Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,116,854 A→C 8.3% K16N (AAA→AAC yceA → putative rhodanese‑related sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,116,8540AC 8.3% 318.6 / 17.2 95K16N (AAA→AACyceAputative rhodanese‑related sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base A (45/42);  new base C (3/5);  total (48/47)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

GTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCAT  >  NC_000913/1116718‑1117000
                                                                                                                                        |                                                                                                                                                  
gTAGCGAAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCCCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTga                                                                                                                                         >  2:106694/1‑148 (MQ=255)
     gTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTCTCAGCCAACATTTTGGCttttagcg                                                                                                                                    <  3:887999/148‑8 (MQ=255)
                  aTGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAgcgaaccg                                                                                                                               <  3:882934/140‑1 (MQ=255)
                  aTGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGGGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAgcgaaccg                                                                                                                               >  4:882934/1‑140 (MQ=255)
                     ttAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCAt                                                                                                                    >  3:945188/1‑148 (MQ=255)
                      tAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAACATTTTGGCt                                                                                                                                           <  2:81783/124‑1 (MQ=255)
                      tAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAACATTTTGGCt                                                                                                                                           >  1:81783/1‑124 (MQ=255)
                      tAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGtt                                                                                                                                               <  4:23667/120‑1 (MQ=255)
                      tAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGtt                                                                                                                                               <  3:980716/120‑1 (MQ=255)
                      tAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGtt                                                                                                                                               >  3:23667/1‑120 (MQ=255)
                      tAATTATCCATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGta                                                                                                                                               >  4:980716/1‑119 (MQ=255)
                       aaTTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTgag                                                                                                                                        <  2:467529/126‑1 (MQ=255)
                        aTTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAgcga                                                                                                                                   <  3:401264/130‑1 (MQ=255)
                        aTTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAgcga                                                                                                                                   >  4:401264/1‑130 (MQ=255)
                                tAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACa                                                                                                         <  1:106694/148‑1 (MQ=255)
                                  aTTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTgag                                                                                                                                        >  4:467181/1‑115 (MQ=255)
                                     gCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTAt                                                                                                    <  4:945188/148‑1 (MQ=255)
                                              tGCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAATAGCCaaa                                                                                                                                                    <  2:365695/91‑1 (MQ=255)
                                              tGCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCaaa                                                                                                                                                    >  1:365695/1‑91 (MQ=255)
                                               gCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCACAATGtt                                                                                                                                               >  4:1105040/1‑95 (MQ=255)
                                               gCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGtt                                                                                                                                               <  3:1105040/95‑1 (MQ=255)
                                                  gcgGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAgcgaa                                                                                                                                  >  3:837354/1‑105 (MQ=255)
                                                  gcgGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAgcgaa                                                                                                                                  <  4:837354/105‑1 (MQ=255)
                                                   cgGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAAccacca                                                                                                                     <  3:17022/117‑1 (MQ=255)
                                                               ttCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAACAtt                                                                                                                                                 <  3:535189/77‑2 (MQ=255)
                                                               ttCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAACAtt                                                                                                                                                 >  4:535189/1‑76 (MQ=255)
                                                                   cATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGt                                                                                                                                                <  1:81819/74‑1 (MQ=255)
                                                                   cATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTgag                                                                                                                                        >  4:557708/1‑82 (MQ=255)
                                                                   cATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTgag                                                                                                                                        <  3:557708/82‑1 (MQ=255)
                                                                               aCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTc                                                                                                 <  3:1047172/109‑1 (MQ=255)
                                                                                       ccATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGt                                                                                                               >  3:991179/1‑87 (MQ=255)
                                                                                          tGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGGGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTAc                                               >  4:345331/1‑148 (MQ=255)
                                                                                          tGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTAttt                                                                                                  <  2:85734/97‑1 (MQ=255)
                                                                                          tGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTg                                                                     <  3:1082899/126‑1 (MQ=255)
                                                                                          tGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTg                                                                     >  4:1082899/1‑126 (MQ=255)
                                                                                          tGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTAc                                               >  2:202557/1‑148 (MQ=255)
                                                                                           gCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTaa                                                                                >  2:403634/1‑114 (MQ=255)
                                                                                           gCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTaa                                                                                <  1:403634/114‑1 (MQ=255)
                                                                                              aGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACGAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCttt                                                              >  2:941925/1‑129 (MQ=255)
                                                                                              aGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACGAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCttt                                                              <  1:941925/129‑1 (MQ=255)
                                                                                              aGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACcgcg                                           >  4:159602/1‑148 (MQ=255)
                                                                                                  ttACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGa                                       <  3:799114/148‑1 (MQ=255)
                                                                                                   tACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAgg                                                                              >  1:879478/1‑108 (MQ=255)
                                                                                                   tACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAgg                                                                              <  2:879478/108‑1 (MQ=255)
                                                                                                     cacaACCGCATTTCCAACGACGCGCTAAAAGCCaaa                                                                                                                                                    >  2:145721/1‑36 (MQ=255)
                                                                                                     cacaACCGCATTTCCAACGACGCGCTAAAAGCCaaa                                                                                                                                                    <  1:145721/36‑1 (MQ=255)
                                                                                                     cacaACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCtt                                                               <  1:678285/121‑1 (MQ=255)
                                                                                                     cacaACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCtt                                                               >  2:678285/1‑121 (MQ=255)
                                                                                                      acaACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTg                                                                                                                                              <  4:764371/41‑1 (MQ=255)
                                                                                                      acaACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTg                                                                                                                                              >  3:764371/1‑41 (MQ=255)
                                                                                                      acaACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGtt                                                  <  1:139264/133‑1 (MQ=255)
                                                                                                       caACCGCATTTCCAACGACGCGCTAAAAGCCATAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGtt                                  <  3:345331/148‑1 (MQ=255)
                                                                                                       caACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGtt                                  <  1:202557/148‑1 (MQ=255)
                                                                                                         aCCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATc                                                         <  1:983776/123‑1 (MQ=255)
                                                                                                         aCCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATc                                                         >  2:983776/1‑123 (MQ=255)
                                                                                                           cGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACa                                                                                                         >  4:1063201/1‑73 (MQ=255)
                                                                                                            gCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCt                                         <  2:167222/136‑1 (MQ=255)
                                                                                                            gCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCt                                         >  1:167222/1‑136 (MQ=255)
                                                                                                              aTTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGc                           <  3:159602/148‑1 (MQ=255)
                                                                                                               tttCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGAt                                                                                     >  3:265183/1‑89 (MQ=255)
                                                                                                               tttCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGAt                                                                                     <  4:265183/89‑1 (MQ=255)
                                                                                                                ttCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTg                              <  1:457562/143‑1 (MQ=255)
                                                                                                                ttCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTg                              >  2:457562/1‑143 (MQ=255)
                                                                                                                    aaCGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGt                                                                                                               <  3:1127791/58‑1 (MQ=255)
                                                                                                                    aaCGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGt                                                                                                               >  4:1127791/1‑58 (MQ=255)
                                                                                                                      cgacgCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAg                                                                               >  3:540621/1‑88 (MQ=255)
                                                                                                                      cgacgCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAg                                                                               <  4:540621/88‑1 (MQ=255)
                                                                                                                        acgCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCt                 >  2:489802/1‑148 (MQ=255)
                                                                                                                             cTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGgcgc            >  1:37516/1‑148 (MQ=255)
                                                                                                                              tAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTAtt                                                                                                   >  3:391912/1‑60 (MQ=255)
                                                                                                                              tAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTAtt                                                                                                   <  4:391912/60‑1 (MQ=255)
                                                                                                                              tAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTg                              <  4:825172/129‑1 (MQ=255)
                                                                                                                                 aaGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTggg                            <  4:241544/128‑1 (MQ=255)
                                                                                                                                   gCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGtttt                                >  1:296118/1‑122 (MQ=255)
                                                                                                                                   gCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGtttt                                <  2:296118/122‑1 (MQ=255)
                                                                                                                                    ccAACATTTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATg         >  2:87613/1‑144 (MQ=255)
                                                                                                                                    ccAACATTTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATg         <  1:87613/144‑1 (MQ=255)
                                                                                                                                    ccAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTtat                                                            >  1:121692/1‑93 (MQ=255)
                                                                                                                                    ccAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTtat                                                            >  1:554228/1‑93 (MQ=255)
                                                                                                                                    ccAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTtat                                                            <  2:121692/93‑1 (MQ=255)
                                                                                                                                    ccAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTtat                                                            <  2:554228/93‑1 (MQ=255)
                                                                                                                                    ccAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAggg     >  1:281761/1‑148 (MQ=255)
                                                                                                                                     cAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCt                                                                >  2:805076/1‑88 (MQ=255)
                                                                                                                                     cAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCt                                                                <  1:459273/88‑1 (MQ=255)
                                                                                                                                     cAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCt                                                                <  1:805076/88‑1 (MQ=255)
                                                                                                                                      aaaaTGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTg                                                    <  3:62766/99‑1 (MQ=255)
                                                                                                                                      aaaaTGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTg                                                    >  4:62766/1‑99 (MQ=255)
                                                                                                                                      aaaaTGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTg                                                    >  2:984190/1‑99 (MQ=255)
                                                                                                                                      aaaaTGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTg                                                    <  1:984190/99‑1 (MQ=255)
                                                                                                                                      aaaaTGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCa   <  1:489802/148‑1 (MQ=255)
                                                                                                                                       aaaTGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATg                                    >  4:468784/1‑114 (MQ=255)
                                                                                                                                       aaaTGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATg                                    <  3:468784/114‑1 (MQ=255)
                                                                                                                                       aaaTGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTggg                            >  3:466355/1‑122 (MQ=255)
                                                                                                                                       aaaTGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCg                          >  3:1112989/1‑124 (MQ=255)
                                                                                                                                       aaaTGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCg                          <  4:1112989/124‑1 (MQ=255)
                                                                                                                                       aaaTGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTg                <  3:94743/134‑1 (MQ=255)
                                                                                                                                       aaaTGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCAt  >  1:199460/1‑148 (MQ=255)
                                                                                                                                       aaaTGTTAGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTg                >  4:94743/1‑134 (MQ=255)
                                                                                                                                        aaTGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATg         >  4:380788/1‑140 (MQ=255)
                                                                                                                                        aaTGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATg         <  3:380788/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                                  
GTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCAT  >  NC_000913/1116718‑1117000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: