Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,303,657 T→A 6.4% I253I (ATT→ATA oppB → oligopeptide ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,303,6570TA 6.4% 334.1 / 9.7 96I253I (ATT→ATAoppBoligopeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (44/45);  new base A (3/3);  total (48/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01

TATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACC  >  NC_000913/1303513‑1303802
                                                                                                                                                |                                                                                                                                                 
tATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTAt                                                                                                                                                <  2:1103403/148‑1 (MQ=255)
   tCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGt                                                                                                                                             <  1:1092127/148‑1 (MQ=255)
                                        aTCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCgg                                                                                                                                      <  3:303935/118‑1 (MQ=255)
                                        aTCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCgg                                                                                                                                      >  4:303935/1‑118 (MQ=255)
                                         tCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCAtt                                                                                                       >  2:506267/1‑148 (MQ=255)
                                             tttACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTaa                                                                                                                <  2:158703/135‑1 (MQ=255)
                                             tttACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTaa                                                                                                                >  1:158703/1‑135 (MQ=255)
                                             tttACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAAc                                                                                                   >  1:8041/1‑148 (MQ=255)
                                                    cACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATAAATGGttt                                                                                                                                           >  3:437485/1‑101 (MQ=255)
                                                    cACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATAAATGGttt                                                                                                                                           <  4:437485/101‑1 (MQ=255)
                                                     aCGCATTAGAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTa                                                                                           >  4:981521/1‑148 (MQ=255)
                                                       gCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTAtt                                                                                         <  2:8041/148‑1 (MQ=255)
                                                           tAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTg                                                                                               <  2:1110472/138‑1 (MQ=255)
                                                           tAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTg                                                                                               >  1:1110472/1‑138 (MQ=255)
                                                           tAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCtt                                                                                     <  1:506267/148‑1 (MQ=255)
                                                             aaaCCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCAtt                                                                                                       <  3:337326/128‑1 (MQ=255)
                                                             aaaCCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCAtt                                                                                                       >  4:337326/1‑128 (MQ=255)
                                                             aaaCCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATCGCGCATTGAACCGTGACTATTACATAg                                                                                   >  4:734854/1‑148 (MQ=255)
                                                                ccTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAgtgt                                                                                >  1:750301/1‑148 (MQ=255)
                                                                    ctctGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATg                                                                                                                                               >  3:1048082/1‑81 (MQ=255)
                                                                    ctctGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATg                                                                                                                                               <  4:1048082/81‑1 (MQ=255)
                                                                        gTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTg                                                                                                                        <  2:990270/100‑1 (MQ=255)
                                                                        gTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTg                                                                                                                        >  1:990270/1‑100 (MQ=255)
                                                                              cGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGAc                                                                                             >  3:39189/1‑121 (MQ=255)
                                                                              cGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGAc                                                                                             <  4:39189/121‑1 (MQ=255)
                                                                              cGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATAAATGGtttc                                                                                                                                          >  3:965109/1‑75 (MQ=255)
                                                                              cGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATAAATGGtttc                                                                                                                                          <  4:965109/76‑2 (MQ=255)
                                                                                tGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTc                                                                                        <  2:891222/124‑1 (MQ=255)
                                                                                tGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTc                                                                                        >  1:891222/1‑124 (MQ=255)
                                                                                 gCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGAt                                                                                                                                  <  1:33062/81‑1 (MQ=255)
                                                                                 gCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGAt                                                                                                                                  >  2:33062/1‑81 (MQ=255)
                                                                                 gCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCg                                                                                                 >  1:785490/1‑114 (MQ=255)
                                                                                 gCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCg                                                                                                 <  2:785490/114‑1 (MQ=255)
                                                                                   tctctTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGTCCATCCtggt                                                             <  3:734854/148‑1 (MQ=255)
                                                                                    ctctTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCtt                                                                                     <  1:575246/123‑1 (MQ=255)
                                                                                    ctctTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCtt                                                                                     >  2:575246/1‑123 (MQ=255)
                                                                                    ctctTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCtggtt                                                            >  1:776026/1‑148 (MQ=255)
                                                                                    ctctTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATAAATTGtttc                                                                                                                                          <  4:953760/70‑2 (MQ=255)
                                                                                    ctctTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATAAATGGtttc                                                                                                                                          >  3:953760/1‑69 (MQ=255)
                                                                                        tataTGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTg                                                                                               >  4:845041/1‑109 (MQ=255)
                                                                                        tataTGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTg                                                                                               <  3:845041/109‑1 (MQ=255)
                                                                                        tataTGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTg                                                        >  1:63396/1‑148 (MQ=255)
                                                                                         atatGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGc                                                       <  2:63396/148‑1 (MQ=255)
                                                                                           atGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATgg                                                                                                                                              <  1:734411/59‑1 (MQ=255)
                                                                                           atGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATgg                                                                                                                                              >  2:734411/1‑59 (MQ=255)
                                                                                           atGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAg                                                                                   <  1:199161/118‑1 (MQ=255)
                                                                                           atGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAg                                                                                   >  2:199161/1‑118 (MQ=255)
                                                                                             gggCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCtggttg                                                           <  3:383022/140‑1 (MQ=255)
                                                                                             gggCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCtggttg                                                           >  4:383022/1‑140 (MQ=255)
                                                                                               gCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCg                                                                                                                    >  3:649413/1‑81 (MQ=255)
                                                                                               gCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCg                                                                                                                    <  4:649413/81‑1 (MQ=255)
                                                                                                  ccGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGAt                                                                                                                                  <  4:543416/64‑1 (MQ=255)
                                                                                                  ccGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGAt                                                                                                                                  >  3:543416/1‑64 (MQ=255)
                                                                                                  ccGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCACTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCAt                                              <  3:981521/148‑1 (MQ=255)
                                                                                                       tttGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTTAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGt                                         >  4:350913/1‑148 (MQ=255)
                                                                                                       tttGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGt                                         <  3:350913/148‑1 (MQ=255)
                                                                                                        ttGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTaa                                                                                                                <  1:297764/76‑1 (MQ=255)
                                                                                                        ttGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTaa                                                                                                                >  2:297764/1‑76 (MQ=255)
                                                                                                          gTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTa                                      <  2:776026/148‑1 (MQ=255)
                                                                                                           tCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTaa                                     <  2:750301/148‑1 (MQ=255)
                                                                                                              gCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATg                                   <  4:874084/147‑1 (MQ=255)
                                                                                                              gCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATg                                   >  3:874084/1‑147 (MQ=255)
                                                                                                               cATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCg                                                                                                 <  4:473909/84‑1 (MQ=255)
                                                                                                               cATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCg                                                                                                 >  3:473909/1‑84 (MQ=255)
                                                                                                                 ttattaCCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTc                                                                                        <  4:826629/91‑1 (MQ=255)
                                                                                                                 ttattaCCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTc                                                                                        >  3:826629/1‑91 (MQ=255)
                                                                                                                     taCCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGcc                                                                          <  1:1110119/101‑1 (MQ=255)
                                                                                                                     taCCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGcc                                                                          >  2:1110119/1‑101 (MQ=255)
                                                                                                                       ccGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTGGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGcc                                 <  2:977214/140‑1 (MQ=255)
                                                                                                                       ccGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGa                         >  3:101593/1‑148 (MQ=255)
                                                                                                                       ccGGTTCTATGGTTATCGAAACCATGTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGcc                                 >  1:977214/1‑140 (MQ=255)
                                                                                                                        cGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCtt                                                                                     <  4:804059/87‑1 (MQ=255)
                                                                                                                        cGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCtt                                                                                     >  3:804059/1‑87 (MQ=255)
                                                                                                                        cGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCtggt                                                             <  1:1119180/111‑1 (MQ=255)
                                                                                                                        cGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCtggt                                                             >  2:1119180/1‑111 (MQ=255)
                                                                                                                        cGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGa                         <  2:1016707/147‑1 (MQ=255)
                                                                                                                        cGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGa                         >  1:1016707/1‑147 (MQ=255)
                                                                                                                           ttCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTaa                                     <  1:334598/132‑1 (MQ=255)
                                                                                                                           ttCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTaa                                     >  2:334598/1‑132 (MQ=255)
                                                                                                                                  gTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATg              >  3:71578/1‑148 (MQ=255)
                                                                                                                                   ttATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCAttt                                            <  4:586557/117‑1 (MQ=255)
                                                                                                                                   ttATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCAttt                                            >  3:586557/1‑117 (MQ=255)
                                                                                                                                    tATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTa                                      >  1:580192/1‑122 (MQ=255)
                                                                                                                                    tATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTa                                      <  2:580192/122‑1 (MQ=255)
                                                                                                                                    tATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTc                           >  1:652031/1‑133 (MQ=255)
                                                                                                                                    tATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTc                           <  2:652031/133‑1 (MQ=255)
                                                                                                                                       cGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCtggttg                                                           >  1:502952/1‑98 (MQ=255)
                                                                                                                                       cGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCtggttg                                                           <  2:502952/98‑1 (MQ=255)
                                                                                                                                       cGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGTTTTa                                                   <  4:672590/106‑1 (MQ=255)
                                                                                                                                       cGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTa                                                   >  3:672590/1‑106 (MQ=255)
                                                                                                                                         aaaCCATTTATGGTTTGCCGGGGATTGGACAGCTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCAtt                                             <  2:1080152/110‑1 (MQ=255)
                                                                                                                                         aaaCCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTg                                                                                               <  1:906863/60‑1 (MQ=255)
                                                                                                                                         aaaCCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTg                                                                                               >  2:906863/1‑60 (MQ=255)
                                                                                                                                         aaaCCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCAtt                                             >  1:1080152/1‑110 (MQ=255)
                                                                                                                                          aaCCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAg                                                                                   >  2:268761/1‑71 (MQ=255)
                                                                                                                                          aaCCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAg                                                                                   <  1:268761/71‑1 (MQ=255)
                                                                                                                                               tttATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGt                                         <  4:682204/108‑1 (MQ=255)
                                                                                                                                               tttATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGt                                         >  3:682204/1‑108 (MQ=255)
                                                                                                                                               tttATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGAcc  <  4:52877/147‑1 (MQ=255)
                                                                                                                                               tttATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGAcc  >  3:52877/1‑147 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
TATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACC  >  NC_000913/1303513‑1303802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: