| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,324,839 | G→T | 20.4% | E32* (GAA→TAA) | yciQ → | enhancer of membrane protein expression; putative inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,324,839 | 0 | G | T | 20.4% | 139.0 / 23.9 | 50 | E32* (GAA→TAA) | yciQ | enhancer of membrane protein expression; putative inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (19/20); new base T (6/4); total (25/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAG > NC_000913/1324703‑1324982 | gAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGa < 1:117897/146‑1 (MQ=255)gAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGa > 2:117897/1‑146 (MQ=255) aaGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATgaaga < 2:930147/140‑1 (MQ=255) aaGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATgaaga > 1:930147/1‑140 (MQ=255) ataACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGAt < 1:49384/148‑1 (MQ=255) aaCAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATgaaga < 2:267712/127‑1 (MQ=255) aaCAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATgaaga > 1:267712/1‑127 (MQ=255) aaCAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGt > 1:907657/1‑148 (MQ=255) cAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑CATAAGAAgg > 2:863867/1‑128 (MQ=255) tCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAgg > 4:876060/1‑118 (MQ=255) tCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAgg < 3:876060/118‑1 (MQ=255) aCGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTg > 2:910227/1‑129 (MQ=255) aCGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTg < 1:910227/129‑1 (MQ=255) cGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATgaaga < 3:972495/111‑1 (MQ=255) cGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATgaaga > 4:972495/1‑111 (MQ=255) cGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGaagaag < 1:165962/113‑1 (MQ=255) cGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGaagaag > 2:165962/1‑113 (MQ=255) gACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATgaagaa > 2:178990/1‑103 (MQ=255) gACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATgaagaa < 1:178990/103‑1 (MQ=255) gggAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑CATAAGAAgg < 1:935020/95‑1 (MQ=255) gggAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCGCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑CATAAGAAgg > 2:935020/1‑95 (MQ=255) tttCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTCAT‑AAGAAGGGa < 2:631831/91‑1 (MQ=39) tttCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑CATAAGAAGGGa > 1:631831/1‑91 (MQ=255) aTAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGaa > 4:492996/1‑90 (MQ=255) aTAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGaa < 3:492996/90‑1 (MQ=255) gggCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAgg > 4:420449/1‑139 (MQ=255) gggCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAgg < 3:420449/139‑1 (MQ=255) tGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGa > 2:675447/1‑114 (MQ=255) tGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGa < 1:675447/114‑1 (MQ=255) tAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATgg > 2:335930/1‑85 (MQ=255) tAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATgg < 1:335930/85‑1 (MQ=255) aaaCACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTa > 3:121752/1‑148 (MQ=255) cGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCt > 1:1101765/1‑73 (MQ=255) cGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCt < 2:1101765/73‑1 (MQ=255) gagaTCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATcag > 1:264783/1‑111 (MQ=255) gagaTCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATcag < 2:264783/111‑1 (MQ=255) gaTCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGAc < 2:750058/73‑1 (MQ=255) gaTCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGAc > 1:750058/1‑73 (MQ=255) gaTCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTa < 2:907657/148‑1 (MQ=255) tCCCTTCTT‑CATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATg < 3:182333/148‑1 (MQ=255) tCCCTTCTT‑CATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATg > 4:182333/1‑148 (MQ=255) cccTTCTT‑CATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAAt < 2:1084540/103‑1 (MQ=255) cccTTCTT‑CATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAAt > 1:1084540/1‑103 (MQ=255) cccTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCaa < 3:550614/75‑1 (MQ=255) cccTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCaa > 4:550614/1‑75 (MQ=255) ccTTCTT‑CATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCtttt > 3:399829/1‑124 (MQ=255) ccTTCTT‑CATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCtttt < 4:399829/124‑1 (MQ=255) ccTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATca > 3:612690/1‑104 (MQ=255) ccTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATca < 4:612690/104‑1 (MQ=255) ccTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTg > 1:86277/1‑130 (MQ=255) ccTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTg < 2:86277/130‑1 (MQ=255) ccTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATgg < 2:547834/147‑1 (MQ=255) ccTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATgg > 1:547834/1‑147 (MQ=255) ttctt‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCttt > 4:1115738/1‑121 (MQ=255) ttctt‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCttt < 3:1115738/121‑1 (MQ=255) ctt‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCCGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTc < 3:375387/87‑1 (MQ=255) ctt‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCCGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTc > 4:375387/1‑87 (MQ=255) tt‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAg < 4:121752/148‑1 (MQ=255) | GAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAG > NC_000913/1324703‑1324982 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |