| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 828,752 | G→A | 5.5% | A77V (GCG→GTG) | ybhF ← | putative ABC transporter ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 828,752 | 0 | G | A | 5.5% | 248.6 / 3.3 | 73 | A77V (GCG→GTG) | ybhF | putative ABC transporter ATPase |
| Reads supporting (aligned to +/- strand): ref base G (36/33); new base A (2/2); total (38/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGA > NC_000913/828615‑828892 | gcagGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGGGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGtt > 2:965729/1‑148 (MQ=255) aaaaGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCTAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGa < 1:965729/148‑1 (MQ=255) aaaGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGCGCTGCACGCcg > 2:885471/1‑132 (MQ=255) aaaGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGCGCTGCACGCcg < 1:885471/132‑1 (MQ=255) gTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCaa < 2:797289/148‑1 (MQ=255) tGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTg < 1:1028300/127‑1 (MQ=255) tGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTg > 2:1028300/1‑127 (MQ=255) cTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCg > 2:673928/1‑115 (MQ=255) cTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCg < 1:673928/115‑1 (MQ=255) tACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGc > 2:906258/1‑127 (MQ=255) tACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGc < 1:906258/127‑1 (MQ=255) aCAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCaa < 2:1055026/105‑1 (MQ=255) aCAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCaa > 1:1055026/1‑105 (MQ=255) aCAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTc < 2:1034946/133‑1 (MQ=255) aCAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTc > 1:1034946/1‑133 (MQ=255) aCAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTc > 4:447376/1‑140 (MQ=255) aCAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTc < 3:447376/140‑1 (MQ=255) ttGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGAt < 3:913042/92‑1 (MQ=255) ttGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGAt > 4:913042/1‑92 (MQ=255) ttGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCa > 2:578912/1‑148 (MQ=255) tGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCaa < 2:337883/99‑1 (MQ=255) tGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCaa > 1:337883/1‑99 (MQ=255) ttCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATcc > 2:475435/1‑148 (MQ=255) ttCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATcc > 1:251441/1‑148 (MQ=255) ctcCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGc > 4:424629/1‑148 (MQ=255) cATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATc < 1:578912/148‑1 (MQ=255) aTCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCcgtcg < 3:322580/72‑1 (MQ=255) aTCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCcgtcg > 4:322580/1‑72 (MQ=255) aTCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGtt > 2:346519/1‑74 (MQ=255) aTCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGtt < 1:346519/74‑1 (MQ=255) aTCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCgg < 2:606510/83‑1 (MQ=255) aTCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCgg > 1:606510/1‑83 (MQ=255) gTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTgg < 1:475435/148‑1 (MQ=255) gaTCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGtttt < 2:251441/148‑1 (MQ=255) cTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTAcc < 3:424629/148‑1 (MQ=255) tCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGtt > 3:850177/1‑106 (MQ=255) tCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGtt < 4:850177/106‑1 (MQ=255) aCAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGAt > 3:298893/1‑61 (MQ=255) aCAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGAt < 4:298893/61‑1 (MQ=255) aCAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCAcc > 2:298903/1‑148 (MQ=255) cAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAAt < 2:110906/70‑1 (MQ=255) cAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAAt > 1:110906/1‑70 (MQ=255) aaaTTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTc > 2:155097/1‑97 (MQ=255) aaaTTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTc < 1:155097/97‑1 (MQ=255) aaTTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCa > 1:22135/1‑66 (MQ=255) aaTTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCa < 2:22135/66‑1 (MQ=255) atatAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCaa > 2:390869/1‑98 (MQ=255) atatAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCaa < 1:390869/98‑1 (MQ=255) ataACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATc < 4:136782/106‑1 (MQ=255) ataACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATc > 3:136782/1‑106 (MQ=255) taACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTaa < 2:888555/86‑1 (MQ=255) taACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTaa < 4:178346/86‑1 (MQ=255) taACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTaa > 3:178346/1‑86 (MQ=255) taACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTaa > 1:888555/1‑86 (MQ=255) taACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGc < 2:774632/132‑1 (MQ=255) taACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGc > 1:774632/1‑132 (MQ=255) aCCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTa < 3:387452/115‑1 (MQ=255) aCCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTa > 4:387452/1‑115 (MQ=255) aCCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTcaca > 3:625170/1‑148 (MQ=255) aCCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTcaca > 2:77662/1‑148 (MQ=255) aCCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTcaca > 2:461875/1‑148 (MQ=255) aCCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTcaca > 1:697713/1‑148 (MQ=255) cGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCg < 3:72863/87‑1 (MQ=255) cGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCg > 4:72863/1‑87 (MQ=255) cGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATa > 2:1067448/1‑148 (MQ=255) gAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATaa < 1:77662/148‑1 (MQ=255) cACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCg < 2:697713/148‑1 (MQ=255) aCGGCGCGCTGCACGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGc > 3:648242/1‑83 (MQ=255) aCGGCGCGCTGCACGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGc < 4:648242/83‑1 (MQ=255) gCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGt > 3:37012/1‑148 (MQ=255) gTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGa < 1:298903/148‑1 (MQ=255) tGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCg > 1:716671/1‑130 (MQ=255) tGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCg < 2:716671/130‑1 (MQ=255) | GCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGA > NC_000913/828615‑828892 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |