                                                                           aTTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTg                                   >  1:75550/1‑103 (MQ=255)
                                                                                                                                           aTTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGgttgt  >  2:516350/1‑136 (MQ=255)
                                                                                                                                           aTTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGgttgt  <  1:516350/136‑1 (MQ=255)
                                                                                                                                            ttttGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTc                                     >  3:632418/1‑100 (MQ=255)
                                                                                                                                            ttttGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTc                                     <  4:632418/100‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                      
TCGGTTAACCCTTTGGTCAAGTATTCTCCGGTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGT  >  NC_000913/595379‑595653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: