Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 828,746 T→C 5.6% H79R (CAC→CGC)  ybhF ← putative ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913828,7460TC 5.6% 248.4 / 3.3 71H79R (CAC→CGC) ybhFputative ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (35/32);  new base C (2/2);  total (37/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGA  >  NC_000913/828615‑828892
                                                                                                                                   |                                                                                                                                                  
gcagGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGGGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGtt                                                                                                                                    >  2:965729/1‑148 (MQ=255)
          aaaaGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCTAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGa                                                                                                                          <  1:965729/148‑1 (MQ=255)
           aaaGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGCGCTGCACGCcg                                                                                                                                         >  2:885471/1‑132 (MQ=255)
           aaaGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGCGCTGCACGCcg                                                                                                                                         <  1:885471/132‑1 (MQ=255)
              gTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCaa                                                                                                                      <  2:797289/148‑1 (MQ=255)
                         tGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTg                                                                                                                                <  1:1028300/127‑1 (MQ=255)
                         tGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTg                                                                                                                                >  2:1028300/1‑127 (MQ=255)
                                         cTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCg                                                                                                                            >  2:673928/1‑115 (MQ=255)
                                         cTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCg                                                                                                                            <  1:673928/115‑1 (MQ=255)
                                                        tACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGc                                                                                                 >  2:906258/1‑127 (MQ=255)
                                                        tACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGc                                                                                                 <  1:906258/127‑1 (MQ=255)
                                                         aCAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCaa                                                                                                                      <  2:1055026/105‑1 (MQ=255)
                                                         aCAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCaa                                                                                                                      >  1:1055026/1‑105 (MQ=255)
                                                         aCAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTc                                                                                          <  2:1034946/133‑1 (MQ=255)
                                                         aCAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTc                                                                                          >  1:1034946/1‑133 (MQ=255)
                                                         aCAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTc                                                                                   >  4:447376/1‑140 (MQ=255)
                                                         aCAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTc                                                                                   <  3:447376/140‑1 (MQ=255)
                                                              ttGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGAt                                                                                                                              <  3:913042/92‑1 (MQ=255)
                                                              ttGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGAt                                                                                                                              >  4:913042/1‑92 (MQ=255)
                                                              ttGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCa                                                                      >  2:578912/1‑148 (MQ=255)
                                                               tGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCaa                                                                                                                      >  1:337883/1‑99 (MQ=255)
                                                               tGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCaa                                                                                                                      <  2:337883/99‑1 (MQ=255)
                                                                    ttCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATcc                                                                >  2:475435/1‑148 (MQ=255)
                                                                    ttCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATcc                                                                >  1:251441/1‑148 (MQ=255)
                                                                      ctcCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGc                                                              >  4:424629/1‑148 (MQ=255)
                                                                         cATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATc                                                           <  1:578912/148‑1 (MQ=255)
                                                                          aTCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGt                                                                                                                                                    >  4:886312/1‑58 (MQ=255)
                                                                          aTCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGt                                                                                                                                                    <  3:886312/58‑1 (MQ=255)
                                                                          aTCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCcgtcg                                                                                                                                      >  4:322580/1‑72 (MQ=255)
                                                                          aTCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCcgtcg                                                                                                                                      <  3:322580/72‑1 (MQ=255)
                                                                          aTCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGtt                                                                                                                                    <  1:346519/74‑1 (MQ=255)
                                                                          aTCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGtt                                                                                                                                    >  2:346519/1‑74 (MQ=255)
                                                                          aTCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCgg                                                                                                                           >  1:606510/1‑83 (MQ=255)
                                                                          aTCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCgg                                                                                                                           <  2:606510/83‑1 (MQ=255)
                                                                                gTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTgg                                                    <  1:475435/148‑1 (MQ=255)
                                                                                    gaTCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGtttt                                                <  2:251441/148‑1 (MQ=255)
                                                                                       cTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTAcc                                             <  3:424629/148‑1 (MQ=255)
                                                                                         tCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGtt                                                                                     >  3:850177/1‑106 (MQ=255)
                                                                                         tCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGtt                                                                                     <  4:850177/106‑1 (MQ=255)
                                                                                             aCAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGAt                                                                                                                              <  4:298893/61‑1 (MQ=255)
                                                                                             aCAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGAt                                                                                                                              >  3:298893/1‑61 (MQ=255)
                                                                                             aCAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCAcc                                       >  2:298903/1‑148 (MQ=255)
                                                                                                   cAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAAt                                                                                                               >  1:110906/1‑70 (MQ=255)
                                                                                                   cAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAAt                                                                                                               <  2:110906/70‑1 (MQ=255)
                                                                                                    aaaTTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTc                                                                                   <  1:155097/97‑1 (MQ=255)
                                                                                                    aaaTTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTc                                                                                   >  2:155097/1‑97 (MQ=255)
                                                                                                     aaTTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCa                                                                                                                 >  1:22135/1‑66 (MQ=255)
                                                                                                     aaTTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCa                                                                                                                 <  2:22135/66‑1 (MQ=255)
                                                                                                                 atatAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCaa                                                                     <  1:390869/98‑1 (MQ=255)
                                                                                                                 atatAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCaa                                                                     >  2:390869/1‑98 (MQ=255)
                                                                                                                   ataACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATc                                                           >  3:136782/1‑106 (MQ=255)
                                                                                                                   ataACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATc                                                           <  4:136782/106‑1 (MQ=255)
                                                                                                                    taACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTaa                                                                              <  2:888555/86‑1 (MQ=255)
                                                                                                                    taACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTaa                                                                              >  1:888555/1‑86 (MQ=255)
                                                                                                                    taACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTaa                                                                              >  3:178346/1‑86 (MQ=255)
                                                                                                                    taACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTaa                                                                              <  4:178346/86‑1 (MQ=255)
                                                                                                                    taACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGc                                <  2:774632/132‑1 (MQ=255)
                                                                                                                    taACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGc                                >  1:774632/1‑132 (MQ=255)
                                                                                                                      aCCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTa                                               <  3:387452/115‑1 (MQ=255)
                                                                                                                      aCCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTa                                               >  4:387452/1‑115 (MQ=255)
                                                                                                                      aCCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTcaca              >  2:77662/1‑148 (MQ=255)
                                                                                                                      aCCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTcaca              >  3:625170/1‑148 (MQ=255)
                                                                                                                      aCCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTcaca              >  2:461875/1‑148 (MQ=255)
                                                                                                                      aCCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTcaca              >  1:697713/1‑148 (MQ=255)
                                                                                                                        cGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCg                                                                         <  3:72863/87‑1 (MQ=255)
                                                                                                                        cGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCg                                                                         >  4:72863/1‑87 (MQ=255)
                                                                                                                        cGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATa            >  2:1067448/1‑148 (MQ=255)
                                                                                                                         gAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATaa           <  1:77662/148‑1 (MQ=255)
                                                                                                                            cACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCg        <  2:697713/148‑1 (MQ=255)
                                                                                                                             aCGGCGCGCTGCACGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGc                                                                        >  3:648242/1‑83 (MQ=255)
                                                                                                                             aCGGCGCGCTGCACGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGc                                                                        <  4:648242/83‑1 (MQ=255)
                                                                                                                                gCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGt    >  3:37012/1‑148 (MQ=255)
                                                                                                                                  gTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGa  <  1:298903/148‑1 (MQ=255)
                                                                                                                                   tGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCg                   >  1:716671/1‑130 (MQ=255)
                                                                                                                                   tGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCg                   <  2:716671/130‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                                  
GCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGA  >  NC_000913/828615‑828892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑