Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 715,756 G→T 7.7% Q115H (CAG→CAT) ‡ ybfP → lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913715,7560GT 7.7% 219.6 / 5.8 63Q115H (CAG→CAT) ‡ybfPlipoprotein
Reads supporting (aligned to +/- strand):  ref base G (29/29);  new base T (2/3);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACAT  >  NC_000913/715617‑715903
                                                                                                                                           |                                                                                                                                                   
cTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGCTTGCCCTGGCAAAcc                                                                                                                                             <  3:692512/148‑1 (MQ=255)
                            aGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATg                                                                                                                      >  2:391826/1‑143 (MQ=255)
                            aGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATg                                                                                                                      <  1:391826/143‑1 (MQ=255)
                                                               atatGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCga                                                                                  >  1:1057249/1‑144 (MQ=255)
                                                               atatGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCga                                                                                  <  2:1057249/144‑1 (MQ=255)
                                                                 atGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCgatga                                                                               >  4:661894/1‑145 (MQ=255)
                                                                 atGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCgatga                                                                               <  3:661894/145‑1 (MQ=255)
                                                                      aGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCgg                                                                                                >  4:831991/1‑123 (MQ=255)
                                                                      aGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGACAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCgg                                                                                                <  3:831991/123‑1 (MQ=255)
                                                                          caAGAAAAGGTCGGTTTGAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGa                                                                   >  4:456529/1‑148 (MQ=255)
                                                                          caAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCga                                                                                  <  1:550132/133‑1 (MQ=255)
                                                                          caAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCga                                                                                  >  2:550132/1‑133 (MQ=255)
                                                                           aaGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCaa                                                                                                                        <  3:849284/94‑1 (MQ=255)
                                                                           aaGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCaa                                                                                                                        >  4:849284/1‑94 (MQ=255)
                                                                            aGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAg                                                                                                                                               >  4:203671/1‑70 (MQ=255)
                                                                            aGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAg                                                                                                                                               <  3:203671/70‑1 (MQ=255)
                                                                             gAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGAtttttt                                                                                         <  1:770733/123‑1 (MQ=255)
                                                                             gAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGAtttttt                                                                                         >  2:770733/1‑123 (MQ=255)
                                                                             gAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCga                                                                                  <  3:931517/130‑1 (MQ=255)
                                                                             gAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCga                                                                                  >  4:931517/1‑130 (MQ=255)
                                                                              aaaaGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGa                                                                                                                                   <  2:888126/80‑1 (MQ=255)
                                                                              aaaaGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGa                                                                                                                                   >  1:888126/1‑80 (MQ=255)
                                                                              aaaaGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAg                                                                                                                    >  4:423296/1‑95 (MQ=255)
                                                                              aaaaGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAg                                                                                                                    <  3:423296/95‑1 (MQ=255)
                                                                                aaGGTCGGTTTGAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTg                                                             <  3:456529/148‑1 (MQ=255)
                                                                                aaGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTa                                                                                                               >  2:376280/1‑98 (MQ=255)
                                                                                aaGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTa                                                                                                               >  2:280555/1‑98 (MQ=255)
                                                                                aaGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTa                                                                                                               <  1:280555/98‑1 (MQ=255)
                                                                                aaGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTa                                                                                                               <  1:376280/98‑1 (MQ=255)
                                                                                    tCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCa                                                                                                                         <  2:337090/84‑1 (MQ=255)
                                                                                    tCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCa                                                                                                                         >  1:337090/1‑84 (MQ=255)
                                                                                    tCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGa                                                                                                                     <  1:1015230/88‑1 (MQ=255)
                                                                                    tCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGa                                                                                                                     >  2:1015230/1‑88 (MQ=255)
                                                                                    tCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAg                                                                                                   >  4:1129462/1‑106 (MQ=255)
                                                                                    tCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAg                                                                                                   <  3:1129462/106‑1 (MQ=255)
                                                                                        tttAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCAATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTacga                                                     >  3:487477/1‑148 (MQ=255)
                                                                                         ttAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGc                                                                                                                                              <  4:521893/58‑1 (MQ=255)
                                                                                         ttAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGc                                                                                                                                              >  3:521893/1‑58 (MQ=255)
                                                                                         ttAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGatgatg                                                                             <  3:274439/123‑1 (MQ=255)
                                                                                         ttAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGatgatg                                                                             >  4:274439/1‑123 (MQ=255)
                                                                                           aaaTCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCAATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGc                                                  <  4:487477/148‑1 (MQ=255)
                                                                                               cccGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAgg                                                                                                                                                    <  2:261967/46‑1 (MQ=255)
                                                                                               cccGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAgg                                                                                                                                                    >  1:261967/1‑46 (MQ=255)
                                                                                               cccGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTa                                                        <  3:175238/138‑1 (MQ=255)
                                                                                               cccGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTa                                                        >  4:175238/1‑138 (MQ=255)
                                                                                                    ttGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAg                                                                                                                    <  2:250777/73‑1 (MQ=255)
                                                                                                    atGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAg                                                                                                                    >  1:250777/2‑73 (MQ=255)
                                                                                                        cAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAg                                                                                                         >  4:927455/1‑80 (MQ=255)
                                                                                                        cAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAg                                                                                                         <  3:927455/80‑1 (MQ=255)
                                                                                                               acacCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCAc                                                                                                                                       >  2:371278/1‑43 (MQ=255)
                                                                                                               acacCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCAc                                                                                                                                       <  1:371278/43‑1 (MQ=255)
                                                                                                                     cATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGc                                                                            <  3:967389/96‑1 (MQ=255)
                                                                                                                     cATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGcc                                              <  2:769930/126‑1 (MQ=255)
                                                                                                                     cATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGcc                                              <  4:631670/126‑1 (MQ=255)
                                                                                                                     cATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGcc                                              >  1:769930/1‑126 (MQ=255)
                                                                                                                     cATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGcc                                              >  3:631670/1‑126 (MQ=255)
                                                                                                                     cATTTTTTATGCGGGTTTACCAGGGCAAGCCCGGCACTCGACCGATAGTCACTGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGatgatga                                                                            >  4:967389/1‑95 (MQ=255)
                                                                                                                                  ggcttGCCCTGGCAAACCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTg                                                             >  2:475077/4‑98 (MQ=255)
                                                                                                                                  ggcttGCCCTGGCAAACCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTg                                                             <  1:475077/95‑1 (MQ=255)
                                                                                                                                  ggcttGCCCTGGCAAACCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAg                                        <  4:372251/116‑1 (MQ=255)
                                                                                                                                  ggcttGCCCTGGCAAACCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAg                                        >  3:372251/4‑119 (MQ=255)
                                                                                                                                     ttGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAAt                                   <  4:530653/121‑1 (MQ=255)
                                                                                                                                     ttGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAAt                                   >  3:530653/1‑121 (MQ=255)
                                                                                                                                          aGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGaa                  <  3:69410/133‑1 (MQ=255)
                                                                                                                                          aGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGaa                  >  4:69410/1‑133 (MQ=255)
                                                                                                                                           gggCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACAt  >  2:1038993/1‑148 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
CTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACAT  >  NC_000913/715617‑715903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑