Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 765,132 | G→A | 23.8% | intergenic (+83/+21) | sucD → / ← mngR | succinyl‑CoA synthetase, NAD(P)‑binding, alpha subunit/transcriptional repressor for the mannosyl‑D‑glycerate catabolic operon |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 765,132 | 0 | G | A | 23.8% | 34.1 / 10.3 | 21 | intergenic (+83/+21) | sucD/mngR | succinyl‑CoA synthetase, NAD(P)‑binding, alpha subunit/transcriptional repressor for the mannosyl‑D‑glycerate catabolic operon |
Reads supporting (aligned to +/- strand): ref base G (8/8); new base A (3/2); total (11/10) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.43e-01 |
TGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAA > NC_000913/765042‑765274 | tGAAATAAATCTCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGTAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTatgat > 3:374053/1‑97 (MQ=255) tGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGTCTGTCGGTTTCGACATGGTTGGCCATCGTatgat < 4:374053/97‑1 (MQ=255) taaataTCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTatga > 4:1059151/1‑91 (MQ=255) taaataTCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTatga < 3:1059151/91‑1 (MQ=255) aaGAAATAGCCCTCGCCGCTTCCCTCTACAGGAGTGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCATACGATGGc > 1:145405/1‑81 (MQ=255) aaGAAATAGCCCTCGCCGCTTCCCTCTACAGGAGTGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCATACGATGGc < 2:145405/81‑1 (MQ=255) aaGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTa > 3:855440/1‑74 (MQ=255) aaGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTa < 4:855440/74‑1 (MQ=255) ttCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTtgtg > 3:88230/1‑72 (MQ=255) ttCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTtgtg < 4:88230/72‑1 (MQ=255) gTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGAc > 3:131546/1‑132 (MQ=255) gTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGAc < 4:131546/132‑1 (MQ=255) ggTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTtat < 2:586759/84‑1 (MQ=255) ggTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTtat > 1:586759/1‑84 (MQ=255) tttCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTgcgc < 3:10902/65‑1 (MQ=255) tttCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTgcgc > 4:10902/1‑65 (MQ=255) ccATCATACGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGc < 2:113980/94‑1 (MQ=255) ccATCATACGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGc > 1:113980/1‑94 (MQ=255) ccATCATACGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGaa > 1:908163/1‑148 (MQ=255) cATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCAcg < 3:703307/130‑1 (MQ=255) cATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCAcg > 4:703307/1‑130 (MQ=255) | TGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAA > NC_000913/765042‑765274 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |