| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,177,646 | T→G | 5.5% | N109K (AAT→AAG) | lolE → | lipoprotein‑releasing system transmembrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,177,646 | 0 | T | G | 5.5% | 265.6 / 7.1 | 73 | N109K (AAT→AAG) | lolE | lipoprotein‑releasing system transmembrane protein |
| Reads supporting (aligned to +/- strand): ref base T (35/34); new base G (2/2); total (37/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.08e-01 | |||||||||||
GCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGC > NC_000913/1177500‑1177789 | gCGGTGGTGCCGCTTGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATc > 3:78287/1‑148 (MQ=255)gCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATc > 3:687934/1‑148 (MQ=255) ggtggtGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCtt < 2:1085826/148‑1 (MQ=255) gtgCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCtt < 1:236485/144‑1 (MQ=255) gtgCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCtt > 2:236485/1‑144 (MQ=255) aaTCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAg < 3:1141435/148‑1 (MQ=255) aTCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAgg > 4:700057/1‑148 (MQ=255) tCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAggg < 3:700057/148‑1 (MQ=255) aTCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATcc < 3:177368/126‑1 (MQ=255) aTCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATcc > 4:177368/1‑126 (MQ=255) cGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGa > 3:265231/1‑148 (MQ=255) gACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAg < 4:687934/148‑1 (MQ=255) gACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAg < 4:78287/148‑1 (MQ=255) ggCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGACTCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAg < 4:265231/148‑1 (MQ=255) ggAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGt > 1:1060962/1‑117 (MQ=255) ggAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGt < 2:1060962/117‑1 (MQ=255) aaGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTaa < 4:837164/118‑1 (MQ=255) aaGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTaa > 3:837164/1‑118 (MQ=255) cTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAgcg > 4:733080/1‑139 (MQ=255) cTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAgcg < 3:733080/139‑1 (MQ=255) tGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCg > 1:291870/1‑107 (MQ=255) tGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCg < 2:291870/107‑1 (MQ=255) tGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGt > 3:53839/1‑122 (MQ=255) tGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGt < 4:53839/122‑1 (MQ=255) aaaaGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCa < 2:768355/105‑1 (MQ=255) aaaaGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCa > 1:768355/1‑105 (MQ=255) gggTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCg < 4:170409/127‑1 (MQ=255) gggTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCg > 3:170409/1‑127 (MQ=255) ggTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGTATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAg < 3:698759/99‑1 (MQ=255) ggTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTcg < 3:167661/65‑1 (MQ=255) ggTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTcg > 4:167661/1‑65 (MQ=255) ggTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCCACAg > 4:698759/1‑99 (MQ=255) tATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTcc < 1:804274/131‑1 (MQ=255) tATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTcc > 2:804274/1‑131 (MQ=255) ccgctgccgcGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCTTCGTTTGTCCAGGGcca < 4:761945/133‑3 (MQ=255) ccgctgccgcGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGa > 3:761945/1‑133 (MQ=255) cgctgccgcGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCg < 2:752848/136‑1 (MQ=255) cgctgccgcGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCg > 1:752848/1‑136 (MQ=255) ccGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCt > 3:600171/1‑106 (MQ=255) ccGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCt < 4:600171/106‑1 (MQ=255) cGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGa > 2:480461/1‑148 (MQ=255) atatCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGt > 4:971336/1‑123 (MQ=255) atatCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGACCGCATTACCCTCGTTTGTCCAGGGCGATGCGt < 3:971336/123‑1 (MQ=255) atCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCAtt > 2:487768/1‑95 (MQ=255) atCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCAtt < 1:487768/95‑1 (MQ=255) tCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGa < 4:821014/54‑1 (MQ=255) tCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGa > 3:821014/1‑54 (MQ=255) tCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCgg < 2:914/138‑1 (MQ=255) tCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCgg > 1:914/1‑138 (MQ=255) cAATTTCACCGGGCTGGTGGAAGGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCg > 1:647512/1‑59 (MQ=255) cAATTTCACCGGGCTGGTGGAAGGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCg < 2:647512/59‑1 (MQ=255) aTTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCg < 2:97167/111‑1 (MQ=255) aTTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCg > 1:97167/1‑111 (MQ=255) ttCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGt > 2:907696/1‑78 (MQ=255) ttCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAgcg > 2:446553/1‑85 (MQ=255) ttCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAgcg < 1:446553/85‑1 (MQ=255) ttCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAgg < 2:318355/106‑1 (MQ=255) ttCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAgg > 1:318355/1‑106 (MQ=255) ttCACCGGGCTGGGGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGt < 1:907696/78‑1 (MQ=255) ggtggAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGa > 1:46200/1‑126 (MQ=255) ggtggAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGa < 2:46200/126‑1 (MQ=255) gAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCg > 1:962238/1‑148 (MQ=255) tgcgcGAAGATTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCa > 1:51193/2‑83 (MQ=255) tgcgcGAAGATTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCa > 1:286421/2‑83 (MQ=255) tgcgcGAAGATTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCa < 2:286421/82‑1 (MQ=255) tgcgcGAAGATTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCa < 2:51193/82‑1 (MQ=255) cgAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCg < 1:95017/71‑1 (MQ=255) cgAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCg > 2:95017/1‑71 (MQ=255) cgAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATgc > 2:1029680/1‑148 (MQ=255) gAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAg < 1:867789/57‑1 (MQ=255) gAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAg > 2:867789/1‑57 (MQ=255) aTCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTatc > 1:837676/1‑122 (MQ=255) aTCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTatc < 2:837676/122‑1 (MQ=255) | GCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGC > NC_000913/1177500‑1177789 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |