Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 93,867 C→T 5.5% C234C (TGC→TGT murE → UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate:meso‑ diaminopimelate ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091393,8670CT 5.5% 241.3 / 4.1 72C234C (TGC→TGTmurEUDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate:meso‑ diaminopimelate ligase
Reads supporting (aligned to +/- strand):  ref base C (33/35);  new base T (2/2);  total (35/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.24e-01

CTCCCACGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTAT  >  NC_000913/93720‑94008
                                                                                                                                                   |                                                                                                                                             
ctccCACGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGc                                                                                                                                               <  3:1041843/148‑1 (MQ=255)
    cACGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTc                                                                                                                                           >  1:629471/1‑148 (MQ=255)
     aCGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCTCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCa                                                                                                                                          >  3:739450/1‑148 (MQ=255)
       gggCTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAgg                                                                                                                                        <  2:743153/148‑1 (MQ=255)
          cTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGa                                                                                                                                     <  4:841317/148‑1 (MQ=255)
              tACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGattat                                                                                                                                 >  4:247375/1‑148 (MQ=255)
                cAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTCTACCAACTTAAGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGTCGAttatta                                                                                                                               <  4:739450/148‑1 (MQ=255)
                 aGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTaa                                                                                                                              <  2:522299/148‑1 (MQ=255)
                   cACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGCCGCGATCACCTTGATTATCATGGTGCTATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGc                                                                                                                                               >  2:367654/1‑129 (MQ=255)
                   cACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGc                                                                                                                                               <  1:367654/129‑1 (MQ=255)
                          tgGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCcgacga                                                                                                                     <  2:629471/148‑1 (MQ=255)
                               gcATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAg                                                                                                                <  3:247375/148‑1 (MQ=255)
                                                 tCGGTCTTTACCAACTTAAGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGctggctgg                                                                                              >  1:288909/1‑148 (MQ=255)
                                                      cTTTACCAACTTAAGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAgg                                                                                                                                        >  4:442641/1‑101 (MQ=255)
                                                      cTTTACCAACTTAAGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAgg                                                                                                                                        <  3:442641/101‑1 (MQ=255)
                                                      cTTTACCAACTTAAGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTaa                                                                                                                              >  1:373632/1‑111 (MQ=255)
                                                      cTTTACCAACTTAAGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTaa                                                                                                                              <  2:373632/111‑1 (MQ=255)
                                                        ttACCAACTTAAGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATGAACGCCGAAGATGAAGTGGGCCGCCGCTGGCTGGCAAAACt                                                                                       >  4:934975/1‑148 (MQ=255)
                                                          aCCAACTTAAGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGt                                                                                                               >  3:345479/1‑122 (MQ=255)
                                                          aCCAACTTAAGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGt                                                                                                               <  4:345479/122‑1 (MQ=255)
                                                            cAACTTAAGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGcc                                                                                                                          >  3:33112/1‑109 (MQ=255)
                                                            cAACTTAAGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGcc                                                                                                                          <  4:33112/109‑1 (MQ=255)
                                                            cAACTTAAGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAACTGCCg                                                                                   >  2:1079000/1‑148 (MQ=255)
                                                                ttAAGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTaa                                                                                                                              <  3:1112661/101‑1 (MQ=255)
                                                                ttAAGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTaa                                                                                                                              >  4:1112661/1‑101 (MQ=255)
                                                                  aaGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAACTGCCGGAcgcg                                                                             <  2:288909/148‑1 (MQ=255)
                                                                                ttGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGa                                                                                                                  >  3:280408/1‑97 (MQ=255)
                                                                                ttGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGa                                                                                                                  <  4:280408/97‑1 (MQ=255)
                                                                                ttGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGgccg                                                                                                         <  2:590957/106‑1 (MQ=255)
                                                                                ttGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGgccg                                                                                                         >  1:590957/1‑106 (MQ=255)
                                                                                ttGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCgctggc                                                                                                 <  2:272897/114‑1 (MQ=255)
                                                                                ttGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCgctggc                                                                                                 >  1:272897/1‑114 (MQ=255)
                                                                                 tGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACg                                                                                                                            >  3:936815/1‑86 (MQ=255)
                                                                                 tGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACg                                                                                                                            <  4:936815/86‑1 (MQ=255)
                                                                                 tGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATg                                                              >  2:551735/1‑148 (MQ=255)
                                                                                   actATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGa                                                            <  3:934975/146‑1 (MQ=255)
                                                                                   aTTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGa                                                                                                                                     >  3:1036126/1‑75 (MQ=255)
                                                                                   aTTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGa                                                                                                                                     <  4:1036126/75‑1 (MQ=255)
                                                                                     tATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGTGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAg                                                          >  3:1085249/1‑148 (MQ=255)
                                                                                     tATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGTGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAg                                                          >  1:855425/1‑148 (MQ=255)
                                                                                       tCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGAt                                                        <  1:551735/148‑1 (MQ=255)
                                                                                          tGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGTGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCat                                                     <  4:1085249/148‑1 (MQ=255)
                                                                                          tGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGTGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCat                                                     <  2:855425/148‑1 (MQ=255)
                                                                                             tGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTg                                                                                                              <  3:342061/88‑1 (MQ=255)
                                                                                             tGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTg                                                                                                              >  4:1091709/1‑88 (MQ=255)
                                                                                             tGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTg                                                                                                              >  4:342061/1‑88 (MQ=255)
                                                                                             tGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTg                                                                                                              <  3:1091709/88‑1 (MQ=255)
                                                                                               atatGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCg                                                                                                                                              <  2:786443/54‑1 (MQ=255)
                                                                                               atatGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCg                                                                                                                                              >  1:786443/1‑54 (MQ=255)
                                                                                                 atGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCcga                                                                                                                        >  3:384413/1‑74 (MQ=255)
                                                                                                 atGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCcga                                                                                                                        <  4:384413/74‑1 (MQ=255)
                                                                                                 atGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAg                                                                                                                <  2:260110/82‑1 (MQ=255)
                                                                                                 atGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAg                                                                                                                >  1:260110/1‑82 (MQ=255)
                                                                                                 atGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTa                                                                    >  2:783578/1‑126 (MQ=255)
                                                                                                 atGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTa                                                                    <  1:783578/126‑1 (MQ=255)
                                                                                                   ggAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCat                                                     <  3:38686/139‑1 (MQ=255)
                                                                                                   ggAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCat                                                     >  4:38686/1‑139 (MQ=255)
                                                                                                    gAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGa                                           <  1:1079000/148‑1 (MQ=255)
                                                                                                         cTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGc                                                                                                                                               >  2:553993/1‑43 (MQ=255)
                                                                                                         cTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGc                                                                                                                                               <  1:553993/43‑1 (MQ=255)
                                                                                                                          ggCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGaa                     >  4:598693/1‑148 (MQ=255)
                                                                                                                             tgctTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGa                                           <  4:116092/123‑1 (MQ=255)
                                                                                                                             tgctTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGa                                           >  3:116092/1‑123 (MQ=255)
                                                                                                                              gctTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCg                 >  4:1026915/1‑148 (MQ=255)
                                                                                                                                tttATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAGCTGCCGGACGCTGTTGCGGTATCAATGGAAGATCata                                                    <  2:759370/111‑1 (MQ=255)
                                                                                                                                tttATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAGCTGCCGGACGCGGTTGCGGTATCAATGGAAGATCata                                                    >  1:759370/1‑111 (MQ=255)
                                                                                                                                 ttATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTg                                                                                                              <  1:385232/52‑1 (MQ=255)
                                                                                                                                 ttATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTg                                                                                                              >  2:385232/1‑52 (MQ=255)
                                                                                                                                 ttATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGAcc              <  3:598693/148‑1 (MQ=255)
                                                                                                                                     tCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAg          >  2:973212/1‑148 (MQ=255)
                                                                                                                                        gAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGa                >  3:1046689/1‑139 (MQ=255)
                                                                                                                                        gAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGa                <  4:1046689/139‑1 (MQ=255)
                                                                                                                                           catcatTGCGGTCAGGCGATTATTAACGCCGACGAt                                                                                                                    >  2:755342/1‑36 (MQ=255)
                                                                                                                                           catcatTGCGGTCAGGCGATTATTAACGCCGACGAt                                                                                                                    <  1:755342/36‑1 (MQ=255)
                                                                                                                                             tcatTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTAt  <  3:1026915/148‑1 (MQ=255)
                                                                                                                                                 tGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGGAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGa       >  2:213284/1‑139 (MQ=255)
                                                                                                                                                 tGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCAGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGa       <  1:213284/139‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
CTCCCACGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGCCGCGATCACCTTGATTATCATGGTGATATGGAACACTACGAAGCCGCGAAATGGCTGCTTTATTCTGAGCATCATTGCGGTCAGGCGATTATTAACGCCGACGATGAAGTGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTAT  >  NC_000913/93720‑94008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: