Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 623,062 A→T 8.6% M255L (ATG→TTG)  entS → enterobactin exporter, iron‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913623,0620AT 8.6% 234.9 / 10.8 70M255L (ATG→TTG) entSenterobactin exporter, iron‑regulated
Reads supporting (aligned to +/- strand):  ref base A (33/31);  new base T (2/4);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

AGCATCCGTTGAAATCATTACTGGCAGGATTTCGTTTTCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTGGCACATAGTGCGCGACCAGGGTTATTGATGCTGCTCTCCACGCTGGGATCGTTCCTCGCCA  >  NC_000913/622919‑623203
                                                                                                                                               |                                                                                                                                             
aGCATCCGTTGAAATCATTACTGGCAGGATTTCGTTTTCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACATCTGCCAGTTGTc                                                                                                                                           <  4:689717/148‑1 (MQ=255)
aGCATCCGTTGAAATCATTACTGGCAGGATTTCGTTTTCTGCATGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACCTCTGCCAGTTGTc                                                                                                                                           <  3:77637/148‑1 (MQ=255)
            aaTCATTACTGGCAGGATTTCGTTTTCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGAt                                                                                                                               >  2:435827/1‑148 (MQ=255)
              tCATTACTGGCAGGATTTCGTTTTCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGt                                                                                                                                            >  2:613755/1‑133 (MQ=255)
              tCATTACTGGCAGGATTTCGTTTTCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGt                                                                                                                                            <  1:613755/133‑1 (MQ=255)
                        cAGGATTTCGTTTTCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTa                                                                                                                   <  2:364882/148‑1 (MQ=255)
                              ttCGTTTTCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTtct                                                                                                                      >  1:1009868/1‑139 (MQ=255)
                              ttCGTTTTCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTtct                                                                                                                      >  3:677595/1‑139 (MQ=255)
                              ttCGTTTTCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTtct                                                                                                                      <  4:677595/139‑1 (MQ=255)
                              ttCGTTTTCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTtct                                                                                                                      <  2:1009868/139‑1 (MQ=255)
                                   tttCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGt                                                                                                                           <  2:567896/129‑1 (MQ=255)
                                   tttCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGt                                                                                                                           >  1:567896/1‑129 (MQ=255)
                                       tGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCt                                                                                                    <  1:435827/148‑1 (MQ=255)
                                                     cTGGTGGGCGGGATTGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTg                                                                                      >  4:818752/1‑148 (MQ=255)
                                                             cGGGATTGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGtttt                                                                                                                        >  3:158668/1‑106 (MQ=255)
                                                             cGGGATTGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGtttt                                                                                                                        <  4:158668/106‑1 (MQ=255)
                                                               ggATTGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAAc                                                                            >  1:806673/1‑148 (MQ=255)
                                                                  ttGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACgcggcg                                                                                                            >  4:173755/1‑113 (MQ=255)
                                                                  ttGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACgcggcg                                                                                                            <  3:173755/113‑1 (MQ=255)
                                                                  ttGCGCTGCTGGGTGGTTTATGGACGATGGCGAGCGCGGTGCGGGTACTGAATCCGGCGCTGGCTGACAAGTGGCAGATGTCAGCGGCACAGATTGGTTTGCTCTACGAGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAGCCAg                                                                         >  4:710207/1‑148 (MQ=255)
                                                                       ctgctgGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTgg                                                                                                                            <  4:756125/92‑1 (MQ=255)
                                                                       cagctgGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTgg                                                                                                                            >  3:756125/3‑92 (MQ=255)
                                                                            gggTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTg                                                               <  3:818752/148‑1 (MQ=255)
                                                                              gtggtTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTGGc                                                             >  3:75748/1‑148 (MQ=255)
                                                                                  tttATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAAcc                                                                           <  4:593104/130‑1 (MQ=255)
                                                                                  tttATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAAcc                                                                           >  3:593104/1‑130 (MQ=255)
                                                                                   ttATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACgcgg                                                                                                              <  4:49442/94‑1 (MQ=255)
                                                                                   ttATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACgcgg                                                                                                              >  3:49442/1‑94 (MQ=255)
                                                                                     aTTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTGGCACATAGt                                                      >  1:282640/1‑148 (MQ=255)
                                                                                       tGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGa                                                                    >  4:477353/1‑132 (MQ=255)
                                                                                       tGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGa                                                                    <  3:477353/132‑1 (MQ=255)
                                                                                        gACGATGGGGAGCGCGGTGCGGGTACTGTATCAGGCGCTGACTGACAACTGGCAGATGTCATCGGCACAGATTGGTTTTCTCTACGCGGCAATCCCGCTCGGCGCGGCTATTCGTGCGTTAACCAGCGGGAAGCTGGCACATAGTgcg                                                   <  3:710207/148‑1 (MQ=255)
                                                                                          cGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACATCTGCCAGTTGTCAg                                                                                                                                         <  4:265555/60‑1 (MQ=37)
                                                                                          cGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACATCTGCCAGTTGTCAg                                                                                                                                         >  3:265555/1‑60 (MQ=37)
                                                                                          cGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGttt                                                                                                                         >  3:566250/1‑76 (MQ=255)
                                                                                          cGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGttt                                                                                                                         <  4:566250/76‑1 (MQ=255)
                                                                                              ggCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTGGCACATAGTGCGCGACCa                                             <  2:806673/148‑1 (MQ=255)
                                                                                               gCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGTCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTGGCACATAGTGCGCGACCAg                                            <  4:75748/148‑1 (MQ=255)
                                                                                                  aGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGcgcg                                                                                             >  4:757423/1‑96 (MQ=255)
                                                                                                  aGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGcgcg                                                                                             <  3:757423/96‑1 (MQ=255)
                                                                                                         tGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGgcg                                                                                               <  1:543398/87‑1 (MQ=255)
                                                                                                         tGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGgcg                                                                                               >  2:543398/1‑87 (MQ=255)
                                                                                                               tACTGTATCCGGCGCTGACTGACAACTGGCAGATGTCAGCGGCACCGATTGGt                                                                                                                           >  2:954856/1‑53 (MQ=255)
                                                                                                               tACTGTATCCGGCGCTGACTGACAACTGGCAGATGTCAGCGGCACAGATTGGt                                                                                                                           <  1:954856/53‑1 (MQ=255)
                                                                                                                   gTATCTGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTGGCACATAGTGCGCGACCAGGGTTATTGATGCTGCTCTcc                        >  3:723653/1‑148 (MQ=255)
                                                                                                                     aTCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTGGCACAt                                                         >  4:178007/1‑113 (MQ=255)
                                                                                                                     aTCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTGGCACAt                                                         >  1:659456/1‑113 (MQ=255)
                                                                                                                     aTCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTGGCACAt                                                         <  2:659456/113‑1 (MQ=255)
                                                                                                                     aTCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTGGCACAt                                                         <  3:178007/113‑1 (MQ=255)
                                                                                                                     aTCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTGGCACATAGt                                                      >  4:1074491/1‑116 (MQ=255)
                                                                                                                     aTCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTGGCACATAGt                                                      <  3:1074491/116‑1 (MQ=255)
                                                                                                                        cGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTGGCACATAGTGCGCGACCAGGGTTATTGATGCTGCTCTCCACGCt                   <  2:282640/148‑1 (MQ=255)
                                                                                                                             ctggctgACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTGGCACATAGTGCGCGACCAGGGTTATTGATGCTGCTCTCCACGCTGGGAt              >  3:1010670/1‑148 (MQ=255)
                                                                                                                             ctggctgACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTGGCACATAGTGCGCGACCAGGGTTATTGATGCTGCTCTCCACGCTGGGAt              >  3:967237/1‑148 (MQ=255)
                                                                                                                                  tgACATCTGCCAGTTGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTGGCACATAGTGCGCGACCAGGGTTATTGATGCTGCTCTCCACGCTGGGATCGTTc         >  1:44354/1‑148 (MQ=255)
                                                                                                                                    aCATCTGCCAGTTGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTGGCACATAGTGCGCGACCAGGGTTATTGATGCTGCTCTCCACGCTGGGATCGTTCCt       <  2:44354/148‑1 (MQ=255)
                                                                                                                                    aCAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGaa                                                                   <  4:620141/88‑1 (MQ=255)
                                                                                                                                    aCAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGaa                                                                   >  3:620141/1‑88 (MQ=255)
                                                                                                                                     cAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTgg                                                                                     <  3:529987/69‑1 (MQ=255)
                                                                                                                                     cAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTgg                                                                                     >  4:529987/1‑69 (MQ=255)
                                                                                                                                     cAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTGGCACATAGTGCGCGACCAGGGTTATTGATGCTGCTCTCCACGCTGGGATCGTTCCTc      >  1:138261/1‑148 (MQ=255)
                                                                                                                                       aCTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTGGCACATAGTGCGCGACCAGGGt                                         <  4:367153/111‑1 (MQ=255)
                                                                                                                                       aCTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTGGCACATAGTGCGCGACCAGGGt                                         >  3:367153/1‑111 (MQ=255)
                                                                                                                                       aCTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTGGCACATAGTGCGCGACCAGGGTTATTGATGCTGCTCTCCACGCTGGGATCGtt          >  2:480622/1‑142 (MQ=255)
                                                                                                                                       aCTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTGGCACATAGTGCGCGACCAGGGTTATTGATGCTGCTCTCCACGCTGGGATCGtt          <  1:480622/142‑1 (MQ=255)
                                                                                                                                         tGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTGGCACATAGTGCGCGACCAGGGTTATTGATGCTGCTTTCCACGCTGGGATCGTTCCTCGCCa  <  4:723653/148‑1 (MQ=255)
                                                                                                                                           gCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTGGCACATAGTGCGCGACCAGGGTTATTGAtgctgc                             <  2:1028268/119‑1 (MQ=255)
                                                                                                                                           gCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTGGCACATAGTGCGCGACCAGGGTTATTGAtgctgc                             >  1:1028268/1‑119 (MQ=255)
                                                                                                                                            cAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAAcc                                                                           >  2:397534/1‑72 (MQ=255)
                                                                                                                                            cAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAAcc                                                                           <  1:397534/72‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                             
AGCATCCGTTGAAATCATTACTGGCAGGATTTCGTTTTCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCAGATGTCAGCGGCACAGATTGGTTTTCTCTACGCGGCGATCCCGCTCGGCGCGGCTATTGGTGCGTTAACCAGCGGGAAGCTGGCACATAGTGCGCGACCAGGGTTATTGATGCTGCTCTCCACGCTGGGATCGTTCCTCGCCA  >  NC_000913/622919‑623203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: