| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,231,963 | T→A | 6.1% | Y260N (TAT→AAT) | umuC → | translesion error‑prone DNA polymerase V subunit; DNA polymerase activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,231,963 | 0 | T | A | 6.1% | 239.5 / 5.3 | 66 | Y260N (TAT→AAT) | umuC | translesion error‑prone DNA polymerase V subunit; DNA polymerase activity |
| Reads supporting (aligned to +/- strand): ref base T (30/32); new base A (2/2); total (32/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01 | |||||||||||
ATCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGCG > NC_000913/1231816‑1232107 | atCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGAtt > 3:1144957/1‑148 (MQ=255)atCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGAATC‑ACGGAtt > 2:936928/1‑148 (MQ=255) ccGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATg < 4:1144957/148‑1 (MQ=255) gTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCg > 1:998911/1‑148 (MQ=255) tttAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCGACGGATAATCCGtga < 2:76293/136‑3 (MQ=255) tttAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCGACGGATAATCCGtga > 1:76293/1‑134 (MQ=255) gTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGa > 3:918017/1‑130 (MQ=255) gTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGa < 4:918017/130‑1 (MQ=255) aaaGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCg < 3:309656/116‑1 (MQ=255) aaaGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCg > 4:309656/1‑116 (MQ=255) aaaGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGc > 3:549712/1‑148 (MQ=255) aaCTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGaaaa < 2:998911/148‑1 (MQ=255) aCTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGaaaaa < 1:638963/148‑1 (MQ=255) aCTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGaaaaa < 1:936928/148‑1 (MQ=255) cTGTTTTCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTg > 2:614879/1‑105 (MQ=255) cTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTg < 1:614879/105‑1 (MQ=255) cTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTg < 1:23974/105‑1 (MQ=255) cTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTg > 2:23974/1‑105 (MQ=255) gTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGt > 3:492882/1‑104 (MQ=255) gTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGt < 4:492882/104‑1 (MQ=255) gTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCa < 4:549712/148‑1 (MQ=255) gAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCa > 4:487726/1‑83 (MQ=255) gAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCa < 3:487726/83‑1 (MQ=255) aaGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGaa > 1:403819/1‑116 (MQ=255) aaGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGaa < 2:403819/116‑1 (MQ=255) gagTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGaaaaa < 1:1099972/117‑1 (MQ=255) gagTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGaaaaa > 2:1099972/1‑117 (MQ=255) agTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGtt < 2:553284/145‑1 (MQ=255) agTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGtt > 1:553284/1‑145 (MQ=255) gCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGc < 3:958525/81‑1 (MQ=255) gCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGc > 4:958525/1‑81 (MQ=255) ttATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCg < 4:317338/118‑1 (MQ=255) ttATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCg > 3:317338/1‑118 (MQ=255) tCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACg < 3:1089559/69‑1 (MQ=255) tCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACg > 4:1089559/1‑69 (MQ=255) tCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGc > 4:358266/1‑90 (MQ=255) tCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGc < 3:358266/90‑1 (MQ=255) cTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACtt < 4:736294/82‑1 (MQ=255) cTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACtt > 3:736294/1‑82 (MQ=255) ttGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAAcc > 3:131026/1‑148 (MQ=255) gTGAACGCATC‑ACGGATTATCCGTCGTTGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCa > 4:819216/1‑85 (MQ=255) gTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCa < 3:819216/85‑1 (MQ=255) tGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTa > 4:142741/1‑44 (MQ=255) tGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTa < 3:142741/44‑1 (MQ=255) tGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGtt < 1:805080/111‑1 (MQ=255) tGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGtt > 2:805080/1‑111 (MQ=255) cATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACg < 4:886315/115‑1 (MQ=255) cATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACg > 3:886315/1‑115 (MQ=255) cATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGaa < 2:758412/136‑1 (MQ=255) cATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGaa > 1:758412/1‑136 (MQ=255) cATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGc > 2:508383/1‑148 (MQ=255) atcgaCGGATAATCCGT‑GATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCa > 1:160995/5‑148 (MQ=255) tC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACtt < 4:1101353/65‑1 (MQ=255) tC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACtt > 3:1101353/1‑65 (MQ=255) c‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCg < 4:592563/71‑1 (MQ=255) c‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCg > 3:592563/1‑71 (MQ=255) c‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGtt > 4:1133332/1‑102 (MQ=255) c‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGtt < 3:1133332/102‑1 (MQ=255) c‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTttat < 2:433414/105‑1 (MQ=255) c‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTttat > 1:433414/1‑105 (MQ=255) c‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCAttt > 1:128310/1‑121 (MQ=255) c‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCAttt < 2:128310/121‑1 (MQ=255) c‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCAtt < 2:130152/120‑1 (MQ=255) c‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCAtt > 1:130152/1‑120 (MQ=255) cGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTgcg > 3:852042/1‑122 (MQ=255) cGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTgcg < 4:852042/122‑1 (MQ=255) cGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAg < 4:131026/148‑1 (MQ=255) ggATAATCCGT‑GATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAgcg < 2:160995/148‑1 (MQ=255) tATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGaa < 1:308820/126‑1 (MQ=255) tATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGaa > 2:308820/1‑126 (MQ=255) | ATCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGCG > NC_000913/1231816‑1232107 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |