Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,147,993 A→C 10.8% I125L (ATT→CTT)  plsX → putative phosphate acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,147,9930AC10.8% 264.6 / 20.7 83I125L (ATT→CTT) plsXputative phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (38/36);  new base C (4/5);  total (42/41)
Fisher's exact test for biased strand distribution p-value = 7.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

GCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGT  >  NC_000913/1147848‑1148138
                                                                                                                                                 |                                                                                                                                                 
gCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCtt                                                                                                                                                 <  1:1020785/148‑1 (MQ=255)
 cAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTg                                                                                                                                                <  3:197562/148‑1 (MQ=255)
                     tGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACg                                                                                                                            >  1:200136/1‑148 (MQ=255)
                            tCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCTTg                                                                                                                                                >  4:997688/1‑121 (MQ=255)
                            tCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCTTg                                                                                                                                                <  3:997688/121‑1 (MQ=255)
                            tCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCg                                                                                                                                            <  3:1086594/125‑1 (MQ=255)
                            tCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCAGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCg                                                                                                                                            >  4:1086594/1‑125 (MQ=255)
                                    cgTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCTTg                                                                                                                                                >  2:1038572/1‑113 (MQ=255)
                                    cgTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCTTg                                                                                                                                                <  1:1038572/113‑1 (MQ=255)
                                                   ggTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTAcc                                                                                                                    <  3:166741/126‑1 (MQ=255)
                                                   ggTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTAcc                                                                                                                    >  4:166741/1‑126 (MQ=255)
                                                    gTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCTTg                                                                                                                                                <  4:361201/97‑1 (MQ=255)
                                                    gTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCTTg                                                                                                                                                >  3:361201/1‑97 (MQ=255)
                                                    atctaagaagGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGATGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACgg                                                                                             <  1:400044/144‑1 (MQ=255)
                                                     tGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCTTg                                                                                                                                                <  4:183792/96‑1 (MQ=255)
                                                     tGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCTTg                                                                                                                                                >  3:183792/1‑96 (MQ=255)
                                                        aagaagGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAg                                                                                                       <  4:533686/134‑1 (MQ=255)
                                                        aagaagGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAg                                                                                                       >  3:533686/1‑134 (MQ=255)
                                                         agaagGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTc                                                                                        <  1:620018/148‑1 (MQ=255)
                                                         agaagGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTc                                                                                        <  2:200136/148‑1 (MQ=255)
                                                          gaagGTCGAGCGCAAGCCTGTGTCAGTGCCGTTAATACCGGGGCGCTGATTGTTCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTTAGCTTCGGACGCTGGTGTCGGGATTACCACATCAGCAAAGGTGCAAAACggtgggat                                                                                       >  2:400044/1‑145 (MQ=255)
                                                          gaagGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCt                                                                                                                                   <  2:498756/104‑1 (MQ=255)
                                                          gaagGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCt                                                                                                                                   >  1:498756/1‑104 (MQ=255)
                                                                cGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACggt                                                                                            >  3:908224/1‑137 (MQ=255)
                                                                cGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACggt                                                                                            <  4:908224/137‑1 (MQ=255)
                                                                          ccTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTAGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGGAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCaa                                                                       >  3:740373/1‑148 (MQ=255)
                                                                                tCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGACAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGa                                                                 <  4:740373/148‑1 (MQ=255)
                                                                                 cAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTgg                                                                                                                                 <  3:665243/83‑1 (MQ=255)
                                                                                 cAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTgg                                                                                                                                 >  4:665243/1‑83 (MQ=255)
                                                                                                       cTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGAt                                                                                                                                                  <  4:1041840/44‑1 (MQ=255)
                                                                                                       cTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGAt                                                                                                                                                  >  3:1041840/1‑44 (MQ=255)
                                                                                                           tGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGa                                                                 <  1:1070870/121‑1 (MQ=255)
                                                                                                           tGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGa                                                                 >  2:1070870/1‑121 (MQ=255)
                                                                                                             gggCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTg                                    >  3:610653/1‑148 (MQ=255)
                                                                                                               gCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGAc                                                                                                                             <  3:533168/57‑1 (MQ=255)
                                                                                                               gCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGAc                                                                                                                             >  4:533168/1‑57 (MQ=255)
                                                                                                                   gCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTa                              >  4:447276/1‑148 (MQ=255)
                                                                                                                    cAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTAt                             <  4:610653/148‑1 (MQ=255)
                                                                                                                       aaTTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATggg                          <  3:447276/148‑1 (MQ=255)
                                                                                                                         ttattaCTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTgg                                             <  1:910226/127‑1 (MQ=255)
                                                                                                                         ttattaCTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTgg                                             >  2:910226/1‑127 (MQ=255)
                                                                                                                          tattaCTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCaa                                        >  4:1050858/1‑131 (MQ=255)
                                                                                                                          tattaCTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCaa                                        <  3:1050858/131‑1 (MQ=255)
                                                                                                                             taCTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACgg                                                                                             <  3:328135/75‑1 (MQ=255)
                                                                                                                             taCTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACgg                                                                                             >  4:328135/1‑75 (MQ=255)
                                                                                                                             taCTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATtg                                                              >  2:162510/1‑106 (MQ=255)
                                                                                                                             taCTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATtg                                                              <  1:162510/106‑1 (MQ=255)
                                                                                                                                 cAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTa                                                                                                                      >  3:453579/1‑46 (MQ=255)
                                                                                                                                 cAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTa                                                                                                                      <  4:453579/46‑1 (MQ=255)
                                                                                                                                 cAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAgg                                                                             >  1:429884/1‑87 (MQ=255)
                                                                                                                                 cAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAgg                                                                             <  2:429884/87‑1 (MQ=255)
                                                                                                                                 cAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTg                >  1:774672/1‑148 (MQ=255)
                                                                                                                                   aGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATc                                                                                                               <  3:503639/51‑1 (MQ=255)
                                                                                                                                   aGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATc                                                                                                               >  4:503639/1‑51 (MQ=255)
                                                                                                                                   aGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCTATtgtg                                                            <  4:288621/102‑1 (MQ=255)
                                                                                                                                   aGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATtgtg                                                            >  3:288621/1‑102 (MQ=255)
                                                                                                                                   aGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGc              >  4:515246/1‑148 (MQ=255)
                                                                                                                                   aGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGc              >  2:952850/1‑148 (MQ=255)
                                                                                                                                     ccccTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAg                                                                                                       >  1:199940/1‑57 (MQ=255)
                                                                                                                                     ccccTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAg                                                                                                       <  2:199940/57‑1 (MQ=255)
                                                                                                                                        cTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATtg                                                              >  1:531051/1‑95 (MQ=255)
                                                                                                                                        cTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATtg                                                              <  2:531051/95‑1 (MQ=255)
                                                                                                                                         tGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAaga        <  3:515246/148‑1 (MQ=255)
                                                                                                                                            aGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAgg                                                                             >  3:384876/1‑76 (MQ=255)
                                                                                                                                            aGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAgg                                                                             <  4:384876/76‑1 (MQ=255)
                                                                                                                                            aGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATtg                                                              >  1:660165/1‑91 (MQ=255)
                                                                                                                                            aGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATtg                                                              <  2:660165/91‑1 (MQ=255)
                                                                                                                                             ggggATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCGAAAGGGCAAAACGGTGGTCCTTGACTTAg                                                                              >  1:819599/1‑74 (MQ=255)
                                                                                                                                             ggggATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCGAAAGGGCAAAACGGTGGTCCTTGACTTAg                                                                              <  2:819599/74‑1 (MQ=255)
                                                                                                                                             ggggATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTg                                                                                    >  3:661686/1‑68 (MQ=255)
                                                                                                                                             ggggATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATtg                                                              >  1:269317/1‑90 (MQ=255)
                                                                                                                                             ggggATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATtg                                                              <  2:269317/90‑1 (MQ=255)
                                                                                                                                             gggaATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTg                                                                                    <  4:661686/68‑1 (MQ=255)
                                                                                                                                              gggATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTa                                                                               <  2:1061713/72‑1 (MQ=255)
                                                                                                                                              gggATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTa                                                                               >  1:1061713/1‑72 (MQ=255)
                                                                                                                                              gggATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTg                                    >  1:774798/1‑115 (MQ=255)
                                                                                                                                              gggATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTg                                    <  2:774798/115‑1 (MQ=255)
                                                                                                                                              gggATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAggtgg   >  1:105299/1‑148 (MQ=255)
                                                                                                                                               ggATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTc                                                                   >  1:913251/1‑83 (MQ=255)
                                                                                                                                               ggATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTc                                                                   <  2:913251/83‑1 (MQ=255)
                                                                                                                                               ggATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCa                                         <  2:1041586/109‑1 (MQ=255)
                                                                                                                                               ggATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCa                                         >  1:1041586/1‑109 (MQ=255)
                                                                                                                                               ggATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAggtggt  >  1:1002724/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGT  >  NC_000913/1147848‑1148138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑