Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,147,986 G→C 13.2% L122L (CTG→CTC)  plsX → putative phosphate acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,147,9860GC13.2% 206.6 / 21.7 68L122L (CTG→CTC) plsXputative phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (29/30);  new base C (4/5);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

CCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGA  >  NC_000913/1147839‑1148132
                                                                                                                                                   |                                                                                                                                                  
ccGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTg                                                                                                                                                    >  4:197562/1‑148 (MQ=255)
  ggCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGa                                                                                                                                                  <  1:682679/148‑1 (MQ=255)
         gCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCtt                                                                                                                                           <  1:1020785/148‑1 (MQ=255)
          cAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTg                                                                                                                                          <  3:197562/148‑1 (MQ=255)
                              tGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACg                                                                                                                      >  1:200136/1‑148 (MQ=255)
                                     tCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCTTg                                                                                                                                          >  4:997688/1‑121 (MQ=255)
                                     tCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCTTg                                                                                                                                          <  3:997688/121‑1 (MQ=255)
                                     tCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCg                                                                                                                                      <  3:1086594/125‑1 (MQ=255)
                                     tCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCAGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCg                                                                                                                                      >  4:1086594/1‑125 (MQ=255)
                                             cgTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCTTg                                                                                                                                          <  1:1038572/113‑1 (MQ=255)
                                             cgTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCTTg                                                                                                                                          >  2:1038572/1‑113 (MQ=255)
                                                     tGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAgggg                                                                                                                                              <  3:251322/101‑1 (MQ=255)
                                                     tGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAgggg                                                                                                                                              >  4:251322/1‑101 (MQ=255)
                                                            ggTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTAcc                                                                                                              <  3:166741/126‑1 (MQ=255)
                                                            ggTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTAcc                                                                                                              >  4:166741/1‑126 (MQ=255)
                                                             gTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCTTg                                                                                                                                          >  3:361201/1‑97 (MQ=255)
                                                             gTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCTTg                                                                                                                                          <  4:361201/97‑1 (MQ=255)
                                                             atctaagaagGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGATGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACgg                                                                                       <  1:400044/144‑1 (MQ=255)
                                                              tGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCTTg                                                                                                                                          >  3:183792/1‑96 (MQ=255)
                                                              tGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCTTg                                                                                                                                          <  4:183792/96‑1 (MQ=255)
                                                                 aagaagGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAg                                                                                                 >  3:533686/1‑134 (MQ=255)
                                                                 aagaagGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAg                                                                                                 <  4:533686/134‑1 (MQ=255)
                                                                  agaagGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTc                                                                                  <  2:200136/148‑1 (MQ=255)
                                                                  agaagGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTc                                                                                  <  1:620018/148‑1 (MQ=255)
                                                                   gaagGTCGAGCGCAAGCCTGTGTCAGTGCCGTTAATACCGGGGCGCTGATTGTTCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTTAGCTTCGGACGCTGGTGTCGGGATTACCACATCAGCAAAGGTGCAAAACggtgggat                                                                                 >  2:400044/1‑145 (MQ=255)
                                                                   gaagGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAg                                                                                                                                                 >  3:554368/1‑84 (MQ=255)
                                                                   gaagGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAg                                                                                                                                                 <  4:554368/84‑1 (MQ=255)
                                                                   gaagGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCt                                                                                                                             >  1:498756/1‑104 (MQ=255)
                                                                   gaagGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCt                                                                                                                             <  2:498756/104‑1 (MQ=255)
                                                                         cGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACggt                                                                                      <  4:908224/137‑1 (MQ=255)
                                                                         cGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACggt                                                                                      >  3:908224/1‑137 (MQ=255)
                                                                                   ccTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTAGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGGAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCaa                                                                 >  3:740373/1‑148 (MQ=255)
                                                                                         tCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGACAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGa                                                           <  4:740373/148‑1 (MQ=255)
                                                                                          cAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTgg                                                                                                                           >  4:665243/1‑83 (MQ=255)
                                                                                          cAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTgg                                                                                                                           <  3:665243/83‑1 (MQ=255)
                                                                                                                cTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGAt                                                                                                                                            <  4:1041840/44‑1 (MQ=255)
                                                                                                                cTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGAt                                                                                                                                            >  3:1041840/1‑44 (MQ=255)
                                                                                                                    tGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGa                                                           <  1:1070870/121‑1 (MQ=255)
                                                                                                                    tGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGa                                                           >  2:1070870/1‑121 (MQ=255)
                                                                                                                      gggCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTg                              >  3:610653/1‑148 (MQ=255)
                                                                                                                        gCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGAc                                                                                                                       <  3:533168/57‑1 (MQ=255)
                                                                                                                        gCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGAc                                                                                                                       >  4:533168/1‑57 (MQ=255)
                                                                                                                            gCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTa                        >  4:447276/1‑148 (MQ=255)
                                                                                                                             cAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTAt                       <  4:610653/148‑1 (MQ=255)
                                                                                                                                aaTTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATggg                    <  3:447276/148‑1 (MQ=255)
                                                                                                                                  ttattaCTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTgg                                       >  2:910226/1‑127 (MQ=255)
                                                                                                                                  ttattaCTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTgg                                       <  1:910226/127‑1 (MQ=255)
                                                                                                                                   tattaCTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCaa                                  <  3:1050858/131‑1 (MQ=255)
                                                                                                                                   tattaCTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCaa                                  >  4:1050858/1‑131 (MQ=255)
                                                                                                                                      taCTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACgg                                                                                       <  3:328135/75‑1 (MQ=255)
                                                                                                                                      taCTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACgg                                                                                       >  4:328135/1‑75 (MQ=255)
                                                                                                                                      taCTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATtg                                                        >  2:162510/1‑106 (MQ=255)
                                                                                                                                      taCTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATtg                                                        <  1:162510/106‑1 (MQ=255)
                                                                                                                                          cAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTa                                                                                                                >  3:453579/1‑46 (MQ=255)
                                                                                                                                          cAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTa                                                                                                                <  4:453579/46‑1 (MQ=255)
                                                                                                                                          cAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAgg                                                                       >  1:429884/1‑87 (MQ=255)
                                                                                                                                          cAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAgg                                                                       <  2:429884/87‑1 (MQ=255)
                                                                                                                                          cAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTg          >  1:774672/1‑148 (MQ=255)
                                                                                                                                            aGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATc                                                                                                         >  4:503639/1‑51 (MQ=255)
                                                                                                                                            aGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATc                                                                                                         <  3:503639/51‑1 (MQ=255)
                                                                                                                                            aGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCTATtgtg                                                      <  4:288621/102‑1 (MQ=255)
                                                                                                                                            aGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATtgtg                                                      >  3:288621/1‑102 (MQ=255)
                                                                                                                                            aGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGc        >  4:515246/1‑148 (MQ=255)
                                                                                                                                            aGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGc        >  2:952850/1‑148 (MQ=255)
                                                                                                                                              ccccTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAg                                                                                                 <  2:199940/57‑1 (MQ=255)
                                                                                                                                              ccccTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAg                                                                                                 >  1:199940/1‑57 (MQ=255)
                                                                                                                                                 cTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATtg                                                        <  2:531051/95‑1 (MQ=255)
                                                                                                                                                 cTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATtg                                                        >  1:531051/1‑95 (MQ=255)
                                                                                                                                                  tGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAaga  <  3:515246/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGA  >  NC_000913/1147839‑1148132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑