Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,248,064 T→A 7.7% K351* (AAA→TAA)  dhaM ← putative dihydroxyacetone‑specific PTS enzymes: HPr, EI components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,248,0640TA 7.7% 367.7 / 15.1 104K351* (AAA→TAA) dhaMputative dihydroxyacetone‑specific PTS enzymes: HPr, EI components
Reads supporting (aligned to +/- strand):  ref base T (49/47);  new base A (4/4);  total (53/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 108 total aligned reads displayed.

GAGTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTT  >  NC_000913/1247931‑1248211
                                                                                                                                     |                                                                                                                                                   
                  tcttctTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGc                                                                                                                     >  4:748123/1‑148 (MQ=255)
                     tcttTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAAc                                                                                                                                               >  2:1102326/1‑119 (MQ=255)
                     tcttTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAAc                                                                                                                                               <  1:1102326/119‑1 (MQ=255)
                     tcttTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAAt                                                                                                                  >  1:216984/1‑148 (MQ=255)
                       tttCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGcc                                                                                                                                       <  3:856238/125‑1 (MQ=255)
                       tttCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGcc                                                                                                                                       >  4:856238/1‑125 (MQ=255)
                       tttCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTAAAGaa                                                                                                                                                <  1:417842/116‑1 (MQ=255)
                        ttCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGtt                                                                                                               >  1:1043713/1‑148 (MQ=255)
                             ttGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGc                                                                                                                          <  4:1055454/132‑1 (MQ=255)
                                    aGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGa                                                                                                       >  2:259294/1‑144 (MQ=255)
                                    aGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGa                                                                                                       <  1:259294/144‑1 (MQ=255)
                                      gTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCa                                                                                                             <  3:653291/136‑1 (MQ=255)
                                      gTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCa                                                                                                             >  4:653291/1‑136 (MQ=255)
                                       tGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCa                                                                                                                                      >  4:1095345/1‑110 (MQ=255)
                                       tGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCa                                                                                                                                      <  3:1095345/110‑1 (MQ=255)
                                       tGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTAAAGAACtta                                                                                                                                            <  3:65391/104‑2 (MQ=255)
                                       tGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTAAAGAACtta                                                                                                                                            >  4:65391/1‑103 (MQ=255)
                                         gACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCg                                                                                              <  2:216984/148‑1 (MQ=255)
                                          aCCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTa                                                                                                                                                    >  3:509348/1‑93 (MQ=255)
                                           ccAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCt                                                                                            <  3:748123/148‑1 (MQ=255)
                                             aGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGa                                                                                                       <  1:530304/135‑1 (MQ=255)
                                             aGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGa                                                                                                       >  2:530304/1‑135 (MQ=255)
                                                           aagaTCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGa                                                                                                                                                 >  4:312503/1‑79 (MQ=255)
                                                           aagaTCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGa                                                                                                                                                 <  3:312503/79‑1 (MQ=255)
                                                            agaTCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGtt                                                                           >  3:351284/1‑148 (MQ=255)
                                                                  tCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGATCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGat                                                                     <  2:1043713/148‑1 (MQ=255)
                                                                   ccACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAGCTTGCTGCCAGGCATAtt                                                                                                                              <  4:950359/90‑1 (MQ=255)
                                                                   ccACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATAtt                                                                                                                              >  3:950359/1‑90 (MQ=255)
                                                                      caTCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTg                                                                                                                           <  4:52212/90‑1 (MQ=255)
                                                                      caTCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGat                                                                     >  2:603001/1‑144 (MQ=255)
                                                                      caTCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGat                                                                     <  1:603001/144‑1 (MQ=255)
                                                                          aaTATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAAc                                                             <  3:1008636/148‑1 (MQ=255)
                                                                           atatAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACa                                                            >  2:280760/1‑148 (MQ=255)
                                                                                 cGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGat                                                                     <  4:1103361/133‑1 (MQ=255)
                                                                                 cGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGat                                                                     >  3:1103361/1‑133 (MQ=255)
                                                                                  gAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATg                                                     >  3:965557/1‑148 (MQ=255)
                                                                                   aGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATgg                                                    >  4:465371/1‑148 (MQ=255)
                                                                                         tAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAgg                                                                                                    <  3:701905/94‑1 (MQ=255)
                                                                                         tAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAgg                                                                                                    >  4:701905/1‑94 (MQ=255)
                                                                                         tAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTc                                                                                               >  4:410859/1‑99 (MQ=255)
                                                                                         tAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTc                                                                                               <  3:410859/99‑1 (MQ=255)
                                                                                            atatTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTAAAGAACtt                                                                                                                                             >  2:137855/1‑50 (MQ=255)
                                                                                            atatTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTAAAGAACtt                                                                                                                                             <  1:137855/50‑1 (MQ=255)
                                                                                             tatTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTgc                                                                                         <  2:249393/101‑1 (MQ=255)
                                                                                             tatTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTgc                                                                                         >  1:249393/1‑101 (MQ=255)
                                                                                             tatTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGaaa                                          <  4:351284/148‑1 (MQ=255)
                                                                                             tatTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGaaa                                          >  1:563137/1‑148 (MQ=255)
                                                                                               ttCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGtt                                                                                                <  4:1104737/92‑1 (MQ=255)
                                                                                               ttCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGtt                                                                                                >  3:1104737/1‑92 (MQ=255)
                                                                                               ttCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTAt                                                      <  4:508108/134‑1 (MQ=255)
                                                                                               ttCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTAt                                                      >  3:508108/1‑134 (MQ=255)
                                                                                                tcatcaTCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGcc                                                                                                                                       <  4:145695/52‑1 (MQ=255)
                                                                                                tcatcaTCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGcc                                                                                                                                       >  3:145695/1‑52 (MQ=255)
                                                                                                   tcatcCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCt                                                                                            >  2:42881/1‑92 (MQ=255)
                                                                                                   tcatcCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCt                                                                                            <  1:42881/92‑1 (MQ=255)
                                                                                                   tcatcCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGatca                                                                   >  3:162227/1‑117 (MQ=255)
                                                                                                   tcatcCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGatca                                                                   <  4:162227/117‑1 (MQ=255)
                                                                                                    catcCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTg                                                  <  2:642300/133‑1 (MQ=255)
                                                                                                    catcCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTg                                                  >  1:642300/1‑133 (MQ=255)
                                                                                                    catcCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGc                                   <  3:465371/148‑1 (MQ=255)
                                                                                                    catcCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGc                                   <  1:280760/148‑1 (MQ=255)
                                                                                                      tcCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTgc                                                                                         <  2:4987/92‑1 (MQ=255)
                                                                                                      tcCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTgc                                                                                         >  1:4987/1‑92 (MQ=255)
                                                                                                      tcCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGa                                       <  3:975614/142‑1 (MQ=255)
                                                                                                       ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCt                                                                                            <  2:189872/88‑1 (MQ=255)
                                                                                                       ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCt                                                                                            >  1:189872/1‑88 (MQ=255)
                                                                                                       ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAgtgt                                                        >  3:1111099/1‑124 (MQ=255)
                                                                                                       ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAgtgt                                                        <  4:1111099/124‑1 (MQ=255)
                                                                                                       ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGAc                                                >  1:162186/1‑132 (MQ=255)
                                                                                                       ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGAc                                                <  2:162186/132‑1 (MQ=255)
                                                                                                        cAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCTGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCg                                  <  1:1061533/145‑1 (MQ=255)
                                                                                                        cAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCg                                  >  2:1061533/1‑145 (MQ=255)
                                                                                                        cAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGCTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAGAGATCGCGGCa                               <  4:965557/148‑1 (MQ=255)
                                                                                                            tGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCa                                                                                                             <  3:799168/66‑1 (MQ=255)
                                                                                                            tGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCa                                                                                                             >  4:799168/1‑66 (MQ=255)
                                                                                                                 gTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCa                      >  2:634724/1‑148 (MQ=255)
                                                                                                                   aTTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCaa                              <  3:917151/138‑1 (MQ=255)
                                                                                                                   aTTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCaa                              >  4:917151/1‑138 (MQ=255)
                                                                                                                     tGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGcc                  >  2:756592/1‑148 (MQ=255)
                                                                                                                        tGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCt                                                                                            <  2:760460/71‑1 (MQ=255)
                                                                                                                          gCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTg             <  1:634724/148‑1 (MQ=255)
                                                                                                                           cTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCa                      >  1:1072686/1‑138 (MQ=255)
                                                                                                                           cTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCa                      <  2:1072686/138‑1 (MQ=255)
                                                                                                                            tAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCa                      >  2:654192/1‑137 (MQ=255)
                                                                                                                            tAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCa                      <  1:654192/137‑1 (MQ=255)
                                                                                                                             aaGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTgg            <  3:421714/146‑1 (MQ=255)
                                                                                                                             aaGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTgg            >  4:421714/1‑146 (MQ=255)
                                                                                                                             aaGTTCTTAAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCt                                                                                            >  4:776441/1‑66 (MQ=255)
                                                                                                                             aaGTTCTTAAAGAACTTGCGGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCt                                                                                            <  3:776441/66‑1 (MQ=255)
                                                                                                                              aGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCa                      <  2:554337/135‑1 (MQ=255)
                                                                                                                              aGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCa                      >  1:554337/1‑135 (MQ=255)
                                                                                                                                 tCTTTAAGAACTTGCTGCCAGGCATATTTTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCa                      <  4:791116/132‑1 (MQ=255)
                                                                                                                                 tCTTTAAGAACTTGCTGCCAGGCATATTTTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCa                      >  3:791116/1‑132 (MQ=255)
                                                                                                                                 tCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAg                    <  1:209726/134‑1 (MQ=255)
                                                                                                                                 tCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAg                    >  2:209726/1‑134 (MQ=255)
                                                                                                                                 tCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTg      >  1:612716/1‑148 (MQ=255)
                                                                                                                                    ttAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGa                                       <  1:246056/112‑1 (MQ=255)
                                                                                                                                    ttAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGa                                       >  2:246056/1‑112 (MQ=255)
                                                                                                                                     tAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCttt  <  1:756592/148‑1 (MQ=255)
                                                                                                                                     tAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCttt  <  2:563137/148‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                                   
GAGTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTT  >  NC_000913/1247931‑1248211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: