Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 996,662 C→A 6.7% G95W (GGG→TGG)  ssuA ← aliphatic sulfonate ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913996,6620CA 6.7% 316.5 / 11.2 90G95W (GGG→TGG) ssuAaliphatic sulfonate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (43/41);  new base A (3/3);  total (46/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.01e-01

GGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGT  >  NC_000913/996531‑996808
                                                                                                                                   |                                                                                                                                                  
ggAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAg                                                                                                                                    >  2:384679/1‑148 (MQ=255)
  aaCCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTgg                                                                                                                                          >  3:836504/1‑140 (MQ=255)
  aaCCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTgg                                                                                                                                          <  4:836504/140‑1 (MQ=255)
  aaCCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATAGGGCTGTTTTCTGCCACCAGAATCACTTCGCCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGAt                                                                                                                                  >  2:330543/1‑148 (MQ=255)
           gAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCt                                                                                                                                            <  1:141235/129‑1 (MQ=255)
           gAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCt                                                                                                                                            >  2:141235/1‑129 (MQ=255)
            aaaGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAata                                                                                                                        <  1:384679/148‑1 (MQ=255)
             aaGGCAACTTTGTGACCTTTAAGATCGGATACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAatat                                                                                                                       >  1:552148/1‑148 (MQ=255)
                gCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATccc                                                                                                                    <  3:970570/148‑1 (MQ=255)
                   aCTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGAtt                                                                                                                                 <  3:107734/132‑1 (MQ=255)
                   aCTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGAtt                                                                                                                                 >  4:107734/1‑132 (MQ=255)
                     tttGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTa                                                                                                               >  4:259988/1‑148 (MQ=255)
                      ttGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTAc                                                                                                              >  2:999239/1‑148 (MQ=255)
                           aCCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCTGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCg                                                                                                         <  1:330543/148‑1 (MQ=255)
                               ttAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTg                                                                                                                                           >  1:602475/1‑110 (MQ=255)
                               ttAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTg                                                                                                                                           <  2:602475/110‑1 (MQ=255)
                               ttAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGAt                                                                                                     >  2:420819/1‑148 (MQ=255)
                                   gATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCaa                                                                                                                                      >  3:226140/1‑111 (MQ=255)
                                   gATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCaa                                                                                                                                      <  4:226140/111‑1 (MQ=255)
                                    aTCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCaa                                                                                                                                      >  1:788595/1‑110 (MQ=255)
                                    aTCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCaa                                                                                                                                      <  2:788595/110‑1 (MQ=255)
                                             ggTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACg                                                                                       <  1:420819/148‑1 (MQ=255)
                                                tttGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCa                                                                                                   >  4:285931/1‑133 (MQ=255)
                                                tttGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCa                                                                                                   <  3:285931/133‑1 (MQ=255)
                                                 ttGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCg                                                                                                         >  3:236062/1‑126 (MQ=255)
                                                 ttGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCg                                                                                                         <  4:236062/126‑1 (MQ=255)
                                                 ttGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTa                                                                                   <  2:552148/148‑1 (MQ=255)
                                                 ttGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTa                                                                                   <  1:999239/148‑1 (MQ=255)
                                                     tCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCg                                                                                                         >  1:206343/1‑122 (MQ=255)
                                                     tCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAAGCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCg                                                                                                         <  2:206343/122‑1 (MQ=255)
                                                      cGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCt                                                                              <  3:259988/148‑1 (MQ=255)
                                                          cTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCa                                                                          >  1:303106/1‑148 (MQ=255)
                                                                     caccaGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTc                                                                            >  4:907576/1‑135 (MQ=255)
                                                                     caccaGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTc                                                                            <  3:907576/135‑1 (MQ=255)
                                                                        caGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTgg                                                                                                                               <  3:794963/81‑1 (MQ=255)
                                                                        caGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTgg                                                                                                                               >  4:794963/1‑81 (MQ=255)
                                                                            aTCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCgg                                                        <  2:303106/148‑1 (MQ=255)
                                                                              cACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGc                                                                                                                              <  2:972316/76‑1 (MQ=255)
                                                                              cACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGc                                                                                                                              >  1:972316/1‑76 (MQ=255)
                                                                              cACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATccc                                                                                                                    <  3:910374/86‑1 (MQ=255)
                                                                              cACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATccc                                                                                                                    >  4:910374/1‑86 (MQ=255)
                                                                                  tCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGAt                                                                                                     <  1:538878/97‑1 (MQ=255)
                                                                                  tCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGAt                                                                                                     >  2:538878/1‑97 (MQ=255)
                                                                                  tCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCa                                                                          >  2:25890/1‑124 (MQ=255)
                                                                                  tCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCa                                                                          <  1:25890/124‑1 (MQ=255)
                                                                                  tCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTg                                                                   <  3:552594/131‑1 (MQ=255)
                                                                                  tCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTg                                                                   >  4:552594/1‑131 (MQ=255)
                                                                                       tttGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCAcc                                             >  3:528993/1‑148 (MQ=255)
                                                                                        ttGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGaa                                                                                                                          <  1:663480/70‑1 (MQ=255)
                                                                                        ttGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGaa                                                                                                                          >  2:663480/1‑70 (MQ=255)
                                                                                        ttGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCcg                                                           <  3:382092/133‑1 (MQ=255)
                                                                                        ttGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCcg                                                           >  4:382092/1‑133 (MQ=255)
                                                                                        ttGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCAccc                                            >  2:949050/1‑148 (MQ=255)
                                                                                           ggCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTg                                                                   >  3:31704/1‑122 (MQ=255)
                                                                                           ggCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTg                                                                   <  4:31704/122‑1 (MQ=255)
                                                                                               tCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCg                                                                                                         <  2:68629/80‑1 (MQ=255)
                                                                                               tCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCg                                                                                                         >  1:68629/1‑80 (MQ=255)
                                                                                                       tCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTc                                                 >  3:61132/1‑128 (MQ=255)
                                                                                                       tCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTc                                                 <  4:61132/128‑1 (MQ=255)
                                                                                                          aCGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGAttt                                   >  4:1028983/1‑139 (MQ=255)
                                                                                                          aCGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGAttt                                   <  3:1028983/139‑1 (MQ=255)
                                                                                                               cACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACt                                                                                                             >  4:7683/1‑60 (MQ=255)
                                                                                                               cACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACt                                                                                                             <  3:7683/60‑1 (MQ=255)
                                                                                                                 cGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAAc                                                                        >  3:48618/1‑95 (MQ=255)
                                                                                                                 cGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAAc                                                                        <  4:48618/95‑1 (MQ=255)
                                                                                                                     cacCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGtttt               >  2:159078/1‑148 (MQ=255)
                                                                                                                      acCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGttttt              <  1:949050/148‑1 (MQ=255)
                                                                                                                       ccAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATc                                                                                                                      <  3:188204/43‑1 (MQ=255)
                                                                                                                       ccAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATc                                                                                                                      >  4:188204/1‑43 (MQ=255)
                                                                                                                       ccAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCgg                         >  2:832753/1‑136 (MQ=255)
                                                                                                                       ccAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCgg                         <  1:832753/136‑1 (MQ=255)
                                                                                                                       ccAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTc             >  1:941743/1‑148 (MQ=255)
                                                                                                                        cAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATc                                                                                                    <  3:844799/60‑1 (MQ=255)
                                                                                                                        cAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATc                                                                                                    >  4:844799/1‑60 (MQ=255)
                                                                                                                        cAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTa                                                                                   >  3:1144515/1‑77 (MQ=255)
                                                                                                                        cAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTa                                                                                   <  4:1144515/77‑1 (MQ=255)
                                                                                                                        cAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGAttt                                   >  3:263240/1‑125 (MQ=255)
                                                                                                                        cAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGAttt                                   <  4:263240/125‑1 (MQ=255)
                                                                                                                        cAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTcc            <  1:159078/148‑1 (MQ=255)
                                                                                                                          aaTCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTaa                                                                                  >  4:48075/1‑76 (MQ=255)
                                                                                                                          aaTCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTaa                                                                                  <  3:48075/76‑1 (MQ=255)
                                                                                                                           aTCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGgag                                       >  3:329704/1‑118 (MQ=255)
                                                                                                                           aTCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGgag                                       <  4:329704/118‑1 (MQ=255)
                                                                                                                            tCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTa        >  2:832780/1‑148 (MQ=255)
                                                                                                                             ttgCCCAGGCTGCCGGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTg                                                                   <  1:80462/86‑1 (MQ=255)
                                                                                                                             ttgCCCAGGCTGCCGGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTg                                                                   >  2:80462/3‑88 (MQ=255)
                                                                                                                             ttgCCCAGGCTGCCGGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCa           <  3:825142/142‑1 (MQ=255)
                                                                                                                             ttgCCCAGGCTGCCGGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCa           >  4:825142/3‑144 (MQ=255)
                                                                                                                             ttgCCCAGGCTGCCGGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCa           >  4:1120437/3‑144 (MQ=255)
                                                                                                                             ttgCCCAGGCTGCCGGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCa           <  3:1120437/142‑1 (MQ=255)
                                                                                                                                 cccGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCgg                                                                >  1:502130/1‑87 (MQ=255)
                                                                                                                                 cccGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCgg                                                                <  2:502130/87‑1 (MQ=255)
                                                                                                                                 cccGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGAt                       >  4:251067/1‑128 (MQ=255)
                                                                                                                                 cccGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGAt                       <  3:251067/128‑1 (MQ=255)
                                                                                                                                 cccGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACtgg   >  3:516560/1‑148 (MQ=255)
                                                                                                                                  ccGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACg                                                                                       <  4:621755/63‑1 (MQ=255)
                                                                                                                                  ccGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACg                                                                                       >  3:621755/1‑63 (MQ=255)
                                                                                                                                  ccGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGAttt                                   >  2:926807/1‑115 (MQ=255)
                                                                                                                                  ccGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGAttt                                   <  1:926807/115‑1 (MQ=255)
                                                                                                                                  ccGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACtggt  >  2:302468/1‑148 (MQ=255)
                                                                                                                                   |                                                                                                                                                  
GGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGT  >  NC_000913/996531‑996808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: